KIAA0286

Product ID	ORK00495
Accession No	AB006624
Description	nuclear envelope integral membrane protein 1, transcript variant 1
Clone name	ha06800s1
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pSPORT1 vector (GenBank Accession No. U12390, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5577 bp) Predicted protein sequence (446 aa)
HaloTag ORF Clone	FHC00495
Flexi ORF Clone	FXC00495
Source	Human adult brain
Rouge ID	mKIAA0286 by Kazusa Mouse cDNA Project
Note	We replaced ha06800, former representative clones for KIAA0286 with ha06800s1. (2002/5/10)

Length: 5577 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	4235 bp
Genome contig ID	gi89161190r_55635694
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- TTTCTAATTCCAATTTTAATAAAAGTTTTATAGAT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATATTGTGGTCTTTCTTAATGAACATAATATTTTCTTGTTTGGATTTCTT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	12	r	55735694	55758802	9	99.2	Perfect prediction

Length: 446 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
AAH71597	2.6e-189	100.0	TMEM194A protei...	Homo sapiens
Q5RDB4	5.4e-185	98.0	Transmembrane p...	Pongo abelii
XP_001099369	5.7e-183	97.1	hypothetical pr...	Macaca mulatta
A7MBC7	1.3e-161	85.4	Transmembrane p...	Bos taurus
EDM16455	1.7e-154	83.1	rCG59551 [Rattu...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1	ATMAGGMKVAVSPAVGPGPWGSG	23	SECONDARY	23
	2	28	GTVRLLLILSGCLVYGTAETDVN	50	SECONDARY	23
	3	161	FDPKLFLVFLLGLMLFFCGDLLS	183	PRIMARY	23
	4	192	TGMTVGIVASLLIIIFILSKFMP	214	PRIMARY	23
	5	220	YVILVGGWSFSLYLIQLVFKNLQ	242	PRIMARY	23
	6	250	QYLLSYVLTVGFMSFAVCYKYG	271	PRIMARY	22
	7	287	QLMGLCFMYSGIQIPHIALAIII	309	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Genebridge 4
Primer_f	GATGTTGGTGCCTTATGTGAC
Primer_r	ACTCCCATCTGCCACTAAAGC
PCR product length	99 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles