Restriction map for
pFN21AE9449
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AlwNI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
BsoBI BspEI BspMI BsrBI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII Cfr10I CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MslI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SpeI SphI Sse8387I
SspI SwaI Tth111I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AflIII 167 ACATGT A^C[GA][CT]GT
Alw44I 546 GTGCAC G^TGCAC
AocI 25 CCTCAGG CC^T[ACGT]AGG
ApaLI 546 GTGCAC G^TGCAC
AspHI 546 GTGCAC G[AT]GC[AT]^C
AvaII 488 GGACC G^G[AT]CC
BanI 867 GGTGCC G^G[CT][GA]CC
BanII 529 GAGCCC G[GA]GC[CT]^C
BfaI 176 CTAG C^TAG
BglI 463 GCCATCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 58 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 195 GAGACC GGTCTC(1/5)
BsgI 1000 CTGCAC GTGCAG(16/14)
BsiHKAI 546 GTGCAC G[AT]GC[AT]^C
BsmFI 515 GGGAC GGGAC(10/14)
Bsp1407I 899 TGTACA T^GTACA
BspDI 389 ATCGAT AT^CGAT
BspHI 1047 TCATGA T^CATGA
BspLU11I 167 ACATGT A^CATGT
BsrDI 666 CATTGC GCAATG(2/0)
BsrGI 899 TGTACA T^GTACA
BssSI 543 CTCGTG CACGAG(-5/-1)
BstAPI 883 GCAAAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 25 CCTCAGG CC^T[ACGT]AGG
CfoI 36 GCGC GCG^C
ClaI 389 ATCGAT AT^CGAT
Eco81I 25 CCTCAGG CC^T[ACGT]AGG
EcoNI 21 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 199 CCCGC CCCGC(4/6)
HaeII 35 AGCGCC [GA]GCGC^[CT]
HhaI 36 GCGC GCG^C
HinP1I 36 GCGC G^CGC
MaeI 176 CTAG C^TAG
MamI 58 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI 167 ACATGT [GA]CATG^[CT]
PflMI 729 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 841 GACTC GAGTC(4/5)
PshAI 372 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 890 CTGCAG CTGCA^G
RcaI 1047 TCATGA T^CATGA
SfcI 890 CTGCAG C^T[GA][CT]AG
SspBI 899 TGTACA T^GTACA
StuI 572 AGGCCT AGG^CCT
Tsp45I 287 GTCAC ^GT[GC]AC
Van91I 729 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 175 TCTAGA T^CTAGA
XcmI 491 CCACCATGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 934 AAATTT [GA]^AATT[CT]
1043 AAATTC
AfaI 523 GTAC GT^AC
900 GTAC
ApoI 934 AAATTT [GA]^AATT[CT]
1043 AAATTC
BamHI 63 GGATCC G^GATCC
716 GGATCC
BbsI 171 GTCTTC GAAGAC(2/6)
1026 GTCTTC
BmyI 529 GAGCCC G[AGT]GC[ACT]^C
546 GTGCAC
BpuAI 171 GTCTTC GAAGAC(2/6)
1026 GTCTTC
BsaAI 520 TACGTA [CT]AC^GT[GA]
655 TACGTA
BsmAI 195 GAGAC GTCTC(1/5)
981 GTCTC
Bsp1286I 529 GAGCCC G[AGT]GC[ACT]^C
546 GTGCAC
BsrI 557 ACTGG ACTGG(1/-1)
952 ACTGG
BstXI 494 CCATGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
948 CCAAACTGGTGG
BstYI 63 GGATCC [GA]^GATC[CT]
716 GGATCC
EcoT14I 500 CCTTGG C^C[AT][AT]GG
671 CCAAGG
MaeII 521 ACGT A^CGT
656 ACGT
MaeIII 287 GTCAC ^GT[ACGT]AC
1009 GTAAC
MflI 63 GGATCC [GA]^GATC[CT]
716 GGATCC
MseI 7 TTAA T^TAA
677 TTAA
RsaI 523 GTAC GT^AC
900 GTAC
SnaBI 520 TACGTA TAC^GTA
655 TACGTA
StyI 500 CCTTGG C^C[AT][AT]GG
671 CCAAGG
Tru9I 7 TTAA T^TAA
677 TTAA
TspRI 15 CACTG CA[GC]TG[ACGT][ACGT]^
806 CAGTG
XhoII 63 GGATCC [GA]^GATC[CT]
716 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BsiYI BslI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MboI MboII
MnlI MspA1I MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
TaqI TfiI TseI Tsp509I TthHB8I