GPR132 ORF clone pFN21AE9570

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Halotag ORF Clone pFN21AE9570

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Clone info.

Species

human

Clone Name

pFN21AE9570 Sequence (with vector) Restriction Map

Product ID

FHC25312

ORF Sequence

DNA sequence (1140 bp)
Protein sequence (380 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9570

Symbol

GPR132

Alias : G2A

Description

G protein-coupled receptor 132, transcript variant 2

Original Clone ID

cp99570

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1140 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 380 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9997.1 1.5e-143 100.0 GPR132 CCDS61567.1 1.1e-138 100.0 GPR132 CCDS12669.1 2.3e-33 38.8 GPR4 CCDS9894.2 2.6e-28 29.7 GPR68 CCDS2148.1 1.7e-20 27.4 GPR17 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 44 68 PR00237 GPCR IPR000276 77 98 PR00237 GPCR IPR005388 100 110 PR01563 G2A lysophosphatidylcholine receptor IPR000276 122 144 PR00237 GPCR IPR005388 145 157 PR01563 G2A lysophosphatidylcholine receptor IPR005388 165 175 PR01563 G2A lysophosphatidylcholine receptor IPR005388 193 202 PR01563 G2A lysophosphatidylcholine receptor IPR000276 200 223 PR00237 GPCR IPR005388 216 227 PR01563 G2A lysophosphatidylcholine receptor IPR000276 240 264 PR00237 GPCR IPR005388 265 275 PR01563 G2A lysophosphatidylcholine receptor IPR000276 290 316 PR00237 GPCR IPR005388 312 333 PR01563 G2A lysophosphatidylcholine receptor IPR005388 357 368 PR01563 G2A lysophosphatidylcholine receptor HMMPfam IPR000276 60 308 PF00001 GPCR ProfileScan IPR017452 59 308 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 43 IVLVVVYSAVCTLGVPANCLTAW 65 PRIMARY 23 2 78 AVYLLCLALCELLYTGTLPLWVI 100 PRIMARY 23 3 118 TAYIFFCNIYVSILFLCCISCDR 140 PRIMARY 23 4 159 AILISACIFILVGIVHYPVFQT 180 PRIMARY 22 5 197 AGYYYARFTVGFAIPLSIIAFTN 219 SECONDARY 23 6 242 HSAIAVVVIFLVCFAPYHLVLLV 264 PRIMARY 23 7 289 ASVVFLCLSTVNGVADPIIYVLA 311 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_037477 380 606167 100.0 100.0 NP_001265623 380 606167 100.0 100.0 NP_001265624 371 606167 100.0 97.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9570 Download

>KIEE9570  1140 bp
ATGTGCCCAATGCTACTGAAAAACGGTTACAATGGAAACGCCACCCCAGTGACCACCACT
GCCCCGTGGGCCTCCCTGGGCCTCTCCGCCAAGACCTGCAACAACGTGTCCTTCGAAGAG
AGCAGGATAGTCCTGGTAGTGGTGTACAGCGCGGTGTGCACGCTGGGGGTGCCGGCCAAC
TGCCTGACTGCGTGGCTGGCGCTGCTGCAGGTACTGCAGGGCAACGTGCTGGCCGTCTAC
CTGCTCTGCCTGGCACTCTGCGAGCTGCTGTACACAGGCACGCTGCCACTCTGGGTCATC
TATATCCGCAACCAGCACCGCTGGACCCTAGGCCTGCTGGCCTGCAAGGTGACCGCCTAC
ATCTTCTTCTGCAACATCTACGTCAGCATCCTCTTCCTGTGCTGCATCTCCTGCGACCGC
TTCGTGGCCGTGGTGTACGCGCTGGAGAGTCGGGGCCGCCGCCGCCGGAGGACCGCCATC
CTCATCTCCGCCTGCATCTTCATCCTCGTCGGGATCGTTCACTACCCGGTGTTCCAGACG
GAAGACAAGGAGACCTGCTTTGACATGCTGCAGATGGACAGCAGGATTGCCGGGTACTAC
TACGCCAGGTTCACCGTTGGCTTTGCCATCCCTCTCTCCATCATCGCCTTCACCAACCAC
CGGATTTTCAGGAGCATCAAGCAGAGCATGGGCTTAAGCGCTGCCCAGAAGGCCAAGGTG
AAGCACTCGGCCATCGCGGTGGTTGTCATCTTCCTAGTCTGCTTCGCCCCGTACCACCTG
GTTCTCCTCGTCAAAGCCGCTGCCTTTTCCTACTACAGAGGAGACAGGAACGCCATGTGC
GGCTTGGAGGAAAGGCTGTACACAGCCTCTGTGGTGTTTCTGTGCCTGTCCACGGTGAAC
GGCGTGGCTGACCCCATTATCTACGTGCTGGCCACGGACCATTCCCGCCAAGAAGTGTCC
AGAATCCATAAGGGGTGGAAAGAGTGGTCCATGAAGACAGACGTCACCAGGCTCACCCAC
AGCAGGGACACCGAGGAGCTGCAGTCGCCCGTGGCCCTTGCAGACCACTACACCTTCTCC
AGGCCCGTGCACCCACCAGGGTCACCATGCCCTGCAAAGAGGCTGATTGAGGAGTCCTGC

ORF Protein

Cloned ORF protein sequence for pFN21AE9570 Download

>KIEE9570  380 aa
MCPMLLKNGYNGNATPVTTTAPWASLGLSAKTCNNVSFEESRIVLVVVYSAVCTLGVPAN
CLTAWLALLQVLQGNVLAVYLLCLALCELLYTGTLPLWVIYIRNQHRWTLGLLACKVTAY
IFFCNIYVSILFLCCISCDRFVAVVYALESRGRRRRRTAILISACIFILVGIVHYPVFQT
EDKETCFDMLQMDSRIAGYYYARFTVGFAIPLSIIAFTNHRIFRSIKQSMGLSAAQKAKV
KHSAIAVVVIFLVCFAPYHLVLLVKAAAFSYYRGDRNAMCGLEERLYTASVVFLCLSTVN
GVADPIIYVLATDHSRQEVSRIHKGWKEWSMKTDVTRLTHSRDTEELQSPVALADHYTFS
RPVHPPGSPCPAKRLIEESC

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9570 Download

>pFN21AE9570  5845 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTGCCCAATGCTACTGAAAAACGGTTACAATGG
AAACGCCACCCCAGTGACCACCACTGCCCCGTGGGCCTCCCTGGGCCTCTCCGCCAAGAC
CTGCAACAACGTGTCCTTCGAAGAGAGCAGGATAGTCCTGGTAGTGGTGTACAGCGCGGT
GTGCACGCTGGGGGTGCCGGCCAACTGCCTGACTGCGTGGCTGGCGCTGCTGCAGGTACT
GCAGGGCAACGTGCTGGCCGTCTACCTGCTCTGCCTGGCACTCTGCGAGCTGCTGTACAC
AGGCACGCTGCCACTCTGGGTCATCTATATCCGCAACCAGCACCGCTGGACCCTAGGCCT
GCTGGCCTGCAAGGTGACCGCCTACATCTTCTTCTGCAACATCTACGTCAGCATCCTCTT
CCTGTGCTGCATCTCCTGCGACCGCTTCGTGGCCGTGGTGTACGCGCTGGAGAGTCGGGG
CCGCCGCCGCCGGAGGACCGCCATCCTCATCTCCGCCTGCATCTTCATCCTCGTCGGGAT
CGTTCACTACCCGGTGTTCCAGACGGAAGACAAGGAGACCTGCTTTGACATGCTGCAGAT
GGACAGCAGGATTGCCGGGTACTACTACGCCAGGTTCACCGTTGGCTTTGCCATCCCTCT
CTCCATCATCGCCTTCACCAACCACCGGATTTTCAGGAGCATCAAGCAGAGCATGGGCTT
AAGCGCTGCCCAGAAGGCCAAGGTGAAGCACTCGGCCATCGCGGTGGTTGTCATCTTCCT
AGTCTGCTTCGCCCCGTACCACCTGGTTCTCCTCGTCAAAGCCGCTGCCTTTTCCTACTA
CAGAGGAGACAGGAACGCCATGTGCGGCTTGGAGGAAAGGCTGTACACAGCCTCTGTGGT
GTTTCTGTGCCTGTCCACGGTGAACGGCGTGGCTGACCCCATTATCTACGTGCTGGCCAC
GGACCATTCCCGCCAAGAAGTGTCCAGAATCCATAAGGGGTGGAAAGAGTGGTCCATGAA
GACAGACGTCACCAGGCTCACCCACAGCAGGGACACCGAGGAGCTGCAGTCGCCCGTGGC
CCTTGCAGACCACTACACCTTCTCCAGGCCCGTGCACCCACCAGGGTCACCATGCCCTGC
AAAGAGGCTGATTGAGGAGTCCTGCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT