MAPK4 ORF clone pFN21AE0982

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE0982

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Clone info.

Species

human

Clone Name

pFN21AE0982 Sequence (with vector) Restriction Map

Product ID

FHC29878

ORF Sequence

DNA sequence (1761 bp)
Protein sequence (587 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE0982

Symbol

MAPK4

Alias : ERK-4, ERK4, PRKM4, p63-MAPK, p63MAPK

Description

mitogen-activated protein kinase 4, transcript variant 1

Original Clone ID

cp01482

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1761 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 587 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS42437.1 2.6e-116 100.0 MAPK4 CCDS77189.1 1.6e-74 100.0 MAPK4 CCDS10147.1 1.1e-49 53.9 MAPK6 CCDS77188.1 1.7e-41 100.0 MAPK4 CCDS13795.1 1.2e-22 42.6 MAPK1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR008350 27 45 PR01771 ERK3/4 MAP kinase IPR008350 52 63 PR01771 ERK3/4 MAP kinase IPR008350 79 94 PR01771 ERK3/4 MAP kinase IPR008350 105 119 PR01771 ERK3/4 MAP kinase IPR008350 155 167 PR01771 ERK3/4 MAP kinase IPR008350 176 186 PR01771 ERK3/4 MAP kinase IPR008350 198 209 PR01771 ERK3/4 MAP kinase IPR008350 229 238 PR01771 ERK3/4 MAP kinase IPR008350 243 261 PR01771 ERK3/4 MAP kinase HMMPfam IPR017442 23 312 PF00069 Serine/threonine-protein kinase-like domain HMMSmart IPR002290 20 312 SM00220 Serine/threonine-protein kinase domain IPR020635 20 270 SM00219 Tyrosine-protein kinase ProfileScan IPR000719 20 312 PS50011 Protein kinase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011524377 587 176949 100.0 100.0 XP_011524378 587 176949 100.0 100.0 XP_016881328 587 176949 100.0 100.0 NP_002738 587 176949 100.0 100.0 XP_005258356 587 176949 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0982 Download

>KIEE0982  1761 bp
ATGGCTGAGAAGGGTGACTGCATCGCCAGTGTCTATGGGTATGACCTCGGTGGGCGCTTT
GTTGACTTCCAACCCCTGGGCTTCGGTGTCAATGGTTTGGTGCTGTCGGCCGTGGACAGC
CGGGCCTGCCGGAAGGTCGCTGTGAAGAAGATTGCCCTGAGCGATGCCCGCAGCATGAAG
CACGCGCTCCGAGAGATCAAGATCATTCGGCGCCTGGACCACGACAACATCGTCAAAGTG
TACGAGGTGCTCGGTCCCAAGGGCACTGACCTGCAGGGTGAGCTGTTCAAGTTCAGCGTG
GCGTACATCGTCCAGGAGTACATGGAGACCGACCTGGCACGCCTGCTGGAGCAGGGCACG
CTGGCAGAAGAGCATGCCAAGCTGTTCATGTACCAGCTGCTCCGCGGGCTCAAGTACATC
CACTCCGCCAACGTGCTGCACAGGGACCTGAAGCCCGCCAACATCTTCATCAGCACAGAG
GACCTCGTGCTCAAGATTGGGGATTTCGGGTTGGCAAGGATCGTTGATCAGCATTACTCC
CACAAGGGTTATCTGTCAGAAGGGTTGGTAACAAAGTGGTACCGTTCCCCACGACTGCTC
CTTTCCCCCAATAACTACACCAAAGCCATCGACATGTGGGCCGCCGGCTGCATCCTGGCT
GAGATGCTTACGGGGAGAATGCTCTTTGCTGGGGCCCATGAGCTGGAGCAGATGCAACTC
ATCCTGGAGACCATCCCTGTAATCCGGGAGGAAGACAAGGACGAGCTGCTCAGGGTGATG
CCTTCCTTTGTCAGCAGCACCTGGGAGGTGAAGAGGCCTCTGCGCAAGCTGCTCCCTGAA
GTGAACAGTGAAGCCATCGACTTTCTGGAGAAGATCCTGACCTTTAACCCCATGGATCGC
CTAACAGCTGAGATGGGGCTGCAACACCCCTACATGAGCCCATACTCGTGCCCTGAGGAC
GAGCCCACCTCACAACACCCCTTCCGCATTGAGGATGAGATCGACGACATCGTGCTGATG
GCCGCTAACCAGAGCCAGCTGTCCAACTGGGACACGTGCAGTTCCAGGTACCCTGTGAGC
CTGTCGTCGGACCTGGAGTGGCGGCCTGACCGGTGCCAGGACGCCAGCGAGGTACAGCGC
GACCCGCGCGCGGGTTCGGCGCCACTGGCTGAGGACGTGCAGGTGGACCCGCGCAAGGAC
TCGCACAGCAGCTCCGAGCGCTTCCTAGAGCAGTCGCACTCGTCCATGGAGCGCGCCTTC
GAGGCCGACTACGGGCGCTCCTGCGACTACAAGGTGGGGTCGCCGTCCTACCTGGACAAG
CTGCTGTGGCGCGACAACAAGCCGCACCACTACTCGGAGCCCAAGCTCATCCTGGACCTG
TCGCACTGGAAGCAGGCGGCCGGCGCGCCCCCCACGGCCACGGGGCTGGCGGACACGGGG
GCGCGCGAGGACGAGCCGGCCAGCCTCTTCCTGGAGATCGCGCAGTGGGTCAAGAGCACG
CAGGGCGGCCCAGAGCACGCCAGCCCGCCCGCCGACGACCCCGAGCGCCGCTTGTCTGCC
TCGCCCCCCGGCCGCCCGGCCCCGGTGGACGGCGGCGCCAGCCCCCAGTTCGACCTGGAC
GTGTTCATCTCCCGCGCCCTGAAGCTCTGCACCAAGCCCGAGGACCTGCCGGACAATAAA
CTGGGCGACCTCAATGGTGCGTGCATCCCCGAGCACCCTGGCGACCTCGTGCAGACCGAG
GCCTTCTCCAAAGAAAGGTGG

ORF Protein

Cloned ORF protein sequence for pFN21AE0982 Download

>KIEE0982  587 aa
MAEKGDCIASVYGYDLGGRFVDFQPLGFGVNGLVLSAVDSRACRKVAVKKIALSDARSMK
HALREIKIIRRLDHDNIVKVYEVLGPKGTDLQGELFKFSVAYIVQEYMETDLARLLEQGT
LAEEHAKLFMYQLLRGLKYIHSANVLHRDLKPANIFISTEDLVLKIGDFGLARIVDQHYS
HKGYLSEGLVTKWYRSPRLLLSPNNYTKAIDMWAAGCILAEMLTGRMLFAGAHELEQMQL
ILETIPVIREEDKDELLRVMPSFVSSTWEVKRPLRKLLPEVNSEAIDFLEKILTFNPMDR
LTAEMGLQHPYMSPYSCPEDEPTSQHPFRIEDEIDDIVLMAANQSQLSNWDTCSSRYPVS
LSSDLEWRPDRCQDASEVQRDPRAGSAPLAEDVQVDPRKDSHSSSERFLEQSHSSMERAF
EADYGRSCDYKVGSPSYLDKLLWRDNKPHHYSEPKLILDLSHWKQAAGAPPTATGLADTG
AREDEPASLFLEIAQWVKSTQGGPEHASPPADDPERRLSASPPGRPAPVDGGASPQFDLD
VFISRALKLCTKPEDLPDNKLGDLNGACIPEHPGDLVQTEAFSKERW

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0982 Download

>pFN21AE0982  6466 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTGAGAAGGGTGACTGCATCGCCAGTGTCTA
TGGGTATGACCTCGGTGGGCGCTTTGTTGACTTCCAACCCCTGGGCTTCGGTGTCAATGG
TTTGGTGCTGTCGGCCGTGGACAGCCGGGCCTGCCGGAAGGTCGCTGTGAAGAAGATTGC
CCTGAGCGATGCCCGCAGCATGAAGCACGCGCTCCGAGAGATCAAGATCATTCGGCGCCT
GGACCACGACAACATCGTCAAAGTGTACGAGGTGCTCGGTCCCAAGGGCACTGACCTGCA
GGGTGAGCTGTTCAAGTTCAGCGTGGCGTACATCGTCCAGGAGTACATGGAGACCGACCT
GGCACGCCTGCTGGAGCAGGGCACGCTGGCAGAAGAGCATGCCAAGCTGTTCATGTACCA
GCTGCTCCGCGGGCTCAAGTACATCCACTCCGCCAACGTGCTGCACAGGGACCTGAAGCC
CGCCAACATCTTCATCAGCACAGAGGACCTCGTGCTCAAGATTGGGGATTTCGGGTTGGC
AAGGATCGTTGATCAGCATTACTCCCACAAGGGTTATCTGTCAGAAGGGTTGGTAACAAA
GTGGTACCGTTCCCCACGACTGCTCCTTTCCCCCAATAACTACACCAAAGCCATCGACAT
GTGGGCCGCCGGCTGCATCCTGGCTGAGATGCTTACGGGGAGAATGCTCTTTGCTGGGGC
CCATGAGCTGGAGCAGATGCAACTCATCCTGGAGACCATCCCTGTAATCCGGGAGGAAGA
CAAGGACGAGCTGCTCAGGGTGATGCCTTCCTTTGTCAGCAGCACCTGGGAGGTGAAGAG
GCCTCTGCGCAAGCTGCTCCCTGAAGTGAACAGTGAAGCCATCGACTTTCTGGAGAAGAT
CCTGACCTTTAACCCCATGGATCGCCTAACAGCTGAGATGGGGCTGCAACACCCCTACAT
GAGCCCATACTCGTGCCCTGAGGACGAGCCCACCTCACAACACCCCTTCCGCATTGAGGA
TGAGATCGACGACATCGTGCTGATGGCCGCTAACCAGAGCCAGCTGTCCAACTGGGACAC
GTGCAGTTCCAGGTACCCTGTGAGCCTGTCGTCGGACCTGGAGTGGCGGCCTGACCGGTG
CCAGGACGCCAGCGAGGTACAGCGCGACCCGCGCGCGGGTTCGGCGCCACTGGCTGAGGA
CGTGCAGGTGGACCCGCGCAAGGACTCGCACAGCAGCTCCGAGCGCTTCCTAGAGCAGTC
GCACTCGTCCATGGAGCGCGCCTTCGAGGCCGACTACGGGCGCTCCTGCGACTACAAGGT
GGGGTCGCCGTCCTACCTGGACAAGCTGCTGTGGCGCGACAACAAGCCGCACCACTACTC
GGAGCCCAAGCTCATCCTGGACCTGTCGCACTGGAAGCAGGCGGCCGGCGCGCCCCCCAC
GGCCACGGGGCTGGCGGACACGGGGGCGCGCGAGGACGAGCCGGCCAGCCTCTTCCTGGA
GATCGCGCAGTGGGTCAAGAGCACGCAGGGCGGCCCAGAGCACGCCAGCCCGCCCGCCGA
CGACCCCGAGCGCCGCTTGTCTGCCTCGCCCCCCGGCCGCCCGGCCCCGGTGGACGGCGG
CGCCAGCCCCCAGTTCGACCTGGACGTGTTCATCTCCCGCGCCCTGAAGCTCTGCACCAA
GCCCGAGGACCTGCCGGACAATAAACTGGGCGACCTCAATGGTGCGTGCATCCCCGAGCA
CCCTGGCGACCTCGTGCAGACCGAGGCCTTCTCCAAAGAAAGGTGGGTTTAAACGAATTC
GGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTG
CTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT
AACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAA
ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT
TTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT
ATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAA
TGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTA
ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT
CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA
AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGC
GGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAA
GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGC
CGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT
ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACT
GCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATA
CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTA
TTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT
AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT
AAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA
AATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACC
GACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC
GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACA
AAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC
TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC
CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT
TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTG
CTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTA
TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAA
AAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG
AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCC
TTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAAC
CATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTT
GCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGT
TTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGT
ACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAAC
CGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT