(Circular) (Six-base) MAPSORT of: pFN21AE1873.seq Check: 9841 from: 1 to: 5887 pFN21AE1873 5887 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3697 278 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3126 3230 1937 Size: 1189 104 4594 Fragments arranged by size: 4594 1189 104 Acc65I G'GTAC_C Cuts at: 3208 3208 Size: 5887 AclI AA'CG_TT Cuts at: 1563 4014 4387 1563 Size: 2451 373 3063 Fragments arranged by size: 3063 2451 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2294 3950 5334 5602 1632 Size: 662 1656 1384 268 1917 Fragments arranged by size: 1917 1656 1384 662 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5690 AflIII A'CryG_T Cuts at: 1203 2328 4786 5763 1203 Size: 1125 2458 977 1327 Fragments arranged by size: 2458 1327 1125 977 AgeI A'CCGG_T Cuts at: 4718 4718 Size: 5887 AhdI GACnn_n'nnGTC Cuts at: 4616 4616 Size: 5887 AleI CACnn'nnGTG Cuts at: 1166 2078 1166 Size: 912 4975 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5887 AlwNI CAG_nnn'CTG Cuts at: 2987 3630 5202 2987 Size: 643 1572 3672 Fragments arranged by size: 3672 1572 643 ApaLI G'TGCA_C Cuts at: 3944 5100 3944 Size: 1156 4731 ApoI r'AATT_y Cuts at: 1493 2945 3196 3422 3574 5683 1493 Size: 1452 251 226 152 2109 1697 Fragments arranged by size: 2109 1697 1452 251 226 152 AseI AT'TA_AT Cuts at: 160 1032 4441 160 Size: 872 3409 1606 Fragments arranged by size: 3409 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 AvaI C'yCGr_G Cuts at: 1870 1943 2218 3212 1870 Size: 73 275 994 4545 Fragments arranged by size: 4545 994 275 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3147 3180 3147 Size: 33 5854 BamHI G'GATC_C Cuts at: 2256 2373 2794 3217 3584 2256 Size: 117 421 423 367 4559 Fragments arranged by size: 4559 423 421 367 117 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3208 4663 618 Size: 325 217 627 1421 1455 1842 Fragments arranged by size: 1842 1455 1421 627 325 217 BanII G_rGCy'C Cuts at: 1391 3206 1391 Size: 1815 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2691 2861 3013 928 Size: 971 792 170 152 3802 Fragments arranged by size: 3802 971 792 170 152 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2225 2259 4078 4112 1903 Size: 34 288 34 1819 34 3678 Fragments arranged by size: 3678 1819 288 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3779 4989 3779 Size: 1210 4677 BclI T'GATC_A Cuts at: 1548 1548 Size: 5887 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4498 136 Size: 107 122 71 631 605 2826 1525 Fragments arranged by size: 2826 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5882 5882 Size: 5887 BlpI GC'TnA_GC Cuts at: 2124 3304 2124 Size: 1180 4707 Bme1580I G_kGCm'C Cuts at: 1790 3948 5104 1790 Size: 2158 1156 2573 Fragments arranged by size: 2573 2158 1156 BmgBI CAC'GTC Cuts at: 1318 2761 1318 Size: 1443 4444 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2379 3618 4576 Size: 416 53 83 399 24 962 1239 958 Cuts at: 4576 442 Size: 1753 Fragments arranged by size: 1753 1239 962 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5887 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2233 2233 Size: 5887 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2416 2655 4547 1239 Size: 1177 239 1892 2579 Fragments arranged by size: 2579 1892 1177 239 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4012 4877 5175 4012 Size: 865 298 4724 Fragments arranged by size: 4724 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3102 4550 882 Size: 617 1603 1448 2219 Fragments arranged by size: 2219 1603 1448 617 BsaAI yAC'GTr Cuts at: 493 1204 2329 2671 3678 493 Size: 711 1125 342 1007 2702 Fragments arranged by size: 2702 1125 1007 711 342 BsaBI GATnn'nnATC Cuts at: 5881 5881 Size: 5887 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3694 4076 4711 275 Size: 53 83 186 936 2161 382 635 1451 Fragments arranged by size: 2161 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2301 3048 4320 4718 4992 5139 5550 2301 Size: 747 1272 398 274 147 411 2638 Fragments arranged by size: 2638 1272 747 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2222 2252 2291 2321 1178 Size: 30 165 30 819 30 39 30 4744 Fragments arranged by size: 4744 819 165 39 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2011 2056 2222 1186 Size: 825 45 166 4851 Fragments arranged by size: 4851 825 166 45 BseYI C'CCAG_C Cuts at: 2097 2106 2162 5090 5586 2097 Size: 9 56 2928 496 2398 Fragments arranged by size: 2928 2398 496 56 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5314 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2067 3337 4098 5126 1540 Size: 275 186 66 1270 761 1028 2301 Fragments arranged by size: 2301 1270 1028 761 275 186 66 BsiHKAI G_wGCw'C Cuts at: 2075 3948 4033 5104 2075 Size: 1873 85 1071 2858 Fragments arranged by size: 2858 1873 1071 85 BsmI GAATG_Cn' Cuts at: 2432 2882 3398 3491 2432 Size: 450 516 93 4828 Fragments arranged by size: 4828 516 450 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 3206 3948 4033 5104 1391 Size: 399 285 1131 742 85 1071 2174 Fragments arranged by size: 2174 1131 1071 742 399 285 85 BspEI T'CCGG_A Cuts at: 3048 5550 3048 Size: 2502 3385 BspHI T'CATG_A Cuts at: 3776 5506 3776 Size: 1730 4157 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2756 3242 844 Size: 1912 486 3489 Fragments arranged by size: 3489 1912 486 BsrBI CCG'CTC Cuts at: 1394 1823 3774 1394 Size: 429 1951 3507 Fragments arranged by size: 3507 1951 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2141 4382 4556 65 Size: 1183 893 2241 174 1396 Fragments arranged by size: 2241 1396 1183 893 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4531 4718 1700 Size: 110 2721 187 2869 Fragments arranged by size: 2869 2721 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5887 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5887 BssSI C'ACGA_G Cuts at: 2261 3947 4959 2261 Size: 1686 1012 3189 Fragments arranged by size: 3189 1686 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5887 BstBI TT'CG_AA Cuts at: 4691 4691 Size: 5887 BstEII G'GTnAC_C Cuts at: 1184 2809 2926 1184 Size: 1625 117 4145 Fragments arranged by size: 4145 1625 117 BstXI CCAn_nnnn'nTGG Cuts at: 2476 2476 Size: 5887 BstYI r'GATC_y Cuts at: 1972 2256 2373 2794 3217 3584 3969 3986 5438 Size: 284 117 421 423 367 385 17 1452 Cuts at: 5438 5524 5536 5882 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 423 421 385 367 346 284 117 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2039 2039 Size: 5887 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4396 Fragments arranged by size: 4396 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5148 Fragments arranged by size: 5148 683 56 BtsI GCAGTG_nn' Cuts at: 3403 4197 4217 3403 Size: 794 20 5073 Fragments arranged by size: 5073 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5887 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2787 2822 529 Size: 35 2223 35 3594 Fragments arranged by size: 3594 2223 35 35 DraI TTT'AAA Cuts at: 3191 3191 Size: 5887 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4894 809 Size: 507 3578 1802 Fragments arranged by size: 3578 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 2763 3334 Size: 54 1425 50 275 18 234 699 571 Cuts at: 3334 4223 5852 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 699 571 275 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2064 3334 1537 Size: 275 252 1270 4090 Fragments arranged by size: 4090 1270 275 252 EarI CTCTTCn'nnn_ Cuts at: 1353 2508 2856 3817 1353 Size: 1155 348 961 3423 Fragments arranged by size: 3423 1155 961 348 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2069 4474 4848 4994 387 Size: 1064 138 480 2405 374 146 1280 Fragments arranged by size: 2405 1280 1064 480 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2294 2416 2655 3950 4547 5334 5602 Size: 393 662 122 239 1295 597 787 268 Cuts at: 5602 1239 Size: 1524 Fragments arranged by size: 1524 1295 787 662 597 393 268 239 122 EcoRI G'AATT_C Cuts at: 3196 3574 3196 Size: 378 5509 FspI TGC'GCA Cuts at: 4393 4393 Size: 5887 HaeII r_GCGC'y Cuts at: 1678 5034 1678 Size: 3356 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4528 4560 4602 4634 1373 Size: 32 135 32 2956 32 42 32 2626 Fragments arranged by size: 2956 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3231 3477 669 Size: 988 281 1293 246 3079 Fragments arranged by size: 3079 1293 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5887 HpaI GTT'AAC Cuts at: 3477 3477 Size: 5887 KpnI G_GTAC'C Cuts at: 3212 3212 Size: 5887 MfeI C'AATT_G Cuts at: 3486 3486 Size: 5887 MluI A'CGCG_T Cuts at: 5763 5763 Size: 5887 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5001 5185 1305 Size: 3696 184 2007 Fragments arranged by size: 3696 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2765 10 Size: 54 1425 343 933 3132 Fragments arranged by size: 3132 1425 933 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2078 3080 3845 4204 4363 518 Size: 648 843 69 1002 765 359 159 2042 Fragments arranged by size: 2042 1002 843 765 648 359 159 69 MspA1I CmG'CkG Cuts at: 1606 2028 2166 3148 3303 3980 5128 5373 1606 Size: 422 138 982 155 677 1148 245 2120 Fragments arranged by size: 2120 1148 982 677 422 245 155 138 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4396 Fragments arranged by size: 4396 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5887 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5887 NotI GC'GGCC_GC Cuts at: 3334 3334 Size: 5887 NspI r_CATG'y Cuts at: 1132 1229 3245 4790 1132 Size: 97 2016 1545 2229 Fragments arranged by size: 2229 2016 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5887 PciI A'CATG_T Cuts at: 4786 4786 Size: 5887 PflMI CCAn_nnn'nTGG Cuts at: 1108 2923 1108 Size: 1815 4072 PfoI T'CCnGG_A Cuts at: 2754 2754 Size: 5887 PmeI GTTT'AAAC Cuts at: 3191 3191 Size: 5887 PmlI CAC'GTG Cuts at: 2329 2329 Size: 5887 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2855 2887 3956 3988 5469 5501 2855 Size: 32 1069 32 1481 32 3241 Fragments arranged by size: 3241 1481 1069 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5887 PsiI TTA'TAA Cuts at: 3457 3457 Size: 5887 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2297 2329 2297 Size: 32 5855 PstI C_TGCA'G Cuts at: 830 2895 3239 830 Size: 2065 344 3478 Fragments arranged by size: 3478 2065 344 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5887 PvuII CAG'CTG Cuts at: 2166 3148 2166 Size: 982 4905 SalI G'TCGA_C Cuts at: 1936 3229 1936 Size: 1293 4594 SapI GCTCTTCn'nnn_ Cuts at: 2856 2856 Size: 5887 SbfI CC_TGCA'GG Cuts at: 3239 3239 Size: 5887 ScaI AGT'ACT Cuts at: 4135 5847 4135 Size: 1712 4175 SfcI C'TryA_G Cuts at: 826 1045 2891 3235 4370 5051 5242 826 Size: 219 1846 344 1135 681 191 1471 Fragments arranged by size: 1846 1471 1135 681 344 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5887 SmaI CCC'GGG Cuts at: 1872 3214 1872 Size: 1342 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2218 3991 4892 5154 820 Size: 197 926 275 1773 901 262 1553 Fragments arranged by size: 1773 1553 926 901 275 262 197 SnaBI TAC'GTA Cuts at: 493 3678 493 Size: 3185 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5887 SphI G_CATG'C Cuts at: 1132 3245 1132 Size: 2113 3774 SspI AAT'ATT Cuts at: 5 52 2941 3811 5 Size: 47 2889 870 2081 Fragments arranged by size: 2889 2081 870 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5887 StyI C'CwwG_G Cuts at: 513 1065 2004 2394 3326 513 Size: 552 939 390 932 3074 Fragments arranged by size: 3074 939 932 552 390 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3017 3914 3931 4084 4269 4714 325 Size: 1255 1437 897 17 153 185 445 1498 Fragments arranged by size: 1498 1437 1255 897 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2694 4133 5845 Size: 274 80 33 51 1443 717 1439 1712 Cuts at: 5845 96 Size: 138 Fragments arranged by size: 1712 1443 1439 717 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5847 5879 5847 Size: 32 5855 Tth111I GACn'n_nGTC Cuts at: 1316 2912 1316 Size: 1596 4291 XbaI T'CTAG_A Cuts at: 3223 3223 Size: 5887 XhoI C'TCGA_G Cuts at: 1943 2218 1943 Size: 275 5612 XmaI C'CCGG_G Cuts at: 1870 3212 1870 Size: 1342 4545 XmnI GAAnn'nnTTC Cuts at: 4016 4016 Size: 5887 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3695 276 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI