################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## Acc65I AccIII AclI AcsI AgeI AhdI AocI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BglII BlpI Bpu1102I BsaBI BseAI BsgI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO109I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tsp509I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 1000 GACGTC GACGT^C AccI 235 GTCTAC GT^[AC][GT]AC AcyI 1000 GACGTC G[GA]^CG[CT]C AflII 693 CTTAAG C^TTAAG AflIII 104 ACGTGT A^C[GA][CT]GT AlwI 512 GGATC GGATC(4/5) Aor51HI 697 AGCGCT AGC^GCT AvrII 327 CCTAGG C^CTAGG BalI 929 TGGCCA TGG^CCA BanI 168 GGTGCC G^G[CT][GA]CC BfrI 693 CTTAAG C^TTAAG BlnI 327 CCTAGG C^CTAGG BsaAI 922 TACGTG [CT]AC^GT[GA] BsaHI 1000 GACGTC G[GA]^CG[CT]C BsaI 550 GAGACC GGTCTC(1/5) BsaWI 659 ACCGGA [AT]^CCGG[AT] BsiEI 414 CGACCG CG[GA][CT]^CG BsmFI 1025 GGGAC GGGAC(10/14) BsrFI 171 GCCGGC [GA]^CCGG[CT] BsrI 46 CCAGT ACTGG(1/-1) BstBI 112 TTCGAA TT^CGAA BstXI 731 CCATCGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 171 GCCGGC [GA]^CCGG[CT] DpnII 513 GATC ^GATC Eco47III 697 AGCGCT AGC^GCT EcoNI 1073 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 944 CCCGC CCCGC(4/6) Hin1I 1000 GACGTC G[GA]^CG[CT]C MboI 513 GATC ^GATC MluNI 929 TGGCCA TGG^CCA MscI 929 TGGCCA TGG^CCA MseI 694 TTAA T^TAA NaeI 171 GCCGGC GCC^GGC NdeII 513 GATC ^GATC NgoMIV 171 GCCGGC G^CCGGC NspI 563 ACATGC [GA]CATG^[CT] NspV 112 TTCGAA TT^CGAA PshAI 996 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Sau3AI 513 GATC ^GATC SexAI 776 ACCTGGT A^CC[AT]GGT SfuI 112 TTCGAA TT^CGAA SmlI 693 CTTAAG C^T[CT][GA]AG StuI 330 AGGCCT AGG^CCT TaqI 113 TCGA T^CGA TfiI 962 GAATC G^A[AT]TC Tru9I 694 TTAA T^TAA TthHB8I 113 TCGA T^CGA XcmI 833 CCATGTGCGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AluI 263 AGCT AG^CT 1037 AGCT Alw44I 156 GTGCAC G^TGCAC 1087 GTGCAC AlwNI 208 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG 863 CAGCCTCTG ApaLI 156 GTGCAC G^TGCAC 1087 GTGCAC AspHI 156 GTGCAC G[AT]GC[AT]^C 1087 GTGCAC BbsI 541 GAAGAC GAAGAC(2/6) 993 GAAGAC BcnI 525 CCCGG CC^[GC]GG 590 CCGGG BfaI 328 CTAG C^TAG 754 CTAG BglI 61 GCCCCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 703 GCCCAGAAGGC BpmI 1077 CTCCAG CTGGAG(16/14) 442 CTGGAG BpuAI 541 GAAGAC GAAGAC(2/6) 993 GAAGAC BsiHKAI 156 GTGCAC G[AT]GC[AT]^C 1087 GTGCAC BsmAI 550 GAGAC GTCTC(1/5) 821 GAGAC BstAPI 207 GCAGGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 404 GCATCTCCTGC BstEII 348 GGTGACC G^GT[ACGT]ACC 1100 GGTCACC BstPI 348 GGTGACC G^GT[ACGT]ACC 1100 GGTCACC EarI 391 CTCTTC CTCTTC(1/4) 115 GAAGAG EcoO65I 348 GGTGACC G^GT[ACGT]ACC 1100 GGTCACC EcoT14I 327 CCTAGG C^C[AT][AT]GG 713 CCAAGG HaeII 198 GGCGCT [GA]GCGC^[CT] 697 AGCGCT Ksp632I 391 CTCTTC CTCTTC(1/4) 115 GAAGAG MaeI 328 CTAG C^TAG 754 CTAG MslI 42 CACCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 732 CATCGCGGTG MspA1I 318 CCGCTG C[AC]G^C[GT]G 797 CCGCTG NciI 525 CCCGG CC^[GC]GG 590 CCGGG PleI 447 GAGTC GAGTC(4/5) 1132 GAGTC StyI 327 CCTAGG C^C[AT][AT]GG 713 CCAAGG TspRI 47 CAGTG CA[GC]TG[ACGT][ACGT]^ 57 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AccII AciI AfaI AvaII BbvI BmyI BsaJI BseRI BsiYI BslI Bsp1286I Bsp1407I BspMI BsrGI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DsaI EaeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboII MnlI MspI MvaI MvnI MwoI NlaIII NlaIV PstI RsaI Sau96I ScrFI SfaNI SfcI SspBI TseI Tsp45I