GPR6 ORF clone pFN21AE9415

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Halotag ORF Clone pFN21AE9415

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Clone info.

Species

human

Clone Name

pFN21AE9415 Sequence (with vector) Restriction Map

Product ID

FHC22724

ORF Sequence

DNA sequence (1086 bp)
Protein sequence (362 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9415

Symbol

GPR6

Description

G protein-coupled receptor 6, transcript variant 2

Original Clone ID

cp99415

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1086 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 362 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5079.1 6e-91 100.0 GPR6 CCDS69172.1 6.1e-91 100.0 GPR6 CCDS303.1 1.7e-47 59.5 GPR3 CCDS9319.1 3.7e-46 58.6 GPR12 CCDS12407.1 1.9e-13 34.0 LPAR2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001151 2 20 PR00649 GPR6 orphan receptor IPR001151 20 37 PR00649 GPR6 orphan receptor IPR001151 42 59 PR00649 GPR6 orphan receptor IPR001151 59 73 PR00649 GPR6 orphan receptor IPR000723 74 85 PR00644 GPR orphan receptor IPR000723 97 108 PR00644 GPR orphan receptor IPR000276 107 128 PR00237 GPCR IPR000723 129 146 PR00644 GPR orphan receptor IPR000276 148 170 PR00237 GPCR IPR000723 167 178 PR00644 GPR orphan receptor IPR001151 178 192 PR00649 GPR6 orphan receptor IPR000276 184 205 PR00237 GPCR IPR000276 226 249 PR00237 GPCR IPR000723 249 264 PR00644 GPR orphan receptor IPR000723 264 280 PR00644 GPR orphan receptor IPR000276 277 301 PR00237 GPCR IPR001151 296 309 PR00649 GPR6 orphan receptor IPR000723 307 321 PR00644 GPR orphan receptor IPR000276 311 337 PR00237 GPCR IPR000723 330 341 PR00644 GPR orphan receptor IPR001151 342 352 PR00649 GPR6 orphan receptor HMMPfam IPR000276 89 329 PF00001 GPCR ProfileScan IPR017452 89 329 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 70 PAVNPWDVLLCVSGTVIAGENAL 92 SECONDARY 23 2 110 LVGSLATADLLAGCGLILHFVFQ 132 PRIMARY 23 3 147 FLVASFAASVSSLLAITVDRYLS 169 PRIMARY 23 4 186 HLLLAATWTVSLGLGLLPVLGWN 208 PRIMARY 23 5 224 RSHVALLSAAFFMVFGIMLHLYV 246 PRIMARY 23 6 279 GTLAVVLGTFGASWLPFAIYCVV 301 PRIMARY 23 7 318 ATYNSMINPIIYAFRNQEIQRAL 340 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005275 362 600553 100.0 100.0 NP_001273028 377 600553 100.0 100.0 NP_005272 330 600241 59.5 90.3 NP_005279 334 600752 58.6 88.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9415 Download

>KIEE9415  1086 bp
ATGAACGCGAGCGCCGCCTCGCTCAACGACTCCCAGGTGGTGGTAGTGGCGGCCGAAGGA
GCGGCGGCGGCGGCCACAGCAGCAGGGGGGCCGGACACGGGCGAATGGGGACCCCCTGCT
GCGGCGGCTCTAGGAGCCGGCGGCGGAGCTAATGGGTCTCTGGAGCTGTCCTCGCAGCTG
TCGGCTGGGCCACCGGGACTCCTGCTGCCAGCGGTGAATCCGTGGGACGTGCTCCTGTGC
GTGTCGGGGACAGTGATCGCTGGAGAAAACGCGCTGGTGGTGGCGCTCATCGCGTCCACT
CCGGCGCTGCGCACGCCCATGTTCGTGCTGGTAGGCAGCCTGGCCACCGCTGACCTGTTG
GCGGGCTGTGGCCTCATCTTGCACTTTGTGTTCCAGTACTTGGTGCCCTCGGAGACTGTG
AGTCTGCTCACGGTGGGCTTCCTCGTGGCCTCCTTCGCCGCCTCTGTCAGCAGCCTGCTG
GCCATTACGGTGGACCGCTACCTGTCCCTGTATAACGCGCTCACCTATTACTCGCGCCGG
ACCCTGTTGGGCGTGCACCTCCTGCTTGCCGCCACTTGGACCGTGTCCCTAGGCCTGGGG
CTGCTGCCCGTGCTGGGCTGGAACTGCCTGGCAGAGCGCGCCGCCTGCAGCGTGGTGCGC
CCGCTGGCGCGCAGCCACGTGGCTCTGCTCTCCGCCGCCTTCTTCATGGTCTTCGGCATC
ATGCTGCACCTGTACGTGCGCATCTGCCAGGTGGTCTGGCGCCACGCGCACCAGATCGCG
CTGCAGCAGCACTGCCTGGCGCCACCCCATCTCGCTGCCACCAGAAAGGGTGTGGGTACA
CTGGCTGTGGTGCTGGGCACTTTCGGCGCCAGCTGGCTGCCCTTCGCCATCTATTGCGTG
GTGGGCAGCCATGAGGACCCGGCGGTCTACACTTACGCCACCCTGCTGCCCGCCACCTAC
AACTCCATGATCAATCCCATCATCTATGCCTTCCGCAACCAGGAGATCCAGCGCGCCCTG
TGGCTCCTGCTCTGTGGCTGTTTCCAGTCCAAAGTGCCCTTTCGTTCCAGGTCTCCCAGC
GAGGTC

ORF Protein

Cloned ORF protein sequence for pFN21AE9415 Download

>KIEE9415  362 aa
MNASAASLNDSQVVVVAAEGAAAAATAAGGPDTGEWGPPAAAALGAGGGANGSLELSSQL
SAGPPGLLLPAVNPWDVLLCVSGTVIAGENALVVALIASTPALRTPMFVLVGSLATADLL
AGCGLILHFVFQYLVPSETVSLLTVGFLVASFAASVSSLLAITVDRYLSLYNALTYYSRR
TLLGVHLLLAATWTVSLGLGLLPVLGWNCLAERAACSVVRPLARSHVALLSAAFFMVFGI
MLHLYVRICQVVWRHAHQIALQQHCLAPPHLAATRKGVGTLAVVLGTFGASWLPFAIYCV
VGSHEDPAVYTYATLLPATYNSMINPIIYAFRNQEIQRALWLLLCGCFQSKVPFRSRSPS
EV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9415 Download

>pFN21AE9415  5791 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAACGCGAGCGCCGCCTCGCTCAACGACTCCCA
GGTGGTGGTAGTGGCGGCCGAAGGAGCGGCGGCGGCGGCCACAGCAGCAGGGGGGCCGGA
CACGGGCGAATGGGGACCCCCTGCTGCGGCGGCTCTAGGAGCCGGCGGCGGAGCTAATGG
GTCTCTGGAGCTGTCCTCGCAGCTGTCGGCTGGGCCACCGGGACTCCTGCTGCCAGCGGT
GAATCCGTGGGACGTGCTCCTGTGCGTGTCGGGGACAGTGATCGCTGGAGAAAACGCGCT
GGTGGTGGCGCTCATCGCGTCCACTCCGGCGCTGCGCACGCCCATGTTCGTGCTGGTAGG
CAGCCTGGCCACCGCTGACCTGTTGGCGGGCTGTGGCCTCATCTTGCACTTTGTGTTCCA
GTACTTGGTGCCCTCGGAGACTGTGAGTCTGCTCACGGTGGGCTTCCTCGTGGCCTCCTT
CGCCGCCTCTGTCAGCAGCCTGCTGGCCATTACGGTGGACCGCTACCTGTCCCTGTATAA
CGCGCTCACCTATTACTCGCGCCGGACCCTGTTGGGCGTGCACCTCCTGCTTGCCGCCAC
TTGGACCGTGTCCCTAGGCCTGGGGCTGCTGCCCGTGCTGGGCTGGAACTGCCTGGCAGA
GCGCGCCGCCTGCAGCGTGGTGCGCCCGCTGGCGCGCAGCCACGTGGCTCTGCTCTCCGC
CGCCTTCTTCATGGTCTTCGGCATCATGCTGCACCTGTACGTGCGCATCTGCCAGGTGGT
CTGGCGCCACGCGCACCAGATCGCGCTGCAGCAGCACTGCCTGGCGCCACCCCATCTCGC
TGCCACCAGAAAGGGTGTGGGTACACTGGCTGTGGTGCTGGGCACTTTCGGCGCCAGCTG
GCTGCCCTTCGCCATCTATTGCGTGGTGGGCAGCCATGAGGACCCGGCGGTCTACACTTA
CGCCACCCTGCTGCCCGCCACCTACAACTCCATGATCAATCCCATCATCTATGCCTTCCG
CAACCAGGAGATCCAGCGCGCCCTGTGGCTCCTGCTCTGTGGCTGTTTCCAGTCCAAAGT
GCCCTTTCGTTCCAGGTCTCCCAGCGAGGTCGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT