Restriction map for
pFN21AE3557
(Linear) (Six-base) MAPSORT of: KIEE3557.seq Check: 8740 from: 1 to: 1644
KIEE3557.orf
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AatII G_ACGT'C
Cuts at: 0 1268 1644
Size: 1268 376
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1309 1644
Size: 1309 335
AfeI AGC'GCT
Cuts at: 0 880 1644
Size: 880 764
AhdI GACnn_n'nnGTC
Cuts at: 0 867 1644
Size: 867 777
AleI CACnn'nnGTG
Cuts at: 0 536 1644
Size: 536 1108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 554 1519 1644
Size: 554 965 125
Fragments arranged by size:
965 554 125
AlwNI CAG_nnn'CTG
Cuts at: 0 448 782 816 1026 1391 1644
Size: 448 334 34 210 365 253
Fragments arranged by size:
448 365 334 253 210 34
ApaI G_GGCC'C
Cuts at: 0 684 946 1532 1644
Size: 684 262 586 112
Fragments arranged by size:
684 586 262 112
AvaI C'yCGr_G
Cuts at: 0 52 67 88 1312 1546 1644
Size: 52 15 21 1224 234 98
Fragments arranged by size:
1224 234 98 52 21 15
BanI G'GyrC_C
Cuts at: 0 1104 1644
Size: 1104 540
BanII G_rGCy'C
Cuts at: 0 19 115 128 684 946 1287 1319 1532
Size: 19 96 13 556 262 341 32 213
Cuts at: 1532 1607 1644
Size: 75 37
Fragments arranged by size:
556 341 262 213 96 75 37 32
19 13
BbvCI CC'TCA_GC
Cuts at: 0 658 1644
Size: 658 986
BglI GCCn_nnn'nGGC
Cuts at: 0 734 950 1141 1644
Size: 734 216 191 503
Fragments arranged by size:
734 503 216 191
Bme1580I G_kGCm'C
Cuts at: 0 684 946 1532 1644
Size: 684 262 586 112
Fragments arranged by size:
684 586 262 112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 297 447 1644
Size: 297 150 1197
Fragments arranged by size:
1197 297 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 362 555 577 736 976 985 1644
Size: 256 106 193 22 159 240 9 659
Fragments arranged by size:
659 256 240 193 159 106 22 9
Bpu10I CC'TnA_GC
Cuts at: 0 13 658 800 1644
Size: 13 645 142 844
Fragments arranged by size:
844 645 142 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 987 1644
Size: 987 657
BsaI GGTCTCn'nnnn_
Cuts at: 0 1552 1644
Size: 1552 92
BsaHI Gr'CG_yC
Cuts at: 0 566 1265 1644
Size: 566 699 379
Fragments arranged by size:
699 566 379
BsaWI w'CCGG_w
Cuts at: 0 521 1279 1644
Size: 521 758 365
Fragments arranged by size:
758 521 365
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 270 397 853 919 1110 1644
Size: 106 164 127 456 66 191 534
Fragments arranged by size:
534 456 191 164 127 106 66
BseYI C'CCAG_C
Cuts at: 0 360 393 419 1256 1644
Size: 360 33 26 837 388
Fragments arranged by size:
837 388 360 33 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1138 1525 1644
Size: 1138 387 119
Fragments arranged by size:
1138 387 119
BsiEI CG_ry'CG
Cuts at: 0 919 1644
Size: 919 725
BsiHKAI G_wGCw'C
Cuts at: 0 485 1481 1607 1644
Size: 485 996 126 37
Fragments arranged by size:
996 485 126 37
BsmI GAATG_Cn'
Cuts at: 0 927 1644
Size: 927 717
BsmBI CGTCTCn'nnnn_
Cuts at: 0 49 1644
Size: 49 1595
Bsp1286I G_dGCh'C
Cuts at: 0 19 115 128 485 684 946 1287 1319
Size: 19 96 13 357 199 262 341 32
Cuts at: 1319 1481 1532 1607 1644
Size: 162 51 75 37
Fragments arranged by size:
357 341 262 199 162 96 75 51
37 32 19 13
BspEI T'CCGG_A
Cuts at: 0 521 1644
Size: 521 1123
BsrBI CCG'CTC
Cuts at: 0 1423 1644
Size: 1423 221
BsrDI GCAATG_nn'
Cuts at: 0 794 1349 1644
Size: 794 555 295
Fragments arranged by size:
794 555 295
BssHII G'CGCG_C
Cuts at: 0 1067 1644
Size: 1067 577
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1026 1644
Size: 1026 618
BstXI CCAn_nnnn'nTGG
Cuts at: 0 465 1578 1644
Size: 465 1113 66
Fragments arranged by size:
1113 465 66
Bsu36I CC'TnA_GG
Cuts at: 0 613 1644
Size: 613 1031
BtgI C'CryG_G
Cuts at: 0 37 107 246 947 1146 1577 1644
Size: 37 70 139 701 199 431 67
Fragments arranged by size:
701 431 199 139 70 67 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 472 1644
Size: 472 1172
BtsI GCAGTG_nn'
Cuts at: 0 229 1015 1129 1624 1644
Size: 229 786 114 495 20
Fragments arranged by size:
786 495 229 114 20
EaeI y'GGCC_r
Cuts at: 0 1073 1190 1355 1514 1644
Size: 1073 117 165 159 130
Fragments arranged by size:
1073 165 159 130 117
EarI CTCTTCn'nnn_
Cuts at: 0 1300 1327 1456 1550 1644
Size: 1300 27 129 94 94
Fragments arranged by size:
1300 129 94 94 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1330 1644
Size: 1330 314
EcoICRI GAG'CTC
Cuts at: 0 1605 1644
Size: 1605 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 362 555 577 736 976 985 1309
Size: 256 106 193 22 159 240 9 324
Cuts at: 1309 1644
Size: 335
Fragments arranged by size:
335 324 256 240 193 159 106 22
9
EcoO109I rG'GnC_Cy
Cuts at: 0 33 240 306 390 681 943 1644
Size: 33 207 66 84 291 262 701
Fragments arranged by size:
701 291 262 207 84 66 33
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 276 1314 1644
Size: 276 1038 330
Fragments arranged by size:
1038 330 276
HaeII r_GCGC'y
Cuts at: 0 882 1236 1257 1644
Size: 882 354 21 387
Fragments arranged by size:
882 387 354 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 853 885 1644
Size: 853 32 759
Fragments arranged by size:
853 759 32
MscI TGG'CCA
Cuts at: 0 1192 1516 1644
Size: 1192 324 128
Fragments arranged by size:
1192 324 128
MslI CAynn'nnrTG
Cuts at: 0 536 1501 1570 1576 1644
Size: 536 965 69 6 68
Fragments arranged by size:
965 536 69 68 6
MspA1I CmG'CkG
Cuts at: 0 39 445 1148 1391 1644
Size: 39 406 703 243 253
Fragments arranged by size:
703 406 253 243 39
NcoI C'CATG_G
Cuts at: 0 1577 1644
Size: 1577 67
NspI r_CATG'y
Cuts at: 0 1500 1569 1644
Size: 1500 69 75
Fragments arranged by size:
1500 75 69
PasI CC'CwG_GG
Cuts at: 0 28 939 1644
Size: 28 911 705
Fragments arranged by size:
911 705 28
PflMI CCAn_nnn'nTGG
Cuts at: 0 1599 1644
Size: 1599 45
PfoI T'CCnGG_A
Cuts at: 0 544 809 1644
Size: 544 265 835
Fragments arranged by size:
835 544 265
PpuMI rG'GwC_Cy
Cuts at: 0 33 240 306 390 1644
Size: 33 207 66 84 1254
Fragments arranged by size:
1254 207 84 66 33
PspOMI G'GGCC_C
Cuts at: 0 680 942 1528 1644
Size: 680 262 586 116
Fragments arranged by size:
680 586 262 116
PstI C_TGCA'G
Cuts at: 0 606 1022 1616 1644
Size: 606 416 594 28
Fragments arranged by size:
606 594 416 28
PvuII CAG'CTG
Cuts at: 0 445 1644
Size: 445 1199
SacI G_AGCT'C
Cuts at: 0 1607 1644
Size: 1607 37
SacII CC_GC'GG
Cuts at: 0 40 1149 1644
Size: 40 1109 495
Fragments arranged by size:
1109 495 40
SanDI GG'GwC_CC
Cuts at: 0 33 1644
Size: 33 1611
SapI GCTCTTCn'nnn_
Cuts at: 0 1300 1456 1644
Size: 1300 156 188
Fragments arranged by size:
1300 188 156
SexAI A'CCwGG_T
Cuts at: 0 1052 1592 1644
Size: 1052 540 52
Fragments arranged by size:
1052 540 52
SfcI C'TryA_G
Cuts at: 0 602 1018 1612 1644
Size: 602 416 594 32
Fragments arranged by size:
602 594 416 32
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 950 1644
Size: 950 694
SmlI C'TyrA_G
Cuts at: 0 966 1644
Size: 966 678
SphI G_CATG'C
Cuts at: 0 1569 1644
Size: 1569 75
StyI C'CwwG_G
Cuts at: 0 464 1577 1644
Size: 464 1113 67
Fragments arranged by size:
1113 464 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 216 933 1644
Size: 216 717 711
Fragments arranged by size:
717 711 216
TatI w'GTAC_w
Cuts at: 0 644 1644
Size: 644 1000
XmnI GAAnn'nnTTC
Cuts at: 0 1294 1644
Size: 1294 350
ZraI GAC'GTC
Cuts at: 0 1266 1644
Size: 1266 378
Enzymes that do cut:
AatII AcuI AfeI AhdI AleI AlfI AlwNI ApaI
AvaI BanI BanII BbvCI BglI Bme1580I BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrBI BsrDI
BssHII BstAPI BstXI Bsu36I BtgI BtgZI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I
MscI MslI MspA1I NcoI NspI PasI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI
SexAI SfcI SfiI SmlI SphI StyI TaqII TatI
XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AgeI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BsaAI BsaBI BsaXI BsiWI BspHI
BspMI BsrFI BsrGI BssSI BstBI BstEII BstYI BstZ17I
ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SbfI ScaI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI