(Circular) (Six-base) MAPSORT of: pFN21AE3811.seq Check: 247 from: 1 to: 5962 pFN21AE3811 5962 bp With 182 enzymes: SgfI * March 28, 2012 12:12 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3772 278 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3305 1937 Size: 1368 4594 Acc65I G'GTAC_C Cuts at: 3283 3283 Size: 5962 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2557 2616 4025 5409 5677 1632 Size: 925 59 1409 1384 268 1917 Fragments arranged by size: 1917 1409 1384 925 268 59 AfeI AGC'GCT Cuts at: 2115 2115 Size: 5962 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 4861 5838 1203 Size: 3658 977 1327 Fragments arranged by size: 3658 1327 977 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 4691 4691 Size: 5962 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5962 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5962 AlwNI CAG_nnn'CTG Cuts at: 2227 2390 2450 3705 5277 2227 Size: 163 60 1255 1572 2912 Fragments arranged by size: 2912 1572 1255 163 60 ApaI G_GGCC'C Cuts at: 2030 2030 Size: 5962 ApaLI G'TGCA_C Cuts at: 4019 5175 4019 Size: 1156 4806 ApoI r'AATT_y Cuts at: 1493 2838 2953 3271 3497 3649 5758 1493 Size: 1345 115 318 226 152 2109 1697 Fragments arranged by size: 2109 1697 1345 318 226 152 115 AseI AT'TA_AT Cuts at: 160 1032 4516 160 Size: 872 3484 1606 Fragments arranged by size: 3484 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 3287 1870 Size: 73 1344 4545 Fragments arranged by size: 4545 1344 73 BamHI G'GATC_C Cuts at: 2878 3292 3659 2878 Size: 414 367 5181 Fragments arranged by size: 5181 414 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2150 2230 3283 4738 618 Size: 325 217 627 363 80 1053 1455 1842 Fragments arranged by size: 1842 1455 1053 627 363 325 217 80 BanII G_rGCy'C Cuts at: 1391 2030 2142 3117 3281 1391 Size: 639 112 975 164 4072 Fragments arranged by size: 4072 975 639 164 112 BbeI G_GCGC'C Cuts at: 2154 2234 2154 Size: 80 5882 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2584 928 Size: 971 685 4306 Fragments arranged by size: 4306 971 685 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4153 4187 1903 Size: 34 2216 34 3678 Fragments arranged by size: 3678 2216 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3854 5064 3854 Size: 1210 4752 BclI T'GATC_A Cuts at: 1548 2257 2788 3213 1548 Size: 709 531 425 4297 Fragments arranged by size: 4297 709 531 425 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4573 136 Size: 107 122 71 631 605 2901 1525 Fragments arranged by size: 2901 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5957 5957 Size: 5962 BlpI GC'TnA_GC Cuts at: 3379 3379 Size: 5962 Bme1580I G_kGCm'C Cuts at: 1790 2030 3196 4023 5179 1790 Size: 240 1166 827 1156 2573 Fragments arranged by size: 2573 1166 1156 827 240 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5962 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3693 4651 442 Size: 416 53 83 399 24 2276 958 1753 Fragments arranged by size: 2276 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2051 3234 1056 Size: 995 1183 3784 Fragments arranged by size: 3784 1183 995 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2472 2531 4622 1239 Size: 1233 59 2091 2579 Fragments arranged by size: 2579 2091 1233 59 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3086 4087 4952 5250 3086 Size: 1001 865 298 3798 Fragments arranged by size: 3798 1001 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4625 882 Size: 617 3126 2219 Fragments arranged by size: 3126 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3753 493 Size: 711 2549 2702 Fragments arranged by size: 2702 2549 711 BsaBI GATnn'nnATC Cuts at: 5956 5956 Size: 5962 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2151 2231 3769 4151 Size: 53 83 186 936 618 80 1538 382 Cuts at: 4151 4786 275 Size: 635 1451 Fragments arranged by size: 1538 1451 936 635 618 382 186 83 80 53 BsaWI w'CCGG_w Cuts at: 4395 4793 5067 5214 5625 4395 Size: 398 274 147 411 4732 Fragments arranged by size: 4732 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5737 Fragments arranged by size: 5737 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2435 3176 1186 Size: 1249 741 3972 Fragments arranged by size: 3972 1249 741 BseYI C'CCAG_C Cuts at: 5165 5661 5165 Size: 496 5466 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 2326 2817 1163 Size: 573 417 173 491 4308 Fragments arranged by size: 4308 573 491 417 173 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2146 2176 3412 4173 5201 1540 Size: 275 186 145 30 1236 761 1028 2301 Fragments arranged by size: 2301 1236 1028 761 275 186 145 30 BsiHKAI G_wGCw'C Cuts at: 3117 4023 4108 5179 3117 Size: 906 85 1071 3900 Fragments arranged by size: 3900 1071 906 85 BsmI GAATG_Cn' Cuts at: 2562 2978 3244 3473 3566 2562 Size: 416 266 229 93 4958 Fragments arranged by size: 4958 416 266 229 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2708 2708 Size: 5962 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2142 3117 3196 3281 4023 4108 Size: 399 240 112 975 79 85 742 85 Cuts at: 4108 5179 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 975 742 399 240 112 85 85 79 BspEI T'CCGG_A Cuts at: 5625 5625 Size: 5962 BspHI T'CATG_A Cuts at: 2469 3851 5581 2469 Size: 1382 1730 2850 Fragments arranged by size: 2850 1730 1382 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2069 3317 844 Size: 1225 1248 3489 Fragments arranged by size: 3489 1248 1225 BsrBI CCG'CTC Cuts at: 1394 1823 2158 3849 1394 Size: 429 335 1691 3507 Fragments arranged by size: 3507 1691 429 335 BsrDI GCAATG_nn' Cuts at: 65 1248 3141 4457 4631 65 Size: 1183 1893 1316 174 1396 Fragments arranged by size: 1893 1396 1316 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2074 3044 4606 4793 1700 Size: 110 264 970 1562 187 2869 Fragments arranged by size: 2869 1562 970 264 187 110 BsrGI T'GTAC_A Cuts at: 96 2527 96 Size: 2431 3531 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5962 BssSI C'ACGA_G Cuts at: 4022 5034 4022 Size: 1012 4950 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5962 BstBI TT'CG_AA Cuts at: 3076 4766 3076 Size: 1690 4272 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5962 BstYI r'GATC_y Cuts at: 1972 2878 3087 3292 3659 4044 4061 5513 5599 Size: 906 209 205 367 385 17 1452 86 Cuts at: 5599 5611 5957 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 906 385 367 346 209 205 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3102 3102 Size: 5962 BtgI C'CryG_G Cuts at: 513 1065 2146 2176 2215 2245 2578 513 Size: 552 1081 30 39 30 333 3897 Fragments arranged by size: 3897 1081 552 333 39 30 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5223 Fragments arranged by size: 5223 683 56 BtsI GCAGTG_nn' Cuts at: 3478 4272 4292 3478 Size: 794 20 5148 Fragments arranged by size: 5148 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5962 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5927 DraI TTT'AAA Cuts at: 2350 3266 2350 Size: 916 5046 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4969 809 Size: 507 3653 1802 Fragments arranged by size: 3653 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2072 2143 2218 Size: 54 1425 50 275 18 242 71 75 Cuts at: 2218 3409 4298 5927 8 Size: 1191 889 1629 43 Fragments arranged by size: 1629 1425 1191 889 275 242 75 71 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2143 3409 1537 Size: 275 331 1266 4090 Fragments arranged by size: 4090 1266 331 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3102 3892 1353 Size: 1749 790 3423 Fragments arranged by size: 3423 1749 790 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4549 4923 5069 387 Size: 1064 138 2960 374 146 1280 Fragments arranged by size: 2960 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3115 3115 Size: 5962 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2472 2531 2557 2616 4025 4622 5409 Size: 393 840 59 26 59 1409 597 787 Cuts at: 5409 5677 1239 Size: 268 1524 Fragments arranged by size: 1524 1409 840 787 597 393 268 59 59 26 EcoO109I rG'GnC_Cy Cuts at: 2027 2935 2027 Size: 908 5054 EcoRI G'AATT_C Cuts at: 2838 3271 3649 2838 Size: 433 378 5151 Fragments arranged by size: 5151 433 378 FspI TGC'GCA Cuts at: 4468 4468 Size: 5962 HaeII r_GCGC'y Cuts at: 1678 2117 2154 2185 2234 5109 1678 Size: 439 37 31 49 2875 2531 Fragments arranged by size: 2875 2531 439 49 37 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 3015 3047 3079 Size: 32 135 32 773 32 638 32 32 Cuts at: 3079 3111 4603 4635 4677 4709 1373 Size: 32 1492 32 42 32 2626 Fragments arranged by size: 2626 1492 773 638 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3016 3306 3552 669 Size: 988 281 1078 290 246 3079 Fragments arranged by size: 3079 1078 988 290 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5962 HpaI GTT'AAC Cuts at: 3016 3552 3016 Size: 536 5426 KasI G'GCGC_C Cuts at: 2150 2230 2150 Size: 80 5882 KpnI G_GTAC'C Cuts at: 3287 3287 Size: 5962 MfeI C'AATT_G Cuts at: 3561 3561 Size: 5962 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2527 5076 5260 1305 Size: 1222 2549 184 2007 Fragments arranged by size: 2549 2007 1222 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4140 Fragments arranged by size: 4140 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2123 3920 4279 4438 518 Size: 648 957 1797 359 159 2042 Fragments arranged by size: 2042 1797 957 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2148 2178 2217 2236 3378 4055 5203 5448 Size: 542 30 39 19 1142 677 1148 245 Cuts at: 5448 1606 Size: 2120 Fragments arranged by size: 2120 1148 1142 677 542 245 39 30 19 NaeI GCC'GGC Cuts at: 2076 3046 2076 Size: 970 4992 NarI GG'CG_CC Cuts at: 2151 2231 2151 Size: 80 5882 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5410 NdeI CA'TA_TG Cuts at: 387 387 Size: 5962 NgoMIV G'CCGG_C Cuts at: 2074 3044 2074 Size: 970 4992 NheI G'CTAG_C Cuts at: 1052 2047 3230 1052 Size: 995 1183 3784 Fragments arranged by size: 3784 1183 995 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NspI r_CATG'y Cuts at: 1132 1229 2858 3320 4865 1132 Size: 97 1629 462 1545 2229 Fragments arranged by size: 2229 1629 1545 462 97 PasI CC'CwG_GG Cuts at: 1348 2938 1348 Size: 1590 4372 PciI A'CATG_T Cuts at: 4861 4861 Size: 5962 PflMI CCAn_nnn'nTGG Cuts at: 1108 2227 2450 1108 Size: 1119 223 4620 Fragments arranged by size: 4620 1119 223 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4031 4063 5544 5576 4031 Size: 32 1481 32 4417 Fragments arranged by size: 4417 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5962 PsiI TTA'TAA Cuts at: 3532 3532 Size: 5962 PspOMI G'GGCC_C Cuts at: 2026 2026 Size: 5962 PstI C_TGCA'G Cuts at: 830 2066 2331 3314 830 Size: 1236 265 983 3478 Fragments arranged by size: 3478 1236 983 265 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 SacI G_AGCT'C Cuts at: 3117 3117 Size: 5962 SacII CC_GC'GG Cuts at: 2149 2179 2218 2149 Size: 30 39 5893 Fragments arranged by size: 5893 39 30 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 2105 4210 5922 2105 Size: 2105 1712 2145 Fragments arranged by size: 2145 2105 1712 SfcI C'TryA_G Cuts at: 826 1045 2062 2327 2391 3310 4445 5126 5317 Size: 219 1017 265 64 919 1135 681 191 Cuts at: 5317 826 Size: 1471 Fragments arranged by size: 1471 1135 1017 919 681 265 219 191 64 SfoI GGC'GCC Cuts at: 2152 2232 2152 Size: 80 5882 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 3044 1700 Size: 1344 4618 SmaI CCC'GGG Cuts at: 1872 3289 1872 Size: 1417 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3065 4066 4967 5229 820 Size: 197 926 1122 1001 901 262 1553 Fragments arranged by size: 1553 1122 1001 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 3320 1132 Size: 2188 3774 SspI AAT'ATT Cuts at: 5 52 3886 5 Size: 47 3834 2081 Fragments arranged by size: 3834 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5962 StyI C'CwwG_G Cuts at: 513 1065 2202 3058 3401 513 Size: 552 1137 856 343 3074 Fragments arranged by size: 3074 1137 856 552 343 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3989 4006 4159 4344 4789 325 Size: 1255 2409 17 153 185 445 1498 Fragments arranged by size: 2409 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2103 2527 2963 Size: 274 80 33 51 1443 126 424 436 Cuts at: 2963 4208 5920 96 Size: 1245 1712 138 Fragments arranged by size: 1712 1443 1245 436 424 274 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5922 5954 5922 Size: 32 5930 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5962 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 3287 1870 Size: 1417 4545 XmnI GAAnn'nnTTC Cuts at: 2570 4091 2570 Size: 1521 4441 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3770 276 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI