GPR157 ORF clone pFN21AE9534

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Halotag ORF Clone pFN21AE9534

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Clone info.

Species

human

Clone Name

pFN21AE9534 Sequence (with vector) Restriction Map

Product ID

FHC11100

ORF Sequence

DNA sequence (1005 bp)
Protein sequence (335 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9534

Symbol

GPR157

Description

G protein-coupled receptor 157

Original Clone ID

cp99534

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1005 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 335 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS100.2 4e-114 100.0 GPR157 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR022343 25 43 PR02001 GCR1-cAMP receptor IPR022343 44 59 PR02001 GCR1-cAMP receptor IPR022343 79 97 PR02001 GCR1-cAMP receptor IPR022343 230 248 PR02001 GCR1-cAMP receptor HMMPfam IPR000832 18 272 PF00002 GPCR ProfileScan IPR017981 14 283 PS50261 GPCR IPR017452 28 192 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 15 RAVVLLSCALSALGSGLLVATHA 37 SECONDARY 23 2 49 LLLFLSLADLLSAASYFYGVLQN 71 SECONDARY 23 3 93 SFFWTVAIALYLYLSIVRAARG 114 PRIMARY 22 4 121 LWAFHVVSWGVPLVITVAAVAL 142 PRIMARY 22 5 171 LLTGKLWEMLAYVLLPLLYLLV 192 PRIMARY 22 6 227 LVLIPLIFIGLRVWSTVRFVLTL 249 PRIMARY 23 7 277 IMFVLCTRAVRTRLFSLCCCCC 298 SECONDARY 22 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9534 Download

>KIEE9534  1005 bp
ATGCAGCCGTCCCCGCCGCCCACCGAGCTGGTGCCGTCGGAGCGCGCCGTGGTGCTGCTG
TCGTGCGCACTCTCCGCGCTCGGCTCGGGCCTGCTGGTGGCCACGCACGCCCTGTGGCCC
GACCTGCGCAGCCGGGCACGGCGCCTGCTGCTCTTCCTGTCGCTGGCCGACCTGCTCTCG
GCCGCCTCCTACTTCTACGGAGTGCTGCAGAACTTCGCGGGCCCGTCGTGGGACTGCGTG
CTGCAGGGCGCGCTGTCCACCTTCGCCAACACCAGCTCCTTCTTCTGGACCGTGGCCATT
GCGCTCTACTTGTACCTCAGCATCGTCCGCGCCGCGCGCGGGCCTCGCACAGATCGCCTG
CTTTGGGCCTTCCATGTCGTCAGCTGGGGGGTCCCGTTGGTCATCACTGTGGCAGCCGTC
GCCCTGAAGAAGATTGGCTATGACGCCTCGGACGTGTCTGTGGGCTGGTGCTGGATCGAC
CTGGAGGCCAAGGACCATGTCCTGTGGATGCTGCTGACGGGGAAGCTGTGGGAGATGCTG
GCATATGTGCTGCTGCCTCTGCTGTACCTCCTGGTCCGGAAGCACATCAACAGAGCGCAC
ACGGCACTCTCTGAGTACCGGCCCATCCTCTCCCAGGAGCACCGCCTGCTGCGCCACTCC
TCCATGGCGGACAAGAAGCTGGTGCTCATCCCGCTCATCTTCATCGGCCTCAGGGTCTGG
AGCACCGTGCGGTTCGTGCTGACCCTCTGTGGCTCCCCGGCCGTGCAGACGCCGGTGCTG
GTGGTTCTGCATGGTATCGGGAACACGTTTCAGGGAGGTGCCAACTGCATCATGTTCGTC
CTATGCACCCGCGCCGTCCGAACTCGGCTCTTCTCTCTCTGTTGCTGCTGCTGCTCTTCT
CAGCCTCCCACCAAGAGCCCGGCTGGCACTCCCAAGGCTCCCGCGCCTTCCAAGCCAGGA
GAATCTCAGGAATCCCAAGGGACCCCAGGGGAACTTCCAAGCACC

ORF Protein

Cloned ORF protein sequence for pFN21AE9534 Download

>KIEE9534  335 aa
MQPSPPPTELVPSERAVVLLSCALSALGSGLLVATHALWPDLRSRARRLLLFLSLADLLS
AASYFYGVLQNFAGPSWDCVLQGALSTFANTSSFFWTVAIALYLYLSIVRAARGPRTDRL
LWAFHVVSWGVPLVITVAAVALKKIGYDASDVSVGWCWIDLEAKDHVLWMLLTGKLWEML
AYVLLPLLYLLVRKHINRAHTALSEYRPILSQEHRLLRHSSMADKKLVLIPLIFIGLRVW
STVRFVLTLCGSPAVQTPVLVVLHGIGNTFQGGANCIMFVLCTRAVRTRLFSLCCCCCSS
QPPTKSPAGTPKAPAPSKPGESQESQGTPGELPST

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9534 Download

>pFN21AE9534  5710 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCAGCCGTCCCCGCCGCCCACCGAGCTGGTGCC
GTCGGAGCGCGCCGTGGTGCTGCTGTCGTGCGCACTCTCCGCGCTCGGCTCGGGCCTGCT
GGTGGCCACGCACGCCCTGTGGCCCGACCTGCGCAGCCGGGCACGGCGCCTGCTGCTCTT
CCTGTCGCTGGCCGACCTGCTCTCGGCCGCCTCCTACTTCTACGGAGTGCTGCAGAACTT
CGCGGGCCCGTCGTGGGACTGCGTGCTGCAGGGCGCGCTGTCCACCTTCGCCAACACCAG
CTCCTTCTTCTGGACCGTGGCCATTGCGCTCTACTTGTACCTCAGCATCGTCCGCGCCGC
GCGCGGGCCTCGCACAGATCGCCTGCTTTGGGCCTTCCATGTCGTCAGCTGGGGGGTCCC
GTTGGTCATCACTGTGGCAGCCGTCGCCCTGAAGAAGATTGGCTATGACGCCTCGGACGT
GTCTGTGGGCTGGTGCTGGATCGACCTGGAGGCCAAGGACCATGTCCTGTGGATGCTGCT
GACGGGGAAGCTGTGGGAGATGCTGGCATATGTGCTGCTGCCTCTGCTGTACCTCCTGGT
CCGGAAGCACATCAACAGAGCGCACACGGCACTCTCTGAGTACCGGCCCATCCTCTCCCA
GGAGCACCGCCTGCTGCGCCACTCCTCCATGGCGGACAAGAAGCTGGTGCTCATCCCGCT
CATCTTCATCGGCCTCAGGGTCTGGAGCACCGTGCGGTTCGTGCTGACCCTCTGTGGCTC
CCCGGCCGTGCAGACGCCGGTGCTGGTGGTTCTGCATGGTATCGGGAACACGTTTCAGGG
AGGTGCCAACTGCATCATGTTCGTCCTATGCACCCGCGCCGTCCGAACTCGGCTCTTCTC
TCTCTGTTGCTGCTGCTGCTCTTCTCAGCCTCCCACCAAGAGCCCGGCTGGCACTCCCAA
GGCTCCCGCGCCTTCCAAGCCAGGAGAATCTCAGGAATCCCAAGGGACCCCAGGGGAACT
TCCAAGCACCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT