(Circular) (Six-base) MAPSORT of: pFN21AE1990.seq Check: 3332 from: 1 to: 6406 pFN21AE1990 6406 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4216 278 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3036 3749 1937 Size: 1099 713 4594 Fragments arranged by size: 4594 1099 713 Acc65I G'GTAC_C Cuts at: 3727 3727 Size: 6406 AclI AA'CG_TT Cuts at: 1563 4533 4906 1563 Size: 2970 373 3063 Fragments arranged by size: 3063 2970 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2279 2468 2663 4469 5853 6121 1632 Size: 647 189 195 1806 1384 268 1917 Fragments arranged by size: 1917 1806 1384 647 268 195 189 AfeI AGC'GCT Cuts at: 2309 2309 Size: 6406 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6209 AflIII A'CryG_T Cuts at: 1203 2463 5305 6282 1203 Size: 1260 2842 977 1327 Fragments arranged by size: 2842 1327 1260 977 AgeI A'CCGG_T Cuts at: 5237 5237 Size: 6406 AhdI GACnn_n'nnGTC Cuts at: 5135 5135 Size: 6406 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6406 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6406 AlwNI CAG_nnn'CTG Cuts at: 2395 3280 3322 4149 5721 2395 Size: 885 42 827 1572 3080 Fragments arranged by size: 3080 1572 885 827 42 ApaI G_GGCC'C Cuts at: 2094 2200 3547 3621 2094 Size: 106 1347 74 4879 Fragments arranged by size: 4879 1347 106 74 ApaLI G'TGCA_C Cuts at: 4463 5619 4463 Size: 1156 5250 ApoI r'AATT_y Cuts at: 1493 3593 3715 3941 4093 6202 1493 Size: 2100 122 226 152 2109 1697 Fragments arranged by size: 2109 2100 1697 226 152 122 AseI AT'TA_AT Cuts at: 160 1032 4960 160 Size: 872 3928 1606 Fragments arranged by size: 3928 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 AvaI C'yCGr_G Cuts at: 1870 1943 2648 3731 1870 Size: 73 705 1083 4545 Fragments arranged by size: 4545 1083 705 73 BamHI G'GATC_C Cuts at: 3674 3736 4103 3674 Size: 62 367 5977 Fragments arranged by size: 5977 367 62 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3460 3727 5182 618 Size: 325 217 627 1673 267 1455 1842 Fragments arranged by size: 1842 1673 1455 627 325 267 217 BanII G_rGCy'C Cuts at: 1391 2094 2200 2402 2454 2792 3239 3305 3547 Size: 703 106 202 52 338 447 66 242 Cuts at: 3547 3621 3725 1391 Size: 74 104 4072 Fragments arranged by size: 4072 703 447 338 242 202 106 104 74 66 52 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5435 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2498 2532 4597 4631 1903 Size: 34 50 34 477 34 2065 34 3678 Fragments arranged by size: 3678 2065 477 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4298 5508 4298 Size: 1210 5196 BclI T'GATC_A Cuts at: 1548 1548 Size: 6406 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2195 2948 5017 Size: 107 122 71 631 605 523 753 2069 Cuts at: 5017 136 Size: 1525 Fragments arranged by size: 2069 1525 753 631 605 523 122 107 71 BglII A'GATC_T Cuts at: 2487 3351 6401 2487 Size: 864 3050 2492 Fragments arranged by size: 3050 2492 864 BlpI GC'TnA_GC Cuts at: 2075 2396 3823 2075 Size: 321 1427 4658 Fragments arranged by size: 4658 1427 321 Bme1580I G_kGCm'C Cuts at: 1790 2094 2200 2476 3465 3547 3621 4467 5623 Size: 304 106 276 989 82 74 846 1156 Cuts at: 5623 1790 Size: 2573 Fragments arranged by size: 2573 1156 989 846 304 276 106 82 74 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6406 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2139 2396 3438 Size: 416 53 83 399 24 722 257 1042 Cuts at: 3438 3459 4137 5095 442 Size: 21 678 958 1753 Fragments arranged by size: 1753 1042 958 722 678 416 399 257 83 53 24 21 BmtI G_CTAG'C Cuts at: 1056 2348 2561 1056 Size: 1292 213 4901 Fragments arranged by size: 4901 1292 213 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3158 3173 3239 3417 5066 1239 Size: 1919 15 66 178 1649 2579 Fragments arranged by size: 2579 1919 1649 178 66 15 Bpu10I CC'TnA_GC Cuts at: 2349 2349 Size: 6406 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3253 4531 5396 5694 3253 Size: 1278 865 298 3965 Fragments arranged by size: 3965 1278 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3626 5069 882 Size: 617 2127 1443 2219 Fragments arranged by size: 2219 2127 1443 617 BsaAI yAC'GTr Cuts at: 493 1204 4197 493 Size: 711 2993 2702 Fragments arranged by size: 2993 2702 711 BsaBI GATnn'nnATC Cuts at: 6400 6400 Size: 6406 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2031 2575 2619 4213 Size: 53 83 186 936 498 544 44 1594 Cuts at: 4213 4595 5230 275 Size: 382 635 1451 Fragments arranged by size: 1594 1451 936 635 544 498 382 186 83 53 44 BsaWI w'CCGG_w Cuts at: 2859 4839 5237 5511 5658 6069 2859 Size: 1980 398 274 147 411 3196 Fragments arranged by size: 3196 1980 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2366 2396 1178 Size: 30 165 30 963 30 5188 Fragments arranged by size: 5188 963 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2189 2450 2587 2690 2791 3644 1186 Size: 1003 261 137 103 101 853 3948 Fragments arranged by size: 3948 1003 853 261 137 103 101 BseYI C'CCAG_C Cuts at: 2153 2193 2230 2337 2665 2746 3620 3660 5609 Size: 40 37 107 328 81 874 40 1949 Cuts at: 5609 6105 2153 Size: 496 2454 Fragments arranged by size: 2454 1949 874 496 328 107 81 40 40 37 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3067 3239 3551 1163 Size: 573 1331 172 312 4018 Fragments arranged by size: 4018 1331 573 312 172 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2416 2459 3856 4617 5645 1540 Size: 275 186 415 43 1397 761 1028 2301 Fragments arranged by size: 2301 1397 1028 761 415 275 186 43 BsiHKAI G_wGCw'C Cuts at: 2879 3305 4467 4552 5623 2879 Size: 426 1162 85 1071 3662 Fragments arranged by size: 3662 1162 1071 426 85 BsmI GAATG_Cn' Cuts at: 3917 4010 3917 Size: 93 6313 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2200 2402 2454 2476 2792 2879 Size: 399 304 106 202 52 22 316 87 Cuts at: 2879 3239 3305 3465 3547 3621 3725 4467 4552 Size: 360 66 160 82 74 104 742 85 Cuts at: 4552 5623 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 360 316 304 202 160 106 104 87 85 82 74 66 52 22 BspEI T'CCGG_A Cuts at: 6069 6069 Size: 6406 BspHI T'CATG_A Cuts at: 4295 6025 4295 Size: 1730 4676 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2934 3761 844 Size: 2090 827 3489 Fragments arranged by size: 3489 2090 827 BsrBI CCG'CTC Cuts at: 1394 1823 2212 4293 1394 Size: 429 389 2081 3507 Fragments arranged by size: 3507 2081 429 389 BsrDI GCAATG_nn' Cuts at: 65 1248 3055 4901 5075 65 Size: 1183 1807 1846 174 1396 Fragments arranged by size: 1846 1807 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2898 3258 5050 5237 1700 Size: 110 1088 360 1792 187 2869 Fragments arranged by size: 2869 1792 1088 360 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6406 BssHII G'CGCG_C Cuts at: 1925 2519 1925 Size: 594 5812 BssSI C'ACGA_G Cuts at: 2623 4466 5478 2623 Size: 1843 1012 3551 Fragments arranged by size: 3551 1843 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6406 BstBI TT'CG_AA Cuts at: 5210 5210 Size: 6406 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6406 BstXI CCAn_nnnn'nTGG Cuts at: 2026 3451 2026 Size: 1425 4981 BstYI r'GATC_y Cuts at: 1972 2319 2487 2994 3283 3351 3674 3736 4103 Size: 347 168 507 289 68 323 62 367 Cuts at: 4103 4488 4505 5957 6043 6055 6401 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 507 385 367 347 346 323 289 168 86 68 62 17 12 BtgI C'CryG_G Cuts at: 513 1065 2113 513 Size: 552 1048 4806 Fragments arranged by size: 4806 1048 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5667 Fragments arranged by size: 5667 683 56 BtsI GCAGTG_nn' Cuts at: 3922 4716 4736 3922 Size: 794 20 5592 Fragments arranged by size: 5592 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6406 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6371 DraI TTT'AAA Cuts at: 3710 3710 Size: 6406 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3032 5413 809 Size: 507 1716 2381 1802 Fragments arranged by size: 2381 1802 1716 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2187 2325 2445 Size: 54 1425 50 275 18 357 138 120 Cuts at: 2445 2456 2900 2955 3260 3853 4742 6371 8 Size: 11 444 55 305 593 889 1629 43 Fragments arranged by size: 1629 1425 889 593 444 357 305 275 138 120 55 54 50 43 18 11 EagI C'GGCC_G Cuts at: 1537 1812 2456 3853 1537 Size: 275 644 1397 4090 Fragments arranged by size: 4090 1397 644 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2858 3342 4336 1353 Size: 1505 484 994 3423 Fragments arranged by size: 3423 1505 994 484 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2726 2794 3110 4993 5367 5513 Size: 1064 138 1137 68 316 1883 374 146 Cuts at: 5513 387 Size: 1280 Fragments arranged by size: 1883 1280 1137 1064 374 316 146 138 68 EcoICRI GAG'CTC Cuts at: 3303 3303 Size: 6406 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2279 2468 2663 3158 3173 3239 3417 Size: 393 647 189 195 495 15 66 178 Cuts at: 3417 4469 5066 5853 6121 1239 Size: 1052 597 787 268 1524 Fragments arranged by size: 1524 1052 787 647 597 495 393 268 195 189 178 66 15 EcoNI CCTnn'n_nnAGG Cuts at: 2261 3074 2261 Size: 813 5593 EcoO109I rG'GnC_Cy Cuts at: 2090 2091 2197 2224 2705 3061 3543 3559 3577 Size: 1 106 27 481 356 482 16 18 Cuts at: 3577 3617 2090 Size: 40 4879 Fragments arranged by size: 4879 482 481 356 106 40 27 18 16 1 EcoRI G'AATT_C Cuts at: 3715 4093 3715 Size: 378 6028 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3247 3247 Size: 6406 FspI TGC'GCA Cuts at: 4912 4912 Size: 6406 HaeII r_GCGC'y Cuts at: 1678 2311 2396 3152 3206 5553 1678 Size: 633 85 756 54 2347 2531 Fragments arranged by size: 2531 2347 756 633 85 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2366 2398 2952 2984 5047 Size: 32 135 32 794 32 554 32 2063 Cuts at: 5047 5079 5121 5153 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2063 794 554 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3750 3996 669 Size: 988 281 1812 246 3079 Fragments arranged by size: 3079 1812 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6406 HpaI GTT'AAC Cuts at: 3996 3996 Size: 6406 KpnI G_GTAC'C Cuts at: 3731 3731 Size: 6406 MfeI C'AATT_G Cuts at: 4005 4005 Size: 6406 MluI A'CGCG_T Cuts at: 6282 6282 Size: 6406 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5520 5704 1305 Size: 4215 184 2007 Fragments arranged by size: 4215 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2189 2957 10 Size: 54 1425 343 357 768 3459 Fragments arranged by size: 3459 1425 768 357 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4364 4723 4882 518 Size: 648 3198 359 159 2042 Fragments arranged by size: 3198 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2115 2193 2230 2539 2665 2689 3540 3604 Size: 509 78 37 309 126 24 851 64 Cuts at: 3604 3822 4499 5647 5892 1606 Size: 218 677 1148 245 2120 Fragments arranged by size: 2120 1148 851 677 509 309 245 218 126 78 64 37 24 NaeI GCC'GGC Cuts at: 2900 2900 Size: 6406 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5854 NdeI CA'TA_TG Cuts at: 387 387 Size: 6406 NgoMIV G'CCGG_C Cuts at: 2898 2898 Size: 6406 NheI G'CTAG_C Cuts at: 1052 2344 2557 1052 Size: 1292 213 4901 Fragments arranged by size: 4901 1292 213 NotI GC'GGCC_GC Cuts at: 3853 3853 Size: 6406 NspI r_CATG'y Cuts at: 1132 1229 2467 2986 3764 5309 1132 Size: 97 1238 519 778 1545 2229 Fragments arranged by size: 2229 1545 1238 778 519 97 PasI CC'CwG_GG Cuts at: 1348 2095 1348 Size: 747 5659 PciI A'CATG_T Cuts at: 2463 5305 2463 Size: 2842 3564 PflMI CCAn_nnn'nTGG Cuts at: 1108 3322 1108 Size: 2214 4192 PfoI T'CCnGG_A Cuts at: 3069 3069 Size: 6406 PmeI GTTT'AAAC Cuts at: 3710 3710 Size: 6406 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4475 4507 5988 6020 4475 Size: 32 1481 32 4861 Fragments arranged by size: 4861 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2224 2705 3577 2224 Size: 481 872 5053 Fragments arranged by size: 5053 872 481 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6406 PsiI TTA'TAA Cuts at: 3976 3976 Size: 6406 PspOMI G'GGCC_C Cuts at: 2090 2196 3543 3617 2090 Size: 106 1347 74 4879 Fragments arranged by size: 4879 1347 106 74 PstI C_TGCA'G Cuts at: 830 3198 3210 3603 3758 830 Size: 2368 12 393 155 3478 Fragments arranged by size: 3478 2368 393 155 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6406 PvuII CAG'CTG Cuts at: 2193 2689 3604 2193 Size: 496 915 4995 Fragments arranged by size: 4995 915 496 SacI G_AGCT'C Cuts at: 3305 3305 Size: 6406 SacII CC_GC'GG Cuts at: 2116 2116 Size: 6406 SalI G'TCGA_C Cuts at: 1936 3748 1936 Size: 1812 4594 SapI GCTCTTCn'nnn_ Cuts at: 2858 2858 Size: 6406 SbfI CC_TGCA'GG Cuts at: 3758 3758 Size: 6406 ScaI AGT'ACT Cuts at: 2912 4654 6366 2912 Size: 1742 1712 2952 Fragments arranged by size: 2952 1742 1712 SexAI A'CCwGG_T Cuts at: 3039 3579 3039 Size: 540 5866 SfcI C'TryA_G Cuts at: 826 1045 3194 3206 3599 3754 4889 5570 5761 Size: 219 2149 12 393 155 1135 681 191 Cuts at: 5761 826 Size: 1471 Fragments arranged by size: 2149 1471 1135 681 393 219 191 155 12 SfiI GGCCn_nnn'nGGCC Cuts at: 2195 2195 Size: 6406 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6406 SmaI CCC'GGG Cuts at: 1872 2650 3733 1872 Size: 778 1083 4545 Fragments arranged by size: 4545 1083 778 SmlI C'TyrA_G Cuts at: 820 1017 1943 3232 4510 5411 5673 820 Size: 197 926 1289 1278 901 262 1553 Fragments arranged by size: 1553 1289 1278 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4197 493 Size: 3704 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6406 SphI G_CATG'C Cuts at: 1132 3764 1132 Size: 2632 3774 SspI AAT'ATT Cuts at: 5 52 4330 5 Size: 47 4278 2081 Fragments arranged by size: 4278 2081 47 StuI AGG'CCT Cuts at: 1517 2300 2423 3012 3507 1517 Size: 783 123 589 495 4416 Fragments arranged by size: 4416 783 589 495 123 StyI C'CwwG_G Cuts at: 513 1065 2818 2869 2946 3613 3845 513 Size: 552 1753 51 77 667 232 3074 Fragments arranged by size: 3074 1753 667 552 232 77 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2657 3437 4433 4450 4603 4788 5233 Size: 1255 1077 780 996 17 153 185 445 Cuts at: 5233 325 Size: 1498 Fragments arranged by size: 1498 1255 1077 996 780 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2910 4652 6364 Size: 274 80 33 51 1443 933 1742 1712 Cuts at: 6364 96 Size: 138 Fragments arranged by size: 1742 1712 1443 933 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6366 6398 6366 Size: 32 6374 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6406 XbaI T'CTAG_A Cuts at: 3742 3742 Size: 6406 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2044 2044 Size: 6406 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6406 XmaI C'CCGG_G Cuts at: 1870 2648 3731 1870 Size: 778 1083 4545 Fragments arranged by size: 4545 1083 778 XmnI GAAnn'nnTTC Cuts at: 2267 4535 2267 Size: 2268 4138 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4214 276 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SfoI SrfI SwaI