RGS14 ORF clone pFN21AE1990

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE1990

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Clone info.

Species

human

Clone Name

pFN21AE1990 Sequence (with vector) Restriction Map

Product ID

FHC10974

ORF Sequence

DNA sequence (1701 bp)
Protein sequence (567 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590533]

Gene

KIEE1990

Symbol

RGS14

Description

regulator of G-protein signaling 14

Original Clone ID

cs02890

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1701 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 567 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS43405.1 1.7e-185 99.8 RGS14 CCDS3368.1 2.3e-30 41.7 RGS12 CCDS3367.1 8.2e-30 41.9 RGS12 CCDS3366.1 8.6e-30 41.9 RGS12 CCDS41572.1 2.7e-17 49.0 RGS10 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000342 64 85 PR01301 Regulator of G protein signalling IPR000342 86 104 PR01301 Regulator of G protein signalling IPR000342 117 140 PR01301 Regulator of G protein signalling IPR000342 158 177 PR01301 Regulator of G protein signalling HMMPfam IPR000342 67 182 PF00615 Regulator of G protein signalling IPR003116 302 373 PF02196 Raf-like Ras-binding IPR003116 376 445 PF02196 Raf-like Ras-binding IPR003109 500 521 PF02188 GoLoco motif HMMSmart IPR000342 67 184 SM00315 Regulator of G protein signalling IPR003116 302 373 SM00455 Raf-like Ras-binding IPR003116 375 445 SM00455 Raf-like Ras-binding IPR003109 499 521 SM00390 GoLoco motif ProfileScan IPR000342 67 184 PS50132 Regulator of G protein signalling IPR003116 302 373 PS50898 Raf-like Ras-binding IPR003116 375 445 PS50898 Raf-like Ras-binding IPR003109 499 521 PS50877 GoLoco motif
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_005265851 567 602513 100.0 100.0 NP_006471 566 602513 99.8 100.0 XP_005265852 467 602513 100.0 81.5 NP_937870 799 602512 41.7 97.7 NP_002917 1376 602512 41.9 96.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1990 Download

>KIEE1990  1701 bp
ATGCCAGGGAAGCCCAAGCACCTGGGCGTCCCCAACGGGCGCATGGTTCTGGCTGTGTCA
GATGGAGAGCTGAGCAGCACGACGGGGCCCCAGGGCCAGGGCGAGGGCCGCGGCAGCTCT
CTCAGCATCCACAGCCTCCCCAGTGGTCCCAGCAGCCCCTTCCCAACCGAGGAGCAGCCT
GTGGCCAGCTGGGCCCTGTCCTTCGAGCGGCTGTTGCAGGACCCGCTGGGCCTGGCTTAC
TTCACTGAGTTCCTGAAGAAGGAGTTCAGCGCGGAAAACGTGACTTTCTGGAAGGCCTGC
GAGCGCTTCCAGCAGATCCCGGCCAGCGATACCCAGCAGCTAGCTCAGGAGGCCCGCAAC
ATCTACCAGGAGTTCCTGTCCAGCCAGGCGCTGAGCCCAGTGAACATCGACCGTCAGGCC
TGGCTTGGCGAGGAGGTGCTGGCCGAGCCCCGGCCGGACATGTTTCGGGCACAGCAGCTT
CAGATCTTCAACTTGATGAAGTTCGACAGCTATGCGCGCTTCGTCAAGTCCCCGCTGTAC
CGCGAGTGCCTGCTAGCCGAAGCCGAGGGACGCCCTCTGCGGGAACCTGGCTCCTCGCGC
CTCGGCAGCCCTGACGCCACGAGGAAGAAGCCGAAGCTGAAGCCCGGGAAGTCGCTGCCG
CTGGGTGTGGAGGAGTTGGGGCAGCTGCCACCCGTTGAGGGTCCTGGGGGCCGCCCTCTC
CGCAAGTCCTTCCGCCGGGAGCTGGGCGGGACTGCAAACGCCGCCTTGCGCCGAGAGTCT
CAGGGCTCCCTCAACTCCTCCGCCAGCCTGGACCTTGGCTTCCTAGCCTTCGTCAGCAGC
AAATCTGAGAGCCACCGGAAGAGCCTTGGGAGCACGGAGGGTGAAAGTGAAAGCCGGCCA
GGGAAGTACTGCTGTGTGTACCTGCCCGATGGCACAGCCTCCTTGGCCCTGGCCAGACCT
GGCCTCACCATCCGAGACATGCTGGCAGGGATCTGTGAGAAACGAGGCCTCTCTCTACCT
GACATCAAGGTCTACCTGGTGGGCAATGAACAGAAGGCCCTGGTCCTGGATCAGGACTGC
ACCGTGCTGGCGGATCAGGAAGTGCGGCTGGAAAACAGGATCACCTTCGAGCTGGAGCTG
ACGGCGCTGGAGCGCGTGGTACGAATCTCAGCCAAGCCCACCAAGCGGCTGCAGGAGGCG
CTGCAGCCCATTCTGGAGAAGCACGGCTTGAGCCCGCTAGAGGTGGTGCTGCACCGGCCA
GGCGAGAAACAGCCTCTGGATCTGGGGAAGCTAGTGAGCTCGGTGGCGGCCCAGAGACTG
GTTTTGGACACTCTTCCAGGTGTGAAGATCTCCAAAGCCCGTGACAAATCTCCCTGCCGC
AGCCAGGGCTGCCCACCTAGAACTCAGGATAAGGCCACCCATCCCCCTCCAGCGTCCCCC
AGTTCTCTGGTGAAGGTGCCCAGTAGTGCCACTGGAAAGCGGCAGACCTGTGACATCGAA
GGCCTGGTGGAGCTGCTGAACCGGGTGCAGAGCAGCGGGGCCCACGACCAGAGGGGCCTT
CTGAGGAAAGAGGACCTGGTACTTCCAGAATTTCTGCAGCTGCCCGCCCAAGGGCCCAGC
TCCGAGGAGACCCCACCACAGACCAAATCAGCAGCCCAGCCCATCGGGGGATCCTTGAAC
TCCACCACCGACTCAGCCCTC

ORF Protein

Cloned ORF protein sequence for pFN21AE1990 Download

>KIEE1990  567 aa
MPGKPKHLGVPNGRMVLAVSDGELSSTTGPQGQGEGRGSSLSIHSLPSGPSSPFPTEEQP
VASWALSFERLLQDPLGLAYFTEFLKKEFSAENVTFWKACERFQQIPASDTQQLAQEARN
IYQEFLSSQALSPVNIDRQAWLGEEVLAEPRPDMFRAQQLQIFNLMKFDSYARFVKSPLY
RECLLAEAEGRPLREPGSSRLGSPDATRKKPKLKPGKSLPLGVEELGQLPPVEGPGGRPL
RKSFRRELGGTANAALRRESQGSLNSSASLDLGFLAFVSSKSESHRKSLGSTEGESESRP
GKYCCVYLPDGTASLALARPGLTIRDMLAGICEKRGLSLPDIKVYLVGNEQKALVLDQDC
TVLADQEVRLENRITFELELTALERVVRISAKPTKRLQEALQPILEKHGLSPLEVVLHRP
GEKQPLDLGKLVSSVAAQRLVLDTLPGVKISKARDKSPCRSQGCPPRTQDKATHPPPASP
SSLVKVPSSATGKRQTCDIEGLVELLNRVQSSGAHDQRGLLRKEDLVLPEFLQLPAQGPS
SEETPPQTKSAAQPIGGSLNSTTDSAL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1990 Download

>pFN21AE1990  6406 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCAGGGAAGCCCAAGCACCTGGGCGTCCCCAA
CGGGCGCATGGTTCTGGCTGTGTCAGATGGAGAGCTGAGCAGCACGACGGGGCCCCAGGG
CCAGGGCGAGGGCCGCGGCAGCTCTCTCAGCATCCACAGCCTCCCCAGTGGTCCCAGCAG
CCCCTTCCCAACCGAGGAGCAGCCTGTGGCCAGCTGGGCCCTGTCCTTCGAGCGGCTGTT
GCAGGACCCGCTGGGCCTGGCTTACTTCACTGAGTTCCTGAAGAAGGAGTTCAGCGCGGA
AAACGTGACTTTCTGGAAGGCCTGCGAGCGCTTCCAGCAGATCCCGGCCAGCGATACCCA
GCAGCTAGCTCAGGAGGCCCGCAACATCTACCAGGAGTTCCTGTCCAGCCAGGCGCTGAG
CCCAGTGAACATCGACCGTCAGGCCTGGCTTGGCGAGGAGGTGCTGGCCGAGCCCCGGCC
GGACATGTTTCGGGCACAGCAGCTTCAGATCTTCAACTTGATGAAGTTCGACAGCTATGC
GCGCTTCGTCAAGTCCCCGCTGTACCGCGAGTGCCTGCTAGCCGAAGCCGAGGGACGCCC
TCTGCGGGAACCTGGCTCCTCGCGCCTCGGCAGCCCTGACGCCACGAGGAAGAAGCCGAA
GCTGAAGCCCGGGAAGTCGCTGCCGCTGGGTGTGGAGGAGTTGGGGCAGCTGCCACCCGT
TGAGGGTCCTGGGGGCCGCCCTCTCCGCAAGTCCTTCCGCCGGGAGCTGGGCGGGACTGC
AAACGCCGCCTTGCGCCGAGAGTCTCAGGGCTCCCTCAACTCCTCCGCCAGCCTGGACCT
TGGCTTCCTAGCCTTCGTCAGCAGCAAATCTGAGAGCCACCGGAAGAGCCTTGGGAGCAC
GGAGGGTGAAAGTGAAAGCCGGCCAGGGAAGTACTGCTGTGTGTACCTGCCCGATGGCAC
AGCCTCCTTGGCCCTGGCCAGACCTGGCCTCACCATCCGAGACATGCTGGCAGGGATCTG
TGAGAAACGAGGCCTCTCTCTACCTGACATCAAGGTCTACCTGGTGGGCAATGAACAGAA
GGCCCTGGTCCTGGATCAGGACTGCACCGTGCTGGCGGATCAGGAAGTGCGGCTGGAAAA
CAGGATCACCTTCGAGCTGGAGCTGACGGCGCTGGAGCGCGTGGTACGAATCTCAGCCAA
GCCCACCAAGCGGCTGCAGGAGGCGCTGCAGCCCATTCTGGAGAAGCACGGCTTGAGCCC
GCTAGAGGTGGTGCTGCACCGGCCAGGCGAGAAACAGCCTCTGGATCTGGGGAAGCTAGT
GAGCTCGGTGGCGGCCCAGAGACTGGTTTTGGACACTCTTCCAGGTGTGAAGATCTCCAA
AGCCCGTGACAAATCTCCCTGCCGCAGCCAGGGCTGCCCACCTAGAACTCAGGATAAGGC
CACCCATCCCCCTCCAGCGTCCCCCAGTTCTCTGGTGAAGGTGCCCAGTAGTGCCACTGG
AAAGCGGCAGACCTGTGACATCGAAGGCCTGGTGGAGCTGCTGAACCGGGTGCAGAGCAG
CGGGGCCCACGACCAGAGGGGCCTTCTGAGGAAAGAGGACCTGGTACTTCCAGAATTTCT
GCAGCTGCCCGCCCAAGGGCCCAGCTCCGAGGAGACCCCACCACAGACCAAATCAGCAGC
CCAGCCCATCGGGGGATCCTTGAACTCCACCACCGACTCAGCCCTCGTTTAAACGAATTC
GGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTG
CTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT
AACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAA
ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT
TTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT
ATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAA
TGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTA
ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT
CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA
AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGC
GGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAA
GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGC
CGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT
ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACT
GCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATA
CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTA
TTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT
AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT
AAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA
AATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACC
GACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC
GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACA
AAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC
TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC
CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT
TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTG
CTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTA
TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAA
AAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG
AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCC
TTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAAC
CATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTT
GCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGT
TTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGT
ACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAAC
CGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT