(Circular) (Six-base) MAPSORT of: pFN21AE3195.seq Check: 5486 from: 1 to: 6079 pFN21AE3195 6079 bp With 182 enzymes: SgfI * March 28, 2012 12:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3296 3296 Size: 6079 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3889 278 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3422 1937 Size: 1485 4594 Acc65I G'GTAC_C Cuts at: 3400 3400 Size: 6079 AclI AA'CG_TT Cuts at: 1563 4206 4579 1563 Size: 2643 373 3063 Fragments arranged by size: 3063 2643 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3180 4142 5526 5794 1632 Size: 1548 962 1384 268 1917 Fragments arranged by size: 1917 1548 1384 962 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5882 AflIII A'CryG_T Cuts at: 1203 2856 4978 5955 1203 Size: 1653 2122 977 1327 Fragments arranged by size: 2122 1653 1327 977 AgeI A'CCGG_T Cuts at: 3251 4910 3251 Size: 1659 4420 AhdI GACnn_n'nnGTC Cuts at: 4808 4808 Size: 6079 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6079 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2265 1184 Size: 1081 4998 AlwNI CAG_nnn'CTG Cuts at: 3822 5394 3822 Size: 1572 4507 ApaI G_GGCC'C Cuts at: 2048 2048 Size: 6079 ApaLI G'TGCA_C Cuts at: 2718 4136 5292 2718 Size: 1418 1156 3505 Fragments arranged by size: 3505 1418 1156 ApoI r'AATT_y Cuts at: 1493 2469 2630 3388 3614 3766 5875 1493 Size: 976 161 758 226 152 2109 1697 Fragments arranged by size: 2109 1697 976 758 226 161 152 AseI AT'TA_AT Cuts at: 160 1032 4633 160 Size: 872 3601 1606 Fragments arranged by size: 3601 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 AvaI C'yCGr_G Cuts at: 1870 1943 2166 2378 2450 3404 1870 Size: 73 223 212 72 954 4545 Fragments arranged by size: 4545 954 223 212 73 72 BamHI G'GATC_C Cuts at: 2424 2898 3409 3776 2424 Size: 474 511 367 4727 Fragments arranged by size: 4727 511 474 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3400 4855 618 Size: 325 217 627 1613 1455 1842 Fragments arranged by size: 1842 1613 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2048 2186 3375 3398 1391 Size: 657 138 1189 23 4072 Fragments arranged by size: 4072 1189 657 138 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3129 3168 928 Size: 971 1230 39 3839 Fragments arranged by size: 3839 1230 971 39 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4270 4304 1903 Size: 34 2333 34 3678 Fragments arranged by size: 3678 2333 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3971 5181 3971 Size: 1210 4869 BclI T'GATC_A Cuts at: 1548 1548 Size: 6079 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4690 136 Size: 107 122 71 631 605 3018 1525 Fragments arranged by size: 3018 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6074 6074 Size: 6079 BlpI GC'TnA_GC Cuts at: 3496 3496 Size: 6079 Bme1580I G_kGCm'C Cuts at: 1790 2048 2722 4140 5296 1790 Size: 258 674 1418 1156 2573 Fragments arranged by size: 2573 1418 1156 674 258 BmgBI CAC'GTC Cuts at: 1318 2434 1318 Size: 1116 4963 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2117 3810 4768 Size: 416 53 83 399 24 700 1693 958 Cuts at: 4768 442 Size: 1753 Fragments arranged by size: 1753 1693 958 700 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6079 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2526 4739 1239 Size: 1287 2213 2579 Fragments arranged by size: 2579 2213 1287 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3344 4204 5069 5367 3344 Size: 860 865 298 4056 Fragments arranged by size: 4056 865 860 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4742 882 Size: 617 3243 2219 Fragments arranged by size: 3243 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3870 493 Size: 711 2666 2702 Fragments arranged by size: 2702 2666 711 BsaBI GATnn'nnATC Cuts at: 2423 6073 2423 Size: 3650 2429 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3886 4268 4903 275 Size: 53 83 186 936 2353 382 635 1451 Fragments arranged by size: 2353 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2481 3251 4512 4910 5184 5331 5742 2481 Size: 770 1261 398 274 147 411 2818 Fragments arranged by size: 2818 1261 770 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5854 Fragments arranged by size: 5854 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2270 2777 2902 1186 Size: 1084 507 125 4363 Fragments arranged by size: 4363 1084 507 125 BseYI C'CCAG_C Cuts at: 5282 5778 5282 Size: 496 5583 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2414 1163 Size: 573 678 4828 Fragments arranged by size: 4828 678 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2030 2136 2323 3529 4290 5318 Size: 275 186 29 106 187 1206 761 1028 Cuts at: 5318 1540 Size: 2301 Fragments arranged by size: 2301 1206 1028 761 275 187 186 106 29 BsiHKAI G_wGCw'C Cuts at: 2722 3375 4140 4225 5296 2722 Size: 653 765 85 1071 3505 Fragments arranged by size: 3505 1071 765 653 85 BsmI GAATG_Cn' Cuts at: 3590 3683 3590 Size: 93 5986 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2186 2722 3375 3398 4140 4225 Size: 399 258 138 536 653 23 742 85 Cuts at: 4225 5296 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 653 536 399 258 138 85 23 BspEI T'CCGG_A Cuts at: 2481 5742 2481 Size: 3261 2818 BspHI T'CATG_A Cuts at: 3968 5698 3968 Size: 1730 4349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2358 3296 3434 844 Size: 1514 938 138 3489 Fragments arranged by size: 3489 1514 938 138 BsrBI CCG'CTC Cuts at: 1394 1823 3966 1394 Size: 429 2143 3507 Fragments arranged by size: 3507 2143 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2529 4574 4748 65 Size: 1183 1281 2045 174 1396 Fragments arranged by size: 2045 1396 1281 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2080 2364 3251 4723 4910 1700 Size: 110 270 284 887 1472 187 2869 Fragments arranged by size: 2869 1472 887 284 270 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6079 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6079 BssSI C'ACGA_G Cuts at: 4139 5151 4139 Size: 1012 5067 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6079 BstBI TT'CG_AA Cuts at: 4883 4883 Size: 6079 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6079 BstYI r'GATC_y Cuts at: 1972 2424 2690 2898 3007 3187 3409 3776 4161 Size: 452 266 208 109 180 222 367 385 Cuts at: 4161 4178 5630 5716 5728 6074 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 452 385 367 346 266 222 208 180 109 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3294 513 Size: 552 939 1290 3298 Fragments arranged by size: 3298 1290 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5340 Fragments arranged by size: 5340 683 56 BtsI GCAGTG_nn' Cuts at: 3595 4389 4409 3595 Size: 794 20 5265 Fragments arranged by size: 5265 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6079 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6044 DraI TTT'AAA Cuts at: 3383 3383 Size: 6079 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5086 809 Size: 507 3770 1802 Fragments arranged by size: 3770 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2027 2133 2268 Size: 54 1425 50 275 18 197 106 135 Cuts at: 2268 2553 2872 3246 3526 4415 6044 8 Size: 285 319 374 280 889 1629 43 Fragments arranged by size: 1629 1425 889 374 319 285 280 275 197 135 106 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2027 2133 3526 1537 Size: 275 215 106 1393 4090 Fragments arranged by size: 4090 1393 275 215 106 EarI CTCTTCn'nnn_ Cuts at: 1353 4009 1353 Size: 2656 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4666 5040 5186 387 Size: 1064 138 3077 374 146 1280 Fragments arranged by size: 3077 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3373 3373 Size: 6079 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2526 3180 4142 4739 5526 5794 1239 Size: 393 894 654 962 597 787 268 1524 Fragments arranged by size: 1524 962 894 787 654 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2044 2782 3223 2044 Size: 738 441 4900 Fragments arranged by size: 4900 738 441 EcoRI G'AATT_C Cuts at: 2630 3388 3766 2630 Size: 758 378 4943 Fragments arranged by size: 4943 758 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2698 2698 Size: 6079 FspI TGC'GCA Cuts at: 4585 4585 Size: 6079 HaeII r_GCGC'y Cuts at: 1678 5226 1678 Size: 3548 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4720 4752 4794 4826 1373 Size: 32 135 32 3148 32 42 32 2626 Fragments arranged by size: 3148 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2761 3423 3669 669 Size: 988 281 823 662 246 3079 Fragments arranged by size: 3079 988 823 662 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6079 HpaI GTT'AAC Cuts at: 3669 3669 Size: 6079 KpnI G_GTAC'C Cuts at: 3404 3404 Size: 6079 MfeI C'AATT_G Cuts at: 2511 3678 2511 Size: 1167 4912 MluI A'CGCG_T Cuts at: 5955 5955 Size: 6079 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2718 5193 5377 1305 Size: 1413 2475 184 2007 Fragments arranged by size: 2475 2007 1413 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2555 2874 10 Size: 54 1425 343 723 319 3215 Fragments arranged by size: 3215 1425 723 343 319 54 MslI CAynn'nnrTG Cuts at: 518 1166 2436 4037 4396 4555 518 Size: 648 1270 1601 359 159 2042 Fragments arranged by size: 2042 1601 1270 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2032 2551 2707 3349 3495 4172 5320 5565 Size: 426 519 156 642 146 677 1148 245 Cuts at: 5565 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 642 519 426 245 156 146 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4588 Fragments arranged by size: 4588 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6079 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6079 NotI GC'GGCC_GC Cuts at: 2027 2133 3526 2027 Size: 106 1393 4580 Fragments arranged by size: 4580 1393 106 NspI r_CATG'y Cuts at: 1132 1229 2193 2329 2682 2860 3437 4982 1132 Size: 97 964 136 353 178 577 1545 2229 Fragments arranged by size: 2229 1545 964 577 353 178 136 97 PasI CC'CwG_GG Cuts at: 1348 2048 1348 Size: 700 5379 PciI A'CATG_T Cuts at: 2856 4978 2856 Size: 2122 3957 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6079 PfoI T'CCnGG_A Cuts at: 2730 3219 2730 Size: 489 5590 PmeI GTTT'AAAC Cuts at: 3383 3383 Size: 6079 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4148 4180 5661 5693 4148 Size: 32 1481 32 4534 Fragments arranged by size: 4534 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2782 3223 2782 Size: 441 5638 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6079 PsiI TTA'TAA Cuts at: 3649 3649 Size: 6079 PspOMI G'GGCC_C Cuts at: 2044 2044 Size: 6079 PstI C_TGCA'G Cuts at: 830 2355 2948 3431 830 Size: 1525 593 483 3478 Fragments arranged by size: 3478 1525 593 483 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6079 PvuII CAG'CTG Cuts at: 2707 2707 Size: 6079 SacI G_AGCT'C Cuts at: 3375 3375 Size: 6079 SalI G'TCGA_C Cuts at: 1936 3421 1936 Size: 1485 4594 SbfI CC_TGCA'GG Cuts at: 3431 3431 Size: 6079 ScaI AGT'ACT Cuts at: 2530 4327 6039 2530 Size: 1797 1712 2570 Fragments arranged by size: 2570 1797 1712 SfcI C'TryA_G Cuts at: 826 1045 2017 2351 2944 3427 4562 5243 5434 Size: 219 972 334 593 483 1135 681 191 Cuts at: 5434 826 Size: 1471 Fragments arranged by size: 1471 1135 972 681 593 483 334 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 SgrAI Cr'CCGG_yG Cuts at: 1700 2080 3251 1700 Size: 380 1171 4528 Fragments arranged by size: 4528 1171 380 SmaI CCC'GGG Cuts at: 1872 3406 1872 Size: 1534 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3359 4183 5084 5346 820 Size: 197 926 1416 824 901 262 1553 Fragments arranged by size: 1553 1416 926 901 824 262 197 SnaBI TAC'GTA Cuts at: 493 3870 493 Size: 3377 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6079 SphI G_CATG'C Cuts at: 1132 2193 2329 2682 3437 1132 Size: 1061 136 353 755 3774 Fragments arranged by size: 3774 1061 755 353 136 SspI AAT'ATT Cuts at: 5 52 2621 4003 5 Size: 47 2569 1382 2081 Fragments arranged by size: 2569 2081 1382 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6079 StyI C'CwwG_G Cuts at: 513 1065 2004 2271 3518 513 Size: 552 939 267 1247 3074 Fragments arranged by size: 3074 1247 939 552 267 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2785 4106 4123 4276 4461 4906 325 Size: 1255 1205 1321 17 153 185 445 1498 Fragments arranged by size: 1498 1321 1255 1205 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2528 4325 6037 Size: 274 80 33 51 1443 551 1797 1712 Cuts at: 6037 96 Size: 138 Fragments arranged by size: 1797 1712 1443 551 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2712 2744 6039 6071 2712 Size: 32 3295 32 2720 Fragments arranged by size: 3295 2720 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2747 1316 Size: 1431 4648 XbaI T'CTAG_A Cuts at: 3415 3415 Size: 6079 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6079 XmaI C'CCGG_G Cuts at: 1870 3404 1870 Size: 1534 4545 XmnI GAAnn'nnTTC Cuts at: 4208 4208 Size: 6079 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3887 276 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI