(Circular) (Six-base) MAPSORT of: pFN21AE1918.seq Check: 5243 from: 1 to: 6067 pFN21AE1918 6067 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3877 278 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3410 1937 Size: 1473 4594 Acc65I G'GTAC_C Cuts at: 3388 3388 Size: 6067 AclI AA'CG_TT Cuts at: 1563 4194 4567 1563 Size: 2631 373 3063 Fragments arranged by size: 3063 2631 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2730 3016 3119 4130 5514 5782 1632 Size: 1098 286 103 1011 1384 268 1917 Fragments arranged by size: 1917 1384 1098 1011 286 268 103 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5870 AflIII A'CryG_T Cuts at: 1203 2700 4966 5943 1203 Size: 1497 2266 977 1327 Fragments arranged by size: 2266 1497 1327 977 AgeI A'CCGG_T Cuts at: 4898 4898 Size: 6067 AhdI GACnn_n'nnGTC Cuts at: 4796 4796 Size: 6067 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6067 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6067 AlwNI CAG_nnn'CTG Cuts at: 3810 5382 3810 Size: 1572 4495 ApaI G_GGCC'C Cuts at: 3005 3005 Size: 6067 ApaLI G'TGCA_C Cuts at: 3355 4124 5280 3355 Size: 769 1156 4142 Fragments arranged by size: 4142 1156 769 ApoI r'AATT_y Cuts at: 1493 2021 2615 2710 3376 3602 3754 5863 1493 Size: 528 594 95 666 226 152 2109 1697 Fragments arranged by size: 2109 1697 666 594 528 226 152 95 AseI AT'TA_AT Cuts at: 160 1032 4621 160 Size: 872 3589 1606 Fragments arranged by size: 3589 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 AvaI C'yCGr_G Cuts at: 1870 1943 3392 1870 Size: 73 1449 4545 Fragments arranged by size: 4545 1449 73 AvrII C'CTAG_G Cuts at: 2234 2234 Size: 6067 BamHI G'GATC_C Cuts at: 3397 3764 3397 Size: 367 5700 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3388 4843 618 Size: 325 217 627 1601 1455 1842 Fragments arranged by size: 1842 1601 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2584 3005 3069 3306 3386 1391 Size: 1193 421 64 237 80 4072 Fragments arranged by size: 4072 1193 421 237 80 64 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2550 928 Size: 971 651 4445 Fragments arranged by size: 4445 971 651 BbvCI CC'TCA_GC Cuts at: 2925 2925 Size: 6067 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2554 2588 4258 4292 1903 Size: 34 617 34 1670 34 3678 Fragments arranged by size: 3678 1670 617 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3959 5169 3959 Size: 1210 4857 BclI T'GATC_A Cuts at: 1548 1548 Size: 6067 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4678 136 Size: 107 122 71 631 605 3006 1525 Fragments arranged by size: 3006 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2936 6062 2936 Size: 3126 2941 BlpI GC'TnA_GC Cuts at: 3484 3484 Size: 6067 Bme1580I G_kGCm'C Cuts at: 1790 3005 3359 4128 5284 1790 Size: 1215 354 769 1156 2573 Fragments arranged by size: 2573 1215 1156 769 354 BmgBI CAC'GTC Cuts at: 1318 2012 1318 Size: 694 5373 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3798 4756 442 Size: 416 53 83 399 24 2381 958 1753 Fragments arranged by size: 2381 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6067 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2201 2201 Size: 6067 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2236 2406 2756 3177 4727 1239 Size: 997 170 350 421 1550 2579 Fragments arranged by size: 2579 1550 997 421 350 170 Bpu10I CC'TnA_GC Cuts at: 2820 2925 2820 Size: 105 5962 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2450 3304 4192 5057 5355 2450 Size: 854 888 865 298 3162 Fragments arranged by size: 3162 888 865 854 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2382 4730 882 Size: 617 883 2348 2219 Fragments arranged by size: 2348 2219 883 617 BsaAI yAC'GTr Cuts at: 493 1204 3858 493 Size: 711 2654 2702 Fragments arranged by size: 2702 2654 711 BsaBI GATnn'nnATC Cuts at: 6061 6061 Size: 6067 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3874 4256 4891 275 Size: 53 83 186 936 2341 382 635 1451 Fragments arranged by size: 2341 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4500 4898 5172 5319 5730 4500 Size: 398 274 147 411 4837 Fragments arranged by size: 4837 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2379 2409 2540 2570 1178 Size: 30 165 30 976 30 131 30 4675 Fragments arranged by size: 4675 976 165 131 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3229 1186 Size: 2043 4024 BseYI C'CCAG_C Cuts at: 3004 5270 5766 3004 Size: 2266 496 3305 Fragments arranged by size: 3305 2266 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5494 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3517 4278 5306 1540 Size: 275 186 1516 761 1028 2301 Fragments arranged by size: 2301 1516 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2584 2821 3069 3359 4128 4213 5284 2584 Size: 237 248 290 769 85 1071 3367 Fragments arranged by size: 3367 1071 769 290 248 237 85 BsmI GAATG_Cn' Cuts at: 3578 3671 3578 Size: 93 5974 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2584 2821 3005 3069 3306 3359 3386 Size: 399 794 237 184 64 237 53 27 Cuts at: 3386 4128 4213 5284 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 794 742 399 237 237 184 85 64 53 27 BspEI T'CCGG_A Cuts at: 5730 5730 Size: 6067 BspHI T'CATG_A Cuts at: 3956 5686 3956 Size: 1730 4337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3274 3422 844 Size: 2430 148 3489 Fragments arranged by size: 3489 2430 148 BsrBI CCG'CTC Cuts at: 1394 1823 3954 1394 Size: 429 2131 3507 Fragments arranged by size: 3507 2131 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4562 4736 65 Size: 1183 3314 174 1396 Fragments arranged by size: 3314 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4711 4898 1700 Size: 110 2901 187 2869 Fragments arranged by size: 2901 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2481 96 Size: 2385 3682 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6067 BssSI C'ACGA_G Cuts at: 4127 5139 4127 Size: 1012 5055 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6067 BstBI TT'CG_AA Cuts at: 4871 4871 Size: 6067 BstEII G'GTnAC_C Cuts at: 1184 2229 1184 Size: 1045 5022 BstYI r'GATC_y Cuts at: 1972 2936 3397 3764 4149 4166 5618 5704 5716 Size: 964 461 367 385 17 1452 86 12 Cuts at: 5716 6062 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 964 461 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3204 513 Size: 552 939 1200 3376 Fragments arranged by size: 3376 1200 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5328 Fragments arranged by size: 5328 683 56 BtsI GCAGTG_nn' Cuts at: 3583 4377 4397 3583 Size: 794 20 5253 Fragments arranged by size: 5253 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6067 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6032 DraI TTT'AAA Cuts at: 3371 3371 Size: 6067 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2779 5074 809 Size: 507 1463 2295 1802 Fragments arranged by size: 2295 1802 1463 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3514 4403 6032 Size: 54 1425 50 275 18 1684 889 1629 Cuts at: 6032 8 Size: 43 Fragments arranged by size: 1684 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3514 1537 Size: 275 1702 4090 Fragments arranged by size: 4090 1702 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2357 2910 3997 1353 Size: 1004 553 1087 3423 Fragments arranged by size: 3423 1087 1004 553 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4654 5028 5174 387 Size: 1064 138 3065 374 146 1280 Fragments arranged by size: 3065 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2582 3067 2582 Size: 485 5582 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2236 2406 2730 2756 3016 3119 3177 Size: 393 604 170 324 26 260 103 58 Cuts at: 3177 4130 4727 5514 5782 1239 Size: 953 597 787 268 1524 Fragments arranged by size: 1524 953 787 604 597 393 324 268 260 170 103 58 26 EcoO109I rG'GnC_Cy Cuts at: 2873 2873 Size: 6067 EcoRI G'AATT_C Cuts at: 3376 3754 3376 Size: 378 5689 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2491 2880 3013 2491 Size: 389 133 5545 Fragments arranged by size: 5545 389 133 FspI TGC'GCA Cuts at: 4573 4573 Size: 6067 HaeII r_GCGC'y Cuts at: 1678 5214 1678 Size: 3536 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2111 2143 2540 2572 2921 Size: 32 135 32 539 32 397 32 349 Cuts at: 2921 2953 3106 3138 4708 4740 4782 4814 1373 Size: 32 153 32 1570 32 42 32 2626 Fragments arranged by size: 2626 1570 539 397 349 153 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3411 3657 669 Size: 988 281 1473 246 3079 Fragments arranged by size: 3079 1473 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6067 HpaI GTT'AAC Cuts at: 3657 3657 Size: 6067 KpnI G_GTAC'C Cuts at: 3392 3392 Size: 6067 MfeI C'AATT_G Cuts at: 3315 3666 3315 Size: 351 5716 MluI A'CGCG_T Cuts at: 5943 5943 Size: 6067 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2103 2706 3154 5181 5365 1305 Size: 798 603 448 2027 184 2007 Fragments arranged by size: 2027 2007 798 603 448 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4245 Fragments arranged by size: 4245 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2163 3183 4025 4384 4543 518 Size: 648 997 1020 842 359 159 2042 Fragments arranged by size: 2042 1020 997 842 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2734 2835 3097 3483 4160 5308 5553 1606 Size: 1128 101 262 386 677 1148 245 2120 Fragments arranged by size: 2120 1148 1128 677 386 262 245 101 NcoI C'CATG_G Cuts at: 513 1065 2004 3204 513 Size: 552 939 1200 3376 Fragments arranged by size: 3376 1200 939 552 NdeI CA'TA_TG Cuts at: 387 2290 387 Size: 1903 4164 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6067 NotI GC'GGCC_GC Cuts at: 3514 3514 Size: 6067 NspI r_CATG'y Cuts at: 1132 1229 2704 3425 4970 1132 Size: 97 1475 721 1545 2229 Fragments arranged by size: 2229 1545 1475 721 97 PasI CC'CwG_GG Cuts at: 1348 2869 1348 Size: 1521 4546 PciI A'CATG_T Cuts at: 2700 4966 2700 Size: 2266 3801 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6067 PfoI T'CCnGG_A Cuts at: 2383 2383 Size: 6067 PmeI GTTT'AAAC Cuts at: 3371 3371 Size: 6067 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2448 2480 4136 4168 5649 5681 2448 Size: 32 1656 32 1481 32 2834 Fragments arranged by size: 2834 1656 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2873 2873 Size: 6067 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6067 PsiI TTA'TAA Cuts at: 3637 3637 Size: 6067 PspOMI G'GGCC_C Cuts at: 3001 3001 Size: 6067 PstI C_TGCA'G Cuts at: 830 2157 2244 3419 830 Size: 1327 87 1175 3478 Fragments arranged by size: 3478 1327 1175 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6067 PvuII CAG'CTG Cuts at: 2734 2835 3097 2734 Size: 101 262 5704 Fragments arranged by size: 5704 262 101 SacI G_AGCT'C Cuts at: 2584 3069 2584 Size: 485 5582 SalI G'TCGA_C Cuts at: 1936 3409 1936 Size: 1473 4594 SapI GCTCTTCn'nnn_ Cuts at: 2357 2357 Size: 6067 SbfI CC_TGCA'GG Cuts at: 3419 3419 Size: 6067 ScaI AGT'ACT Cuts at: 4315 6027 4315 Size: 1712 4355 SexAI A'CCwGG_T Cuts at: 2391 2391 Size: 6067 SfcI C'TryA_G Cuts at: 826 1045 2153 2240 3415 4550 5231 5422 826 Size: 219 1108 87 1175 1135 681 191 1471 Fragments arranged by size: 1471 1175 1135 1108 681 219 191 87 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6067 SmaI CCC'GGG Cuts at: 1872 3394 1872 Size: 1522 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2465 3283 4171 5072 5334 820 Size: 197 926 522 818 888 901 262 1553 Fragments arranged by size: 1553 926 901 888 818 522 262 197 SnaBI TAC'GTA Cuts at: 493 3858 493 Size: 3365 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6067 SphI G_CATG'C Cuts at: 1132 3425 1132 Size: 2293 3774 SspI AAT'ATT Cuts at: 5 52 3991 5 Size: 47 3939 2081 Fragments arranged by size: 3939 2081 47 StuI AGG'CCT Cuts at: 1517 2863 1517 Size: 1346 4721 StyI C'CwwG_G Cuts at: 513 1065 2004 2071 2141 2234 2298 2997 3164 Size: 552 939 67 70 93 64 699 167 Cuts at: 3164 3204 3506 513 Size: 40 302 3074 Fragments arranged by size: 3074 939 699 552 302 167 93 70 67 64 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4094 4111 4264 4449 4894 325 Size: 1255 2514 17 153 185 445 1498 Fragments arranged by size: 2514 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2481 4313 6025 Size: 274 80 33 51 1443 504 1832 1712 Cuts at: 6025 96 Size: 138 Fragments arranged by size: 1832 1712 1443 504 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6027 6059 6027 Size: 32 6035 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6067 XbaI T'CTAG_A Cuts at: 3403 3403 Size: 6067 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6067 XmaI C'CCGG_G Cuts at: 1870 3392 1870 Size: 1522 4545 XmnI GAAnn'nnTTC Cuts at: 2307 4196 2307 Size: 1889 4178 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3875 276 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI