Restriction map for
pFN21AE3148
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AvaII AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BclI BcnI
BfaI BglI BglII BlnI BpuAI
BsaAI BsaBI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I Cfr13I ClaI CpoI
DraI DraII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeIII HindIII HpaI KasI KpnI
KspI MaeI MamI MluI MluNI
MroI MscI MspA1I NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI Sau96I
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 28 GTCTAC GT^[AC][GT]AC
AcyI 18 GACGCC G[GA]^CG[CT]C
AflII 432 CTTAAG C^TTAAG
AgeI 636 ACCGGT A^CCGGT
AspI 154 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 442 GGATAC GTATCC(6/5)
BfrI 432 CTTAAG C^TTAAG
BlpI 37 GCTCAGC GC^T[ACGT]AGC
BpmI 175 CTGGAG CTGGAG(16/14)
Bpu1102I 37 GCTCAGC GC^T[ACGT]AGC
BsaHI 18 GACGCC G[GA]^CG[CT]C
BsaI 916 GAGACC GGTCTC(1/5)
BsaJI 701 CCATGG C^C[ACGT][ACGT]GG
BspMI 228 GCAGGT ACCTGC(4/8)
BsrDI 938 CATTGC GCAATG(2/0)
BsrFI 636 ACCGGT [GA]^CCGG[CT]
BstXI 657 CCAGCTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 37 GCTCAGC GC^T[ACGT]AGC
Cfr10I 636 ACCGGT [GA]^CCGG[CT]
DraIII 837 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 701 CCATGG C^C[GA][CT]GG
EarI 373 GAAGAG CTCTTC(1/4)
EcoT14I 701 CCATGG C^C[AT][AT]GG
HgaI 18 GACGC GACGC(5/10)
Hin1I 18 GACGCC G[GA]^CG[CT]C
Ksp632I 373 GAAGAG CTCTTC(1/4)
MnlI 225 GAGG CCTC(7/6)
MseI 433 TTAA T^TAA
NcoI 701 CCATGG C^CATGG
PflFI 154 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 636 ACCGGT A^CCGGT
SacI 355 GAGCTC GAGCT^C
SapI 373 GAAGAGC GCTCTTC(1/4)
SgrAI 635 CACCGGTG C[GA]^CCGG[CT]G
SspI 906 AATATT AAT^ATT
StyI 701 CCATGG C^C[AT][AT]GG
Tru9I 433 TTAA T^TAA
Tth111I 154 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 49 CGCG CG^CG
64 CGCG
AlwNI 200 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
583 CAGATGCTG
AspHI 355 GAGCTC G[AT]GC[AT]^C
817 GTGCTC
BanI 142 GGTGCC G^G[CT][GA]CC
697 GGCACC
BanII 35 GGGCTC G[GA]GC[CT]^C
355 GAGCTC
BsaWI 636 ACCGGT [AT]^CCGG[AT]
919 ACCGGA
BsiHKAI 355 GAGCTC G[AT]GC[AT]^C
817 GTGCTC
BssKI 174 CCTGG ^CC[ACGT]GG
485 CCTGG
BstF5I 333 GGATG GGATG(2/0)
865 GGATG
BstNI 174 CCTGG CC^[AT]GG
485 CCTGG
BstUI 49 CGCG CG^CG
64 CGCG
BstYI 488 GGATCT [GA]^GATC[CT]
1079 AGATCC
Cac8I 50 GCGAGC GC[ACGT]^[ACGT]GC
588 GCTGGC
EcoRII 174 CCTGG ^CC[AT]GG
485 CCTGG
FokI 333 GGATG GGATG(9/13)
865 GGATG
HaeII 377 AGCGCC [GA]GCGC^[CT]
1055 GGCGCT
HapII 637 CCGG C^CGG
920 CCGG
HincII 15 GTTGAC GT[CT]^[GA]AC
496 GTCAAC
HindII 15 GTTGAC GT[CT]^[GA]AC
496 GTCAAC
HpaII 637 CCGG C^CGG
920 CCGG
MfeI 617 CAATTG C^AATTG
758 CAATTG
MflI 488 GGATCT [GA]^GATC[CT]
1079 AGATCC
MslI 798 CATTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
860 CACTGGGATG
MspI 637 CCGG C^CGG
920 CCGG
MunI 617 CAATTG C^AATTG
758 CAATTG
MvaI 174 CCTGG CC^[AT]GG
485 CCTGG
MvnI 49 CGCG CG^CG
64 CGCG
NspI 24 GCATGT [GA]CATG^[CT]
785 ACATGC
PflMI 552 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
582 CCAGATGCTGG
ScrFI 174 CCTGG CC^[ACGT]GG
485 CCTGG
SmlI 131 CTCAAG C^T[CT][GA]AG
432 CTTAAG
Van91I 552 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
582 CCAGATGCTGG
XhoII 488 GGATCT [GA]^GATC[CT]
1079 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BmyI BsiYI BslI
BsmAI Bsp1286I BsrI CfoI DdeI
DpnII Fnu4HI HhaI HinP1I HinfI
HphI ItaI MaeII MaeIII MboI
MboII MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI SfaNI TfiI
TseI Tsp45I Tsp509I TspRI