(Circular) (Six-base) MAPSORT of: pFN21AE9570.seq Check: 6749 from: 1 to: 5845 pFN21AE9570 5845 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3009 3655 278 Size: 53 83 186 936 1473 646 2468 Fragments arranged by size: 2468 1473 936 646 186 83 53 AccI GT'mk_AC Cuts at: 1937 2241 3188 1937 Size: 304 947 4594 Fragments arranged by size: 4594 947 304 Acc65I G'GTAC_C Cuts at: 3166 3166 Size: 5845 AclI AA'CG_TT Cuts at: 1563 3972 4345 1563 Size: 2409 373 3063 Fragments arranged by size: 3063 2409 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3908 5292 5560 1632 Size: 2276 1384 268 1917 Fragments arranged by size: 2276 1917 1384 268 AfeI AGC'GCT Cuts at: 2704 2704 Size: 5845 AflII C'TTAA_G Cuts at: 820 1017 2698 820 Size: 197 1681 3967 Fragments arranged by size: 3967 1681 197 AflIII A'CryG_T Cuts at: 1203 2109 4744 5721 1203 Size: 906 2635 977 1327 Fragments arranged by size: 2635 1327 977 906 AgeI A'CCGG_T Cuts at: 4676 4676 Size: 5845 AhdI GACnn_n'nnGTC Cuts at: 4574 4574 Size: 5845 AleI CACnn'nnGTG Cuts at: 1166 2051 1166 Size: 885 4960 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5845 AlwNI CAG_nnn'CTG Cuts at: 2218 2873 3588 5160 2218 Size: 655 715 1572 2903 Fragments arranged by size: 2903 1572 715 655 ApaLI G'TGCA_C Cuts at: 2161 3092 3902 5058 2161 Size: 931 810 1156 2948 Fragments arranged by size: 2948 1156 931 810 ApoI r'AATT_y Cuts at: 1493 3154 3380 3532 5641 1493 Size: 1661 226 152 2109 1697 Fragments arranged by size: 2109 1697 1661 226 152 AseI AT'TA_AT Cuts at: 160 1032 4399 160 Size: 872 3367 1606 Fragments arranged by size: 3367 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 AvaI C'yCGr_G Cuts at: 1870 1943 3170 1870 Size: 73 1227 4545 Fragments arranged by size: 4545 1227 73 AvrII C'CTAG_G Cuts at: 2332 2332 Size: 5845 BamHI G'GATC_C Cuts at: 3175 3542 3175 Size: 367 5478 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2173 3166 4621 618 Size: 325 217 627 386 993 1455 1842 Fragments arranged by size: 1842 1455 993 627 386 325 217 BanII G_rGCy'C Cuts at: 1391 3164 1391 Size: 1773 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2553 3005 928 Size: 971 654 452 3768 Fragments arranged by size: 3768 971 654 452 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4036 4070 1903 Size: 34 2099 34 3678 Fragments arranged by size: 3678 2099 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3737 4947 3737 Size: 1210 4635 BclI T'GATC_A Cuts at: 1548 1548 Size: 5845 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2072 2714 4456 Size: 107 122 71 631 605 400 642 1742 Cuts at: 4456 136 Size: 1525 Fragments arranged by size: 1742 1525 642 631 605 400 122 107 71 BglII A'GATC_T Cuts at: 5840 5840 Size: 5845 BlpI GC'TnA_GC Cuts at: 3262 3262 Size: 5845 Bme1580I G_kGCm'C Cuts at: 1790 2012 2165 3096 3906 5062 1790 Size: 222 153 931 810 1156 2573 Fragments arranged by size: 2573 1156 931 810 222 153 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5845 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2045 3576 4534 Size: 416 53 83 399 24 628 1531 958 Cuts at: 4534 442 Size: 1753 Fragments arranged by size: 1753 1531 958 628 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5845 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2468 3067 4505 1239 Size: 1229 599 1438 2579 Fragments arranged by size: 2579 1438 1229 599 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3970 4835 5133 3970 Size: 865 298 4682 Fragments arranged by size: 4682 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2549 4508 882 Size: 617 1050 1959 2219 Fragments arranged by size: 2219 1959 1050 617 BsaAI yAC'GTr Cuts at: 493 1204 2929 3636 493 Size: 711 1725 707 2702 Fragments arranged by size: 2702 1725 711 707 BsaBI GATnn'nnATC Cuts at: 5839 5839 Size: 5845 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3006 3652 4034 4669 Size: 53 83 186 936 1473 646 382 635 Cuts at: 4669 275 Size: 1451 Fragments arranged by size: 1473 1451 936 646 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2664 4278 4676 4950 5097 5508 2664 Size: 1614 398 274 147 411 3001 Fragments arranged by size: 3001 1614 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3032 3062 3065 3095 1178 Size: 30 165 30 1629 30 3 30 3928 Fragments arranged by size: 3928 1629 165 30 30 30 30 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2780 2838 3053 3149 1186 Size: 1594 58 215 96 3882 Fragments arranged by size: 3882 1594 215 96 58 BseYI C'CCAG_C Cuts at: 2167 5048 5544 2167 Size: 2881 496 2468 Fragments arranged by size: 2881 2468 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5272 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2422 3295 4056 5084 1540 Size: 275 186 421 873 761 1028 2301 Fragments arranged by size: 2301 1028 873 761 421 275 186 BsiHKAI G_wGCw'C Cuts at: 2165 3096 3906 3991 5062 2165 Size: 931 810 85 1071 2948 Fragments arranged by size: 2948 1071 931 810 85 BsmI GAATG_Cn' Cuts at: 3356 3449 3356 Size: 93 5752 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2165 3096 3164 3906 3991 5062 Size: 399 222 153 931 68 742 85 1071 Cuts at: 5062 1391 Size: 2174 Fragments arranged by size: 2174 1071 931 742 399 222 153 85 68 BspEI T'CCGG_A Cuts at: 5508 5508 Size: 5845 BspHI T'CATG_A Cuts at: 3734 5464 3734 Size: 1730 4115 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2108 2203 2253 2567 3200 844 Size: 1264 95 50 314 633 3489 Fragments arranged by size: 3489 1264 633 314 95 50 BsrBI CCG'CTC Cuts at: 1394 1823 3732 1394 Size: 429 1909 3507 Fragments arranged by size: 3507 1909 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4340 4514 65 Size: 1183 3092 174 1396 Fragments arranged by size: 3092 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2176 4489 4676 1700 Size: 110 366 2313 187 2869 Fragments arranged by size: 2869 2313 366 187 110 BsrGI T'GTAC_A Cuts at: 96 2148 2274 2862 96 Size: 2052 126 588 3079 Fragments arranged by size: 3079 2052 588 126 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5845 BssSI C'ACGA_G Cuts at: 3905 4917 3905 Size: 1012 4833 BstAPI GCAn_nnn'nTGC Cuts at: 794 2218 2415 794 Size: 1424 197 4224 Fragments arranged by size: 4224 1424 197 BstBI TT'CG_AA Cuts at: 2118 4649 2118 Size: 2531 3314 BstEII G'GTnAC_C Cuts at: 1184 2353 3105 1184 Size: 1169 752 3924 Fragments arranged by size: 3924 1169 752 BstXI CCAn_nnnn'nTGG Cuts at: 2743 2743 Size: 5845 BstYI r'GATC_y Cuts at: 1972 3175 3542 3927 3944 5396 5482 5494 5840 Size: 1203 367 385 17 1452 86 12 346 Cuts at: 5840 1972 Size: 1977 Fragments arranged by size: 1977 1452 1203 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2069 2433 2895 2937 3054 513 Size: 552 1004 364 462 42 117 3304 Fragments arranged by size: 3304 1004 552 462 364 117 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2632 2722 487 Size: 683 56 1406 90 3610 Fragments arranged by size: 3610 1406 683 90 56 BtsI GCAGTG_nn' Cuts at: 2061 3361 4155 4175 2061 Size: 1300 794 20 3731 Fragments arranged by size: 3731 1300 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5845 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5810 DraI TTT'AAA Cuts at: 3149 3149 Size: 5845 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4852 809 Size: 507 3536 1802 Fragments arranged by size: 3536 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2178 2235 2430 Size: 54 1425 50 275 18 348 57 195 Cuts at: 2430 2733 2934 3292 4181 5810 8 Size: 303 201 358 889 1629 43 Fragments arranged by size: 1629 1425 889 358 348 303 275 201 195 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3292 1537 Size: 275 1480 4090 Fragments arranged by size: 4090 1480 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2115 2402 3775 1353 Size: 762 287 1373 3423 Fragments arranged by size: 3423 1373 762 287 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2080 2482 4432 4806 4952 387 Size: 1064 138 491 402 1950 374 146 1280 Fragments arranged by size: 1950 1280 1064 491 402 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2468 3067 3908 4505 5292 5560 1239 Size: 393 836 599 841 597 787 268 1524 Fragments arranged by size: 1524 841 836 787 599 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3082 3082 Size: 5845 EcoRI G'AATT_C Cuts at: 3154 3532 3154 Size: 378 5467 FspI TGC'GCA Cuts at: 4351 4351 Size: 5845 HaeII r_GCGC'y Cuts at: 1678 2207 2706 4992 1678 Size: 529 499 2286 2531 Fragments arranged by size: 2531 2286 529 499 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3032 3064 4486 4518 4560 Size: 32 135 32 1460 32 1422 32 42 Cuts at: 4560 4592 1373 Size: 32 2626 Fragments arranged by size: 2626 1460 1422 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3189 3435 669 Size: 988 281 1251 246 3079 Fragments arranged by size: 3079 1251 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5845 HpaI GTT'AAC Cuts at: 3435 3435 Size: 5845 KpnI G_GTAC'C Cuts at: 3170 3170 Size: 5845 MfeI C'AATT_G Cuts at: 3444 3444 Size: 5845 MluI A'CGCG_T Cuts at: 5721 5721 Size: 5845 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4959 5143 1305 Size: 3654 184 2007 Fragments arranged by size: 3654 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2936 10 Size: 54 1425 343 1104 2919 Fragments arranged by size: 2919 1425 1104 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2051 2741 3803 4162 4321 518 Size: 648 885 690 1062 359 159 2042 Fragments arranged by size: 2042 1062 885 690 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2325 2804 3261 3938 5086 5331 1606 Size: 719 479 457 677 1148 245 2120 Fragments arranged by size: 2120 1148 719 677 479 457 245 NaeI GCC'GGC Cuts at: 2178 2178 Size: 5845 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5293 NdeI CA'TA_TG Cuts at: 387 387 Size: 5845 NgoMIV G'CCGG_C Cuts at: 2176 2176 Size: 5845 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5845 NotI GC'GGCC_GC Cuts at: 3292 3292 Size: 5845 NspI r_CATG'y Cuts at: 1132 1229 2572 3203 4748 1132 Size: 97 1343 631 1545 2229 Fragments arranged by size: 2229 1545 1343 631 97 PasI CC'CwG_GG Cuts at: 1348 2080 1348 Size: 732 5113 PciI A'CATG_T Cuts at: 4744 4744 Size: 5845 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5845 PmeI GTTT'AAAC Cuts at: 3149 3149 Size: 5845 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3914 3946 5427 5459 3914 Size: 32 1481 32 4300 Fragments arranged by size: 4300 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3005 1613 Size: 1392 4453 PsiI TTA'TAA Cuts at: 3415 3415 Size: 5845 PstI C_TGCA'G Cuts at: 830 2214 2223 2577 3048 3197 830 Size: 1384 9 354 471 149 3478 Fragments arranged by size: 3478 1384 471 354 149 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5845 SalI G'TCGA_C Cuts at: 1936 3187 1936 Size: 1251 4594 SbfI CC_TGCA'GG Cuts at: 3197 3197 Size: 5845 ScaI AGT'ACT Cuts at: 4093 5805 4093 Size: 1712 4133 SexAI A'CCwGG_T Cuts at: 2781 2781 Size: 5845 SfcI C'TryA_G Cuts at: 826 1045 2210 2219 2573 2818 3044 3193 4328 Size: 219 1165 9 354 245 226 149 1135 Cuts at: 4328 5009 5200 826 Size: 681 191 1471 Fragments arranged by size: 1471 1165 1135 681 354 245 226 219 191 149 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5845 SmaI CCC'GGG Cuts at: 1872 3172 1872 Size: 1300 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2698 3949 4850 5112 820 Size: 197 926 755 1251 901 262 1553 Fragments arranged by size: 1553 1251 926 901 755 262 197 SnaBI TAC'GTA Cuts at: 493 3636 493 Size: 3143 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5845 SphI G_CATG'C Cuts at: 1132 3203 1132 Size: 2071 3774 SspI AAT'ATT Cuts at: 5 52 3769 5 Size: 47 3717 2081 Fragments arranged by size: 3717 2081 47 StuI AGG'CCT Cuts at: 1517 2337 1517 Size: 820 5025 StyI C'CwwG_G Cuts at: 513 1065 2332 2718 3284 513 Size: 552 1267 386 566 3074 Fragments arranged by size: 3074 1267 566 552 386 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3035 3111 3872 3889 4042 4227 4672 Size: 1255 1455 76 761 17 153 185 445 Cuts at: 4672 325 Size: 1498 Fragments arranged by size: 1498 1455 1255 761 445 185 153 76 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2148 2274 2862 Size: 274 80 33 51 1443 171 126 588 Cuts at: 2862 4091 5803 96 Size: 1229 1712 138 Fragments arranged by size: 1712 1443 1229 588 274 171 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5805 5837 5805 Size: 32 5813 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5845 XbaI T'CTAG_A Cuts at: 3181 3181 Size: 5845 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2845 2845 Size: 5845 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5845 XmaI C'CCGG_G Cuts at: 1870 3170 1870 Size: 1300 4545 XmnI GAAnn'nnTTC Cuts at: 3974 3974 Size: 5845 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3007 3653 276 Size: 53 83 186 936 1473 646 2468 Fragments arranged by size: 2468 1473 936 646 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI