(Circular) (Six-base) MAPSORT of: pFN21AE3148.seq Check: 9788 from: 1 to: 5791 pFN21AE3148 5791 bp With 182 enzymes: SgfI * November 18, 2016 17:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3601 278 Size: 53 83 186 936 2065 2468 Fragments arranged by size: 2468 2065 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2034 3134 1937 Size: 97 1100 4594 Fragments arranged by size: 4594 1100 97 Acc65I G'GTAC_C Cuts at: 3112 3112 Size: 5791 AclI AA'CG_TT Cuts at: 1563 3918 4291 1563 Size: 2355 373 3063 Fragments arranged by size: 3063 2355 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2972 3854 5238 5506 1632 Size: 1340 882 1384 268 1917 Fragments arranged by size: 1917 1384 1340 882 268 AflII C'TTAA_G Cuts at: 820 1017 2437 820 Size: 197 1420 4174 Fragments arranged by size: 4174 1420 197 AflIII A'CryG_T Cuts at: 1203 4690 5667 1203 Size: 3487 977 1327 Fragments arranged by size: 3487 1327 977 AgeI A'CCGG_T Cuts at: 2641 4622 2641 Size: 1981 3810 AhdI GACnn_n'nnGTC Cuts at: 4520 4520 Size: 5791 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5791 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5791 AlwNI CAG_nnn'CTG Cuts at: 2210 2593 3534 5106 2210 Size: 383 941 1572 2895 Fragments arranged by size: 2895 1572 941 383 ApaLI G'TGCA_C Cuts at: 3848 5004 3848 Size: 1156 4635 ApoI r'AATT_y Cuts at: 1493 2171 2677 2769 3028 3100 3326 3478 5587 Size: 678 506 92 259 72 226 152 2109 Cuts at: 5587 1493 Size: 1697 Fragments arranged by size: 2109 1697 678 506 259 226 152 92 72 AseI AT'TA_AT Cuts at: 160 1032 4345 160 Size: 872 3313 1606 Fragments arranged by size: 3313 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 AvaI C'yCGr_G Cuts at: 1870 1943 3116 1870 Size: 73 1173 4545 Fragments arranged by size: 4545 1173 73 BamHI G'GATC_C Cuts at: 3121 3488 3121 Size: 367 5424 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2147 2702 3112 4567 618 Size: 325 217 627 360 555 410 1455 1842 Fragments arranged by size: 1842 1455 627 555 410 360 325 217 BanII G_rGCy'C Cuts at: 1391 2044 2364 3110 1391 Size: 653 320 746 4072 Fragments arranged by size: 4072 746 653 320 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4820 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3982 4016 1903 Size: 34 2045 34 3678 Fragments arranged by size: 3678 2045 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2441 3683 4893 2441 Size: 1242 1210 3339 Fragments arranged by size: 3339 1242 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5791 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4402 136 Size: 107 122 71 631 605 2730 1525 Fragments arranged by size: 2730 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5786 5786 Size: 5791 BlpI GC'TnA_GC Cuts at: 2043 3208 2043 Size: 1165 4626 Bme1580I G_kGCm'C Cuts at: 1790 3852 5008 1790 Size: 2062 1156 2573 Fragments arranged by size: 2573 2062 1156 BmgBI CAC'GTC Cuts at: 1318 2799 1318 Size: 1481 4310 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2047 2876 3522 Size: 416 53 83 399 24 630 829 646 Cuts at: 3522 4480 442 Size: 958 1753 Fragments arranged by size: 1753 958 829 646 630 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5791 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2201 4451 1239 Size: 962 2250 2579 Fragments arranged by size: 2579 2250 962 Bpu10I CC'TnA_GC Cuts at: 2370 2370 Size: 5791 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2121 3916 4781 5079 2121 Size: 1795 865 298 2833 Fragments arranged by size: 2833 1795 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2915 4454 882 Size: 617 1416 1539 2219 Fragments arranged by size: 2219 1539 1416 617 BsaAI yAC'GTr Cuts at: 493 1204 3582 493 Size: 711 2378 2702 Fragments arranged by size: 2702 2378 711 BsaBI GATnn'nnATC Cuts at: 5785 5785 Size: 5791 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2024 3598 3980 4615 Size: 53 83 186 936 491 1574 382 635 Cuts at: 4615 275 Size: 1451 Fragments arranged by size: 1574 1451 936 635 491 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2641 2924 4224 4622 4896 5043 5454 2641 Size: 283 1300 398 274 147 411 2978 Fragments arranged by size: 2978 1300 411 398 283 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2148 2178 1178 Size: 30 165 30 745 30 4791 Fragments arranged by size: 4791 745 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5791 BseYI C'CCAG_C Cuts at: 4994 5490 4994 Size: 496 5295 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5218 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3241 4002 5030 1540 Size: 275 186 1240 761 1028 2301 Fragments arranged by size: 2301 1240 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2364 2826 3852 3937 5008 2364 Size: 462 1026 85 1071 3147 Fragments arranged by size: 3147 1071 1026 462 85 BsmI GAATG_Cn' Cuts at: 3302 3395 3302 Size: 93 5698 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 2364 2826 3110 3852 3937 5008 Size: 399 254 320 462 284 742 85 1071 Cuts at: 5008 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 462 399 320 284 254 85 BspEI T'CCGG_A Cuts at: 5454 5454 Size: 5791 BspHI T'CATG_A Cuts at: 3680 5410 3680 Size: 1730 4061 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2224 3146 844 Size: 1380 922 3489 Fragments arranged by size: 3489 1380 922 BsrBI CCG'CTC Cuts at: 1394 1823 3678 1394 Size: 429 1855 3507 Fragments arranged by size: 3507 1855 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2942 4286 4460 65 Size: 1183 1694 1344 174 1396 Fragments arranged by size: 1694 1396 1344 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2641 4435 4622 1700 Size: 110 831 1794 187 2869 Fragments arranged by size: 2869 1794 831 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5791 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5791 BssSI C'ACGA_G Cuts at: 3851 4863 3851 Size: 1012 4779 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5791 BstBI TT'CG_AA Cuts at: 4595 4595 Size: 5791 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5791 BstXI CCAn_nnnn'nTGG Cuts at: 2669 2669 Size: 5791 BstYI r'GATC_y Cuts at: 1972 2493 3084 3121 3488 3873 3890 5342 5428 Size: 521 591 37 367 385 17 1452 86 Cuts at: 5428 5440 5786 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 591 521 385 367 346 86 37 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2706 513 Size: 552 939 702 3598 Fragments arranged by size: 3598 939 702 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5052 Fragments arranged by size: 5052 683 56 BtsI GCAGTG_nn' Cuts at: 3307 4101 4121 3307 Size: 794 20 4977 Fragments arranged by size: 4977 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5791 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2292 2327 529 Size: 35 1728 35 3993 Fragments arranged by size: 3993 1728 35 35 DraI TTT'AAA Cuts at: 3095 3095 Size: 5791 DraIII CAC_nnn'GTG Cuts at: 2847 2847 Size: 5791 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4798 809 Size: 507 3482 1802 Fragments arranged by size: 3482 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3238 4127 5756 Size: 54 1425 50 275 18 1408 889 1629 Cuts at: 5756 8 Size: 43 Fragments arranged by size: 1629 1425 1408 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3238 1537 Size: 275 1426 4090 Fragments arranged by size: 4090 1426 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2373 3721 1353 Size: 1020 1348 3423 Fragments arranged by size: 3423 1348 1020 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2894 4378 4752 4898 387 Size: 1064 138 1305 1484 374 146 1280 Fragments arranged by size: 1484 1305 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2362 2362 Size: 5791 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 2972 3854 4451 5238 5506 1239 Size: 393 569 771 882 597 787 268 1524 Fragments arranged by size: 1524 882 787 771 597 569 393 268 EcoRI G'AATT_C Cuts at: 3100 3478 3100 Size: 378 5413 FspI TGC'GCA Cuts at: 4297 4297 Size: 5791 HaeII r_GCGC'y Cuts at: 1678 2386 3064 4938 1678 Size: 708 678 1874 2531 Fragments arranged by size: 2531 1874 708 678 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2148 2180 2508 2540 4432 Size: 32 135 32 576 32 328 32 1892 Cuts at: 4432 4464 4506 4538 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1892 576 328 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2022 2503 3135 3381 669 Size: 988 281 84 481 632 246 3079 Fragments arranged by size: 3079 988 632 481 281 246 84 HindIII A'AGCT_T Cuts at: 748 748 Size: 5791 HpaI GTT'AAC Cuts at: 3381 3381 Size: 5791 KpnI G_GTAC'C Cuts at: 3116 3116 Size: 5791 MfeI C'AATT_G Cuts at: 2622 2763 3390 2622 Size: 141 627 5023 Fragments arranged by size: 5023 627 141 MluI A'CGCG_T Cuts at: 5667 5667 Size: 5791 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2422 4905 5089 1305 Size: 1117 2483 184 2007 Fragments arranged by size: 2483 2007 1117 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3969 Fragments arranged by size: 3969 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2807 2869 3749 4108 4267 518 Size: 648 1641 62 880 359 159 2042 Fragments arranged by size: 2042 1641 880 648 359 159 62 MspA1I CmG'CkG Cuts at: 1606 3207 3884 5032 5277 1606 Size: 1601 677 1148 245 2120 Fragments arranged by size: 2120 1601 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2706 513 Size: 552 939 702 3598 Fragments arranged by size: 3598 939 702 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5791 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5791 NotI GC'GGCC_GC Cuts at: 3238 3238 Size: 5791 NspI r_CATG'y Cuts at: 1132 1229 2033 2794 3149 4694 1132 Size: 97 804 761 355 1545 2229 Fragments arranged by size: 2229 1545 804 761 355 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5791 PciI A'CATG_T Cuts at: 4690 4690 Size: 5791 PflMI CCAn_nnn'nTGG Cuts at: 1108 2563 2593 1108 Size: 1455 30 4306 Fragments arranged by size: 4306 1455 30 PfoI T'CCnGG_A Cuts at: 2489 2489 Size: 5791 PmeI GTTT'AAAC Cuts at: 3095 3095 Size: 5791 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3860 3892 5373 5405 3860 Size: 32 1481 32 4246 Fragments arranged by size: 4246 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5791 PsiI TTA'TAA Cuts at: 3002 3045 3361 3002 Size: 43 316 5432 Fragments arranged by size: 5432 316 43 PstI C_TGCA'G Cuts at: 830 3143 830 Size: 2313 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5791 SacI G_AGCT'C Cuts at: 2364 2364 Size: 5791 SalI G'TCGA_C Cuts at: 1936 3133 1936 Size: 1197 4594 SapI GCTCTTCn'nnn_ Cuts at: 2373 2373 Size: 5791 SbfI CC_TGCA'GG Cuts at: 3143 3143 Size: 5791 ScaI AGT'ACT Cuts at: 4039 5751 4039 Size: 1712 4079 SfcI C'TryA_G Cuts at: 826 1045 3139 4274 4955 5146 826 Size: 219 2094 1135 681 191 1471 Fragments arranged by size: 2094 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 SgrAI Cr'CCGG_yG Cuts at: 1700 2641 1700 Size: 941 4850 SmaI CCC'GGG Cuts at: 1872 3118 1872 Size: 1246 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2136 2437 3895 4796 5058 820 Size: 197 926 193 301 1458 901 262 1553 Fragments arranged by size: 1553 1458 926 901 301 262 197 193 SnaBI TAC'GTA Cuts at: 493 3582 493 Size: 3089 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5791 SphI G_CATG'C Cuts at: 1132 3149 1132 Size: 2017 3774 SspI AAT'ATT Cuts at: 5 52 2913 3715 5 Size: 47 2861 802 2081 Fragments arranged by size: 2861 2081 802 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5791 StyI C'CwwG_G Cuts at: 513 1065 2004 2706 3230 513 Size: 552 939 702 524 3074 Fragments arranged by size: 3074 939 702 552 524 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3818 3835 3988 4173 4618 325 Size: 1255 2238 17 153 185 445 1498 Fragments arranged by size: 2238 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2248 4037 5749 Size: 274 80 33 51 1443 271 1789 1712 Cuts at: 5749 96 Size: 138 Fragments arranged by size: 1789 1712 1443 274 271 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5751 5783 5751 Size: 32 5759 Tth111I GACn'n_nGTC Cuts at: 1316 2162 1316 Size: 846 4945 XbaI T'CTAG_A Cuts at: 3127 3127 Size: 5791 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5791 XmaI C'CCGG_G Cuts at: 1870 3116 1870 Size: 1246 4545 XmnI GAAnn'nnTTC Cuts at: 3920 3920 Size: 5791 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3599 276 Size: 53 83 186 936 2065 2468 Fragments arranged by size: 2468 2065 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI