Restriction map for
pFN21AE9534
(Linear) (Six-base) MAPSORT of: KIEE9534.seq Check: 9414 from: 1 to: 1005
KIEE9534.orf
With 182 enzymes: SgfI *
March 22, 2012 18:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 1005
Size: 445 560
AflIII A'CryG_T
Cuts at: 0 452 803 1005
Size: 452 351 202
Fragments arranged by size:
452 351 202
AlwNI CAG_nnn'CTG
Cuts at: 0 716 1005
Size: 716 289
ApaI G_GGCC'C
Cuts at: 0 223 1005
Size: 223 782
AvaI C'yCGr_G
Cuts at: 0 84 1005
Size: 84 921
BanI G'GyrC_C
Cuts at: 0 30 140 817 1005
Size: 30 110 677 188
Fragments arranged by size:
677 188 110 30
BanII G_rGCy'C
Cuts at: 0 223 919 1005
Size: 223 696 86
Fragments arranged by size:
696 223 86
BbeI G_GCGC'C
Cuts at: 0 144 1005
Size: 144 861
BbvCI CC'TCA_GC
Cuts at: 0 316 1005
Size: 316 689
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 816 850 1005
Size: 816 34 155
Fragments arranged by size:
816 155 34
BglI GCCn_nnn'nGGC
Cuts at: 0 923 1005
Size: 923 82
Bme1580I G_kGCm'C
Cuts at: 0 138 223 1005
Size: 138 85 782
Fragments arranged by size:
782 138 85
BmgBI CAC'GTC
Cuts at: 0 453 1005
Size: 453 552
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 738 1005
Size: 502 236 267
Fragments arranged by size:
502 267 236
Bpu10I CC'TnA_GC
Cuts at: 0 316 1005
Size: 316 689
BsaHI Gr'CG_yC
Cuts at: 0 141 443 769 1005
Size: 141 302 326 236
Fragments arranged by size:
326 302 236 141
BsaWI w'CCGG_w
Cuts at: 0 575 1005
Size: 575 430
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 259 289 1005
Size: 259 30 716
Fragments arranged by size:
716 259 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 648 1005
Size: 648 357
BseYI C'CCAG_C
Cuts at: 0 383 1005
Size: 383 622
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 1005
Size: 784 221
BsiEI CG_ry'CG
Cuts at: 0 182 761 1005
Size: 182 579 244
Fragments arranged by size:
579 244 182
BsiHKAI G_wGCw'C
Cuts at: 0 641 686 724 1005
Size: 641 45 38 281
Fragments arranged by size:
641 281 45 38
Bsp1286I G_dGCh'C
Cuts at: 0 138 223 641 686 724 919 1005
Size: 138 85 418 45 38 195 86
Fragments arranged by size:
418 195 138 86 85 45 38
BspEI T'CCGG_A
Cuts at: 0 575 1005
Size: 575 430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 131 179 1005
Size: 131 48 826
Fragments arranged by size:
826 131 48
BsrBI CCG'CTC
Cuts at: 0 693 1005
Size: 693 312
BsrDI GCAATG_nn'
Cuts at: 0 296 1005
Size: 296 709
BsrFI r'CCGG_y
Cuts at: 0 617 771 1005
Size: 617 154 234
Fragments arranged by size:
617 234 154
BssHII G'CGCG_C
Cuts at: 0 42 248 334 1005
Size: 42 206 86 671
Fragments arranged by size:
671 206 86 42
BstAPI GCAn_nnn'nTGC
Cuts at: 0 645 1005
Size: 645 360
BstXI CCAn_nnnn'nTGG
Cuts at: 0 27 1005
Size: 27 978
Bsu36I CC'TnA_GG
Cuts at: 0 709 1005
Size: 709 296
BtgI C'CryG_G
Cuts at: 0 47 290 662 1005
Size: 47 243 372 343
Fragments arranged by size:
372 343 243 47
EaeI y'GGCC_r
Cuts at: 0 98 164 179 293 758 1005
Size: 98 66 15 114 465 247
Fragments arranged by size:
465 247 114 98 66 15
EagI C'GGCC_G
Cuts at: 0 179 758 1005
Size: 179 579 247
Fragments arranged by size:
579 247 179
EarI CTCTTCn'nnn_
Cuts at: 0 157 874 900 1005
Size: 157 717 26 105
Fragments arranged by size:
717 157 105 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 682 1005
Size: 682 323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 502 738 1005
Size: 445 57 236 267
Fragments arranged by size:
445 267 236 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 631 1005
Size: 631 374
EcoO109I rG'GnC_Cy
Cuts at: 0 390 980 1005
Size: 390 590 25
Fragments arranged by size:
590 390 25
FspI TGC'GCA
Cuts at: 0 66 127 1005
Size: 66 61 878
Fragments arranged by size:
878 66 61
FspAI rTGC'GCAy
Cuts at: 0 66 1005
Size: 66 939
HaeII r_GCGC'y
Cuts at: 0 144 1005
Size: 144 861
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 160 192 1005
Size: 160 32 813
Fragments arranged by size:
813 160 32
KasI G'GCGC_C
Cuts at: 0 140 1005
Size: 140 865
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 1005
Size: 17 988
MscI TGG'CCA
Cuts at: 0 100 295 1005
Size: 100 195 710
Fragments arranged by size:
710 195 100
MslI CAynn'nnrTG
Cuts at: 0 406 1005
Size: 406 599
MspA1I CmG'CkG
Cuts at: 0 383 1005
Size: 383 622
NarI GG'CG_CC
Cuts at: 0 141 1005
Size: 141 864
NcoI C'CATG_G
Cuts at: 0 662 1005
Size: 662 343
NdeI CA'TA_TG
Cuts at: 0 543 1005
Size: 543 462
PasI CC'CwG_GG
Cuts at: 0 985 1005
Size: 985 20
PpuMI rG'GwC_Cy
Cuts at: 0 390 980 1005
Size: 390 590 25
Fragments arranged by size:
590 390 25
PspOMI G'GGCC_C
Cuts at: 0 219 1005
Size: 219 786
PstI C_TGCA'G
Cuts at: 0 209 245 1005
Size: 209 36 760
Fragments arranged by size:
760 209 36
PvuII CAG'CTG
Cuts at: 0 383 1005
Size: 383 622
SanDI GG'GwC_CC
Cuts at: 0 390 980 1005
Size: 390 590 25
Fragments arranged by size:
590 390 25
SapI GCTCTTCn'nnn_
Cuts at: 0 157 874 900 1005
Size: 157 717 26 105
Fragments arranged by size:
717 157 105 26
SfcI C'TryA_G
Cuts at: 0 205 241 1005
Size: 205 36 764
Fragments arranged by size:
764 205 36
SfoI GGC'GCC
Cuts at: 0 142 1005
Size: 142 863
SgrAI Cr'CCGG_yG
Cuts at: 0 771 1005
Size: 771 234
StyI C'CwwG_G
Cuts at: 0 488 662 932 975 1005
Size: 488 174 270 43 30
Fragments arranged by size:
488 270 174 43 30
Tth111I GACn'n_nGTC
Cuts at: 0 496 1005
Size: 496 509
Enzymes that do cut:
AcuI AflIII AlwNI ApaI AvaI BanI BanII BbeI
BbvCI BcgI BglI Bme1580I BmgBI BpmI Bpu10I BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstXI
Bsu36I BtgI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I FspI FspAI HaeII Hin4I KasI MmeI MscI
MslI MspA1I NarI NcoI NdeI PasI PpuMI PspOMI
PstI PvuII SanDI SapI SfcI SfoI SgrAI StyI
Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI
BglII BlpI BmrI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsiWI BsmI BsmBI BspHI BsrGI BssSI BstBI
BstEII BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EcoICRI EcoRI EcoRV FalI FseI HincII
HindIII HpaI KpnI MfeI MluI NaeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SbfI ScaI SexAI SfiI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI XbaI XcmI XhoI
XmaI XmnI ZraI