################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AhdI AlwNI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MaeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccIII 476 TCCGGA T^CCGGA AfaI 524 GTAC GT^AC AflIII 851 ACATGT A^C[GA][CT]GT AgeI 1246 ACCGGT A^CCGGT Alw44I 713 GTGCAC G^TGCAC ApaI 39 GGGCCC GGGCC^C ApaLI 713 GTGCAC G^TGCAC AspI 739 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BpmI 536 CTCCAG CTGGAG(16/14) BsaBI 414 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 476 TCCGGA T^CCGGA BsgI 424 CTGCAC GTGCAG(16/14) BsmAI 817 GAGAC GTCTC(1/5) BspEI 476 TCCGGA T^CCGGA BspLU11I 851 ACATGT A^CATGT BsrDI 517 GCAATG GCAATG(2/0) DsaI 1289 CCGTGG C^C[GA][CT]GG EcoRI 625 GAATTC G^AATTC EcoT14I 266 CCAAGG C^C[AT][AT]GG HincII 754 GTTGAC GT[CT]^[GA]AC HindII 754 GTTGAC GT[CT]^[GA]AC MamI 414 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 506 CAATTG C^AATTG MroI 476 TCCGGA T^CCGGA MslI 427 CACGTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 506 CAATTG C^AATTG PflFI 739 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1246 ACCGGT A^CCGGT PvuII 700 CAGCTG CAG^CTG RsaI 524 GTAC GT^AC SacI 1366 GAGCTC GAGCT^C ScaI 523 AGTACT AGT^ACT SmlI 1354 CTCAAG C^T[CT][GA]AG SspI 614 AATATT AAT^ATT StyI 266 CCAAGG C^C[AT][AT]GG Tth111I 739 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 464 AAATTC [GA]^AATT[CT] 625 GAATTC ApoI 464 AAATTC [GA]^AATT[CT] 625 GAATTC AspHI 713 GTGCAC G[AT]GC[AT]^C 1366 GAGCTC BalI 548 TGGCCA TGG^CCA 867 TGGCCA BamHI 419 GGATCC G^GATCC 893 GGATCC BbsI 1117 GAAGAC GAAGAC(2/6) 1156 GAAGAC BpuAI 1117 GAAGAC GAAGAC(2/6) 1156 GAAGAC BsaWI 476 TCCGGA [AT]^CCGG[AT] 1246 ACCGGT BsiHKAI 713 GTGCAC G[AT]GC[AT]^C 1366 GAGCTC BspMI 344 ACCTGC ACCTGC(4/8) 1282 ACCTGC EagI 22 CGGCCG C^GGCCG 128 CGGCCG EclXI 22 CGGCCG C^GGCCG 128 CGGCCG Eco52I 22 CGGCCG C^GGCCG 128 CGGCCG FauI 94 CCCGC CCCGC(4/6) 1272 CCCGC MaeII 428 ACGT A^CGT 929 ACGT MluNI 548 TGGCCA TGG^CCA 867 TGGCCA MscI 548 TGGCCA TGG^CCA 867 TGGCCA NotI 21 GCGGCCGC GC^GGCCGC 127 GCGGCCGC PleI 385 GAGTC GAGTC(4/5) 994 GAGTC PpuMI 776 AGGACCT [GA]G^G[AT]CC[CT] 1217 GGGACCT PstI 346 CTGCAG CTGCA^G 939 CTGCAG SgrAI 74 CGCCGGTG C[GA]^CCGG[CT]G 1245 CACCGGTG TspRI 603 CACTG CA[GC]TG[ACGT][ACGT]^ 898 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AccII AciI AluI AlwI AvaI AvaII BanII BbvI BcnI BmyI BsaJI BseRI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI Sau3AI Sau96I ScrFI SfaNI SfcI SphI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TthHB8I XhoII