GNAL ORF clone pFN21AE3195

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE3195

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Clone info.

Species

human

Clone Name

pFN21AE3195 Sequence (with vector) Restriction Map

Product ID

FHC26254

ORF Sequence

DNA sequence (1374 bp)
Protein sequence (458 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3195

Symbol

GNAL

Alias : DYT25

Description

guanine nucleotide binding protein (G protein), alpha activating activity polypeptide, olfactory type, transcript variant 1

Original Clone ID

cp93195

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1374 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 458 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11851.1 8.4e-135 100.0 GNAL CCDS11852.1 4.4e-100 95.0 GNAL CCDS42892.1 1.9e-84 76.4 GNAS CCDS46624.1 6.7e-84 76.2 GNAS CCDS46623.1 1.9e-75 73.4 GNAS The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001019 121 136 PR00318 Guanine nucleotide binding protein (G-protein) IPR000367 150 164 PR00443 G-protein alpha subunit IPR000367 165 180 PR00443 G-protein alpha subunit IPR000367 181 195 PR00443 G-protein alpha subunit IPR001019 254 276 PR00318 Guanine nucleotide binding protein (G-protein) IPR001019 283 300 PR00318 Guanine nucleotide binding protein (G-protein) IPR001019 305 333 PR00318 Guanine nucleotide binding protein (G-protein) IPR000367 334 349 PR00443 G-protein alpha subunit IPR001019 351 360 PR00318 Guanine nucleotide binding protein (G-protein) IPR000367 398 412 PR00443 G-protein alpha subunit IPR000367 413 426 PR00443 G-protein alpha subunit HMMPfam IPR001019 94 457 PF00503 Guanine nucleotide binding protein (G-protein) HMMSmart IPR001019 99 457 SM00275 Guanine nucleotide binding protein (G-protein)
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_892023 458 139312,615073 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3195 Download

>KIEE3195  1374 bp
ATGGGTCTGTGCTACAGTCTGCGGCCGCTGCTTTTCGGGGGCCCAGGGGACGACCCCTGC
GCGGCCTCGGAGCCGCCGGTGGAGGACGCGCAGCCCGCCCCGGCCCCGGCCCTGGCCCCA
GTCCGGGCGGCCGCAAGGGACACGGCCCGGACCCTGCTCCCTCGGGGCGGCGAAGGGAGC
CCGGCATGCGCTCGGCCCAAAGCAGACAAGCCGAAGGAGAAGCGGCAGCGCACCGAGCAG
CTTAGTGCCGAGGAGCGCGAGGCGGCCAAGGAGCGCGAGGCGGTCAAGGAGGCGAGGAAA
GTGAGCCGGGGCATCGACCGCATGCTGCGCGACCAGAAGCGCGACCTGCAGCAGACGCAC
CGGCTCCTGCTGCTCGGGGCTGGTGAGTCTGGGAAAAGCACTATCGTCAAACAGATGAGG
ATCCTGCACGTCAATGGGTTTAATCCCGAGGAAAAGAAACAGAAAATTCTGGACATCCGG
AAAAATGTTAAAGATGCTATCGTGACAATTGTTTCAGCAATGAGTACTATAATACCTCCA
GTTCCGCTGGCCAACCCTGAAAACCAATTTCGATCAGACTACATCAAGAGCATAGCCCCT
ATCACTGACTTTGAATATTCCCAGGAATTCTTTGACCATGTGAAAAAACTTTGGGACGAT
GAAGGCGTGAAGGCATGCTTTGAGAGATCCAACGAATACCAGCTGATTGACTGTGCACAA
TACTTCCTGGAAAGAATCGACAGCGTCAGCTTGGTTGACTACACACCCACAGACCAGGAC
CTCCTCAGATGCAGAGTTCTGACATCTGGGATTTTTGAGACACGATTCCAAGTGGACAAA
GTAAACTTCCACATGTTTGATGTTGGTGGCCAGAGGGATGAGAGGAGAAAATGGATCCAG
TGCTTTAACGATGTCACAGCTATCATTTACGTCGCAGCCTGCAGTAGCTACAACATGGTG
ATTCGAGAAGATAACAACACCAACAGGCTGAGAGAGTCCCTGGATCTTTTTGAAAGCATC
TGGAACAACAGGTGGTTACGGACCATTTCTATCATCTTGTTCTTGAACAAACAAGATATG
CTGGCAGAAAAAGTCTTGGCAGGGAAATCAAAAATTGAAGACTATTTCCCAGAATATGCA
AATTATACTGTTCCTGAAGACGCAACACCAGATGCAGGAGAAGATCCCAAAGTTACAAGA
GCCAAGTTCTTTATCCGGGACCTGTTTTTGAGGATCAGCACGGCCACCGGTGACGGCAAA
CATTACTGCTACCCGCACTTCACCTGCGCCGTGGACACAGAGAACATCCGCAGGGTGTTC
AACGACTGCCGCGACATCATCCAGCGGATGCACCTCAAGCAGTATGAGCTCTTG

ORF Protein

Cloned ORF protein sequence for pFN21AE3195 Download

>KIEE3195  458 aa
MGLCYSLRPLLFGGPGDDPCAASEPPVEDAQPAPAPALAPVRAAARDTARTLLPRGGEGS
PACARPKADKPKEKRQRTEQLSAEEREAAKEREAVKEARKVSRGIDRMLRDQKRDLQQTH
RLLLLGAGESGKSTIVKQMRILHVNGFNPEEKKQKILDIRKNVKDAIVTIVSAMSTIIPP
VPLANPENQFRSDYIKSIAPITDFEYSQEFFDHVKKLWDDEGVKACFERSNEYQLIDCAQ
YFLERIDSVSLVDYTPTDQDLLRCRVLTSGIFETRFQVDKVNFHMFDVGGQRDERRKWIQ
CFNDVTAIIYVAACSSYNMVIREDNNTNRLRESLDLFESIWNNRWLRTISIILFLNKQDM
LAEKVLAGKSKIEDYFPEYANYTVPEDATPDAGEDPKVTRAKFFIRDLFLRISTATGDGK
HYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLKQYELL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3195 Download

>pFN21AE3195  6079 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGTCTGTGCTACAGTCTGCGGCCGCTGCTTTT
CGGGGGCCCAGGGGACGACCCCTGCGCGGCCTCGGAGCCGCCGGTGGAGGACGCGCAGCC
CGCCCCGGCCCCGGCCCTGGCCCCAGTCCGGGCGGCCGCAAGGGACACGGCCCGGACCCT
GCTCCCTCGGGGCGGCGAAGGGAGCCCGGCATGCGCTCGGCCCAAAGCAGACAAGCCGAA
GGAGAAGCGGCAGCGCACCGAGCAGCTTAGTGCCGAGGAGCGCGAGGCGGCCAAGGAGCG
CGAGGCGGTCAAGGAGGCGAGGAAAGTGAGCCGGGGCATCGACCGCATGCTGCGCGACCA
GAAGCGCGACCTGCAGCAGACGCACCGGCTCCTGCTGCTCGGGGCTGGTGAGTCTGGGAA
AAGCACTATCGTCAAACAGATGAGGATCCTGCACGTCAATGGGTTTAATCCCGAGGAAAA
GAAACAGAAAATTCTGGACATCCGGAAAAATGTTAAAGATGCTATCGTGACAATTGTTTC
AGCAATGAGTACTATAATACCTCCAGTTCCGCTGGCCAACCCTGAAAACCAATTTCGATC
AGACTACATCAAGAGCATAGCCCCTATCACTGACTTTGAATATTCCCAGGAATTCTTTGA
CCATGTGAAAAAACTTTGGGACGATGAAGGCGTGAAGGCATGCTTTGAGAGATCCAACGA
ATACCAGCTGATTGACTGTGCACAATACTTCCTGGAAAGAATCGACAGCGTCAGCTTGGT
TGACTACACACCCACAGACCAGGACCTCCTCAGATGCAGAGTTCTGACATCTGGGATTTT
TGAGACACGATTCCAAGTGGACAAAGTAAACTTCCACATGTTTGATGTTGGTGGCCAGAG
GGATGAGAGGAGAAAATGGATCCAGTGCTTTAACGATGTCACAGCTATCATTTACGTCGC
AGCCTGCAGTAGCTACAACATGGTGATTCGAGAAGATAACAACACCAACAGGCTGAGAGA
GTCCCTGGATCTTTTTGAAAGCATCTGGAACAACAGGTGGTTACGGACCATTTCTATCAT
CTTGTTCTTGAACAAACAAGATATGCTGGCAGAAAAAGTCTTGGCAGGGAAATCAAAAAT
TGAAGACTATTTCCCAGAATATGCAAATTATACTGTTCCTGAAGACGCAACACCAGATGC
AGGAGAAGATCCCAAAGTTACAAGAGCCAAGTTCTTTATCCGGGACCTGTTTTTGAGGAT
CAGCACGGCCACCGGTGACGGCAAACATTACTGCTACCCGCACTTCACCTGCGCCGTGGA
CACAGAGAACATCCGCAGGGTGTTCAACGACTGCCGCGACATCATCCAGCGGATGCACCT
CAAGCAGTATGAGCTCTTGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT