################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AvaI BamHI BanII BbvCI BcgI BciVI BfrI BglI BglII BlpI Bpu1102I BsaBI BsaWI BseAI BseRI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI Bst1107I BstBI BstEII BstF5I BstPI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FokI FseI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeIII MamI MfeI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tru9I Tsp45I Tsp509I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 925 GTCTAC GT^[AC][GT]AC AhdI 414 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 553 GTGCAC G^TGCAC AlwI 1005 GATCC GGATC(4/5) ApaLI 553 GTGCAC G^TGCAC AspEI 414 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 579 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 588 CCTAGG C^CTAGG BbrPI 676 CACGTG CAC^GTG BbsI 709 GTCTTC GAAGAC(2/6) BclI 968 TGATCA T^GATCA BlnI 588 CCTAGG C^CTAGG BpuAI 709 GTCTTC GAAGAC(2/6) BsgI 724 CTGCAC GTGCAG(16/14) BsiEI 50 CGGCCG CG[GA][CT]^CG BsrBI 59 GAGCGG CCGCTC(-3/-3) BsrFI 136 GCCGGC [GA]^CCGG[CT] BssSI 442 CTCGTG CACGAG(-5/-1) BstAPI 555 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 482 CCATTACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1004 AGATCC [GA]^GATC[CT] Cfr10I 136 GCCGGC [GA]^CCGG[CT] DraIII 382 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 916 GACCCGGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 220 CCGTGG C^C[GA][CT]GG EagI 50 CGGCCG C^GGCCG Eam1105I 414 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 50 CGGCCG C^GGCCG Eco52I 50 CGGCCG C^GGCCG EcoT14I 588 CCTAGG C^C[AT][AT]GG FbaI 968 TGATCA T^GATCA HgaI 292 GCGTC GACGC(5/10) MflI 1004 AGATCC [GA]^GATC[CT] NaeI 136 GCCGGC GCC^GGC NgoMIV 136 GCCGGC G^CCGGC PflFI 579 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 676 CACGTG CAC^GTG PmlI 676 CACGTG CAC^GTG ScaI 395 AGTACT AGT^ACT StuI 591 AGGCCT AGG^CCT StyI 588 CCTAGG C^C[AT][AT]GG TfiI 216 GAATC G^A[AT]TC Tth111I 579 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 1004 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AspHI 229 GTGCTC G[AT]GC[AT]^C 553 GTGCAC AviII 308 TGCGCA TGC^GCA 737 TGCGCA BalI 341 TGGCCA TGG^CCA 479 TGGCCA BcnI 193 CCGGG CC^[GC]GG 918 CCCGG BfaI 130 CTAG C^TAG 589 CTAG BpmI 160 CTGGAG CTGGAG(16/14) 260 CTGGAG BsaAI 676 CACGTG [CT]AC^GT[GA] 733 TACGTG BsaI 155 GGTCTC GGTCTC(1/5) 1070 GGTCTC BsiHKAI 229 GTGCTC G[AT]GC[AT]^C 553 GTGCAC DraII 108 GGGACCC [GA]G^G[ACGT]CC[CT] 914 AGGACCC EcoO109I 108 GGGACCC [GA]G^G[ACGT]CC[CT] 914 AGGACCC FspI 308 TGCGCA TGC^GCA 737 TGCGCA HphI 213 GGTGA GGTGA(8/7) 521 TCACC MaeI 130 CTAG C^TAG 589 CTAG MboII 701 TCTTC GAAGA(8/7) 710 TCTTC MluNI 341 TGGCCA TGG^CCA 479 TGGCCA MscI 341 TGGCCA TGG^CCA 479 TGGCCA NciI 193 CCGGG CC^[GC]GG 918 CCCGG PflMI 33 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 393 CCAGTACTTGG PpuMI 108 GGGACCC [GA]G^G[AT]CC[CT] 914 AGGACCC PstI 645 CTGCAG CTGCA^G 781 CTGCAG PvuII 175 CAGCTG CAG^CTG 870 CAGCTG SfaNI 716 GCATC GCATC(5/9) 740 GCATC SfcI 645 CTGCAG C^T[GA][CT]AG 781 CTGCAG Van91I 33 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 393 CCAGTACTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaII BanI BbeI BbvI BmyI BsaHI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr13I DpnII EaeI EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI KasI MaeII MboI MnlI MslI MspA1I MspI MvaI MvnI MwoI NarI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfoI TseI TspRI XcmI