RNF148 ORF clone pFN21AE4054

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE4054

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Clone info.

Species

human

Clone Name

pFN21AE4054 Sequence (with vector) Restriction Map

Product ID

FHC31189

ORF Sequence

DNA sequence (915 bp)
Protein sequence (305 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4054

Symbol

RNF148

Description

ring finger protein 148

Original Clone ID

cp94054

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 915 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 305 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS47692.1 4.6e-118 100.0 RNF148 CCDS5784.1 2.5e-65 55.4 RNF133 CCDS14520.1 3.7e-47 48.4 RNF128 CCDS14521.1 9.7e-44 51.3 RNF128 CCDS34065.1 2e-35 36.9 RNF150 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003137 95 165 PF02225 Protease-associated domain IPR018957 258 298 PF00097 Zinc finger HMMSmart IPR001841 258 298 SM00184 Zinc finger ProfileScan IPR001841 258 299 PS50089 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_078815 402 300439 48.4 87.2 NP_919445 428 300439 51.3 94.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4054 Download

>KIEE4054  915 bp
ATGAGCTTCCTTAGAATTACCCCTTCGACGCATAGTTCTGTTTCATCTGGACTTTTGAGG
CTTAGTATCTTTCTACTACTTAGCTTTCCTGACTCAAACGGAAAAGCCATTTGGACAGCT
CACCTGAATATAACATTTCAGGTTGGAAATGAGATCACATCGGAATTAGGAGAGAGTGGA
GTGTTCGGGAATCATTCTCCTCTGGAAAGGGTGTCTGGTGTGGTGGCACTTCCTGAAGGA
TGGAATCAGAATGCCTGTCATCCTTTGACCAATTTCAGCAGGCCCAAACAGGCAGACTCA
TGGCTGGCCCTCATCGAACGTGGAGGCTGTACTTTTACACATAAAATCAACGTGGCAGCA
GAGAAGGGAGCAAATGGGGTGATCATCTACAACTATCAAGGTACGGGCAGTAAAGTATTT
CCCATGTCTCACCAGGGGACGGAAAATATAGTCGCGGTGATGATAAGCAACCTGAAAGGC
ATGGAAATTTTGCACTCGATTCAGAAAGGAGTCTATGTGACAGTCATCATTGAAGTGGGG
AGAATGCACATGCAGTGGGTGAGCCATTACATCATGTATCTATTTACCTTCCTGGCTGCC
ACAATTGCCTACTTTTACTTAGATTGCGTCTGGAGACTTACACCTAGAGTGCCCAATTCT
TTCACCAGGAGGCGAAGTCAAATAAAGACAGATGTGAAGAAAGCTATTGACCAGCTTCAA
CTGCGAGTTCTCAAAGAAGGGGATGAGGAATTAGACCTAAATGAAGACAACTGTGTTGTT
TGCTTTGACACATACAAACCCCAGGATGTAGTACGCATTTTAACTTGCAAACATTTTTTC
CATAAGGCATGCATTGACCCCTGGCTTTTAGCCCATAGGACATGTCCCATGTGCAAGTGT
GACATCCTGAAAACT

ORF Protein

Cloned ORF protein sequence for pFN21AE4054 Download

>KIEE4054  305 aa
MSFLRITPSTHSSVSSGLLRLSIFLLLSFPDSNGKAIWTAHLNITFQVGNEITSELGESG
VFGNHSPLERVSGVVALPEGWNQNACHPLTNFSRPKQADSWLALIERGGCTFTHKINVAA
EKGANGVIIYNYQGTGSKVFPMSHQGTENIVAVMISNLKGMEILHSIQKGVYVTVIIEVG
RMHMQWVSHYIMYLFTFLAATIAYFYLDCVWRLTPRVPNSFTRRRSQIKTDVKKAIDQLQ
LRVLKEGDEELDLNEDNCVVCFDTYKPQDVVRILTCKHFFHKACIDPWLLAHRTCPMCKC
DILKT

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4054 Download

>pFN21AE4054  5620 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGCTTCCTTAGAATTACCCCTTCGACGCATAG
TTCTGTTTCATCTGGACTTTTGAGGCTTAGTATCTTTCTACTACTTAGCTTTCCTGACTC
AAACGGAAAAGCCATTTGGACAGCTCACCTGAATATAACATTTCAGGTTGGAAATGAGAT
CACATCGGAATTAGGAGAGAGTGGAGTGTTCGGGAATCATTCTCCTCTGGAAAGGGTGTC
TGGTGTGGTGGCACTTCCTGAAGGATGGAATCAGAATGCCTGTCATCCTTTGACCAATTT
CAGCAGGCCCAAACAGGCAGACTCATGGCTGGCCCTCATCGAACGTGGAGGCTGTACTTT
TACACATAAAATCAACGTGGCAGCAGAGAAGGGAGCAAATGGGGTGATCATCTACAACTA
TCAAGGTACGGGCAGTAAAGTATTTCCCATGTCTCACCAGGGGACGGAAAATATAGTCGC
GGTGATGATAAGCAACCTGAAAGGCATGGAAATTTTGCACTCGATTCAGAAAGGAGTCTA
TGTGACAGTCATCATTGAAGTGGGGAGAATGCACATGCAGTGGGTGAGCCATTACATCAT
GTATCTATTTACCTTCCTGGCTGCCACAATTGCCTACTTTTACTTAGATTGCGTCTGGAG
ACTTACACCTAGAGTGCCCAATTCTTTCACCAGGAGGCGAAGTCAAATAAAGACAGATGT
GAAGAAAGCTATTGACCAGCTTCAACTGCGAGTTCTCAAAGAAGGGGATGAGGAATTAGA
CCTAAATGAAGACAACTGTGTTGTTTGCTTTGACACATACAAACCCCAGGATGTAGTACG
CATTTTAACTTGCAAACATTTTTTCCATAAGGCATGCATTGACCCCTGGCTTTTAGCCCA
TAGGACATGTCCCATGTGCAAGTGTGACATCCTGAAAACTGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT