TMEM194A ORF clone pFN21ASDA0286

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Halotag ORF Clone pFN21ASDA0286

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Clone info.

Species

human

Clone Name

pFN21ASDA0286 Sequence (with vector) Restriction Map

Product ID

FHC00495

Flexi Clone PID

FXC00495

ORF Sequence

DNA sequence (1332 bp)
Protein sequence (444 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB383838]

Gene

KIAA0286

Symbol

TMEM194A

Description

nuclear envelope integral membrane protein 1, transcript variant 1

Original Clone ID

ha06800s1

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1332 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 444 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS44927.1 1.6e-190 100.0 NEMP1 CCDS31841.1 7.1e-111 83.6 NEMP1 CCDS46476.1 2.2e-52 34.9 NEMP2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR019358 151 399 PF10225 Transmembrane protein 194
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 26 GTVRLLLILSGCLVYGTAETDVN 48 SECONDARY 23 2 159 FDPKLFLVFLLGLMLFFCGDLLS 181 PRIMARY 23 3 190 TGMTVGIVASLLIIIFILSKFMP 212 PRIMARY 23 4 218 YVILVGGWSFSLYLIQLVFKNLQ 240 PRIMARY 23 5 248 QYLLSYVLTVGFMSFAVCYKYG 269 PRIMARY 22 6 285 QLMGLCFMYSGIQIPHIALAIII 307 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001124435 444 616496 100.0 100.0 XP_011536359 452 616496 100.0 90.8 XP_016874566 452 616496 100.0 90.8 XP_011536358 492 616496 92.2 99.8 NP_056072 371 616496 83.6 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0286 Download

>KIAA0286  1332 bp
ATGGCGGGAGGAATGAAAGTGGCGGTCTCGCCGGCAGTTGGTCCCGGGCCCTGGGGCTCG
GGAGTCGGGGGCGGTGGGACAGTGCGGCTACTCTTGATCCTCTCCGGCTGCTTGGTCTAC
GGCACAGCTGAAACTGATGTAAATGTGGTCATGCTTCAGGAATCCCAAGTTTGTGAAAAG
CGTGCCAGCCAACAATTCTGTTACACAAATGTGCTTATCCCAAAATGGCATGATATATGG
ACACGGATACAGATCCGAGTAAATAGTTCCAGATTGGTTCGAGTCACCCAGGTGGAGAAT
GAGGAGAAACTGAAGGAGCTAGAGCAGTTTAGTATCTGGAACTTTTTTTCCTCCTTTTTA
AAAGAGAAATTGAATGACACCTATGTTAACGTGGGTCTATACAGCACAAAAACCTGCCTC
AAAGTTGAGATTATAGAGAAGGACACCAAGTACAGTGTCATTGTGATCCGGAGATTTGAT
CCCAAACTCTTTCTTGTTTTCCTTCTTGGACTTATGCTATTTTTTTGTGGAGACTTGCTG
AGCAGAAGTCAAATTTTCTACTACTCTACTGGGATGACTGTGGGAATTGTGGCCTCTCTG
CTAATCATCATTTTTATACTATCTAAGTTTATGCCTAAGAAAAGTCCCATTTACGTCATC
CTGGTGGGAGGCTGGTCTTTTTCTCTGTACCTCATTCAACTAGTTTTTAAAAATTTACAA
GAGATCTGGAGGTGTTACTGGCAGTATCTTTTAAGTTATGTCCTCACAGTTGGATTCATG
AGTTTTGCAGTATGTTACAAGTATGGGCCCTTGGAGAATGAACGAAGTATCAACCTGCTG
ACCTGGACCTTGCAGCTGATGGGCCTGTGTTTCATGTATTCTGGCATCCAGATACCACAT
ATTGCCCTTGCCATTATCATCATTGCTCTTTGTACTAAGAACCTGGAACACCCTATTCAG
TGGCTGTACATCACCTGCAGAAAGGTGTGTAAGGGAGCAGAAAAGCCTGTTCCCCCTCGT
CTCCTGACAGAAGAAGAATATCGGATACAAGGAGAGGTAGAAACCCGAAAGGCTTTAGAG
GAGCTCCGAGAATTTTGTAACAGTCCAGACTGCTCTGCTTGGAAGACTGTTTCTCGAATC
CAGTCTCCAAAAAGATTTGCTGACTTTGTGGAAGGCTCTTCCCACCTCACGCCAAATGAA
GTTTCTGTCCATGAGCAGGAGTATGGATTAGGGAGCATTATTGCCCAGGATGAAATCTAT
GAGGAAGCATCCTCTGAGGAGGAGGACTCATATTCTCGGTGTCCTGCTATCACACAGAAC
AACTTTCTAACC

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0286 Download

>KIAA0286  444 aa
MAGGMKVAVSPAVGPGPWGSGVGGGGTVRLLLILSGCLVYGTAETDVNVVMLQESQVCEK
RASQQFCYTNVLIPKWHDIWTRIQIRVNSSRLVRVTQVENEEKLKELEQFSIWNFFSSFL
KEKLNDTYVNVGLYSTKTCLKVEIIEKDTKYSVIVIRRFDPKLFLVFLLGLMLFFCGDLL
SRSQIFYYSTGMTVGIVASLLIIIFILSKFMPKKSPIYVILVGGWSFSLYLIQLVFKNLQ
EIWRCYWQYLLSYVLTVGFMSFAVCYKYGPLENERSINLLTWTLQLMGLCFMYSGIQIPH
IALAIIIIALCTKNLEHPIQWLYITCRKVCKGAEKPVPPRLLTEEEYRIQGEVETRKALE
ELREFCNSPDCSAWKTVSRIQSPKRFADFVEGSSHLTPNEVSVHEQEYGLGSIIAQDEIY
EEASSEEEDSYSRCPAITQNNFLT

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0286 Download

>pFN21ASDA0286  6061 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGCGGGAGGAATGAA
AGTGGCGGTCTCGCCGGCAGTTGGTCCCGGGCCCTGGGGCTCGGGAGTCGGGGGCGGTGG
GACAGTGCGGCTACTCTTGATCCTCTCCGGCTGCTTGGTCTACGGCACAGCTGAAACTGA
TGTAAATGTGGTCATGCTTCAGGAATCCCAAGTTTGTGAAAAGCGTGCCAGCCAACAATT
CTGTTACACAAATGTGCTTATCCCAAAATGGCATGATATATGGACACGGATACAGATCCG
AGTAAATAGTTCCAGATTGGTTCGAGTCACCCAGGTGGAGAATGAGGAGAAACTGAAGGA
GCTAGAGCAGTTTAGTATCTGGAACTTTTTTTCCTCCTTTTTAAAAGAGAAATTGAATGA
CACCTATGTTAACGTGGGTCTATACAGCACAAAAACCTGCCTCAAAGTTGAGATTATAGA
GAAGGACACCAAGTACAGTGTCATTGTGATCCGGAGATTTGATCCCAAACTCTTTCTTGT
TTTCCTTCTTGGACTTATGCTATTTTTTTGTGGAGACTTGCTGAGCAGAAGTCAAATTTT
CTACTACTCTACTGGGATGACTGTGGGAATTGTGGCCTCTCTGCTAATCATCATTTTTAT
ACTATCTAAGTTTATGCCTAAGAAAAGTCCCATTTACGTCATCCTGGTGGGAGGCTGGTC
TTTTTCTCTGTACCTCATTCAACTAGTTTTTAAAAATTTACAAGAGATCTGGAGGTGTTA
CTGGCAGTATCTTTTAAGTTATGTCCTCACAGTTGGATTCATGAGTTTTGCAGTATGTTA
CAAGTATGGGCCCTTGGAGAATGAACGAAGTATCAACCTGCTGACCTGGACCTTGCAGCT
GATGGGCCTGTGTTTCATGTATTCTGGCATCCAGATACCACATATTGCCCTTGCCATTAT
CATCATTGCTCTTTGTACTAAGAACCTGGAACACCCTATTCAGTGGCTGTACATCACCTG
CAGAAAGGTGTGTAAGGGAGCAGAAAAGCCTGTTCCCCCTCGTCTCCTGACAGAAGAAGA
ATATCGGATACAAGGAGAGGTAGAAACCCGAAAGGCTTTAGAGGAGCTCCGAGAATTTTG
TAACAGTCCAGACTGCTCTGCTTGGAAGACTGTTTCTCGAATCCAGTCTCCAAAAAGATT
TGCTGACTTTGTGGAAGGCTCTTCCCACCTCACGCCAAATGAAGTTTCTGTCCATGAGCA
GGAGTATGGATTAGGGAGCATTATTGCCCAGGATGAAATCTATGAGGAAGCATCCTCTGA
GGAGGAGGACTCATATTCTCGGTGTCCTGCTATCACACAGAACAACTTTCTAACCTACGT
AGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
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