PRMT8 ORF clone pFN21AE1873

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Halotag ORF Clone pFN21AE1873

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Clone info.

Species

human

Clone Name

pFN21AE1873 Sequence (with vector) Restriction Map

Product ID

FHC10870

ORF Sequence

DNA sequence (1182 bp)
Protein sequence (394 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590500]

Gene

KIEE1873

Symbol

PRMT8

Alias : HRMT1L3, HRMT1L4

Description

protein arginine methyltransferase 8, transcript variant 1

Original Clone ID

cs02773

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1182 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 394 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8521.2 1.5e-149 100.0 PRMT8 CCDS58200.1 1.6e-140 99.2 PRMT8 CCDS42592.1 3.6e-109 79.2 PRMT1 CCDS46145.1 8.4e-109 80.1 PRMT1 CCDS44554.1 2.5e-55 49.5 PRMT3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR010456 108 184 PF06325 Ribosomal L11 methyltransferase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_062828 394 610086 100.0 100.0 NP_001243465 385 610086 99.2 94.7 NP_938074 353 602950 79.2 90.1 NP_001527 371 602950 80.1 87.8 XP_005258899 342 602950 81.5 86.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1873 Download

>KIEE1873  1182 bp
ATGGGCATGAAACACTCCTCCCGCTGCCTGCTCCTGAGGAGGAAAATGGCGGAGAACGCG
GCCGAGAGCACCGAGGTGAACAGCCCCCCCTCCCAGCCCCCCCAGCCCGTCGTCCCTGCT
AAGCCCGTGCAATGCGTCCATCATGTGTCCACTCAACCCAGCTGCCCAGGACGGGGCAAG
ATGTCCAAGCTGCTGAACCCAGAGGAGATGACCTCGAGAGATTATTACTTCGACTCCTAT
GCCCACTTTGGGATCCACGAGGAAATGCTGAAGGATGAGGTGCGGACTCTCACTTACCGG
AACTCCATGTACCACAACAAGCACGTGTTCAAGGACAAAGTGGTACTGGATGTGGGGAGT
GGTACTGGGATCCTTTCCATGTTCGCTGCCAAGGCAGGGGCCAAGAAGGTGTTTGGGATC
GAATGCTCCAGTATTTCTGACTACTCAGAGAAGATCATTAAGGCCAACCACTTGGACAAC
ATCATCACCATATTTAAGGGTAAAGTGGAAGAGGTGGAGCTGCCTGTGGAGAAGGTGGAC
ATCATCATCAGCGAGTGGATGGGCTACTGTCTGTTCTATGAGTCCATGCTCAACACGGTG
ATCTTTGCCAGGGACAAGTGGCTGAAACCTGGAGGGCTTATGTTTCCAGACCGGGCAGCT
TTGTACGTGGTAGCGATTGAAGACAGACAGTACAAGGACTTCAAAATCCACTGGTGGGAG
AATGTCTATGGCTTTGACATGACCTGCATCCGGGACGTGGCCATGAAGGAGCCTCTAGTG
GACATCGTGGATCCAAAGCAAGTGGTGACCAATGCCTGTTTGATAAAGGAGGTGGACATT
TACACAGTGAAGACGGAAGAGCTATCGTTCACATCTGCATTCTGCCTGCAGATACAGCGC
AACGACTACGTCCACGCCCTGGTCACCTATTTTAATATTGAATTTACCAAGTGCCACAAG
AAAATGGGGTTTTCCACAGCCCCTGATGCTCCCTACACCCACTGGAAGCAGACCGTCTTC
TACTTGGAAGATTACCTCACTGTCCGGAGGGGGGAGGAAATCTACGGGACCATATCCATG
AAGCCAAATGCCAAAAATGTGCGAGACCTCGATTTCACAGTAGACTTGGATTTTAAGGGA
CAGCTGTGTGAAACATCTGTATCTAATGACTACAAAATGCGT

ORF Protein

Cloned ORF protein sequence for pFN21AE1873 Download

>KIEE1873  394 aa
MGMKHSSRCLLLRRKMAENAAESTEVNSPPSQPPQPVVPAKPVQCVHHVSTQPSCPGRGK
MSKLLNPEEMTSRDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMYHNKHVFKDKVVLDVGS
GTGILSMFAAKAGAKKVFGIECSSISDYSEKIIKANHLDNIITIFKGKVEEVELPVEKVD
IIISEWMGYCLFYESMLNTVIFARDKWLKPGGLMFPDRAALYVVAIEDRQYKDFKIHWWE
NVYGFDMTCIRDVAMKEPLVDIVDPKQVVTNACLIKEVDIYTVKTEELSFTSAFCLQIQR
NDYVHALVTYFNIEFTKCHKKMGFSTAPDAPYTHWKQTVFYLEDYLTVRRGEEIYGTISM
KPNAKNVRDLDFTVDLDFKGQLCETSVSNDYKMR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1873 Download

>pFN21AE1873  5887 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGCATGAAACACTCCTCCCGCTGCCTGCTCCT
GAGGAGGAAAATGGCGGAGAACGCGGCCGAGAGCACCGAGGTGAACAGCCCCCCCTCCCA
GCCCCCCCAGCCCGTCGTCCCTGCTAAGCCCGTGCAATGCGTCCATCATGTGTCCACTCA
ACCCAGCTGCCCAGGACGGGGCAAGATGTCCAAGCTGCTGAACCCAGAGGAGATGACCTC
GAGAGATTATTACTTCGACTCCTATGCCCACTTTGGGATCCACGAGGAAATGCTGAAGGA
TGAGGTGCGGACTCTCACTTACCGGAACTCCATGTACCACAACAAGCACGTGTTCAAGGA
CAAAGTGGTACTGGATGTGGGGAGTGGTACTGGGATCCTTTCCATGTTCGCTGCCAAGGC
AGGGGCCAAGAAGGTGTTTGGGATCGAATGCTCCAGTATTTCTGACTACTCAGAGAAGAT
CATTAAGGCCAACCACTTGGACAACATCATCACCATATTTAAGGGTAAAGTGGAAGAGGT
GGAGCTGCCTGTGGAGAAGGTGGACATCATCATCAGCGAGTGGATGGGCTACTGTCTGTT
CTATGAGTCCATGCTCAACACGGTGATCTTTGCCAGGGACAAGTGGCTGAAACCTGGAGG
GCTTATGTTTCCAGACCGGGCAGCTTTGTACGTGGTAGCGATTGAAGACAGACAGTACAA
GGACTTCAAAATCCACTGGTGGGAGAATGTCTATGGCTTTGACATGACCTGCATCCGGGA
CGTGGCCATGAAGGAGCCTCTAGTGGACATCGTGGATCCAAAGCAAGTGGTGACCAATGC
CTGTTTGATAAAGGAGGTGGACATTTACACAGTGAAGACGGAAGAGCTATCGTTCACATC
TGCATTCTGCCTGCAGATACAGCGCAACGACTACGTCCACGCCCTGGTCACCTATTTTAA
TATTGAATTTACCAAGTGCCACAAGAAAATGGGGTTTTCCACAGCCCCTGATGCTCCCTA
CACCCACTGGAAGCAGACCGTCTTCTACTTGGAAGATTACCTCACTGTCCGGAGGGGGGA
GGAAATCTACGGGACCATATCCATGAAGCCAAATGCCAAAAATGTGCGAGACCTCGATTT
CACAGTAGACTTGGATTTTAAGGGACAGCTGTGTGAAACATCTGTATCTAATGACTACAA
AATGCGTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT