################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BanI BanII BbeI BbvCI BciVI BclI BfrI BglI BglII BlnI BsaBI BsaHI BsgI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI Eam1105I Eco47III EcoNI EcoO109I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TfiI XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AccI 1120 GTAGAC GT^[AC][GT]AC AccII 57 CGCG CG^CG AccIII 1043 TCCGGA T^CCGGA AcsI 940 GAATTT [GA]^AATT[CT] AflIII 323 ACGTGT A^C[GA][CT]GT AlwNI 977 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 33 CCTGAGG CC^T[ACGT]AGG ApoI 940 GAATTT [GA]^AATT[CT] AspHI 66 GAGCAC G[AT]GC[AT]^C AspI 904 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 213 CTCGAG C^[CT]CG[GA]G AvaII 1067 GGACC G^G[AT]CC BalI 758 TGGCCA TGG^CCA BbrPI 322 CACGTG CAC^GTG BcgI 231 CGACTCCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 775 CTAG C^TAG BlpI 118 GCTAAGC GC^T[ACGT]AGC BmyI 66 GAGCAC G[AGT]GC[ACT]^C Bpu1102I 118 GCTAAGC GC^T[ACGT]AGC BsaI 1103 GAGACC GGTCTC(1/5) BseAI 1043 TCCGGA T^CCGGA BsiEI 59 CGGCCG CG[GA][CT]^CG BsiHKAI 66 GAGCAC G[AT]GC[AT]^C BsmAI 1103 GAGAC GTCTC(1/5) BsoBI 213 CTCGAG C^[CT]CG[GA]G Bsp1286I 66 GAGCAC G[AGT]GC[ACT]^C BspEI 1043 TCCGGA T^CCGGA BspMI 742 ACCTGC ACCTGC(4/8) BsrDI 129 GCAATG GCAATG(2/0) BssSI 256 CACGAG CACGAG(-5/-1) BstUI 57 CGCG CG^CG BstXI 464 CCAACCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 33 CCTGAGG CC^T[ACGT]AGG CelII 118 GCTAAGC GC^T[ACGT]AGC CfoI 897 GCGC GCG^C EagI 59 CGGCCG C^GGCCG EclXI 59 CGGCCG C^GGCCG Eco52I 59 CGGCCG C^GGCCG Eco81I 33 CCTGAGG CC^T[ACGT]AGG EcoT14I 389 CCAAGG C^C[AT][AT]GG FauI 20 CCCGC CCCGC(4/6) HgaI 134 GCGTC GACGC(5/10) HhaI 897 GCGC GCG^C HinP1I 897 GCGC G^CGC MaeI 775 CTAG C^TAG MluNI 758 TGGCCA TGG^CCA MroI 1043 TCCGGA T^CCGGA MscI 758 TGGCCA TGG^CCA MvnI 57 CGCG CG^CG PaeR7I 213 CTCGAG C^TCGAG PflFI 904 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 912 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 322 CACGTG CAC^GTG PmlI 322 CACGTG CAC^GTG PstI 886 CTGCAG CTGCA^G SapI 856 GAAGAGC GCTCTTC(1/4) SfcI 886 CTGCAG C^T[GA][CT]AG SmlI 213 CTCGAG C^T[CT][GA]AG SspI 934 AATATT AAT^ATT StyI 389 CCAAGG C^C[AT][AT]GG Tsp509I 941 AATT ^AATT Tth111I 904 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 912 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 213 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BcnI 651 CCGGG CC^[GC]GG 750 CCGGG BpmI 426 CTCCAG CTGGAG(16/14) 629 CTGGAG BsaAI 322 CACGTG [CT]AC^GT[GA] 664 TACGTG BsaWI 296 ACCGGA [AT]^CCGG[AT] 1043 TCCGGA BsmI 421 GAATGC GAATGC(1/-1) 877 GCATTC BstEII 804 GGTGACC G^GT[ACGT]ACC 921 GGTCACC BstPI 804 GGTGACC G^GT[ACGT]ACC 921 GGTCACC Cac8I 26 GCCTGC GC[ACGT]^[ACGT]GC 884 GCCTGC Cfr13I 398 GGGCC G^G[ACGT]CC 1067 GGACC EaeI 59 CGGCCG [CT]^GGCC[GA] 758 TGGCCA EarI 508 GAAGAG CTCTTC(1/4) 856 GAAGAG EcoO65I 804 GGTGACC G^GT[ACGT]ACC 921 GGTCACC Ksp632I 508 GAAGAG CTCTTC(1/4) 856 GAAGAG MaeIII 805 GTGAC ^GT[ACGT]AC 922 GTCAC MslI 69 CACCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1071 CATATCCATG NciI 651 CCGGG CC^[GC]GG 750 CCGGG PvuII 159 CAGCTG CAG^CTG 1141 CAGCTG Sau96I 398 GGGCC G^G[ACGT]CC 1067 GGACC SfaNI 985 GATGC GCATC(5/9) 746 GCATC Tsp45I 805 GTGAC ^GT[GC]AC 922 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AfaI AluI AlwI BamHI BbsI BbvI BpuAI BsaJI BseRI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI BstYI DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI ScrFI TaqI Tru9I TseI TspRI TthHB8I XhoII