(Circular) (Six-base) MAPSORT of: pFN21AE5767.seq Check: 7999 from: 1 to: 6502 pFN21AE5767 6502 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2013 4312 278 Size: 53 83 186 936 477 2299 2468 Fragments arranged by size: 2468 2299 936 477 186 83 53 AccI GT'mk_AC Cuts at: 1937 2955 3845 1937 Size: 1018 890 4594 Fragments arranged by size: 4594 1018 890 Acc65I G'GTAC_C Cuts at: 3823 3823 Size: 6502 AclI AA'CG_TT Cuts at: 1563 4629 5002 1563 Size: 3066 373 3063 Fragments arranged by size: 3066 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2117 2271 2519 2692 4565 5949 6217 1632 Size: 485 154 248 173 1873 1384 268 1917 Fragments arranged by size: 1917 1873 1384 485 268 248 173 154 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6305 AflIII A'CryG_T Cuts at: 1203 5401 6378 1203 Size: 4198 977 1327 Fragments arranged by size: 4198 1327 977 AgeI A'CCGG_T Cuts at: 5333 5333 Size: 6502 AhdI GACnn_n'nnGTC Cuts at: 5231 5231 Size: 6502 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6502 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6502 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2455 2487 2455 Size: 32 6470 AlwNI CAG_nnn'CTG Cuts at: 2140 4245 5817 2140 Size: 2105 1572 2825 Fragments arranged by size: 2825 2105 1572 ApaLI G'TGCA_C Cuts at: 4559 5715 4559 Size: 1156 5346 ApoI r'AATT_y Cuts at: 1493 2048 2448 2772 2834 3142 3811 4037 4189 Size: 555 400 324 62 308 669 226 152 Cuts at: 4189 6298 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 669 555 400 324 308 226 152 62 AseI AT'TA_AT Cuts at: 160 1032 3315 5056 160 Size: 872 2283 1741 1606 Fragments arranged by size: 2283 1741 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 AvaI C'yCGr_G Cuts at: 1870 1943 3827 1870 Size: 73 1884 4545 Fragments arranged by size: 4545 1884 73 BamHI G'GATC_C Cuts at: 3832 4199 3832 Size: 367 6135 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3823 5278 618 Size: 325 217 627 2036 1455 1842 Fragments arranged by size: 2036 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2716 3300 3821 1391 Size: 1325 584 521 4072 Fragments arranged by size: 4072 1325 584 521 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2282 2361 3798 928 Size: 971 383 79 1437 3632 Fragments arranged by size: 3632 1437 971 383 79 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4693 4727 1903 Size: 34 2756 34 3678 Fragments arranged by size: 3678 2756 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2790 4394 5604 2790 Size: 1604 1210 3688 Fragments arranged by size: 3688 1604 1210 BclI T'GATC_A Cuts at: 1548 3456 1548 Size: 1908 4594 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2034 5113 136 Size: 107 122 71 631 605 362 3079 1525 Fragments arranged by size: 3079 1525 631 605 362 122 107 71 BglII A'GATC_T Cuts at: 6497 6497 Size: 6502 BlpI GC'TnA_GC Cuts at: 3919 3919 Size: 6502 Bme1580I G_kGCm'C Cuts at: 1790 4563 5719 1790 Size: 2773 1156 2573 Fragments arranged by size: 2773 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2455 1318 Size: 1137 5365 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2588 3000 4233 Size: 416 53 83 399 24 1171 412 1233 Cuts at: 4233 5191 442 Size: 958 1753 Fragments arranged by size: 1753 1233 1171 958 416 412 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3422 1056 Size: 2366 4136 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5162 1239 Size: 3923 2579 Bpu10I CC'TnA_GC Cuts at: 3735 3735 Size: 6502 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4627 5492 5790 4627 Size: 865 298 5339 Fragments arranged by size: 5339 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2994 3185 5165 882 Size: 617 1495 191 1980 2219 Fragments arranged by size: 2219 1980 1495 617 191 BsaAI yAC'GTr Cuts at: 493 1204 4293 493 Size: 711 3089 2702 Fragments arranged by size: 3089 2702 711 BsaBI GATnn'nnATC Cuts at: 6496 6496 Size: 6502 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2010 4309 4691 5326 Size: 53 83 186 936 477 2299 382 635 Cuts at: 5326 275 Size: 1451 Fragments arranged by size: 2299 1451 936 635 477 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2390 3194 4935 5333 5607 5754 6165 2390 Size: 804 1741 398 274 147 411 2727 Fragments arranged by size: 2727 1741 804 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6277 Fragments arranged by size: 6277 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6502 BseYI C'CCAG_C Cuts at: 3605 5705 6201 3605 Size: 2100 496 3906 Fragments arranged by size: 3906 2100 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3013 1163 Size: 573 1277 4652 Fragments arranged by size: 4652 1277 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3952 4713 5741 1540 Size: 275 186 1951 761 1028 2301 Fragments arranged by size: 2301 1951 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3300 4563 4648 5719 3300 Size: 1263 85 1071 4083 Fragments arranged by size: 4083 1263 1071 85 BsmI GAATG_Cn' Cuts at: 2664 4013 4106 2664 Size: 1349 93 5060 Fragments arranged by size: 5060 1349 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2053 2053 Size: 6502 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2716 3300 3821 4563 4648 5719 1391 Size: 399 926 584 521 742 85 1071 2174 Fragments arranged by size: 2174 1071 926 742 584 521 399 85 BspEI T'CCGG_A Cuts at: 2390 6165 2390 Size: 3775 2727 BspHI T'CATG_A Cuts at: 3292 4391 6121 3292 Size: 1099 1730 3673 Fragments arranged by size: 3673 1730 1099 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2543 3857 844 Size: 1699 1314 3489 Fragments arranged by size: 3489 1699 1314 BsrBI CCG'CTC Cuts at: 1394 1823 4389 1394 Size: 429 2566 3507 Fragments arranged by size: 3507 2566 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4997 5171 65 Size: 1183 3749 174 1396 Fragments arranged by size: 3749 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2028 5146 5333 1700 Size: 110 218 3118 187 2869 Fragments arranged by size: 3118 2869 218 187 110 BsrGI T'GTAC_A Cuts at: 96 3407 96 Size: 3311 3191 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6502 BssSI C'ACGA_G Cuts at: 2056 2733 4562 5574 2056 Size: 677 1829 1012 2984 Fragments arranged by size: 2984 1829 1012 677 BstAPI GCAn_nnn'nTGC Cuts at: 794 3071 794 Size: 2277 4225 BstBI TT'CG_AA Cuts at: 5306 5306 Size: 6502 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6502 BstXI CCAn_nnnn'nTGG Cuts at: 3022 3022 Size: 6502 BstYI r'GATC_y Cuts at: 1972 2639 2911 3185 3832 4199 4584 4601 6053 Size: 667 272 274 647 367 385 17 1452 Cuts at: 6053 6139 6151 6497 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 667 647 385 367 346 274 272 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5011 Fragments arranged by size: 5011 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5763 Fragments arranged by size: 5763 683 56 BtsI GCAGTG_nn' Cuts at: 2826 4018 4812 4832 2826 Size: 1192 794 20 4496 Fragments arranged by size: 4496 1192 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6502 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6467 DraI TTT'AAA Cuts at: 3439 3806 3439 Size: 367 6135 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5509 809 Size: 507 4193 1802 Fragments arranged by size: 4193 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3207 3949 4838 Size: 54 1425 50 275 18 1377 742 889 Cuts at: 4838 6467 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1377 889 742 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3949 1537 Size: 275 2137 4090 Fragments arranged by size: 4090 2137 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2223 2243 3043 3511 4432 1353 Size: 870 20 800 468 921 3423 Fragments arranged by size: 3423 921 870 800 468 20 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5089 5463 5609 387 Size: 1064 138 3500 374 146 1280 Fragments arranged by size: 3500 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3298 3298 Size: 6502 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2271 2519 2692 4565 5162 5949 Size: 393 485 154 248 173 1873 597 787 Cuts at: 5949 6217 1239 Size: 268 1524 Fragments arranged by size: 1873 1524 787 597 485 393 268 248 173 154 EcoRI G'AATT_C Cuts at: 3142 3811 4189 3142 Size: 669 378 5455 Fragments arranged by size: 5455 669 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2443 3803 2443 Size: 1360 5142 FspI TGC'GCA Cuts at: 5008 5008 Size: 6502 HaeII r_GCGC'y Cuts at: 1678 5649 1678 Size: 3971 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5143 5175 5217 5249 1373 Size: 32 135 32 3571 32 42 32 2626 Fragments arranged by size: 3571 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3846 4092 669 Size: 988 281 1908 246 3079 Fragments arranged by size: 3079 1908 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6502 HpaI GTT'AAC Cuts at: 4092 4092 Size: 6502 KpnI G_GTAC'C Cuts at: 3827 3827 Size: 6502 MfeI C'AATT_G Cuts at: 2481 4101 2481 Size: 1620 4882 MluI A'CGCG_T Cuts at: 6378 6378 Size: 6502 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2379 2657 3163 3497 5616 5800 1305 Size: 1074 278 506 334 2119 184 2007 Fragments arranged by size: 2119 2007 1074 506 334 278 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4680 Fragments arranged by size: 4680 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3122 4460 4819 4978 518 Size: 648 1956 1338 359 159 2042 Fragments arranged by size: 2042 1956 1338 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2683 3007 3918 4595 5743 5988 1606 Size: 1077 324 911 677 1148 245 2120 Fragments arranged by size: 2120 1148 1077 911 677 324 245 NaeI GCC'GGC Cuts at: 2030 2030 Size: 6502 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5011 Fragments arranged by size: 5011 939 552 NdeI CA'TA_TG Cuts at: 387 3076 387 Size: 2689 3813 NgoMIV G'CCGG_C Cuts at: 2028 2028 Size: 6502 NheI G'CTAG_C Cuts at: 1052 3418 1052 Size: 2366 4136 NotI GC'GGCC_GC Cuts at: 3949 3949 Size: 6502 NspI r_CATG'y Cuts at: 1132 1229 3860 5405 1132 Size: 97 2631 1545 2229 Fragments arranged by size: 2631 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6502 PciI A'CATG_T Cuts at: 5401 5401 Size: 6502 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6502 PmeI GTTT'AAAC Cuts at: 3806 3806 Size: 6502 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4571 4603 6084 6116 4571 Size: 32 1481 32 4957 Fragments arranged by size: 4957 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6502 PsiI TTA'TAA Cuts at: 4072 4072 Size: 6502 PstI C_TGCA'G Cuts at: 830 3151 3854 830 Size: 2321 703 3478 Fragments arranged by size: 3478 2321 703 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6502 PvuII CAG'CTG Cuts at: 2683 3007 2683 Size: 324 6178 SacI G_AGCT'C Cuts at: 3300 3300 Size: 6502 SalI G'TCGA_C Cuts at: 1936 3844 1936 Size: 1908 4594 SapI GCTCTTCn'nnn_ Cuts at: 3043 3511 3043 Size: 468 6034 SbfI CC_TGCA'GG Cuts at: 3854 3854 Size: 6502 ScaI AGT'ACT Cuts at: 4750 6462 4750 Size: 1712 4790 SfcI C'TryA_G Cuts at: 826 1045 2337 3147 3159 3850 4985 5666 5857 Size: 219 1292 810 12 691 1135 681 191 Cuts at: 5857 826 Size: 1471 Fragments arranged by size: 1471 1292 1135 810 691 681 219 191 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6502 SmaI CCC'GGG Cuts at: 1872 3829 1872 Size: 1957 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4606 5507 5769 820 Size: 197 926 2663 901 262 1553 Fragments arranged by size: 2663 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4293 493 Size: 3800 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6502 SphI G_CATG'C Cuts at: 1132 3860 1132 Size: 2728 3774 SspI AAT'ATT Cuts at: 5 52 4426 5 Size: 47 4374 2081 Fragments arranged by size: 4374 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6502 StyI C'CwwG_G Cuts at: 513 1065 2004 2458 3941 513 Size: 552 939 454 1483 3074 Fragments arranged by size: 3074 1483 939 552 454 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3597 4529 4546 4699 4884 5329 325 Size: 1255 2017 932 17 153 185 445 1498 Fragments arranged by size: 2017 1498 1255 932 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3407 3704 4748 Size: 274 80 33 51 1443 1430 297 1044 Cuts at: 4748 6460 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1430 1044 297 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6462 6494 6462 Size: 32 6470 Tth111I GACn'n_nGTC Cuts at: 1316 3113 1316 Size: 1797 4705 XbaI T'CTAG_A Cuts at: 2545 3838 2545 Size: 1293 5209 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6502 XmaI C'CCGG_G Cuts at: 1870 3827 1870 Size: 1957 4545 XmnI GAAnn'nnTTC Cuts at: 3288 4631 3288 Size: 1343 5159 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2011 4310 276 Size: 53 83 186 936 477 2299 2468 Fragments arranged by size: 2468 2299 936 477 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI