GDF9 ORF clone pFN21AE1918

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Halotag ORF Clone pFN21AE1918

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Clone info.

Species

human

Clone Name

pFN21AE1918 Sequence (with vector) Restriction Map

Product ID

FHC22275

ORF Sequence

DNA sequence (1362 bp)
Protein sequence (454 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590694]

Gene

KIEE1918

Symbol

GDF9

Alias : POF14

Description

growth differentiation factor 9, transcript variant 1

Original Clone ID

cs02818

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1362 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 454 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4162.1 1.5e-200 100.0 GDF9 CCDS75299.1 1.4e-159 100.0 GDF9 CCDS14334.1 2e-24 33.4 BMP15 CCDS4503.1 6.9e-14 28.4 BMP6 CCDS13099.1 2.2e-13 23.4 BMP2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001839 351 454 PF00019 Transforming growth factor-beta HMMSmart IPR001839 353 454 SM00204 Transforming growth factor-beta ProfileScan IPR001839 316 454 PS51362 Transforming growth factor-beta
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005251 454 601918 100.0 100.0 XP_011541610 454 601918 100.0 100.0 XP_005272014 366 601918 100.0 80.6 NP_001275757 366 601918 100.0 80.6 XP_011541611 366 601918 100.0 80.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1918 Download

>KIEE1918  1362 bp
ATGGCACGTCCCAACAAATTCCTCCTTTGGTTTTGCTGCTTTGCCTGGCTGTGTTTTCCT
ATTAGCCTTGGTTCTCAGGCTTCTGGGGGAGAAGCTCAGATTGCTGCTAGTGCTGAGTTG
GAATCTGGGGCTATGCCTTGGTCCTTGCTGCAGCATATAGATGAGAGAGACAGAGCTGGC
CTCCTTCCCGCGCTTTTCAAAGTTCTATCTGTTGGGCGAGGTGGGTCACCTAGGCTGCAG
CCAGACTCCAGAGCTTTGCACTACATGAAGAAGCTCTATAAGACATATGCTACCAAGGAA
GGGATTCCTAAATCCAATAGAAGTCACCTCTACAACACTGTTCGGCTCTTCACCCCCTGT
ACCCGGCACAAGCAGGCTCCTGGAGACCAGGTAACAGGAATCCTTCCATCAGTGGAACTG
CTATTTAACCTGGATCGCATTACTACCGTTGAACACTTACTCAAGTCAGTCTTGCTGTAC
AATATCAACAACTCAGTTTCTTTTTCCTCTGCTGTCAAATGTGTGTGCAATCTAATGATA
AAGGAGCCAAAGTCTTCTAGCAGGACTCTCGGCAGAGCTCCATACTCATTTACCTTTAAC
TCACAGTTTGAATTTGGAAAGAAACACAAATGGATTCAGATTGATGTGACCAGCCTCCTT
CAACCTTTAGTGGCCTCCAACAAGAGAAGTATTCACATGTCTATAAATTTTACTTGCATG
AAAGACCAGCTGGAGCATCCTTCAGCACAGAATGGTTTGTTTAACATGACTCTGGTGTCC
CCCTCACTGATCTTATATTTGAATGACACAAGTGCTCAGGCTTATCACAGCTGGTATTCC
CTTCACTATAAAAGGAGGCCTTCCCAGGGTCCTGACCAGGAGAGAAGTCTGTCTGCCTAT
CCTGTGGGAGAAGAGGCTGCTGAGGATGGGAGATCTTCCCATCACCGTCACCGCAGAGGT
CAGGAAACTGTCAGTTCTGAATTGAAGAAGCCCTTGGGCCCAGCTTCCTTCAATCTGAGT
GAATACTTCAGACAATTTCTTCTTCCCCAAAATGAGTGTGAGCTCCATGACTTTAGACTT
AGCTTTAGTCAGCTGAAGTGGGACAACTGGATTGTGGCTCCGCACAGGTACAACCCTCGA
TACTGTAAAGGGGACTGTCCAAGGGCAGTTGGACATCGGTATGGCTCTCCAGTTCACACC
ATGGTACAGAACATCATCTATGAGAAGCTGGACTCCTCAGTGCCAAGACCGTCATGTGTA
CCTGCCAAATACAGCCCCTTGAGTGTTTTGACCATTGAGCCCGATGGCTCAATTGCCTAT
AAAGAGTACGAAGATATGATAGCTACAAAGTGCACCTGTCGT

ORF Protein

Cloned ORF protein sequence for pFN21AE1918 Download

>KIEE1918  454 aa
MARPNKFLLWFCCFAWLCFPISLGSQASGGEAQIAASAELESGAMPWSLLQHIDERDRAG
LLPALFKVLSVGRGGSPRLQPDSRALHYMKKLYKTYATKEGIPKSNRSHLYNTVRLFTPC
TRHKQAPGDQVTGILPSVELLFNLDRITTVEHLLKSVLLYNINNSVSFSSAVKCVCNLMI
KEPKSSSRTLGRAPYSFTFNSQFEFGKKHKWIQIDVTSLLQPLVASNKRSIHMSINFTCM
KDQLEHPSAQNGLFNMTLVSPSLILYLNDTSAQAYHSWYSLHYKRRPSQGPDQERSLSAY
PVGEEAAEDGRSSHHRHRRGQETVSSELKKPLGPASFNLSEYFRQFLLPQNECELHDFRL
SFSQLKWDNWIVAPHRYNPRYCKGDCPRAVGHRYGSPVHTMVQNIIYEKLDSSVPRPSCV
PAKYSPLSVLTIEPDGSIAYKEYEDMIATKCTCR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1918 Download

>pFN21AE1918  6067 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCACGTCCCAACAAATTCCTCCTTTGGTTTTG
CTGCTTTGCCTGGCTGTGTTTTCCTATTAGCCTTGGTTCTCAGGCTTCTGGGGGAGAAGC
TCAGATTGCTGCTAGTGCTGAGTTGGAATCTGGGGCTATGCCTTGGTCCTTGCTGCAGCA
TATAGATGAGAGAGACAGAGCTGGCCTCCTTCCCGCGCTTTTCAAAGTTCTATCTGTTGG
GCGAGGTGGGTCACCTAGGCTGCAGCCAGACTCCAGAGCTTTGCACTACATGAAGAAGCT
CTATAAGACATATGCTACCAAGGAAGGGATTCCTAAATCCAATAGAAGTCACCTCTACAA
CACTGTTCGGCTCTTCACCCCCTGTACCCGGCACAAGCAGGCTCCTGGAGACCAGGTAAC
AGGAATCCTTCCATCAGTGGAACTGCTATTTAACCTGGATCGCATTACTACCGTTGAACA
CTTACTCAAGTCAGTCTTGCTGTACAATATCAACAACTCAGTTTCTTTTTCCTCTGCTGT
CAAATGTGTGTGCAATCTAATGATAAAGGAGCCAAAGTCTTCTAGCAGGACTCTCGGCAG
AGCTCCATACTCATTTACCTTTAACTCACAGTTTGAATTTGGAAAGAAACACAAATGGAT
TCAGATTGATGTGACCAGCCTCCTTCAACCTTTAGTGGCCTCCAACAAGAGAAGTATTCA
CATGTCTATAAATTTTACTTGCATGAAAGACCAGCTGGAGCATCCTTCAGCACAGAATGG
TTTGTTTAACATGACTCTGGTGTCCCCCTCACTGATCTTATATTTGAATGACACAAGTGC
TCAGGCTTATCACAGCTGGTATTCCCTTCACTATAAAAGGAGGCCTTCCCAGGGTCCTGA
CCAGGAGAGAAGTCTGTCTGCCTATCCTGTGGGAGAAGAGGCTGCTGAGGATGGGAGATC
TTCCCATCACCGTCACCGCAGAGGTCAGGAAACTGTCAGTTCTGAATTGAAGAAGCCCTT
GGGCCCAGCTTCCTTCAATCTGAGTGAATACTTCAGACAATTTCTTCTTCCCCAAAATGA
GTGTGAGCTCCATGACTTTAGACTTAGCTTTAGTCAGCTGAAGTGGGACAACTGGATTGT
GGCTCCGCACAGGTACAACCCTCGATACTGTAAAGGGGACTGTCCAAGGGCAGTTGGACA
TCGGTATGGCTCTCCAGTTCACACCATGGTACAGAACATCATCTATGAGAAGCTGGACTC
CTCAGTGCCAAGACCGTCATGTGTACCTGCCAAATACAGCCCCTTGAGTGTTTTGACCAT
TGAGCCCGATGGCTCAATTGCCTATAAAGAGTACGAAGATATGATAGCTACAAAGTGCAC
CTGTCGTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT