################################## # NO CUT ENZYMES ( 186 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MamI MflI MluI MluNI MroI MscI MspA1I MspI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AccII 453 CGCG CG^CG AciI 454 GCGG CCGC(-3/-1) AcsI 485 AAATTT [GA]^AATT[CT] AflIII 880 ACATGT A^C[GA][CT]GT ApoI 485 AAATTT [GA]^AATT[CT] BbsI 763 GAAGAC GAAGAC(2/6) BclI 380 TGATCA T^GATCA BfaI 644 CTAG C^TAG BmyI 649 GTGCCC G[AGT]GC[ACT]^C BpmI 630 CTGGAG CTGGAG(16/14) BpuAI 763 GAAGAC GAAGAC(2/6) BseRI 197 CTCCTC GAGGAG(10/8) BsiYI 432 CCAGGGGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 432 CCAGGGGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 649 GTGCCC G[AGT]GC[ACT]^C BspLU11I 880 ACATGT A^CATGT BstUI 453 CGCG CG^CG EcoT22I 849 ATGCAT ATGCA^T FbaI 380 TGATCA T^GATCA MaeI 644 CTAG C^TAG MfeI 602 CAATTG C^AATTG MseI 820 TTAA T^TAA MslI 528 CATTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 602 CAATTG C^AATTG MvnI 453 CGCG CG^CG NsiI 849 ATGCAT ATGCA^T SphI 847 GCATGC GCATG^C Tru9I 820 TTAA T^TAA TspRI 553 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BbvI 355 GCAGC GCAGC(8/12) 595 GCTGC BsmAI 633 GAGAC GTCTC(1/5) 426 GTCTC BsmFI 436 GGGAC GGGAC(10/14) 884 GTCCC BsmI 249 GAATGC GAATGC(1/-1) 542 GAATGC Cfr13I 281 GGCCC G^G[ACGT]CC 306 GGCCC DpnII 153 GATC ^GATC 381 GATC Fnu4HI 355 GCAGC GC^[ACGT]GC 595 GCTGC HaeIII 281 GGCC GG^CC 306 GGCC HgaI 27 GACGC GACGC(5/10) 626 GCGTC ItaI 355 GCAGC GC^[ACGT]GC 595 GCTGC MaeII 318 ACGT A^CGT 350 ACGT MaeIII 517 GTGAC ^GT[ACGT]AC 899 GTGAC MboI 153 GATC ^GATC 381 GATC MboII 696 GAAGA GAAGA(8/7) 763 GAAGA MwoI 598 GCCACAATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 714 GCTTCAACTGC NdeII 153 GATC ^GATC 381 GATC Sau3AI 153 GATC ^GATC 381 GATC Sau96I 281 GGCCC G^G[ACGT]CC 306 GGCCC TseI 355 GCAGC G^C[AT]GC 595 GCTGC Tsp45I 517 GTGAC ^GT[GC]AC 899 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AfaI AluI BsaJI BssKI BstF5I BstNI Cac8I DdeI EcoRII FokI HinfI HphI MnlI MvaI NlaIII NspI PleI RsaI ScrFI TaqI TfiI Tsp509I TthHB8I