ARHGAP27 ORF clone pFN21AE3557

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Halotag ORF Clone pFN21AE3557

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Clone info.

Species

human

Clone Name

pFN21AE3557 Sequence (with vector) Restriction Map

Product ID

FHC12581

Flexi Clone PID

FXC12581

ORF Sequence

DNA sequence (1644 bp)
Protein sequence (548 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3557

Symbol

ARHGAP27

Alias : CAMGAP1, PP905, SH3D20, SH3P20

Description

Rho GTPase activating protein 27, transcript variant 1

Original Clone ID

cp93557

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1644 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 548 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11498.1 2.4e-152 100.0 ARHGAP27 CCDS74082.1 3.4e-152 100.0 ARHGAP27 CCDS59216.1 1.1e-54 44.6 ARHGAP12 CCDS59214.1 2.1e-52 43.2 ARHGAP12 CCDS73082.1 5.2e-49 43.0 ARHGAP12 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001849 158 268 PF00169 Pleckstrin homology domain IPR000198 370 519 PF00620 Rho GTPase-activating protein domain HMMSmart IPR001202 71 103 SM00456 WW/Rsp5/WWP IPR001849 156 273 SM00233 Pleckstrin homology domain IPR000198 367 542 SM00324 Rho GTPase-activating protein domain ProfileScan IPR001202 76 103 PS50020 WW/Rsp5/WWP IPR001849 155 271 PS50003 Pleckstrin homology domain IPR000198 356 545 PS50238 Rho GTPase-activating protein domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_954976 548 610591 100.0 100.0 XP_016879801 689 610591 100.0 100.0 NP_001269219 889 610591 100.0 100.0 XP_006721808 889 610591 100.0 100.0 XP_005257185 667 610591 96.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3557 Download

>KIEE3557  1644 bp
ATGCAGCCGGGCCTGAGCCCTGGCAGCCCAGGGGACCCGCGGCCCCCCACTCCCGAGACG
GACTACCCCGAGTCGCTGACCAGTTACCCCGAGGAGGACTATTCTCCCGTGGGCTCTTTC
GGTGAGCCCGGCCCTACCTCTCCCTTGACCACACCCCCCGGCTGGTCTTGTCATGTCAGC
CAGGACAAGCAGATGCTCTACACCAACCACTTCACTCAGGAGCAGTGGGTGAGGCTGGAG
GACCCCCACGGGAAGCCATACTTCTACAATCCAGAGGACTCCTCTGTTCGATGGGAGCTG
CCCCAGGTCCCTGTCCCTGCCCCTCGAAGCATCCATAAATCCAGCCAGGATGGTGACACC
CCAGCCCAGGCCAGCCCTCCAGAGGAGAAGGTCCCAGCAGAGCTGGATGAGGTTGGGAGC
TGGGAGGAAGTCTCTCCTGCCACAGCTGCTGTGAGGACCAAGACCTTGGACAAGGCAGGG
GTGCTCCATCGCACCAAGACGGCAGACAAGGGAAAGCGGCTCCGGAAGAAGCACTGGAGT
GCCTCCTGGACTGTGCTGGAGGGTGGCGTCCTGACATTCTTCAAGGACTCAAAGACCTCG
GCTGCAGGCGGCCTGAGGCAGCCTTCCAAGTTTTCCACCCCTGAGTACACAGTGGAGCTG
AGGGGGGCCACTCTCTCCTGGGCCCCCAAAGACAAATCCAGTAGGAAGAATGTGCTGGAG
CTACGGAGCCGAGATGGCTCTGAGTACCTGATCCAGCACGACTCGGAGGCCATCATCAGC
ACCTGGCATAAGGCCATTGCTCAGGGCATCCAGGAGCTGTCCGCAGAGCTGCCCCCAGAG
GAGAGCGAGAGCAGCAGAGTGGACTTCGGGTCGAGCGAGCGCTTGGGAAGCTGGCAGGAG
AAAGAGGAGGACGCGCGACCGAATGCAGCCGCGCCCGCCCTGGGCCCCGTGGGCCTGGAG
AGCGACTTGAGCAAGGTCCGGCACAAGCTCCGCAAGTTCCTCCAGAGGCGGCCCACACTG
CAGTCGCTGCGGGAGAAGGGCTACATCAAAGACCAGGTGTTCGGCTGCGCGCTGGCCGCG
CTGTGTGAGCGCGAGAGGAGCCGGGTGCCACGCTTCGTGCAGCAGTGCATCCGCGCCGTC
GAGGCCCGCGGGCTGGACATCGACGGGCTGTACCGCATCAGTGGAAACCTGGCCACCATC
CAGAAGCTACGCTATAAGGTGGACCACGATGAGCGCCTTGACCTGGATGACGGGCGCTGG
GAGGACGTCCACGTTATCACCGGAGCCCTGAAGCTCTTCTTTCGGGAGCTGCCCGAGCCC
CTCTTCCCCTTCTCGCACTTCCGCCAGTTCATTGCGGCCATCAAGTTGCAGGACCAGGCC
CGGCGCAGCCGCTGTGTGCGTGACTTGGTGCGCTCGCTGCCCGCTCCCAACCACGACACT
CTGCGGATGCTCTTCCAGCACCTCTGCCGGGTGATCGAGCACGGCGAGCAGAACCGCATG
TCGGTGCAGAGCGTGGCCATTGTGTTCGGGCCCACGCTGCTGCGGCCCGAGGTGGAAGAG
ACCAGCATGCCCATGACCATGGTGTTCCAGAACCAGGTGGTGGAGCTCATCCTGCAGCAG
TGCGCGGACATCTTCCCGCCGCAC

ORF Protein

Cloned ORF protein sequence for pFN21AE3557 Download

>KIEE3557  548 aa
MQPGLSPGSPGDPRPPTPETDYPESLTSYPEEDYSPVGSFGEPGPTSPLTTPPGWSCHVS
QDKQMLYTNHFTQEQWVRLEDPHGKPYFYNPEDSSVRWELPQVPVPAPRSIHKSSQDGDT
PAQASPPEEKVPAELDEVGSWEEVSPATAAVRTKTLDKAGVLHRTKTADKGKRLRKKHWS
ASWTVLEGGVLTFFKDSKTSAAGGLRQPSKFSTPEYTVELRGATLSWAPKDKSSRKNVLE
LRSRDGSEYLIQHDSEAIISTWHKAIAQGIQELSAELPPEESESSRVDFGSSERLGSWQE
KEEDARPNAAAPALGPVGLESDLSKVRHKLRKFLQRRPTLQSLREKGYIKDQVFGCALAA
LCERERSRVPRFVQQCIRAVEARGLDIDGLYRISGNLATIQKLRYKVDHDERLDLDDGRW
EDVHVITGALKLFFRELPEPLFPFSHFRQFIAAIKLQDQARRSRCVRDLVRSLPAPNHDT
LRMLFQHLCRVIEHGEQNRMSVQSVAIVFGPTLLRPEVEETSMPMTMVFQNQVVELILQQ
CADIFPPH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3557 Download

>pFN21AE3557  6349 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCAGCCGGGCCTGAGCCCTGGCAGCCCAGGGGA
CCCGCGGCCCCCCACTCCCGAGACGGACTACCCCGAGTCGCTGACCAGTTACCCCGAGGA
GGACTATTCTCCCGTGGGCTCTTTCGGTGAGCCCGGCCCTACCTCTCCCTTGACCACACC
CCCCGGCTGGTCTTGTCATGTCAGCCAGGACAAGCAGATGCTCTACACCAACCACTTCAC
TCAGGAGCAGTGGGTGAGGCTGGAGGACCCCCACGGGAAGCCATACTTCTACAATCCAGA
GGACTCCTCTGTTCGATGGGAGCTGCCCCAGGTCCCTGTCCCTGCCCCTCGAAGCATCCA
TAAATCCAGCCAGGATGGTGACACCCCAGCCCAGGCCAGCCCTCCAGAGGAGAAGGTCCC
AGCAGAGCTGGATGAGGTTGGGAGCTGGGAGGAAGTCTCTCCTGCCACAGCTGCTGTGAG
GACCAAGACCTTGGACAAGGCAGGGGTGCTCCATCGCACCAAGACGGCAGACAAGGGAAA
GCGGCTCCGGAAGAAGCACTGGAGTGCCTCCTGGACTGTGCTGGAGGGTGGCGTCCTGAC
ATTCTTCAAGGACTCAAAGACCTCGGCTGCAGGCGGCCTGAGGCAGCCTTCCAAGTTTTC
CACCCCTGAGTACACAGTGGAGCTGAGGGGGGCCACTCTCTCCTGGGCCCCCAAAGACAA
ATCCAGTAGGAAGAATGTGCTGGAGCTACGGAGCCGAGATGGCTCTGAGTACCTGATCCA
GCACGACTCGGAGGCCATCATCAGCACCTGGCATAAGGCCATTGCTCAGGGCATCCAGGA
GCTGTCCGCAGAGCTGCCCCCAGAGGAGAGCGAGAGCAGCAGAGTGGACTTCGGGTCGAG
CGAGCGCTTGGGAAGCTGGCAGGAGAAAGAGGAGGACGCGCGACCGAATGCAGCCGCGCC
CGCCCTGGGCCCCGTGGGCCTGGAGAGCGACTTGAGCAAGGTCCGGCACAAGCTCCGCAA
GTTCCTCCAGAGGCGGCCCACACTGCAGTCGCTGCGGGAGAAGGGCTACATCAAAGACCA
GGTGTTCGGCTGCGCGCTGGCCGCGCTGTGTGAGCGCGAGAGGAGCCGGGTGCCACGCTT
CGTGCAGCAGTGCATCCGCGCCGTCGAGGCCCGCGGGCTGGACATCGACGGGCTGTACCG
CATCAGTGGAAACCTGGCCACCATCCAGAAGCTACGCTATAAGGTGGACCACGATGAGCG
CCTTGACCTGGATGACGGGCGCTGGGAGGACGTCCACGTTATCACCGGAGCCCTGAAGCT
CTTCTTTCGGGAGCTGCCCGAGCCCCTCTTCCCCTTCTCGCACTTCCGCCAGTTCATTGC
GGCCATCAAGTTGCAGGACCAGGCCCGGCGCAGCCGCTGTGTGCGTGACTTGGTGCGCTC
GCTGCCCGCTCCCAACCACGACACTCTGCGGATGCTCTTCCAGCACCTCTGCCGGGTGAT
CGAGCACGGCGAGCAGAACCGCATGTCGGTGCAGAGCGTGGCCATTGTGTTCGGGCCCAC
GCTGCTGCGGCCCGAGGTGGAAGAGACCAGCATGCCCATGACCATGGTGTTCCAGAACCA
GGTGGTGGAGCTCATCCTGCAGCAGTGCGCGGACATCTTCCCGCCGCACGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT