DDX52 ORF clone pFN21AE5767

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE5767

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Clone info.

Species

human

Clone Name

pFN21AE5767 Sequence (with vector) Restriction Map

Product ID

FHC25988

ORF Sequence

DNA sequence (1797 bp)
Protein sequence (599 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5767

Symbol

DDX52

Alias : HUSSY19, ROK1

Description

DEAD (Asp-Glu-Ala-Asp) box polypeptide 52, transcript variant 1

Original Clone ID

oc95767

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1797 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 599 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11323.1 1.8e-207 100.0 DDX52 CCDS34240.1 3.2e-36 29.9 DDX46 CCDS75306.1 3.2e-36 29.9 DDX46 CCDS4977.1 1e-35 32.8 DDX43 CCDS54854.1 1e-33 30.2 DDX4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR011545 189 362 PF00270 DNA/RNA helicase IPR001650 432 507 PF00271 Helicase HMMSmart IPR014001 184 389 SM00487 DEAD-like helicase IPR001650 426 507 SM00490 Helicase ProfileScan IPR014014 165 193 PS51195 RNA helicase IPR014001 196 374 PS51192 DEAD-like helicase IPR001650 385 546 PS51194 Helicase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_008941 599 612500 100.0 100.0 XP_011522534 581 612500 100.0 97.0 NP_001278405 491 612500 100.0 82.0 XP_011534228 604 606286 31.7 85.5 XP_011541799 678 605281 30.2 89.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5767 Download

>KIEE5767  1797 bp
ATGGACGTCCACGATCTCTTTCGCCGGCTCGGCGCGGGGGCCAAATTCGACACGAGACGC
TTCTCGGCAGACGCAGCTCGATTCCAGATAGGAAAAAGGAAATATGACTTTGATTCTTCG
GAGGTGCTTCAGGGACTGGACTTTTTTGGAAACAAGAAGTCTGTCCCAGGTGTGTGTGGA
GCATCACAAACACATCAGAAGCCCCAAAATGGAGAGAAAAAAGAAGAGAGCCTAACTGAA
AGGAAGAGGGAGCAGAGCAAGAAAAAAAGGAAGACGATGACTTCAGAAATTGCTTCCCAA
GAAGAAGGTGCTACTATACAGTGGATGTCATCTGTAGAAGCAAAGATTGAAGACAAAAAA
GTTCAGAGAGAAAGTAAACTAACTTCCGGAAAGTTGGAGAATCTCAGAAAAGAAAAGATA
AACTTCTTGCGGAATAAACACAAAATTCACGTCCAAGGAACCGATCTTCCTGACCCAATT
GCTACATTTCAGCAACTTGACCAGGAATATAAAATCAATTCTCGACTACTTCAGAACATT
CTAGATGCAGGTTTCCAAATGCCTACGCCAATCCAAATGCAAGCCATCCCAGTTATGCTG
CATGGTCGGGAACTTCTGGCTTCTGCTCCAACTGGATCTGGAAAAACATTAGCTTTTAGC
ATTCCTATTTTAATGCAGCTGAAACAACCCGCAAATAAAGGCTTCAGAGCCCTGATTATA
TCACCAACACGAGAACTTGCCAGCCAGATTCACAGAGAGTTAATAAAAATTTCTGAGGGA
ACAGGATTCAGGATACACATGATCCACAAAGCAGCAGTGGCAGCCAAGAAATTTGGACCT
AAATCATCTAAAAAGTTTGATATTCTTGTGACTACTCCAAATCGACTAATCTATTTATTA
AAGCAAGATCCCCCCGGAATCGACCTAGCAAGTGTTGAGTGGCTTGTAGTAGACGAATCA
GATAAACTGTTTGAAGATGGCAAAACTGGGTTCAGAGACCAGCTGGCTTCCATTTTCCTG
GCCTGCACATCCCACAAGGTCCGAAGAGCTATGTTCAGTGCAACTTTTGCATATGATGTT
GAACAGTGGTGCAAACTCAACCTGGACAATGTCATCAGTGTGTCCATTGGAGCAAGGAAT
TCTGCAGTAGAAACTGTAGAACAAGAGCTTCTCTTTGTTGGATCTGAGACCGGAAAACTT
CTGGCCGTGAGAGAACTTGTTAAAAAGGGTTTCAATCCACCTGTTCTTGTTTTTGTTCAG
TCCATTGAAAGGGCTAAAGAACTTTTTCATGAGCTCATATATGAAGGTATTAATGTGGAT
GTTATTCATGCAGAGAGAACACAACAACAGAGAGATAACACAGTCCACAGTTTCAGAGCA
GGAAAAATCTGGGTTCTGATTTGTACAGCCTTGCTAGCAAGAGGGATTGATTTTAAAGGT
GTGAACTTGGTGATCAACTATGACTTTCCAACTAGCTCAGTGGAATATATCCACAGGATA
GGTCGAACTGGAAGAGCAGGGAATAAGGGAAAAGCAATTACATTTTTCACTGAGGATGAT
AAGCCATTATTAAGAAGCGTTGCTAATGTTATACAGCAGGCTGGGTGTCCTGTACCAGAA
TACATAAAAGGTTTTCAGAAACTACTAAGCAAACAAAAGAAAAAGATGATTAAGAAACCA
TTGGAAAGGGAGAGCATTAGTACAACTCCAAAATGTTTCTTAGAAAAAGCTAAGGATAAA
CAGAAAAAGGTCACTGGTCAGAACAGCAAGAAGAAAGTAGCTCTTGAAGACAAAAGT

ORF Protein

Cloned ORF protein sequence for pFN21AE5767 Download

>KIEE5767  599 aa
MDVHDLFRRLGAGAKFDTRRFSADAARFQIGKRKYDFDSSEVLQGLDFFGNKKSVPGVCG
ASQTHQKPQNGEKKEESLTERKREQSKKKRKTMTSEIASQEEGATIQWMSSVEAKIEDKK
VQRESKLTSGKLENLRKEKINFLRNKHKIHVQGTDLPDPIATFQQLDQEYKINSRLLQNI
LDAGFQMPTPIQMQAIPVMLHGRELLASAPTGSGKTLAFSIPILMQLKQPANKGFRALII
SPTRELASQIHRELIKISEGTGFRIHMIHKAAVAAKKFGPKSSKKFDILVTTPNRLIYLL
KQDPPGIDLASVEWLVVDESDKLFEDGKTGFRDQLASIFLACTSHKVRRAMFSATFAYDV
EQWCKLNLDNVISVSIGARNSAVETVEQELLFVGSETGKLLAVRELVKKGFNPPVLVFVQ
SIERAKELFHELIYEGINVDVIHAERTQQQRDNTVHSFRAGKIWVLICTALLARGIDFKG
VNLVINYDFPTSSVEYIHRIGRTGRAGNKGKAITFFTEDDKPLLRSVANVIQQAGCPVPE
YIKGFQKLLSKQKKKMIKKPLERESISTTPKCFLEKAKDKQKKVTGQNSKKKVALEDKS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5767 Download

>pFN21AE5767  6502 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGACGTCCACGATCTCTTTCGCCGGCTCGGCGC
GGGGGCCAAATTCGACACGAGACGCTTCTCGGCAGACGCAGCTCGATTCCAGATAGGAAA
AAGGAAATATGACTTTGATTCTTCGGAGGTGCTTCAGGGACTGGACTTTTTTGGAAACAA
GAAGTCTGTCCCAGGTGTGTGTGGAGCATCACAAACACATCAGAAGCCCCAAAATGGAGA
GAAAAAAGAAGAGAGCCTAACTGAAAGGAAGAGGGAGCAGAGCAAGAAAAAAAGGAAGAC
GATGACTTCAGAAATTGCTTCCCAAGAAGAAGGTGCTACTATACAGTGGATGTCATCTGT
AGAAGCAAAGATTGAAGACAAAAAAGTTCAGAGAGAAAGTAAACTAACTTCCGGAAAGTT
GGAGAATCTCAGAAAAGAAAAGATAAACTTCTTGCGGAATAAACACAAAATTCACGTCCA
AGGAACCGATCTTCCTGACCCAATTGCTACATTTCAGCAACTTGACCAGGAATATAAAAT
CAATTCTCGACTACTTCAGAACATTCTAGATGCAGGTTTCCAAATGCCTACGCCAATCCA
AATGCAAGCCATCCCAGTTATGCTGCATGGTCGGGAACTTCTGGCTTCTGCTCCAACTGG
ATCTGGAAAAACATTAGCTTTTAGCATTCCTATTTTAATGCAGCTGAAACAACCCGCAAA
TAAAGGCTTCAGAGCCCTGATTATATCACCAACACGAGAACTTGCCAGCCAGATTCACAG
AGAGTTAATAAAAATTTCTGAGGGAACAGGATTCAGGATACACATGATCCACAAAGCAGC
AGTGGCAGCCAAGAAATTTGGACCTAAATCATCTAAAAAGTTTGATATTCTTGTGACTAC
TCCAAATCGACTAATCTATTTATTAAAGCAAGATCCCCCCGGAATCGACCTAGCAAGTGT
TGAGTGGCTTGTAGTAGACGAATCAGATAAACTGTTTGAAGATGGCAAAACTGGGTTCAG
AGACCAGCTGGCTTCCATTTTCCTGGCCTGCACATCCCACAAGGTCCGAAGAGCTATGTT
CAGTGCAACTTTTGCATATGATGTTGAACAGTGGTGCAAACTCAACCTGGACAATGTCAT
CAGTGTGTCCATTGGAGCAAGGAATTCTGCAGTAGAAACTGTAGAACAAGAGCTTCTCTT
TGTTGGATCTGAGACCGGAAAACTTCTGGCCGTGAGAGAACTTGTTAAAAAGGGTTTCAA
TCCACCTGTTCTTGTTTTTGTTCAGTCCATTGAAAGGGCTAAAGAACTTTTTCATGAGCT
CATATATGAAGGTATTAATGTGGATGTTATTCATGCAGAGAGAACACAACAACAGAGAGA
TAACACAGTCCACAGTTTCAGAGCAGGAAAAATCTGGGTTCTGATTTGTACAGCCTTGCT
AGCAAGAGGGATTGATTTTAAAGGTGTGAACTTGGTGATCAACTATGACTTTCCAACTAG
CTCAGTGGAATATATCCACAGGATAGGTCGAACTGGAAGAGCAGGGAATAAGGGAAAAGC
AATTACATTTTTCACTGAGGATGATAAGCCATTATTAAGAAGCGTTGCTAATGTTATACA
GCAGGCTGGGTGTCCTGTACCAGAATACATAAAAGGTTTTCAGAAACTACTAAGCAAACA
AAAGAAAAAGATGATTAAGAAACCATTGGAAAGGGAGAGCATTAGTACAACTCCAAAATG
TTTCTTAGAAAAAGCTAAGGATAAACAGAAAAAGGTCACTGGTCAGAACAGCAAGAAGAA
AGTAGCTCTTGAAGACAAAAGTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT