(Circular) (Six-base) MAPSORT of: pFN21AE3557.seq Check: 6442 from: 1 to: 6349 pFN21AE3557 6349 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3273 4159 278 Size: 53 83 186 936 1737 886 2468 Fragments arranged by size: 2468 1737 936 886 186 83 53 AccI GT'mk_AC Cuts at: 1937 3692 1937 Size: 1755 4594 Acc65I G'GTAC_C Cuts at: 3670 3670 Size: 6349 AclI AA'CG_TT Cuts at: 1563 4476 4849 1563 Size: 2913 373 3063 Fragments arranged by size: 3063 2913 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3314 4412 5796 6064 1632 Size: 1682 1098 1384 268 1917 Fragments arranged by size: 1917 1682 1384 1098 268 AfeI AGC'GCT Cuts at: 2885 2885 Size: 6349 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6152 AflIII A'CryG_T Cuts at: 1203 5248 6225 1203 Size: 4045 977 1327 Fragments arranged by size: 4045 1327 977 AgeI A'CCGG_T Cuts at: 5180 5180 Size: 6349 AhdI GACnn_n'nnGTC Cuts at: 2872 5078 2872 Size: 2206 4143 AleI CACnn'nnGTG Cuts at: 1166 2541 1166 Size: 1375 4974 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2559 3524 1184 Size: 1375 965 4009 Fragments arranged by size: 4009 1375 965 AlwNI CAG_nnn'CTG Cuts at: 2453 2787 2821 3031 3396 4092 5664 2453 Size: 334 34 210 365 696 1572 3138 Fragments arranged by size: 3138 1572 696 365 334 210 34 ApaI G_GGCC'C Cuts at: 2689 2951 3537 2689 Size: 262 586 5501 Fragments arranged by size: 5501 586 262 ApaLI G'TGCA_C Cuts at: 4406 5562 4406 Size: 1156 5193 ApoI r'AATT_y Cuts at: 1493 3658 3884 4036 6145 1493 Size: 2165 226 152 2109 1697 Fragments arranged by size: 2165 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4903 160 Size: 872 3871 1606 Fragments arranged by size: 3871 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 AvaI C'yCGr_G Cuts at: 1870 1943 2057 2072 2093 3317 3551 3674 1870 Size: 73 114 15 21 1224 234 123 4545 Fragments arranged by size: 4545 1224 234 123 114 73 21 15 BamHI G'GATC_C Cuts at: 3679 4046 3679 Size: 367 5982 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3109 3670 5125 618 Size: 325 217 627 1322 561 1455 1842 Fragments arranged by size: 1842 1455 1322 627 561 325 217 BanII G_rGCy'C Cuts at: 1391 2024 2120 2133 2689 2951 3292 3324 3537 Size: 633 96 13 556 262 341 32 213 Cuts at: 3537 3612 3668 1391 Size: 75 56 4072 Fragments arranged by size: 4072 633 556 341 262 213 96 75 56 32 13 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5378 BbvCI CC'TCA_GC Cuts at: 2663 2663 Size: 6349 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4540 4574 1903 Size: 34 50 34 2519 34 3678 Fragments arranged by size: 3678 2519 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4241 5451 4241 Size: 1210 5139 BclI T'GATC_A Cuts at: 1548 1548 Size: 6349 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2739 2955 3146 Size: 107 122 71 631 605 1067 216 191 Cuts at: 3146 4960 136 Size: 1814 1525 Fragments arranged by size: 1814 1525 1067 631 605 216 191 122 107 71 BglII A'GATC_T Cuts at: 6344 6344 Size: 6349 BlpI GC'TnA_GC Cuts at: 3766 3766 Size: 6349 Bme1580I G_kGCm'C Cuts at: 1790 2689 2951 3537 4410 5566 1790 Size: 899 262 586 873 1156 2573 Fragments arranged by size: 2573 1156 899 873 586 262 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6349 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4080 5038 442 Size: 416 53 83 399 24 2663 958 1753 Fragments arranged by size: 2663 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6349 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2302 2452 2302 Size: 150 6199 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2261 2367 2560 2582 2741 2981 2990 5009 Size: 1022 106 193 22 159 240 9 2019 Cuts at: 5009 1239 Size: 2579 Fragments arranged by size: 2579 2019 1022 240 193 159 106 22 9 Bpu10I CC'TnA_GC Cuts at: 2018 2663 2805 2018 Size: 645 142 5562 Fragments arranged by size: 5562 645 142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2992 4474 5339 5637 2992 Size: 1482 865 298 3704 Fragments arranged by size: 3704 1482 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3557 5012 882 Size: 617 2058 1455 2219 Fragments arranged by size: 2219 2058 1455 617 BsaAI yAC'GTr Cuts at: 493 1204 4140 493 Size: 711 2936 2702 Fragments arranged by size: 2936 2702 711 BsaBI GATnn'nnATC Cuts at: 6343 6343 Size: 6349 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2571 3270 4156 4538 Size: 53 83 186 936 1038 699 886 382 Cuts at: 4538 5173 275 Size: 635 1451 Fragments arranged by size: 1451 1038 936 886 699 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2526 3284 4782 5180 5454 5601 6012 2526 Size: 758 1498 398 274 147 411 2863 Fragments arranged by size: 2863 1498 758 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6124 Fragments arranged by size: 6124 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2111 2275 2402 2858 2924 3115 1186 Size: 925 164 127 456 66 191 4420 Fragments arranged by size: 4420 925 456 191 164 127 66 BseYI C'CCAG_C Cuts at: 2365 2398 2424 3261 5552 6048 2365 Size: 33 26 837 2291 496 2666 Fragments arranged by size: 2666 2291 837 496 33 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3143 3530 1163 Size: 573 1407 387 3982 Fragments arranged by size: 3982 1407 573 387 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2924 3799 4560 5588 1540 Size: 275 186 923 875 761 1028 2301 Fragments arranged by size: 2301 1028 923 875 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2490 3486 3612 4410 4495 5566 2490 Size: 996 126 798 85 1071 3273 Fragments arranged by size: 3273 1071 996 798 126 85 BsmI GAATG_Cn' Cuts at: 2932 3860 3953 2932 Size: 928 93 5328 Fragments arranged by size: 5328 928 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2054 2054 Size: 6349 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2120 2133 2490 2689 2951 3292 Size: 399 234 96 13 357 199 262 341 Cuts at: 3292 3324 3486 3537 3612 3668 4410 4495 5566 Size: 32 162 51 75 56 742 85 1071 Cuts at: 5566 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 357 341 262 234 199 162 96 85 75 56 51 32 13 BspEI T'CCGG_A Cuts at: 2526 6012 2526 Size: 3486 2863 BspHI T'CATG_A Cuts at: 4238 5968 4238 Size: 1730 4619 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3704 844 Size: 2860 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3428 4236 1394 Size: 429 1605 808 3507 Fragments arranged by size: 3507 1605 808 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2799 3354 4844 5018 65 Size: 1183 1551 555 1490 174 1396 Fragments arranged by size: 1551 1490 1396 1183 555 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4993 5180 1700 Size: 110 3183 187 2869 Fragments arranged by size: 3183 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6349 BssHII G'CGCG_C Cuts at: 1925 3072 1925 Size: 1147 5202 BssSI C'ACGA_G Cuts at: 4409 5421 4409 Size: 1012 5337 BstAPI GCAn_nnn'nTGC Cuts at: 794 3031 794 Size: 2237 4112 BstBI TT'CG_AA Cuts at: 5153 5153 Size: 6349 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6349 BstXI CCAn_nnnn'nTGG Cuts at: 2470 3583 2470 Size: 1113 5236 BstYI r'GATC_y Cuts at: 1972 3679 4046 4431 4448 5900 5986 5998 6344 Size: 1707 367 385 17 1452 86 12 346 Cuts at: 6344 1972 Size: 1977 Fragments arranged by size: 1977 1707 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2618 2618 Size: 6349 BtgI C'CryG_G Cuts at: 513 1065 2042 2112 2251 2952 3151 3582 513 Size: 552 977 70 139 701 199 431 3280 Fragments arranged by size: 3280 977 701 552 431 199 139 70 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2477 487 Size: 683 56 1251 4359 Fragments arranged by size: 4359 1251 683 56 BtsI GCAGTG_nn' Cuts at: 2234 3020 3134 3629 3865 4659 4679 2234 Size: 786 114 495 236 794 20 3904 Fragments arranged by size: 3904 794 786 495 236 114 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6349 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6314 DraI TTT'AAA Cuts at: 3653 3653 Size: 6349 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5356 809 Size: 507 4040 1802 Fragments arranged by size: 4040 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3078 3195 3360 Size: 54 1425 50 275 18 1248 117 165 Cuts at: 3360 3519 3796 4685 6314 8 Size: 159 277 889 1629 43 Fragments arranged by size: 1629 1425 1248 889 277 275 165 159 117 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3796 1537 Size: 275 1984 4090 Fragments arranged by size: 4090 1984 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3305 3332 3461 3555 4279 1353 Size: 1952 27 129 94 724 3423 Fragments arranged by size: 3423 1952 724 129 94 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3335 4936 5310 5456 387 Size: 1064 138 1746 1601 374 146 1280 Fragments arranged by size: 1746 1601 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3610 3610 Size: 6349 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2261 2367 2560 2582 2741 2981 2990 Size: 393 629 106 193 22 159 240 9 Cuts at: 2990 3314 4412 5009 5796 6064 1239 Size: 324 1098 597 787 268 1524 Fragments arranged by size: 1524 1098 787 629 597 393 324 268 240 193 159 106 22 9 EcoO109I rG'GnC_Cy Cuts at: 2038 2245 2311 2395 2686 2948 2038 Size: 207 66 84 291 262 5439 Fragments arranged by size: 5439 291 262 207 84 66 EcoRI G'AATT_C Cuts at: 3658 4036 3658 Size: 378 5971 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2281 3319 2281 Size: 1038 5311 FspI TGC'GCA Cuts at: 4855 4855 Size: 6349 HaeII r_GCGC'y Cuts at: 1678 2887 3241 3262 5496 1678 Size: 1209 354 21 2234 2531 Fragments arranged by size: 2531 2234 1209 354 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2858 2890 4990 5022 5064 Size: 32 135 32 1286 32 2100 32 42 Cuts at: 5064 5096 1373 Size: 32 2626 Fragments arranged by size: 2626 2100 1286 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3693 3939 669 Size: 988 281 1755 246 3079 Fragments arranged by size: 3079 1755 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6349 HpaI GTT'AAC Cuts at: 3939 3939 Size: 6349 KpnI G_GTAC'C Cuts at: 3674 3674 Size: 6349 MfeI C'AATT_G Cuts at: 3948 3948 Size: 6349 MluI A'CGCG_T Cuts at: 6225 6225 Size: 6349 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5463 5647 1305 Size: 4158 184 2007 Fragments arranged by size: 4158 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3197 3521 10 Size: 54 1425 343 1365 324 2838 Fragments arranged by size: 2838 1425 1365 343 324 54 MslI CAynn'nnrTG Cuts at: 518 1166 2541 3506 3575 3581 4307 4666 4825 Size: 648 1375 965 69 6 726 359 159 Cuts at: 4825 518 Size: 2042 Fragments arranged by size: 2042 1375 965 726 648 359 159 69 6 MspA1I CmG'CkG Cuts at: 1606 2044 2450 3153 3396 3765 4442 5590 5835 Size: 438 406 703 243 369 677 1148 245 Cuts at: 5835 1606 Size: 2120 Fragments arranged by size: 2120 1148 703 677 438 406 369 245 243 NcoI C'CATG_G Cuts at: 513 1065 3582 513 Size: 552 2517 3280 Fragments arranged by size: 3280 2517 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6349 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6349 NotI GC'GGCC_GC Cuts at: 3796 3796 Size: 6349 NspI r_CATG'y Cuts at: 1132 1229 3505 3574 3707 5252 1132 Size: 97 2276 69 133 1545 2229 Fragments arranged by size: 2276 2229 1545 133 97 69 PasI CC'CwG_GG Cuts at: 1348 2033 2944 1348 Size: 685 911 4753 Fragments arranged by size: 4753 911 685 PciI A'CATG_T Cuts at: 5248 5248 Size: 6349 PflMI CCAn_nnn'nTGG Cuts at: 1108 3604 1108 Size: 2496 3853 PfoI T'CCnGG_A Cuts at: 2549 2814 2549 Size: 265 6084 PmeI GTTT'AAAC Cuts at: 3653 3653 Size: 6349 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4418 4450 5931 5963 4418 Size: 32 1481 32 4804 Fragments arranged by size: 4804 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2038 2245 2311 2395 2038 Size: 207 66 84 5992 Fragments arranged by size: 5992 207 84 66 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6349 PsiI TTA'TAA Cuts at: 3919 3919 Size: 6349 PspOMI G'GGCC_C Cuts at: 2685 2947 3533 2685 Size: 262 586 5501 Fragments arranged by size: 5501 586 262 PstI C_TGCA'G Cuts at: 830 2611 3027 3621 3701 830 Size: 1781 416 594 80 3478 Fragments arranged by size: 3478 1781 594 416 80 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6349 PvuII CAG'CTG Cuts at: 2450 2450 Size: 6349 SacI G_AGCT'C Cuts at: 3612 3612 Size: 6349 SacII CC_GC'GG Cuts at: 2045 3154 2045 Size: 1109 5240 SalI G'TCGA_C Cuts at: 1936 3691 1936 Size: 1755 4594 SanDI GG'GwC_CC Cuts at: 2038 2038 Size: 6349 SapI GCTCTTCn'nnn_ Cuts at: 3305 3461 3305 Size: 156 6193 SbfI CC_TGCA'GG Cuts at: 3701 3701 Size: 6349 ScaI AGT'ACT Cuts at: 4597 6309 4597 Size: 1712 4637 SexAI A'CCwGG_T Cuts at: 3057 3597 3057 Size: 540 5809 SfcI C'TryA_G Cuts at: 826 1045 2607 3023 3617 3697 4832 5513 5704 Size: 219 1562 416 594 80 1135 681 191 Cuts at: 5704 826 Size: 1471 Fragments arranged by size: 1562 1471 1135 681 594 416 219 191 80 SfiI GGCCn_nnn'nGGCC Cuts at: 2955 2955 Size: 6349 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6349 SmaI CCC'GGG Cuts at: 1872 3676 1872 Size: 1804 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2971 4453 5354 5616 820 Size: 197 926 1028 1482 901 262 1553 Fragments arranged by size: 1553 1482 1028 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4140 493 Size: 3647 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6349 SphI G_CATG'C Cuts at: 1132 3574 3707 1132 Size: 2442 133 3774 Fragments arranged by size: 3774 2442 133 SspI AAT'ATT Cuts at: 5 52 4273 5 Size: 47 4221 2081 Fragments arranged by size: 4221 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6349 StyI C'CwwG_G Cuts at: 513 1065 2469 3582 3788 513 Size: 552 1404 1113 206 3074 Fragments arranged by size: 3074 1404 1113 552 206 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2221 2938 4376 4393 4546 4731 5176 Size: 1255 641 717 1438 17 153 185 445 Cuts at: 5176 325 Size: 1498 Fragments arranged by size: 1498 1438 1255 717 641 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2649 4595 6307 Size: 274 80 33 51 1443 672 1946 1712 Cuts at: 6307 96 Size: 138 Fragments arranged by size: 1946 1712 1443 672 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6309 6341 6309 Size: 32 6317 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6349 XbaI T'CTAG_A Cuts at: 3685 3685 Size: 6349 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6349 XmaI C'CCGG_G Cuts at: 1870 3674 1870 Size: 1804 4545 XmnI GAAnn'nnTTC Cuts at: 3299 4478 3299 Size: 1179 5170 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3271 4157 276 Size: 53 83 186 936 1737 886 2468 Fragments arranged by size: 2468 1737 936 886 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SfoI SrfI SwaI XcmI