################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII BalI BamHI BanI BbeI BbrPI BciVI BclI BfrI BglI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccII 189 CGCG CG^CG AflIII 695 ACATGT A^C[GA][CT]GT Alw44I 1350 GTGCAC G^TGCAC AlwI 432 GGATC GGATC(4/5) ApaI 996 GGGCCC GGGCC^C ApaLI 1350 GTGCAC G^TGCAC Asp700I 298 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 229 CCTAGG C^CTAGG BbsI 552 GTCTTC GAAGAC(2/6) BbvCI 919 GCTGAGG CCTCAGC(-5/-2) BcgI 560 GCAGGACTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 362 CCCGG CC^[GC]GG BglII 931 AGATCT A^GATCT BlnI 229 CCTAGG C^CTAGG BpuAI 552 GTCTTC GAAGAC(2/6) BsaI 383 GAGACC GGTCTC(1/5) BseRI 1233 CTCCTC GAGGAG(10/8) Bsp1407I 476 TGTACA T^GTACA BspLU11I 695 ACATGT A^CATGT BspMI 1260 ACCTGC ACCTGC(4/8) BsrGI 476 TGTACA T^GTACA BstEII 224 GGTCACC G^GT[ACGT]ACC BstPI 224 GGTCACC G^GT[ACGT]ACC BstUI 189 CGCG CG^CG BstYI 931 AGATCT [GA]^GATC[CT] CfoI 190 GCGC GCG^C DraII 867 GGGTCCT [GA]G^G[ACGT]CC[CT] DrdI 768 GACTCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1199 CCATGG C^C[GA][CT]GG EcoO109I 867 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoO65I 224 GGTCACC G^GT[ACGT]ACC FauI 187 CCCGC CCCGC(4/6) HapII 363 CCGG C^CGG HhaI 190 GCGC GCG^C HinP1I 190 GCGC G^CGC HpaII 363 CCGG C^CGG MaeII 6 ACGT A^CGT MfeI 1310 CAATTG C^AATTG MflI 931 AGATCT [GA]^GATC[CT] MspI 363 CCGG C^CGG MunI 1310 CAATTG C^AATTG MvnI 189 CGCG CG^CG NciI 362 CCCGG CC^[GC]GG NcoI 1199 CCATGG C^CATGG NdeI 284 CATATG CA^TATG NspI 695 ACATGT [GA]CATG^[CT] PpuMI 867 GGGTCCT [GA]G^G[AT]CC[CT] SapI 345 GCTCTTC GCTCTTC(1/4) SexAI 386 ACCAGGT A^CC[AT]GGT SfaNI 735 GCATC GCATC(5/9) SspBI 476 TGTACA T^GTACA StuI 856 AGGCCT AGG^CCT TaqI 1137 TCGA T^CGA TthHB8I 1137 TCGA T^CGA XhoII 931 AGATCT [GA]^GATC[CT] XmnI 298 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AvaII 140 GGTCC G^G[AT]CC 868 GGTCC BsmAI 167 GAGAC GTCTC(1/5) 383 GAGAC BsrI 1106 ACTGG ACTGG(1/-1) 1189 CCAGT BstF5I 736 CATCC GGATG(2/0) 924 GGATG Cac8I 175 GCTGGC GC[ACGT]^[ACGT]GC 372 GCAGGC EarI 346 CTCTTC CTCTTC(1/4) 910 GAAGAG FokI 736 CATCC GGATG(9/13) 924 GGATG Ksp632I 346 CTCTTC CTCTTC(1/4) 910 GAAGAG MslI 154 CATATAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1174 CATCGGTATG MwoI 94 GCTCAGATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 103 GCTGCTAGTGC PstI 148 CTGCAG CTGCA^G 235 CTGCAG SacI 575 GAGCTC GAGCT^C 1060 GAGCTC SfcI 148 CTGCAG C^T[GA][CT]AG 235 CTGCAG SmlI 460 CTCAAG C^T[CT][GA]AG 1278 CTTGAG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AspHI BanII BbvI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssKI BstNI Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MspA1I MvaI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI