(Circular) (Six-base) MAPSORT of: pFN21ASDA0286.seq Check: 8269 from: 1 to: 6061 pFN21ASDA0286 6061 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3005 3005 Size: 6061 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3871 278 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2139 3404 1937 Size: 202 1265 4594 Fragments arranged by size: 4594 1265 202 Acc65I G'GTAC_C Cuts at: 3382 3382 Size: 6061 AclI AA'CG_TT Cuts at: 1563 4188 4561 1563 Size: 2625 373 3063 Fragments arranged by size: 3063 2625 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2162 2354 4124 5508 5776 1632 Size: 530 192 1770 1384 268 1917 Fragments arranged by size: 1917 1770 1384 530 268 192 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5864 AflIII A'CryG_T Cuts at: 1203 4960 5937 1203 Size: 3757 977 1327 Fragments arranged by size: 3757 1327 977 AgeI A'CCGG_T Cuts at: 4892 4892 Size: 6061 AhdI GACnn_n'nnGTC Cuts at: 4790 4790 Size: 6061 AleI CACnn'nnGTG Cuts at: 1166 2231 2312 1166 Size: 1065 81 4915 Fragments arranged by size: 4915 1065 81 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6061 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2354 2386 2354 Size: 32 6029 AlwNI CAG_nnn'CTG Cuts at: 2986 3804 5376 2986 Size: 818 1572 3671 Fragments arranged by size: 3671 1572 818 ApaI G_GGCC'C Cuts at: 2073 2832 2073 Size: 759 5302 ApaLI G'TGCA_C Cuts at: 4118 5274 4118 Size: 1156 4905 ApoI r'AATT_y Cuts at: 1493 2574 2734 3113 3370 3596 3748 5857 1493 Size: 1081 160 379 257 226 152 2109 1697 Fragments arranged by size: 2109 1697 1081 379 257 226 160 152 AseI AT'TA_AT Cuts at: 160 1032 4615 160 Size: 872 3583 1606 Fragments arranged by size: 3583 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 AvaI C'yCGr_G Cuts at: 1870 1943 2066 2080 3386 1870 Size: 73 123 14 1306 4545 Fragments arranged by size: 4545 1306 123 73 14 BamHI G'GATC_C Cuts at: 3391 3758 3391 Size: 367 5694 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3382 4837 618 Size: 325 217 627 1595 1455 1842 Fragments arranged by size: 1842 1595 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2073 2081 2832 3108 3380 1391 Size: 682 8 751 276 272 4072 Fragments arranged by size: 4072 751 682 276 272 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3152 928 Size: 971 1253 3837 Fragments arranged by size: 3837 1253 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4252 4286 1903 Size: 34 2315 34 3678 Fragments arranged by size: 3678 2315 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2262 3060 3953 5163 2262 Size: 798 893 1210 3160 Fragments arranged by size: 3160 1210 893 798 BclI T'GATC_A Cuts at: 1548 1548 Size: 6061 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4672 136 Size: 107 122 71 631 605 3000 1525 Fragments arranged by size: 3000 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2745 6056 2745 Size: 3311 2750 BlpI GC'TnA_GC Cuts at: 2561 3478 2561 Size: 917 5144 Bme1580I G_kGCm'C Cuts at: 1790 2073 2832 4122 5278 1790 Size: 283 759 1290 1156 2573 Fragments arranged by size: 2573 1290 1156 759 283 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6061 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2601 3792 4750 Size: 416 53 83 399 24 1184 1191 958 Cuts at: 4750 442 Size: 1753 Fragments arranged by size: 1753 1191 1184 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6061 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3325 3325 Size: 6061 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2770 4721 1239 Size: 1531 1951 2579 Fragments arranged by size: 2579 1951 1531 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4186 5051 5349 4186 Size: 865 298 4898 Fragments arranged by size: 4898 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2053 4724 882 Size: 617 554 2671 2219 Fragments arranged by size: 2671 2219 617 554 BsaAI yAC'GTr Cuts at: 493 1204 3358 3852 493 Size: 711 2154 494 2702 Fragments arranged by size: 2702 2154 711 494 BsaBI GATnn'nnATC Cuts at: 2273 6055 2273 Size: 3782 2279 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3868 4250 4885 275 Size: 53 83 186 936 2335 382 635 1451 Fragments arranged by size: 2335 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2490 4494 4892 5166 5313 5724 2490 Size: 2004 398 274 147 411 2827 Fragments arranged by size: 2827 2004 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5836 Fragments arranged by size: 5836 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2339 3116 3314 3317 1186 Size: 1153 777 198 3 3930 Fragments arranged by size: 3930 1153 777 198 3 BseYI C'CCAG_C Cuts at: 5264 5760 5264 Size: 496 5565 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5488 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3511 4272 5300 1540 Size: 275 186 1510 761 1028 2301 Fragments arranged by size: 2301 1510 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3108 4122 4207 5278 3108 Size: 1014 85 1071 3891 Fragments arranged by size: 3891 1071 1014 85 BsmI GAATG_Cn' Cuts at: 3572 3665 3572 Size: 93 5968 BsmBI CGTCTCn'nnnn_ Cuts at: 3047 3047 Size: 6061 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2081 2832 3108 3380 4122 4207 Size: 399 283 8 751 276 272 742 85 Cuts at: 4207 5278 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 751 742 399 283 276 272 85 8 BspEI T'CCGG_A Cuts at: 2490 5724 2490 Size: 3234 2827 BspHI T'CATG_A Cuts at: 2799 3950 5680 2799 Size: 1151 1730 3180 Fragments arranged by size: 3180 1730 1151 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2444 2865 3005 3416 844 Size: 1600 421 140 411 3489 Fragments arranged by size: 3489 1600 421 411 140 BsrBI CCG'CTC Cuts at: 1394 1823 3948 1394 Size: 429 2125 3507 Fragments arranged by size: 3507 2125 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2943 4556 4730 65 Size: 1183 1695 1613 174 1396 Fragments arranged by size: 1695 1613 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2053 4705 4892 1700 Size: 110 243 2652 187 2869 Fragments arranged by size: 2869 2652 243 187 110 BsrGI T'GTAC_A Cuts at: 96 2988 96 Size: 2892 3169 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6061 BssSI C'ACGA_G Cuts at: 4121 5133 4121 Size: 1012 5049 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6061 BstBI TT'CG_AA Cuts at: 2007 4865 2007 Size: 2858 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6061 BstYI r'GATC_y Cuts at: 1972 2274 2745 3391 3758 4143 4160 5612 5698 Size: 302 471 646 367 385 17 1452 86 Cuts at: 5698 5710 6056 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 646 471 385 367 346 302 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4552 Fragments arranged by size: 4552 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5322 Fragments arranged by size: 5322 683 56 BtsI GCAGTG_nn' Cuts at: 3577 4371 4391 3577 Size: 794 20 5247 Fragments arranged by size: 5247 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6061 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6026 DraI TTT'AAA Cuts at: 2382 2731 3365 2382 Size: 349 634 5078 Fragments arranged by size: 5078 634 349 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5068 809 Size: 507 3752 1802 Fragments arranged by size: 3752 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3508 4397 6026 Size: 54 1425 50 275 18 1678 889 1629 Cuts at: 6026 8 Size: 43 Fragments arranged by size: 1678 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3508 1537 Size: 275 1696 4090 Fragments arranged by size: 4090 1696 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3205 3991 1353 Size: 1852 786 3423 Fragments arranged by size: 3423 1852 786 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4648 5022 5168 387 Size: 1064 138 3059 374 146 1280 Fragments arranged by size: 3059 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3106 3106 Size: 6061 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2162 2354 2770 4124 4721 5508 5776 Size: 393 530 192 416 1354 597 787 268 Cuts at: 5776 1239 Size: 1524 Fragments arranged by size: 1524 1354 787 597 530 416 393 268 192 EcoO109I rG'GnC_Cy Cuts at: 2070 2829 2070 Size: 759 5302 EcoRI G'AATT_C Cuts at: 3370 3748 3370 Size: 378 5683 FspI TGC'GCA Cuts at: 4567 4567 Size: 6061 HaeII r_GCGC'y Cuts at: 1678 5208 1678 Size: 3530 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2485 2517 2554 2586 3139 Size: 32 135 32 913 32 37 32 553 Cuts at: 3139 3171 3275 3307 4702 4734 4776 4808 1373 Size: 32 104 32 1395 32 42 32 2626 Fragments arranged by size: 2626 1395 913 553 135 104 42 37 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2410 3405 3651 669 Size: 988 281 472 995 246 3079 Fragments arranged by size: 3079 995 988 472 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6061 HpaI GTT'AAC Cuts at: 2410 3651 2410 Size: 1241 4820 KpnI G_GTAC'C Cuts at: 3386 3386 Size: 6061 MfeI C'AATT_G Cuts at: 3660 3660 Size: 6061 MluI A'CGCG_T Cuts at: 5937 5937 Size: 6061 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2773 5175 5359 1305 Size: 1468 2402 184 2007 Fragments arranged by size: 2402 2007 1468 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4239 Fragments arranged by size: 4239 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2231 2312 2684 4019 4378 4537 518 Size: 648 1065 81 372 1335 359 159 2042 Fragments arranged by size: 2042 1335 1065 648 372 359 159 81 MspA1I CmG'CkG Cuts at: 1606 2150 2878 3477 4154 5302 5547 1606 Size: 544 728 599 677 1148 245 2120 Fragments arranged by size: 2120 1148 728 677 599 544 245 NaeI GCC'GGC Cuts at: 2055 2055 Size: 6061 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4552 Fragments arranged by size: 4552 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6061 NgoMIV G'CCGG_C Cuts at: 2053 2053 Size: 6061 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6061 NotI GC'GGCC_GC Cuts at: 3508 3508 Size: 6061 NspI r_CATG'y Cuts at: 1132 1229 3419 4964 1132 Size: 97 2190 1545 2229 Fragments arranged by size: 2229 2190 1545 97 PasI CC'CwG_GG Cuts at: 1348 2073 1348 Size: 725 5336 PciI A'CATG_T Cuts at: 4960 4960 Size: 6061 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6061 PmeI GTTT'AAAC Cuts at: 3365 3365 Size: 6061 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4130 4162 5643 5675 4130 Size: 32 1481 32 4516 Fragments arranged by size: 4516 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6061 PsiI TTA'TAA Cuts at: 3631 3631 Size: 6061 PspOMI G'GGCC_C Cuts at: 2069 2828 2069 Size: 759 5302 PstI C_TGCA'G Cuts at: 830 3002 3413 830 Size: 2172 411 3478 Fragments arranged by size: 3478 2172 411 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6061 PvuII CAG'CTG Cuts at: 2150 2878 2150 Size: 728 5333 SacI G_AGCT'C Cuts at: 3108 3108 Size: 6061 SalI G'TCGA_C Cuts at: 1936 3403 1936 Size: 1467 4594 SapI GCTCTTCn'nnn_ Cuts at: 3205 3205 Size: 6061 SbfI CC_TGCA'GG Cuts at: 3413 3413 Size: 6061 ScaI AGT'ACT Cuts at: 4309 6021 4309 Size: 1712 4349 SfcI C'TryA_G Cuts at: 826 1045 2998 3409 4544 5225 5416 826 Size: 219 1953 411 1135 681 191 1471 Fragments arranged by size: 1953 1471 1135 681 411 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6061 SmaI CCC'GGG Cuts at: 1872 2068 3388 1872 Size: 196 1320 4545 Fragments arranged by size: 4545 1320 196 SmlI C'TyrA_G Cuts at: 820 1017 1943 4165 5066 5328 820 Size: 197 926 2222 901 262 1553 Fragments arranged by size: 2222 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3358 3852 493 Size: 2865 494 2702 Fragments arranged by size: 2865 2702 494 SpeI A'CTAG_T Cuts at: 152 2722 152 Size: 2570 3491 SphI G_CATG'C Cuts at: 1132 3419 1132 Size: 2287 3774 SspI AAT'ATT Cuts at: 5 52 3985 5 Size: 47 3933 2081 Fragments arranged by size: 3933 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6061 StyI C'CwwG_G Cuts at: 513 1065 2022 2832 3500 513 Size: 552 957 810 668 3074 Fragments arranged by size: 3074 957 810 668 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2324 4088 4105 4258 4443 4888 325 Size: 1255 744 1764 17 153 185 445 1498 Fragments arranged by size: 1764 1498 1255 744 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2472 2954 2988 Size: 274 80 33 51 1443 495 482 34 Cuts at: 2988 4307 6019 96 Size: 1319 1712 138 Fragments arranged by size: 1712 1443 1319 495 482 274 138 80 51 34 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2025 2057 6021 6053 2025 Size: 32 3964 32 2033 Fragments arranged by size: 3964 2033 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6061 XbaI T'CTAG_A Cuts at: 3397 3397 Size: 6061 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6061 XmaI C'CCGG_G Cuts at: 1870 2066 3386 1870 Size: 196 1320 4545 Fragments arranged by size: 4545 1320 196 XmnI GAAnn'nnTTC Cuts at: 4190 4190 Size: 6061 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3869 276 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI