Restriction map for
pFN21AE3811
##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BsoBI BspDI BspEI
BspLU11I BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraIII DrdI Eam1105I
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HindIII KpnI MamI MfeI
MluI MluNI MroI MscI MunI
NcoI NdeI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SalI
SapI SbfI SexAI SfiI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AocI 1096 CCTCAGG CC^T[ACGT]AGG
Aor51HI 108 AGCGCT AGC^GCT
ApaI 21 GGGCCC GGGCC^C
Asp700I 561 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1108 GAGCTC G[AT]GC[AT]^C
AvaII 207 GGTCC G^G[AT]CC
BamHI 873 GGATCC G^GATCC
BbsI 586 GTCTTC GAAGAC(2/6)
BpuAI 586 GTCTTC GAAGAC(2/6)
BsiHKAI 1108 GAGCTC G[AT]GC[AT]^C
BsmAI 698 GTCTC GTCTC(1/5)
BsmBI 697 CGTCTC CGTCTC(1/5)
BsmFI 289 GTCCC GGGAC(10/14)
Bsp1407I 522 TGTACA T^GTACA
BspHI 464 TCATGA T^CATGA
BspMI 55 ACCTGC ACCTGC(4/8)
BsrBI 151 CCGCTC CCGCTC(-3/-3)
BsrDI 1137 CATTGC GCAATG(2/0)
BsrGI 522 TGTACA T^GTACA
BstBI 1070 TTCGAA TT^CGAA
Bsu36I 1096 CCTCAGG CC^T[ACGT]AGG
DraI 343 TTTAAA TTT^AAA
EagI 138 CGGCCG C^GGCCG
EarI 1091 CTCTTC CTCTTC(1/4)
EclXI 138 CGGCCG C^GGCCG
Eco47III 108 AGCGCT AGC^GCT
Eco52I 138 CGGCCG C^GGCCG
Eco81I 1096 CCTCAGG CC^T[ACGT]AGG
EcoRI 833 GAATTC G^AATTC
HgaI 693 GCGTC GACGC(5/10)
HincII 1009 GTTAAC GT[CT]^[GA]AC
HindII 1009 GTTAAC GT[CT]^[GA]AC
HpaI 1009 GTTAAC GTT^AAC
Ksp632I 1091 CTCTTC CTCTTC(1/4)
MslI 114 CATCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 849 GCATGT [GA]CATG^[CT]
NspV 1070 TTCGAA TT^CGAA
RcaI 464 TCATGA T^CATGA
SacI 1108 GAGCTC GAGCT^C
ScaI 98 AGTACT AGT^ACT
SfuI 1070 TTCGAA TT^CGAA
SgrAI 1038 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1060 CTTGAG C^T[CT][GA]AG
SspBI 522 TGTACA T^GTACA
XmnI 561 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 833 GAATTC [GA]^AATT[CT]
948 GAATTT
AcyI 145 GGCGCC G[GA]^CG[CT]C
225 GGCGCC
ApoI 833 GAATTC [GA]^AATT[CT]
948 GAATTT
BanI 145 GGCGCC G^G[CT][GA]CC
225 GGCGCC
BbeI 145 GGCGCC GGCGC^C
225 GGCGCC
BpmI 446 CTGGAG CTGGAG(16/14)
505 CTGGAG
BsaHI 145 GGCGCC G[GA]^CG[CT]C
225 GGCGCC
BseRI 1180 CTCCTC GAGGAG(10/8)
415 GAGGAG
BsiEI 138 CGGCCG CG[GA][CT]^CG
168 CGACCG
BsrFI 69 GCCGGC [GA]^CCGG[CT]
1039 GCCGGC
BstF5I 436 CATCC GGATG(2/0)
1101 GGATG
BstYI 873 GGATCC [GA]^GATC[CT]
1082 GGATCT
Cfr10I 69 GCCGGC [GA]^CCGG[CT]
1039 GCCGGC
DraII 21 GGGCCCT [GA]G^G[ACGT]CC[CT]
929 AGGCCCT
EcoO109I 21 GGGCCCT [GA]G^G[ACGT]CC[CT]
929 AGGCCCT
EcoT14I 197 CCAAGG C^C[AT][AT]GG
1053 CCTTGG
FokI 436 CATCC GGATG(9/13)
1101 GGATG
Hin1I 145 GGCGCC G[GA]^CG[CT]C
225 GGCGCC
HinfI 190 GACTC G^A[ACGT]TC
432 GACTC
KasI 145 GGCGCC G^GCGCC
225 GGCGCC
MflI 873 GGATCC [GA]^GATC[CT]
1082 GGATCT
NaeI 69 GCCGGC GCC^GGC
1039 GCCGGC
NarI 145 GGCGCC GG^CGCC
225 GGCGCC
NgoMIV 69 GCCGGC G^CCGGC
1039 GCCGGC
NheI 42 GCTAGC G^CTAGC
1225 GCTAGC
PflMI 216 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
439 CCAGGCACTGG
PleI 190 GACTC GAGTC(4/5)
432 GACTC
PstI 57 CTGCAG CTGCA^G
322 CTGCAG
SfaNI 706 GATGC GCATC(5/9)
164 GCATC
SfoI 145 GGCGCC GGC^GCC
225 GGCGCC
StyI 197 CCAAGG C^C[AT][AT]GG
1053 CCTTGG
Tsp45I 121 GTGAC ^GT[GC]AC
758 GTGAC
Van91I 216 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
439 CCAGGCACTGG
XhoII 873 GGATCC [GA]^GATC[CT]
1082 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI BanII BbvI BclI BcnI
BfaI BmyI BsaJI BsgI BsiYI
BslI BsmI Bsp1286I BsrI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoRII
FauI FbaI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI KspI MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV RsaI SacII
Sau3AI Sau96I ScrFI SfcI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I