(Circular) (Six-base) MAPSORT of: pFN21AE9534.seq Check: 1127 from: 1 to: 5710 pFN21AE9534 5710 bp With 182 enzymes: SgfI * March 28, 2012 12:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3520 278 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3053 1937 Size: 1116 4594 Acc65I G'GTAC_C Cuts at: 3031 3031 Size: 5710 AclI AA'CG_TT Cuts at: 1563 3837 4210 1563 Size: 2274 373 3063 Fragments arranged by size: 3063 2274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2450 3773 5157 5425 1632 Size: 818 1323 1384 268 1917 Fragments arranged by size: 1917 1384 1323 818 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5513 AflIII A'CryG_T Cuts at: 1203 2457 2808 4609 5586 1203 Size: 1254 351 1801 977 1327 Fragments arranged by size: 1801 1327 1254 977 351 AgeI A'CCGG_T Cuts at: 4541 4541 Size: 5710 AhdI GACnn_n'nnGTC Cuts at: 4439 4439 Size: 5710 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5710 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5710 AlwNI CAG_nnn'CTG Cuts at: 2721 3453 5025 2721 Size: 732 1572 3406 Fragments arranged by size: 3406 1572 732 ApaI G_GGCC'C Cuts at: 2228 2228 Size: 5710 ApaLI G'TGCA_C Cuts at: 3767 4923 3767 Size: 1156 4554 ApoI r'AATT_y Cuts at: 1493 3019 3245 3397 5506 1493 Size: 1526 226 152 2109 1697 Fragments arranged by size: 2109 1697 1526 226 152 AseI AT'TA_AT Cuts at: 160 1032 4264 160 Size: 872 3232 1606 Fragments arranged by size: 3232 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 AvaI C'yCGr_G Cuts at: 1870 1943 2089 3035 1870 Size: 73 146 946 4545 Fragments arranged by size: 4545 946 146 73 BamHI G'GATC_C Cuts at: 3040 3407 3040 Size: 367 5343 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 2145 2822 3031 4486 Size: 325 217 627 248 110 677 209 1455 Cuts at: 4486 618 Size: 1842 Fragments arranged by size: 1842 1455 677 627 325 248 217 209 110 BanII G_rGCy'C Cuts at: 1391 2228 2924 3029 1391 Size: 837 696 105 4072 Fragments arranged by size: 4072 837 696 105 BbeI G_GCGC'C Cuts at: 2149 2149 Size: 5710 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4739 BbvCI CC'TCA_GC Cuts at: 2321 2321 Size: 5710 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2821 2855 3901 3935 1903 Size: 34 50 34 800 34 1046 34 3678 Fragments arranged by size: 3678 1046 800 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3602 4812 3602 Size: 1210 4500 BclI T'GATC_A Cuts at: 1548 1548 Size: 5710 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2928 4321 136 Size: 107 122 71 631 605 1256 1393 1525 Fragments arranged by size: 1525 1393 1256 631 605 122 107 71 BglII A'GATC_T Cuts at: 5705 5705 Size: 5710 BlpI GC'TnA_GC Cuts at: 3127 3127 Size: 5710 Bme1580I G_kGCm'C Cuts at: 1790 2143 2228 3771 4927 1790 Size: 353 85 1543 1156 2573 Fragments arranged by size: 2573 1543 1156 353 85 BmgBI CAC'GTC Cuts at: 1318 2458 1318 Size: 1140 4570 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3441 4399 442 Size: 416 53 83 399 24 2024 958 1753 Fragments arranged by size: 2024 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2507 2743 4370 1239 Size: 1268 236 1627 2579 Fragments arranged by size: 2579 1627 1268 236 Bpu10I CC'TnA_GC Cuts at: 2321 2321 Size: 5710 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3835 4700 4998 3835 Size: 865 298 4547 Fragments arranged by size: 4547 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4373 882 Size: 617 2874 2219 Fragments arranged by size: 2874 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3501 493 Size: 711 2297 2702 Fragments arranged by size: 2702 2297 711 BsaBI GATnn'nnATC Cuts at: 5704 5704 Size: 5710 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2146 2448 2774 3517 Size: 53 83 186 936 613 302 326 743 Cuts at: 3517 3899 4534 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 743 635 613 382 326 302 186 83 53 BsaWI w'CCGG_w Cuts at: 2580 4143 4541 4815 4962 5373 2580 Size: 1563 398 274 147 411 2917 Fragments arranged by size: 2917 1563 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2264 2294 1178 Size: 30 165 30 861 30 4594 Fragments arranged by size: 4594 861 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2653 1186 Size: 1467 4243 BseYI C'CCAG_C Cuts at: 2388 4913 5409 2388 Size: 2525 496 2689 Fragments arranged by size: 2689 2525 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2789 1163 Size: 573 1053 4084 Fragments arranged by size: 4084 1053 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2187 2766 3160 3921 4949 1540 Size: 275 186 186 579 394 761 1028 2301 Fragments arranged by size: 2301 1028 761 579 394 275 186 186 BsiHKAI G_wGCw'C Cuts at: 2646 2691 2729 3771 3856 4927 2646 Size: 45 38 1042 85 1071 3429 Fragments arranged by size: 3429 1071 1042 85 45 38 BsmI GAATG_Cn' Cuts at: 3221 3314 3221 Size: 93 5617 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2143 2228 2646 2691 2729 2924 3029 Size: 399 353 85 418 45 38 195 105 Cuts at: 3029 3771 3856 4927 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 418 399 353 195 105 85 85 45 38 BspEI T'CCGG_A Cuts at: 2580 5373 2580 Size: 2793 2917 BspHI T'CATG_A Cuts at: 3599 5329 3599 Size: 1730 3980 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2136 2184 3065 844 Size: 1292 48 881 3489 Fragments arranged by size: 3489 1292 881 48 BsrBI CCG'CTC Cuts at: 1394 1823 2698 3597 1394 Size: 429 875 899 3507 Fragments arranged by size: 3507 899 875 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2301 4205 4379 65 Size: 1183 1053 1904 174 1396 Fragments arranged by size: 1904 1396 1183 1053 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2622 2776 4354 4541 1700 Size: 110 812 154 1578 187 2869 Fragments arranged by size: 2869 1578 812 187 154 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5710 BssHII G'CGCG_C Cuts at: 1925 2047 2253 2339 1925 Size: 122 206 86 5296 Fragments arranged by size: 5296 206 122 86 BssSI C'ACGA_G Cuts at: 3770 4782 3770 Size: 1012 4698 BstAPI GCAn_nnn'nTGC Cuts at: 794 2650 794 Size: 1856 3854 BstBI TT'CG_AA Cuts at: 4514 4514 Size: 5710 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5710 BstXI CCAn_nnnn'nTGG Cuts at: 2032 2032 Size: 5710 BstYI r'GATC_y Cuts at: 1972 3040 3407 3792 3809 5261 5347 5359 5705 Size: 1068 367 385 17 1452 86 12 346 Cuts at: 5705 1972 Size: 1977 Fragments arranged by size: 1977 1452 1068 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2714 2714 Size: 5710 BtgI C'CryG_G Cuts at: 513 1065 2052 2295 2667 513 Size: 552 987 243 372 3556 Fragments arranged by size: 3556 987 552 372 243 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4971 Fragments arranged by size: 4971 683 56 BtsI GCAGTG_nn' Cuts at: 3226 4020 4040 3226 Size: 794 20 4896 Fragments arranged by size: 4896 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5710 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5675 DraI TTT'AAA Cuts at: 3014 3014 Size: 5710 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4717 809 Size: 507 3401 1802 Fragments arranged by size: 3401 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2103 2169 2184 Size: 54 1425 50 275 18 273 66 15 Cuts at: 2184 2298 2763 3157 4046 5675 8 Size: 114 465 394 889 1629 43 Fragments arranged by size: 1629 1425 889 465 394 275 273 114 66 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2184 2763 3157 1537 Size: 275 372 579 394 4090 Fragments arranged by size: 4090 579 394 372 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2162 2879 2905 3640 1353 Size: 809 717 26 735 3423 Fragments arranged by size: 3423 809 735 717 26 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2687 4297 4671 4817 387 Size: 1064 138 1098 1610 374 146 1280 Fragments arranged by size: 1610 1280 1098 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2450 2507 2743 3773 4370 5157 5425 Size: 393 818 57 236 1030 597 787 268 Cuts at: 5425 1239 Size: 1524 Fragments arranged by size: 1524 1030 818 787 597 393 268 236 57 EcoNI CCTnn'n_nnAGG Cuts at: 2636 2636 Size: 5710 EcoO109I rG'GnC_Cy Cuts at: 2395 2985 2395 Size: 590 5120 EcoRI G'AATT_C Cuts at: 3019 3397 3019 Size: 378 5332 FspI TGC'GCA Cuts at: 2071 2132 4216 2071 Size: 61 2084 3565 Fragments arranged by size: 3565 2084 61 FspAI rTGC'GCAy Cuts at: 2071 2071 Size: 5710 HaeII r_GCGC'y Cuts at: 1678 2149 4857 1678 Size: 471 2708 2531 Fragments arranged by size: 2708 2531 471 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2165 2197 4351 4383 4425 Size: 32 135 32 593 32 2154 32 42 Cuts at: 4425 4457 1373 Size: 32 2626 Fragments arranged by size: 2626 2154 593 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3054 3300 669 Size: 988 281 1116 246 3079 Fragments arranged by size: 3079 1116 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5710 HpaI GTT'AAC Cuts at: 3300 3300 Size: 5710 KasI G'GCGC_C Cuts at: 2145 2145 Size: 5710 KpnI G_GTAC'C Cuts at: 3035 3035 Size: 5710 MfeI C'AATT_G Cuts at: 3309 3309 Size: 5710 MluI A'CGCG_T Cuts at: 5586 5586 Size: 5710 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2022 4824 5008 1305 Size: 717 2802 184 2007 Fragments arranged by size: 2802 2007 717 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2105 2300 10 Size: 54 1425 343 273 195 3420 Fragments arranged by size: 3420 1425 343 273 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 2411 3668 4027 4186 518 Size: 648 1245 1257 359 159 2042 Fragments arranged by size: 2042 1257 1245 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2388 3126 3803 4951 5196 1606 Size: 782 738 677 1148 245 2120 Fragments arranged by size: 2120 1148 782 738 677 245 NarI GG'CG_CC Cuts at: 2146 2146 Size: 5710 NcoI C'CATG_G Cuts at: 513 1065 2667 513 Size: 552 1602 3556 Fragments arranged by size: 3556 1602 552 NdeI CA'TA_TG Cuts at: 387 2548 387 Size: 2161 3549 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5710 NotI GC'GGCC_GC Cuts at: 3157 3157 Size: 5710 NspI r_CATG'y Cuts at: 1132 1229 3068 4613 1132 Size: 97 1839 1545 2229 Fragments arranged by size: 2229 1839 1545 97 PasI CC'CwG_GG Cuts at: 1348 2990 1348 Size: 1642 4068 PciI A'CATG_T Cuts at: 4609 4609 Size: 5710 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5710 PmeI GTTT'AAAC Cuts at: 3014 3014 Size: 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3779 3811 5292 5324 3779 Size: 32 1481 32 4165 Fragments arranged by size: 4165 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2395 2985 2395 Size: 590 5120 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5710 PsiI TTA'TAA Cuts at: 3280 3280 Size: 5710 PspOMI G'GGCC_C Cuts at: 2224 2224 Size: 5710 PstI C_TGCA'G Cuts at: 830 2214 2250 3062 830 Size: 1384 36 812 3478 Fragments arranged by size: 3478 1384 812 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5710 PvuII CAG'CTG Cuts at: 2388 2388 Size: 5710 SalI G'TCGA_C Cuts at: 1936 3052 1936 Size: 1116 4594 SanDI GG'GwC_CC Cuts at: 2395 2985 2395 Size: 590 5120 SapI GCTCTTCn'nnn_ Cuts at: 2162 2879 2905 2162 Size: 717 26 4967 Fragments arranged by size: 4967 717 26 SbfI CC_TGCA'GG Cuts at: 3062 3062 Size: 5710 ScaI AGT'ACT Cuts at: 3958 5670 3958 Size: 1712 3998 SfcI C'TryA_G Cuts at: 826 1045 2210 2246 3058 4193 4874 5065 826 Size: 219 1165 36 812 1135 681 191 1471 Fragments arranged by size: 1471 1165 1135 812 681 219 191 36 SfoI GGC'GCC Cuts at: 2147 2147 Size: 5710 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 SgrAI Cr'CCGG_yG Cuts at: 1700 2776 1700 Size: 1076 4634 SmaI CCC'GGG Cuts at: 1872 3037 1872 Size: 1165 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3814 4715 4977 820 Size: 197 926 1871 901 262 1553 Fragments arranged by size: 1871 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3501 493 Size: 3008 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5710 SphI G_CATG'C Cuts at: 1132 3068 1132 Size: 1936 3774 SspI AAT'ATT Cuts at: 5 52 3634 5 Size: 47 3582 2081 Fragments arranged by size: 3582 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5710 StyI C'CwwG_G Cuts at: 513 1065 2493 2667 2937 2980 3149 513 Size: 552 1428 174 270 43 169 3074 Fragments arranged by size: 3074 1428 552 270 174 169 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3737 3754 3907 4092 4537 325 Size: 1255 2157 17 153 185 445 1498 Fragments arranged by size: 2157 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3956 5668 96 Size: 274 80 33 51 1443 1979 1712 138 Fragments arranged by size: 1979 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5670 5702 5670 Size: 32 5678 Tth111I GACn'n_nGTC Cuts at: 1316 2501 1316 Size: 1185 4525 XbaI T'CTAG_A Cuts at: 3046 3046 Size: 5710 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5710 XmaI C'CCGG_G Cuts at: 1870 3035 1870 Size: 1165 4545 XmnI GAAnn'nnTTC Cuts at: 3839 3839 Size: 5710 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3518 276 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI SrfI SwaI XcmI