HYAL2 ORF clone pFN21AE1543

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE1543

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Clone info.

Species

human

Clone Name

pFN21AE1543 Sequence (with vector) Restriction Map

Product ID

FHC09736

ORF Sequence

DNA sequence (1419 bp)
Protein sequence (473 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590353]

Gene

KIEE1543

Symbol

HYAL2

Alias : LUCA2

Description

hyaluronoglucosaminidase 2, transcript variant 1

Original Clone ID

oc10066

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1419 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 473 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2818.1 4.3e-215 99.8 HYAL2 CCDS5789.1 3.8e-73 40.8 HYAL4 CCDS2816.1 1.6e-72 42.9 HYAL1 CCDS5791.1 1e-70 40.3 SPAM1 CCDS5790.1 1e-70 40.3 SPAM1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR018155 36 48 PR00846 Hyaluronidase IPR018155 96 110 PR00846 Hyaluronidase IPR018155 128 145 PR00846 Hyaluronidase IPR018155 186 211 PR00846 Hyaluronidase IPR018155 235 249 PR00846 Hyaluronidase IPR018155 340 353 PR00846 Hyaluronidase HMMPfam IPR018155 26 362 PF01630 Hyaluronidase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_005265582 473 603551 99.8 100.0 NP_003764 473 603551 99.8 100.0 XP_005265581 473 603551 99.8 100.0 NP_149348 473 603551 99.8 100.0 XP_016867400 481 604510 40.8 93.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1543 Download

>KIEE1543  1419 bp
ATGCGGGCAGGCCCAGGCCCCACCGTTACATTGGCCCTGGTGCTGGCGGTGGCATGGGCC
ATGGAGCTCAAGCCCACAGCACCACCCATCTTCACTGGCCGGCCCTTTGTGGTAGCGTGG
GACGTGCCCACACAGGACTGTGGCCCACGCCTCAAGGTGCCACTGGACCTGAATGCCTTT
GATGTGCAGGCCTCACCTAATGAGGGTTTTGTGAACCAGAATATTACCATCTTCTACCGC
GACCGTCTAGGCCTGTATCCACGCTTCGATTCTGCCGGAAGGTCTGTGCATGGTGGTGTG
CCACAGAATGTCAGCCTTTGGGCACACCGGAAGATGCTGCAGAAACGTGTGGAGCACTAC
ATTCGGACACAGGAGTCTGCGGGGCTGGCGGTCATCGACTGGGAGGACTGGCGACCTGTG
TGGGTGCGCAACTGGCAGGACAAAGATGTGTATCGCCGGTTATCACGCCAGCTAGTGGCC
AGTCGTCACCCTGACTGGCCTCCAGACCGCATAGTCAAACAGGCACAATATGAGTTTGAG
TTCGCAGCACAGCAGTTCATGCTGGAGACACTGCGTTATGTCAAGGCAGTGCGGCCCCGG
CACCTCTGGGGCTTCTACCTCTTTCCTGACTGCTACAATCATGATTATGTGCAGAACTGG
GAGAGCTACACAGGCCGCTGCCCTGATGTTGAGGTGGCCCGCAATGACCAGCTGGCCTGG
CTGTGGGCTGAGAGCACGGCCCTCTTCCCGTCTGTCTACCTGGACGAGACACTTGCTTCC
TCCCGCCATGGCCGCAACTTTGTGAGCTTCCGTGTTCAGGAGGCCCTTCGTGTGGCTCGC
ACCCACCATGCCAACCATGCACTCCCAGTCTACGTCTTCACACGACCCACCTACAGCCGC
AGGCTCACGGGGCTTAGTGAGATGGACCTCATCTCTACCATTGGCGAGAGTGCGGCCCTG
GGCGCAGCTGGTGTCATCCTCTGGGGTGACGCGGGGTACACCACAAGCACGGAGACCTGC
CAGTACCTCAAAGATTACCTGACACGGCTGCTGGTCCCCTACGTGGTCAATGTGTCCTGG
GCCACCCAATATTGCAGCCGGGCCCAGTGCCATGGCCATGGGCGCTGTGTGCGCCGCAAC
CCCAGTGCCAGTACCTTCCTGCATCTCAGCACCAACAGTTTCCGCCTAGTGCCTGGCCAT
GCACCTGGTGAACCCCAGCTGCGACCTGTGGGGGAGCTCAGTTGGGCCGACATTGACCAC
CTGCAGACACACTTCCGCTGCCAGTGCTACTTGGGCTGGAGTGGTGAGCAATGCCAGTGG
GACCATAGGCAGGCAGCTGGAGGTGCCAGCGAGGCCTGGGCTGGGTCCCACCTCACCAGT
CTGCTGGCTCTGGCAGCCCTGGCCTTTACCTGGACCTTG

ORF Protein

Cloned ORF protein sequence for pFN21AE1543 Download

>KIEE1543  473 aa
MRAGPGPTVTLALVLAVAWAMELKPTAPPIFTGRPFVVAWDVPTQDCGPRLKVPLDLNAF
DVQASPNEGFVNQNITIFYRDRLGLYPRFDSAGRSVHGGVPQNVSLWAHRKMLQKRVEHY
IRTQESAGLAVIDWEDWRPVWVRNWQDKDVYRRLSRQLVASRHPDWPPDRIVKQAQYEFE
FAAQQFMLETLRYVKAVRPRHLWGFYLFPDCYNHDYVQNWESYTGRCPDVEVARNDQLAW
LWAESTALFPSVYLDETLASSRHGRNFVSFRVQEALRVARTHHANHALPVYVFTRPTYSR
RLTGLSEMDLISTIGESAALGAAGVILWGDAGYTTSTETCQYLKDYLTRLLVPYVVNVSW
ATQYCSRAQCHGHGRCVRRNPSASTFLHLSTNSFRLVPGHAPGEPQLRPVGELSWADIDH
LQTHFRCQCYLGWSGEQCQWDHRQAAGGASEAWAGSHLTSLLALAALAFTWTL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1543 Download

>pFN21AE1543  6124 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCGGGCAGGCCCAGGCCCCACCGTTACATTGGC
CCTGGTGCTGGCGGTGGCATGGGCCATGGAGCTCAAGCCCACAGCACCACCCATCTTCAC
TGGCCGGCCCTTTGTGGTAGCGTGGGACGTGCCCACACAGGACTGTGGCCCACGCCTCAA
GGTGCCACTGGACCTGAATGCCTTTGATGTGCAGGCCTCACCTAATGAGGGTTTTGTGAA
CCAGAATATTACCATCTTCTACCGCGACCGTCTAGGCCTGTATCCACGCTTCGATTCTGC
CGGAAGGTCTGTGCATGGTGGTGTGCCACAGAATGTCAGCCTTTGGGCACACCGGAAGAT
GCTGCAGAAACGTGTGGAGCACTACATTCGGACACAGGAGTCTGCGGGGCTGGCGGTCAT
CGACTGGGAGGACTGGCGACCTGTGTGGGTGCGCAACTGGCAGGACAAAGATGTGTATCG
CCGGTTATCACGCCAGCTAGTGGCCAGTCGTCACCCTGACTGGCCTCCAGACCGCATAGT
CAAACAGGCACAATATGAGTTTGAGTTCGCAGCACAGCAGTTCATGCTGGAGACACTGCG
TTATGTCAAGGCAGTGCGGCCCCGGCACCTCTGGGGCTTCTACCTCTTTCCTGACTGCTA
CAATCATGATTATGTGCAGAACTGGGAGAGCTACACAGGCCGCTGCCCTGATGTTGAGGT
GGCCCGCAATGACCAGCTGGCCTGGCTGTGGGCTGAGAGCACGGCCCTCTTCCCGTCTGT
CTACCTGGACGAGACACTTGCTTCCTCCCGCCATGGCCGCAACTTTGTGAGCTTCCGTGT
TCAGGAGGCCCTTCGTGTGGCTCGCACCCACCATGCCAACCATGCACTCCCAGTCTACGT
CTTCACACGACCCACCTACAGCCGCAGGCTCACGGGGCTTAGTGAGATGGACCTCATCTC
TACCATTGGCGAGAGTGCGGCCCTGGGCGCAGCTGGTGTCATCCTCTGGGGTGACGCGGG
GTACACCACAAGCACGGAGACCTGCCAGTACCTCAAAGATTACCTGACACGGCTGCTGGT
CCCCTACGTGGTCAATGTGTCCTGGGCCACCCAATATTGCAGCCGGGCCCAGTGCCATGG
CCATGGGCGCTGTGTGCGCCGCAACCCCAGTGCCAGTACCTTCCTGCATCTCAGCACCAA
CAGTTTCCGCCTAGTGCCTGGCCATGCACCTGGTGAACCCCAGCTGCGACCTGTGGGGGA
GCTCAGTTGGGCCGACATTGACCACCTGCAGACACACTTCCGCTGCCAGTGCTACTTGGG
CTGGAGTGGTGAGCAATGCCAGTGGGACCATAGGCAGGCAGCTGGAGGTGCCAGCGAGGC
CTGGGCTGGGTCCCACCTCACCAGTCTGCTGGCTCTGGCAGCCCTGGCCTTTACCTGGAC
CTTGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT