################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## Acc65I AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BseRI BsiEI BsiWI BsoBI BspDI BspLU11I BsrBI BsrDI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MamI MluI MluNI MscI NarI NcoI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AatII 4 GACGTC GACGT^C AccI 949 GTAGAC GT^[AC][GT]AC AccII 33 CGCG CG^CG AccIII 385 TCCGGA T^CCGGA AcyI 4 GACGTC G[GA]^CG[CT]C AlwNI 130 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG AseI 1309 ATTAAT AT^TAAT AsnI 1309 ATTAAT AT^TAAT Asp700I 1279 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1291 GAGCTC G[AT]GC[AT]^C AspI 1105 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC BciVI 791 GGATAC GTATCC(6/5) BclI 1451 TGATCA T^GATCA BcnI 913 CCCGG CC^[GC]GG BglI 23 GCCGGCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 4 GACGTC G[GA]^CG[CT]C BseAI 385 TCCGGA T^CCGGA BsgI 1023 CTGCAC GTGCAG(16/14) BsiHKAI 1291 GAGCTC G[AT]GC[AT]^C BsmBI 54 GAGACG CGTCTC(1/5) BsmI 659 GCATTC GAATGC(1/-1) Bsp1407I 1402 TGTACA T^GTACA BspEI 385 TCCGGA T^CCGGA BspHI 1287 TCATGA T^CATGA BspMI 547 GCAGGT ACCTGC(4/8) BsrFI 23 GCCGGC [GA]^CCGG[CT] BsrGI 1402 TGTACA T^GTACA BstAPI 1060 GCAACTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 33 CGCG CG^CG BstXI 1010 CCATTTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 32 GCGC GCG^C Cfr10I 23 GCCGGC [GA]^CCGG[CT] DraI 1432 TTTAAA TTT^AAA EaeI 1202 TGGCCG [CT]^GGCC[GA] EcoRI 1137 GAATTC G^AATTC EcoT14I 453 CCAAGG C^C[AT][AT]GG FbaI 1451 TGATCA T^GATCA HhaI 32 GCGC GCG^C Hin1I 4 GACGTC G[GA]^CG[CT]C HinP1I 32 GCGC G^CGC MfeI 476 CAATTG C^AATTG MroI 385 TCCGGA T^CCGGA MslI 1113 CATCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 476 CAATTG C^AATTG MvnI 33 CGCG CG^CG NaeI 23 GCCGGC GCC^GGC NciI 913 CCCGG CC^[GC]GG NdeI 1070 CATATG CA^TATG NgoMIV 23 GCCGGC G^CCGGC NheI 1413 GCTAGC G^CTAGC PflFI 1105 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 1309 ATTAAT AT^TAAT PstI 1142 CTGCAG CTGCA^G RcaI 1287 TCATGA T^CATGA SacI 1291 GAGCTC GAGCT^C SspBI 1402 TGTACA T^GTACA StyI 453 CCAAGG C^C[AT][AT]GG Tth111I 1105 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 540 TCTAGA T^CTAGA XmnI 1279 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AvaII 835 GGACC G^G[AT]CC 1038 GGTCC BanII 707 GAGCCC G[GA]GC[CT]^C 1291 GAGCTC BmyI 707 GAGCCC G[AGT]GC[ACT]^C 1291 GAGCTC BsaI 995 GAGACC GGTCTC(1/5) 1186 GAGACC BsaWI 385 TCCGGA [AT]^CCGG[AT] 1189 ACCGGA BsiYI 202 CCCCAAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1262 CCATTGAAAGG BslI 202 CCCCAAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1262 CCATTGAAAGG BsmFI 132 GGGAC GGGAC(10/14) 163 GTCCC Bsp1286I 707 GAGCCC G[AGT]GC[ACT]^C 1291 GAGCTC BssSI 51 CACGAG CACGAG(-5/-1) 728 CACGAG FauI 688 CCCGC CCCGC(4/6) 34 GCGGG HgaI 56 GACGC GACGC(5/10) 70 GACGC HphI 1449 GGTGA GGTGA(8/7) 721 TCACC MaeII 5 ACGT A^CGT 449 ACGT MaeIII 868 GTGAC ^GT[ACGT]AC 1750 GTCAC MspA1I 676 CAGCTG C[AC]G^C[GT]G 1000 CAGCTG NlaIV 37 GGGGCC GG[ACGT]^[ACGT]CC 457 GGAACC PvuII 676 CAGCTG CAG^CTG 1000 CAGCTG SapI 1043 GAAGAGC GCTCTTC(1/4) 1511 GAAGAGC SfaNI 544 GATGC GCATC(5/9) 181 GCATC Tsp45I 868 GTGAC ^GT[GC]AC 1750 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BbsI BbvI BfaI BpuAI BsaJI BsmAI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeI MboI MboII MflI MnlI MseI MspI MvaI MwoI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII