OPN5 ORF clone pFN21AE9449

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE9449

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Clone info.

Species

human

Clone Name

pFN21AE9449 Sequence (with vector) Restriction Map

Product ID

FHC31413

ORF Sequence

DNA sequence (1062 bp)
Protein sequence (354 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9449

Symbol

OPN5

Alias : GPR136, GRP136, PGR12, TMEM13

Description

opsin 5, transcript variant 1

Original Clone ID

cp99449

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1062 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 354 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4923.1 2.2e-106 100.0 OPN5 CCDS3687.1 2.4e-22 32.4 RRH CCDS7376.1 4.8e-20 30.8 OPN4 CCDS31237.1 1.4e-18 30.4 OPN4 CCDS31072.1 1.7e-17 30.4 OPN3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 35 59 PR00237 GPCR IPR000276 68 89 PR00237 GPCR IPR002962 78 93 PR01244 Peropsin IPR002962 109 125 PR01244 Peropsin IPR000276 113 135 PR00237 GPCR IPR000276 148 169 PR00237 GPCR IPR000276 200 223 PR00237 GPCR IPR000276 250 274 PR00237 GPCR IPR002962 274 292 PR01244 Peropsin IPR000276 288 314 PR00237 GPCR HMMPfam IPR000276 50 306 PF00001 GPCR ProfileScan IPR017452 50 306 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 29 SWEADLVAGFYLTIIGILSTFGN 51 PRIMARY 23 2 71 MTINLAVCDLGISVVGKPFTIIS 93 SECONDARY 23 3 111 WAGFFFGCGSLITMTAVSLDRYL 133 SECONDARY 23 4 150 YICLAAIWAYASFWTTMPLVGLG 172 SECONDARY 23 5 200 ILNILFFCLLLPTAVIVFSYVKI 222 PRIMARY 23 6 251 TKVAMLICAGFLIAWIPYAVVSV 273 PRIMARY 23 7 298 AAMYNPIIYQVIDYKFACCQTGG 320 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_859528 354 609042 100.0 100.0 XP_016865905 408 609042 100.0 99.4 XP_016865900 298 609042 100.0 84.2 XP_016865899 299 609042 82.1 83.6 NP_006574 337 605224 32.4 83.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9449 Download

>KIEE9449  1062 bp
ATGGCGTTAAATCACACTGCCCTGCCTCAGGACGAGCGCCTGCCCCATTACCTTCGAGAT
GGGGATCCTTTTGCTTCCAAACTTTCTTGGGAAGCGGATTTAGTGGCTGGCTTTTACCTA
ACAATAATTGGGATTCTGTCCACATTTGGAAATGGATATGTCCTTTACATGTCTTCTAGA
CGAAAGAAGAAGCTGAGACCCGCTGAAATAATGACTATCAATTTAGCAGTCTGTGATCTG
GGGATTTCAGTTGTAGGCAAGCCGTTCACCATCATCTCTTGCTTTTGTCACCGCTGGGTG
TTTGGCTGGATCGGCTGCCGCTGGTATGGATGGGCTGGATTTTTCTTTGGCTGTGGAAGC
CTTATCACCATGACTGCTGTCAGCCTGGATCGATATTTGAAAATCTGCTATTTATCTTAT
GGGGTTTGGCTGAAAAGAAAGCACGCCTACATCTGCCTGGCAGCCATCTGGGCCTATGCT
TCCTTCTGGACCACCATGCCCTTGGTAGGTCTGGGGGACTACGTACCTGAGCCCTTCGGA
ACCTCGTGCACCCTGGACTGGTGGCTGGCCCAGGCCTCGGTAGGGGGCCAGGTTTTCATC
CTGAACATCCTCTTCTTCTGCCTCTTGCTCCCAACGGCTGTGATCGTGTTCTCCTACGTA
AAGATCATTGCCAAGGTTAAGTCCTCTTCCAAAGAAGTAGCTCATTTCGACAGTCGGATC
CATAGCAGCCATGTGCTGGAAATGAAACTGACAAAGGTAGCGATGTTGATTTGTGCTGGA
TTCCTGATTGCCTGGATTCCTTATGCAGTGGTGTCTGTGTGGTCAGCTTTTGGAAGGCCA
GACTCCATTCCCATACAGCTCTCTGTGGTGCCAACCCTACTTGCAAAATCTGCAGCGATG
TACAATCCCATCATTTACCAAGTTATTGATTACAAATTTGCCTGTTGCCAAACTGGTGGT
TTGAAAGCAACCAAGAAGAAGTCTCTGGAAGGCTTCAGGCTGCACACCGTAACCACAGTC
AGGAAGTCTTCTGCTGTGCTGGAAATTCATGAAGAGTGGGAA

ORF Protein

Cloned ORF protein sequence for pFN21AE9449 Download

>KIEE9449  354 aa
MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTIIGILSTFGNGYVLYMSSR
RKKKLRPAEIMTINLAVCDLGISVVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGS
LITMTAVSLDRYLKICYLSYGVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYVPEPFG
TSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRI
HSSHVLEMKLTKVAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAM
YNPIIYQVIDYKFACCQTGGLKATKKKSLEGFRLHTVTTVRKSSAVLEIHEEWE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9449 Download

>pFN21AE9449  5767 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGTTAAATCACACTGCCCTGCCTCAGGACGA
GCGCCTGCCCCATTACCTTCGAGATGGGGATCCTTTTGCTTCCAAACTTTCTTGGGAAGC
GGATTTAGTGGCTGGCTTTTACCTAACAATAATTGGGATTCTGTCCACATTTGGAAATGG
ATATGTCCTTTACATGTCTTCTAGACGAAAGAAGAAGCTGAGACCCGCTGAAATAATGAC
TATCAATTTAGCAGTCTGTGATCTGGGGATTTCAGTTGTAGGCAAGCCGTTCACCATCAT
CTCTTGCTTTTGTCACCGCTGGGTGTTTGGCTGGATCGGCTGCCGCTGGTATGGATGGGC
TGGATTTTTCTTTGGCTGTGGAAGCCTTATCACCATGACTGCTGTCAGCCTGGATCGATA
TTTGAAAATCTGCTATTTATCTTATGGGGTTTGGCTGAAAAGAAAGCACGCCTACATCTG
CCTGGCAGCCATCTGGGCCTATGCTTCCTTCTGGACCACCATGCCCTTGGTAGGTCTGGG
GGACTACGTACCTGAGCCCTTCGGAACCTCGTGCACCCTGGACTGGTGGCTGGCCCAGGC
CTCGGTAGGGGGCCAGGTTTTCATCCTGAACATCCTCTTCTTCTGCCTCTTGCTCCCAAC
GGCTGTGATCGTGTTCTCCTACGTAAAGATCATTGCCAAGGTTAAGTCCTCTTCCAAAGA
AGTAGCTCATTTCGACAGTCGGATCCATAGCAGCCATGTGCTGGAAATGAAACTGACAAA
GGTAGCGATGTTGATTTGTGCTGGATTCCTGATTGCCTGGATTCCTTATGCAGTGGTGTC
TGTGTGGTCAGCTTTTGGAAGGCCAGACTCCATTCCCATACAGCTCTCTGTGGTGCCAAC
CCTACTTGCAAAATCTGCAGCGATGTACAATCCCATCATTTACCAAGTTATTGATTACAA
ATTTGCCTGTTGCCAAACTGGTGGTTTGAAAGCAACCAAGAAGAAGTCTCTGGAAGGCTT
CAGGCTGCACACCGTAACCACAGTCAGGAAGTCTTCTGCTGTGCTGGAAATTCATGAAGA
GTGGGAAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT