HACD3 ORF clone pFN21AE3148

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Halotag ORF Clone pFN21AE3148

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Clone info.

Species

human

Clone Name

pFN21AE3148 Sequence (with vector) Restriction Map

Product ID

FHC30622

Flexi Clone PID

FXC30622

ORF Sequence

DNA sequence (1086 bp)
Protein sequence (362 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3148

Symbol

HACD3

Alias : B-IND1, BIND1, HSPC121, PTPLAD1

Description

3-hydroxyacyl-CoA dehydratase 3

Original Clone ID

cp93148

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1086 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 362 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS45282.1 1.7e-152 100.0 HACD3 CCDS43791.1 2.6e-37 42.5 HACD4 CCDS46895.1 1.1e-18 28.6 HACD2 CCDS7121.1 2.8e-17 27.8 HACD1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR017447 9 84 PF04969 CS domain IPR007482 195 357 PF04387 Protein-tyrosine phosphatase-like ProfileScan IPR007052 5 94 PS51203 CS-like domain
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 148 GYLFMYNLVQFLGFSWIFVNLTV 170 SECONDARY 23 2 193 FCQMLAVVETINAAIGVTTSPVL 215 SECONDARY 23 3 230 IIFGTMEEMQNKAVVFFVFYLWS 252 PRIMARY 23 4 282 WIPLYPLGCLAEAVSVIQSIPIF 304 PRIMARY 23 5 323 RFSFFLQIYLIMIFLGLYINFR 344 PRIMARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_057479 362 615940 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3148 Download

>KIEE3148  1086 bp
ATGGAGAATCAGGTGTTGACGCCGCATGTCTACTGGGCTCAGCGACACCGCGAGCTATAT
CTGCGCGTGGAGCTGAGTGACGTACAGAACCCTGCCATCAGCATCACTGAAAACGTGCTG
CATTTCAAAGCTCAAGGACATGGTGCCAAAGGAGACAATGTCTATGAATTTCACCTGGAG
TTCTTAGACCTTGTGAAACCAGAGCCTGTTTACAAACTGACCCAGAGGCAGGTAAACATT
ACAGTACAGAAGAAAGTGAGTCAGTGGTGGGAGAGACTCACAAAGCAGGAAAAGCGACCA
CTGTTTTTGGCTCCTGACTTTGATCGTTGGCTGGATGAATCTGATGCGGAAATGGAGCTC
AGAGCTAAGGAAGAAGAGCGCCTAAATAAACTCCGACTGGAAAGCGAAGGCTCTCCTGAA
ACTCTTACAAACTTAAGGAAAGGATACCTGTTTATGTATAATCTTGTGCAATTCTTGGGA
TTCTCCTGGATCTTTGTCAACCTGACTGTGCGATTCTGTATCTTGGGAAAAGAGTCCTTT
TATGACACATTCCATACTGTGGCTGACATGATGTATTTCTGCCAGATGCTGGCAGTTGTG
GAAACTATCAATGCAGCAATTGGAGTCACTACGTCACCGGTGCTGCCTTCTCTGATCCAG
CTTCTTGGAAGAAATTTTATTTTGTTTATCATCTTTGGCACCATGGAAGAAATGCAGAAC
AAAGCTGTGGTTTTCTTTGTGTTTTATTTGTGGAGTGCAATTGAAATTTTCAGGTACTCT
TTCTACATGCTGACGTGCATTGACATGGATTGGAAGGTGCTCACATGGCTTCGTTACACT
CTGTGGATTCCCTTATATCCACTGGGATGTTTGGCGGAAGCTGTCTCAGTGATTCAGTCC
ATTCCAATATTCAATGAGACCGGACGATTCAGTTTCACATTGCCATATCCAGTGAAAATC
AAAGTTAGATTTTCCTTTTTTCTTCAGATTTATCTTATAATGATATTTTTAGGTTTATAC
ATAAATTTTCGTCACCTTTATAAACAGCGCAGACGGCGCTATGGACAAAAAAAGAAAAAG
ATCCAC

ORF Protein

Cloned ORF protein sequence for pFN21AE3148 Download

>KIEE3148  362 aa
MENQVLTPHVYWAQRHRELYLRVELSDVQNPAISITENVLHFKAQGHGAKGDNVYEFHLE
FLDLVKPEPVYKLTQRQVNITVQKKVSQWWERLTKQEKRPLFLAPDFDRWLDESDAEMEL
RAKEEERLNKLRLESEGSPETLTNLRKGYLFMYNLVQFLGFSWIFVNLTVRFCILGKESF
YDTFHTVADMMYFCQMLAVVETINAAIGVTTSPVLPSLIQLLGRNFILFIIFGTMEEMQN
KAVVFFVFYLWSAIEIFRYSFYMLTCIDMDWKVLTWLRYTLWIPLYPLGCLAEAVSVIQS
IPIFNETGRFSFTLPYPVKIKVRFSFFLQIYLIMIFLGLYINFRHLYKQRRRRYGQKKKK
IH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3148 Download

>pFN21AE3148  5791 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGAATCAGGTGTTGACGCCGCATGTCTACTG
GGCTCAGCGACACCGCGAGCTATATCTGCGCGTGGAGCTGAGTGACGTACAGAACCCTGC
CATCAGCATCACTGAAAACGTGCTGCATTTCAAAGCTCAAGGACATGGTGCCAAAGGAGA
CAATGTCTATGAATTTCACCTGGAGTTCTTAGACCTTGTGAAACCAGAGCCTGTTTACAA
ACTGACCCAGAGGCAGGTAAACATTACAGTACAGAAGAAAGTGAGTCAGTGGTGGGAGAG
ACTCACAAAGCAGGAAAAGCGACCACTGTTTTTGGCTCCTGACTTTGATCGTTGGCTGGA
TGAATCTGATGCGGAAATGGAGCTCAGAGCTAAGGAAGAAGAGCGCCTAAATAAACTCCG
ACTGGAAAGCGAAGGCTCTCCTGAAACTCTTACAAACTTAAGGAAAGGATACCTGTTTAT
GTATAATCTTGTGCAATTCTTGGGATTCTCCTGGATCTTTGTCAACCTGACTGTGCGATT
CTGTATCTTGGGAAAAGAGTCCTTTTATGACACATTCCATACTGTGGCTGACATGATGTA
TTTCTGCCAGATGCTGGCAGTTGTGGAAACTATCAATGCAGCAATTGGAGTCACTACGTC
ACCGGTGCTGCCTTCTCTGATCCAGCTTCTTGGAAGAAATTTTATTTTGTTTATCATCTT
TGGCACCATGGAAGAAATGCAGAACAAAGCTGTGGTTTTCTTTGTGTTTTATTTGTGGAG
TGCAATTGAAATTTTCAGGTACTCTTTCTACATGCTGACGTGCATTGACATGGATTGGAA
GGTGCTCACATGGCTTCGTTACACTCTGTGGATTCCCTTATATCCACTGGGATGTTTGGC
GGAAGCTGTCTCAGTGATTCAGTCCATTCCAATATTCAATGAGACCGGACGATTCAGTTT
CACATTGCCATATCCAGTGAAAATCAAAGTTAGATTTTCCTTTTTTCTTCAGATTTATCT
TATAATGATATTTTTAGGTTTATACATAAATTTTCGTCACCTTTATAAACAGCGCAGACG
GCGCTATGGACAAAAAAAGAAAAAGATCCACGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT