RNF130 ORF clone pFN21AE3811

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Halotag ORF Clone pFN21AE3811

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Clone info.

Species

human

Clone Name

pFN21AE3811 Sequence (with vector) Restriction Map

Product ID

FHC11096

ORF Sequence

DNA sequence (1257 bp)
Protein sequence (419 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3811

Symbol

RNF130

Alias : G1RP, G1RZFP, GOLIATH, GP

Description

ring finger protein 130, transcript variant 1

Original Clone ID

cp93811

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1257 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 419 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4451.1 4.7e-160 100.0 RNF130 CCDS64340.1 2.3e-146 99.7 RNF130 CCDS34065.1 1.2e-66 54.1 RNF150 CCDS2051.1 1.4e-46 41.7 RNF149 CCDS14521.1 1.5e-43 44.4 RNF128 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003137 96 169 PF02225 Protease-associated domain IPR018957 264 304 PF00097 Zinc finger HMMSmart IPR001841 264 304 SM00184 Zinc finger ProfileScan IPR001841 264 305 PS50089 Zinc finger
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 3 CAGRAGPARLAALALLTCSLWPA 25 SECONDARY 23 2 163 KDILSYLEKNISVQMTIAVGTRM 185 SECONDARY 23 3 195 LVFVSISFIVLMIISSAWLIFYF 217 PRIMARY 23 4 388 IIASFGLLSALTLCYMIIRATAS 410 PRIMARY 23 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3811 Download

>KIEE3811  1257 bp
ATGAGCTGCGCGGGGCGGGCGGGCCCTGCCCGGCTCGCCGCGCTAGCCCTGCTGACCTGC
AGCCTGTGGCCGGCACGGGCAGACAACGCGAGCCAGGAGTACTACACAGCGCTCATCAAC
GTGACGGTGCAGGAGCCCGGCCGCGGCGCCCCGCTCACGTTTCGCATCGACCGCGGGCGC
TACGGGCTTGACTCCCCCAAGGCCGAGGTCCGCGGCCAGGTGCTGGCGCCGCTGCCCCTC
CACGGAGTTGCTGATCATCTGGGCTGTGATCCACAAACCCGGTTCTTTGTCCCTCCTAAT
ATCAAACAGTGGATTGCCTTGCTGCAGAGGGGAAACTGCACGTTTAAAGAGAAAATATCA
CGGGCCGCTTTCCACAATGCAGTTGCTGTAGTCATCTACAATAATAAATCCAAAGAGGAG
CCAGTTACCATGACTCATCCAGGCACTGGAGATATTATTGCTGTCATGATAACAGAATTG
AGGGGTAAGGATATTTTGAGTTATCTGGAGAAAAACATCTCTGTACAAATGACAATAGCT
GTTGGAACTCGAATGCCACCGAAGAACTTCAGCCGTGGCTCTCTAGTCTTCGTGTCAATA
TCCTTTATTGTTTTGATGATTATTTCTTCAGCATGGCTCATATTCTACTTCATTCAGAAG
ATCAGGTACACAAATGCACGCGACAGGAACCAGCGTCGTCTCGGAGATGCAGCCAAGAAA
GCCATCAGTAAATTGACAACCAGGACAGTAAAGAAGGGTGACAAGGAAACTGACCCAGAC
TTTGATCATTGTGCAGTCTGCATAGAGAGCTATAAGCAGAATGATGTCGTCCGAATTCTC
CCCTGCAAGCATGTTTTCCACAAATCCTGCGTGGATCCCTGGCTTAGTGAACATTGTACC
TGTCCTATGTGCAAACTTAATATATTGAAGGCCCTGGGAATTGTGCCGAATTTGCCATGT
ACTGATAACGTAGCATTCGATATGGAAAGGCTCACCAGAACCCAAGCTGTTAACCGAAGA
TCAGCCCTCGGCGACCTCGCCGGCGACAACTCCCTTGGCCTTGAGCCACTTCGAACTTCG
GGGATCTCACCTCTTCCTCAGGATGGGGAGCTCACTCCGAGAACAGGAGAAATCAACATT
GCAGTAACAAAAGAATGGTTTATTATTGCCAGTTTTGGCCTCCTCAGTGCCCTCACACTC
TGCTACATGATCATCAGAGCCACAGCTAGCTTGAATGCTAATGAGGTAGAATGGTTT

ORF Protein

Cloned ORF protein sequence for pFN21AE3811 Download

>KIEE3811  419 aa
MSCAGRAGPARLAALALLTCSLWPARADNASQEYYTALINVTVQEPGRGAPLTFRIDRGR
YGLDSPKAEVRGQVLAPLPLHGVADHLGCDPQTRFFVPPNIKQWIALLQRGNCTFKEKIS
RAAFHNAVAVVIYNNKSKEEPVTMTHPGTGDIIAVMITELRGKDILSYLEKNISVQMTIA
VGTRMPPKNFSRGSLVFVSISFIVLMIISSAWLIFYFIQKIRYTNARDRNQRRLGDAAKK
AISKLTTRTVKKGDKETDPDFDHCAVCIESYKQNDVVRILPCKHVFHKSCVDPWLSEHCT
CPMCKLNILKALGIVPNLPCTDNVAFDMERLTRTQAVNRRSALGDLAGDNSLGLEPLRTS
GISPLPQDGELTPRTGEINIAVTKEWFIIASFGLLSALTLCYMIIRATASLNANEVEWF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3811 Download

>pFN21AE3811  5962 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGCTGCGCGGGGCGGGCGGGCCCTGCCCGGCT
CGCCGCGCTAGCCCTGCTGACCTGCAGCCTGTGGCCGGCACGGGCAGACAACGCGAGCCA
GGAGTACTACACAGCGCTCATCAACGTGACGGTGCAGGAGCCCGGCCGCGGCGCCCCGCT
CACGTTTCGCATCGACCGCGGGCGCTACGGGCTTGACTCCCCCAAGGCCGAGGTCCGCGG
CCAGGTGCTGGCGCCGCTGCCCCTCCACGGAGTTGCTGATCATCTGGGCTGTGATCCACA
AACCCGGTTCTTTGTCCCTCCTAATATCAAACAGTGGATTGCCTTGCTGCAGAGGGGAAA
CTGCACGTTTAAAGAGAAAATATCACGGGCCGCTTTCCACAATGCAGTTGCTGTAGTCAT
CTACAATAATAAATCCAAAGAGGAGCCAGTTACCATGACTCATCCAGGCACTGGAGATAT
TATTGCTGTCATGATAACAGAATTGAGGGGTAAGGATATTTTGAGTTATCTGGAGAAAAA
CATCTCTGTACAAATGACAATAGCTGTTGGAACTCGAATGCCACCGAAGAACTTCAGCCG
TGGCTCTCTAGTCTTCGTGTCAATATCCTTTATTGTTTTGATGATTATTTCTTCAGCATG
GCTCATATTCTACTTCATTCAGAAGATCAGGTACACAAATGCACGCGACAGGAACCAGCG
TCGTCTCGGAGATGCAGCCAAGAAAGCCATCAGTAAATTGACAACCAGGACAGTAAAGAA
GGGTGACAAGGAAACTGACCCAGACTTTGATCATTGTGCAGTCTGCATAGAGAGCTATAA
GCAGAATGATGTCGTCCGAATTCTCCCCTGCAAGCATGTTTTCCACAAATCCTGCGTGGA
TCCCTGGCTTAGTGAACATTGTACCTGTCCTATGTGCAAACTTAATATATTGAAGGCCCT
GGGAATTGTGCCGAATTTGCCATGTACTGATAACGTAGCATTCGATATGGAAAGGCTCAC
CAGAACCCAAGCTGTTAACCGAAGATCAGCCCTCGGCGACCTCGCCGGCGACAACTCCCT
TGGCCTTGAGCCACTTCGAACTTCGGGGATCTCACCTCTTCCTCAGGATGGGGAGCTCAC
TCCGAGAACAGGAGAAATCAACATTGCAGTAACAAAAGAATGGTTTATTATTGCCAGTTT
TGGCCTCCTCAGTGCCCTCACACTCTGCTACATGATCATCAGAGCCACAGCTAGCTTGAA
TGCTAATGAGGTAGAATGGTTTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT