################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AhdI Alw44I ApaLI ApoI AseI AsnI Asp700I AspEI AspI AvrII BalI BamHI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BseAI BseRI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII Eam1105I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI HindIII HpaI MfeI MluI MluNI MroI MscI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SspBI SspI SwaI TfiI Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AgeI 1109 ACCGGT A^CCGGT AlwNI 795 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 952 CCTGAGG CC^T[ACGT]AGG Aor51HI 1217 AGCGCT AGC^GCT ApaI 692 GGGCCC GGGCC^C AscI 1402 GGCGCGCC GG^CGCGCC AviII 821 TGCGCA TGC^GCA BbrPI 1053 CACGTG CAC^GTG BbsI 751 GAAGAC GAAGAC(2/6) BbvCI 1168 GCTGAGG CCTCAGC(-5/-2) BclI 525 TGATCA T^GATCA BfaI 1225 CTAG C^TAG BglI 1255 GCCTTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 751 GAAGAC GAAGAC(2/6) BsaAI 1053 CACGTG [CT]AC^GT[GA] BsaBI 195 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1109 ACCGGT [AT]^CCGG[AT] BsmI 677 GAATGC GAATGC(1/-1) BspLU11I 632 ACATGT A^CATGT BstXI 1418 CCACGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 872 AGATCC [GA]^GATC[CT] Bsu36I 952 CCTGAGG CC^T[ACGT]AGG DrdI 223 GACAACATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1217 AGCGCT AGC^GCT Eco81I 952 CCTGAGG CC^T[ACGT]AGG FbaI 525 TGATCA T^GATCA FspI 821 TGCGCA TGC^GCA HgaI 1120 GACGC GACGC(5/10) HincII 61 GTTGAC GT[CT]^[GA]AC HindII 61 GTTGAC GT[CT]^[GA]AC HinfI 1198 GACTC G^A[ACGT]TC KspI 402 CCGCGG CCGC^GG MaeI 1225 CTAG C^TAG MamI 195 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 872 AGATCC [GA]^GATC[CT] MseI 884 TTAA T^TAA PinAI 1109 ACCGGT A^CCGGT PleI 1198 GACTC GAGTC(4/5) PmaCI 1053 CACGTG CAC^GTG PmlI 1053 CACGTG CAC^GTG PstI 271 CTGCAG CTGCA^G SacII 402 CCGCGG CCGC^GG SapI 367 GAAGAGC GCTCTTC(1/4) SbfI 270 CCTGCAGG CCTGCA^GG SfcI 271 CTGCAG C^T[GA][CT]AG SphI 372 GCATGC GCATG^C Sse8387I 270 CCTGCAGG CCTGCA^GG Tru9I 884 TTAA T^TAA Tsp45I 14 GTGAC ^GT[GC]AC XhoII 872 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### Acc65I 578 GGTACC G^GTACC 1067 GGTACC Asp718I 578 GGTACC G^GTACC 1067 GGTACC BsaI 325 GAGACC GGTCTC(1/5) 727 GAGACC BsmAI 325 GAGAC GTCTC(1/5) 727 GAGAC KpnI 578 GGTACC GGTAC^C 1067 GGTACC MaeIII 14 GTGAC ^GT[ACGT]AC 568 GTAAC MslI 627 CATCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 941 CATACTCGTG NcoI 890 CCATGG C^CATGG 1244 CCATGG NspI 372 GCATGC [GA]CATG^[CT] 632 ACATGT PflMI 69 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 696 CCATGAGCTGG SmlI 409 CTCAAG C^T[CT][GA]AG 490 CTCAAG StuI 814 AGGCCT AGG^CCT 1739 AGGCCT Van91I 69 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 696 CCATGAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BmyI BpmI BsaHI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssHII BssKI BssSI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI KasI Ksp632I MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfoI StyI TaqI TseI TspRI TthHB8I XcmI