(Linear) (Six-base) MAPSORT of: KIEE0982.seq Check: 1111 from: 1 to: 1761 KIEE0982.orf With 182 enzymes: SgfI * March 22, 2012 15:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1170 1761 Size: 1170 591 Acc65I G'GTAC_C Cuts at: 0 578 1067 1761 Size: 578 489 694 Fragments arranged by size: 694 578 489 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 469 857 1660 1761 Size: 469 388 803 101 Fragments arranged by size: 803 469 388 101 AfeI AGC'GCT Cuts at: 0 1219 1761 Size: 1219 542 AflIII A'CryG_T Cuts at: 0 632 1052 1619 1761 Size: 632 420 567 142 Fragments arranged by size: 632 567 420 142 AgeI A'CCGG_T Cuts at: 0 1109 1761 Size: 1109 652 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 286 1761 Size: 286 1475 AlwNI CAG_nnn'CTG Cuts at: 0 800 1761 Size: 800 961 ApaI G_GGCC'C Cuts at: 0 696 1761 Size: 696 1065 AscI GG'CGCG_CC Cuts at: 0 1403 1761 Size: 1403 358 AvaI C'yCGr_G Cuts at: 0 1540 1657 1708 1761 Size: 1540 117 51 53 Fragments arranged by size: 1540 117 53 51 BanI G'GyrC_C Cuts at: 0 209 578 1067 1112 1158 1594 1761 Size: 209 369 489 45 46 436 167 Fragments arranged by size: 489 436 369 209 167 46 45 BanII G_rGCy'C Cuts at: 0 410 696 940 965 1361 1761 Size: 410 286 244 25 396 400 Fragments arranged by size: 410 400 396 286 244 25 BbeI G_GCGC'C Cuts at: 0 213 1162 1598 1761 Size: 213 949 436 163 Fragments arranged by size: 949 436 213 163 BbsI GAAGACnn'nnnn_ Cuts at: 0 758 1761 Size: 758 1003 BbvCI CC'TCA_GC Cuts at: 0 1169 1761 Size: 1169 592 BclI T'GATC_A Cuts at: 0 525 1761 Size: 525 1236 BglI GCCn_nnn'nGGC Cuts at: 0 1261 1761 Size: 1261 500 Bme1580I G_kGCm'C Cuts at: 0 265 358 696 952 1761 Size: 265 93 338 256 809 Fragments arranged by size: 809 338 265 256 93 BmgBI CAC'GTC Cuts at: 0 1176 1620 1761 Size: 1176 444 141 Fragments arranged by size: 1176 444 141 BmrI ACTGGGnnnn_n' Cuts at: 0 1056 1599 1690 1761 Size: 1056 543 91 71 Fragments arranged by size: 1056 543 91 71 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 984 1761 Size: 984 777 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 367 724 745 886 1114 1492 1761 Size: 367 357 21 141 228 378 269 Fragments arranged by size: 378 367 357 269 228 141 21 Bpu10I CC'TnA_GC Cuts at: 0 157 769 1169 1761 Size: 157 612 400 592 Fragments arranged by size: 612 592 400 157 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 394 475 1761 Size: 394 81 1286 Fragments arranged by size: 1286 394 81 BsaI GGTCTCn'nnnn_ Cuts at: 0 319 721 1761 Size: 319 402 1040 Fragments arranged by size: 1040 402 319 BsaAI yAC'GTr Cuts at: 0 1055 1761 Size: 1055 706 BsaBI GATnn'nnATC Cuts at: 0 199 1761 Size: 199 1562 BsaHI Gr'CG_yC Cuts at: 0 210 1121 1159 1595 1761 Size: 210 911 38 436 166 Fragments arranged by size: 911 436 210 166 38 BsaWI w'CCGG_w Cuts at: 0 1109 1761 Size: 1109 652 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 406 436 581 611 1195 1225 1261 1291 Size: 406 30 145 30 584 30 36 30 Cuts at: 1291 1761 Size: 470 Fragments arranged by size: 584 470 406 145 36 30 30 30 30 BseYI C'CCAG_C Cuts at: 0 688 1761 Size: 688 1073 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 421 1077 1198 1632 1750 1761 Size: 421 656 121 434 118 11 Fragments arranged by size: 656 434 421 121 118 11 BsiEI CG_ry'CG Cuts at: 0 110 1400 1572 1761 Size: 110 1290 172 189 Fragments arranged by size: 1290 189 172 110 BsiHKAI G_wGCw'C Cuts at: 0 251 491 1498 1517 1715 1761 Size: 251 240 1007 19 198 46 Fragments arranged by size: 1007 251 240 198 46 19 BsmI GAATG_Cn' Cuts at: 0 683 1761 Size: 683 1078 Bsp1286I G_dGCh'C Cuts at: 0 251 265 358 410 491 696 940 952 Size: 251 14 93 52 81 205 244 12 Cuts at: 952 965 1361 1498 1517 1715 1761 Size: 13 396 137 19 198 46 Fragments arranged by size: 396 251 244 205 198 137 93 81 52 46 19 14 13 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 278 1170 1673 1761 Size: 278 892 503 88 Fragments arranged by size: 892 503 278 88 BsrFI r'CCGG_y Cuts at: 0 643 1109 1399 1455 1761 Size: 643 466 290 56 306 Fragments arranged by size: 643 466 306 290 56 BssHII G'CGCG_C Cuts at: 0 1146 1251 1403 1441 1761 Size: 1146 105 152 38 320 Fragments arranged by size: 1146 320 152 105 38 BssSI C'ACGA_G Cuts at: 0 484 945 1726 1761 Size: 484 461 781 35 Fragments arranged by size: 781 484 461 35 BstXI CCAn_nnnn'nTGG Cuts at: 0 1425 1761 Size: 1425 336 BstYI r'GATC_y Cuts at: 0 872 1761 Size: 872 889 Bsu36I CC'TnA_GG Cuts at: 0 953 1761 Size: 953 808 BtgI C'CryG_G Cuts at: 0 110 402 890 1244 1412 1418 1761 Size: 110 292 488 354 168 6 343 Fragments arranged by size: 488 354 343 292 168 110 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 6 176 1761 Size: 6 170 1585 Fragments arranged by size: 1585 170 6 BtsI GCAGTG_nn' Cuts at: 0 1489 1761 Size: 1489 272 DrdI GACnn_nn'nnGTC Cuts at: 0 229 1761 Size: 229 1532 EaeI y'GGCC_r Cuts at: 0 107 1019 1397 1415 1457 1569 1761 Size: 107 912 378 18 42 112 192 Fragments arranged by size: 912 378 192 112 107 42 18 EagI C'GGCC_G Cuts at: 0 107 1397 1569 1761 Size: 107 1290 172 192 Fragments arranged by size: 1290 192 172 107 EarI CTCTTCn'nnn_ Cuts at: 0 362 805 1471 1761 Size: 362 443 666 290 Fragments arranged by size: 666 443 362 290 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 414 1444 1761 Size: 414 1030 317 Fragments arranged by size: 1030 414 317 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 367 469 724 745 857 886 1114 1492 Size: 367 102 255 21 112 29 228 378 Cuts at: 1492 1660 1761 Size: 168 101 Fragments arranged by size: 378 367 255 228 168 112 102 101 29 21 EcoO109I rG'GnC_Cy Cuts at: 0 444 480 692 1662 1761 Size: 444 36 212 970 99 Fragments arranged by size: 970 444 212 99 36 FspI TGC'GCA Cuts at: 0 823 1761 Size: 823 938 HaeII r_GCGC'y Cuts at: 0 57 213 1162 1221 1278 1548 1598 1761 Size: 57 156 949 59 57 270 50 163 Fragments arranged by size: 949 270 163 156 59 57 57 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 510 542 1761 Size: 510 32 1219 Fragments arranged by size: 1219 510 32 HincII GTy'rAC Cuts at: 0 63 1761 Size: 63 1698 KasI G'GCGC_C Cuts at: 0 209 1158 1594 1761 Size: 209 949 436 167 Fragments arranged by size: 949 436 209 167 KpnI G_GTAC'C Cuts at: 0 582 1071 1761 Size: 582 489 690 Fragments arranged by size: 690 582 489 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 93 1067 1761 Size: 93 974 694 Fragments arranged by size: 974 694 93 MslI CAynn'nnrTG Cuts at: 0 631 945 1761 Size: 631 314 816 Fragments arranged by size: 816 631 314 MspA1I CmG'CkG Cuts at: 0 396 404 907 1038 1761 Size: 396 8 503 131 723 Fragments arranged by size: 723 503 396 131 8 NaeI GCC'GGC Cuts at: 0 645 1401 1457 1761 Size: 645 756 56 304 Fragments arranged by size: 756 645 304 56 NarI GG'CG_CC Cuts at: 0 210 1159 1595 1761 Size: 210 949 436 166 Fragments arranged by size: 949 436 210 166 NcoI C'CATG_G Cuts at: 0 890 1244 1761 Size: 890 354 517 Fragments arranged by size: 890 517 354 NgoMIV G'CCGG_C Cuts at: 0 643 1399 1455 1761 Size: 643 756 56 306 Fragments arranged by size: 756 643 306 56 NspI r_CATG'y Cuts at: 0 376 636 1761 Size: 376 260 1125 Fragments arranged by size: 1125 376 260 PasI CC'CwG_GG Cuts at: 0 75 1761 Size: 75 1686 PciI A'CATG_T Cuts at: 0 632 1761 Size: 632 1129 PflMI CCAn_nnn'nTGG Cuts at: 0 75 702 1761 Size: 75 627 1059 Fragments arranged by size: 1059 627 75 PfoI T'CCnGG_A Cuts at: 0 311 722 743 1370 1469 1761 Size: 311 411 21 627 99 292 Fragments arranged by size: 627 411 311 292 99 21 PmlI CAC'GTG Cuts at: 0 1055 1761 Size: 1055 706 PpuMI rG'GwC_Cy Cuts at: 0 444 480 1662 1761 Size: 444 36 1182 99 Fragments arranged by size: 1182 444 99 36 PspOMI G'GGCC_C Cuts at: 0 692 1761 Size: 692 1069 PstI C_TGCA'G Cuts at: 0 275 1761 Size: 275 1486 PvuII CAG'CTG Cuts at: 0 396 907 1038 1761 Size: 396 511 131 723 Fragments arranged by size: 723 511 396 131 SacII CC_GC'GG Cuts at: 0 405 1761 Size: 405 1356 SapI GCTCTTCn'nnn_ Cuts at: 0 362 1761 Size: 362 1399 SbfI CC_TGCA'GG Cuts at: 0 275 1761 Size: 275 1486 SfcI C'TryA_G Cuts at: 0 271 1761 Size: 271 1490 SfoI GGC'GCC Cuts at: 0 211 1160 1596 1761 Size: 211 949 436 165 Fragments arranged by size: 949 436 211 165 SmlI C'TyrA_G Cuts at: 0 409 490 1761 Size: 409 81 1271 Fragments arranged by size: 1271 409 81 SphI G_CATG'C Cuts at: 0 376 1761 Size: 376 1385 StuI AGG'CCT Cuts at: 0 816 1741 1761 Size: 816 925 20 Fragments arranged by size: 925 816 20 StyI C'CwwG_G Cuts at: 0 257 890 1244 1761 Size: 257 633 354 517 Fragments arranged by size: 633 517 354 257 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 241 343 1750 1761 Size: 241 102 1407 11 Fragments arranged by size: 1407 241 102 11 TatI w'GTAC_w Cuts at: 0 317 413 1761 Size: 317 96 1348 Fragments arranged by size: 1348 317 96 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 581 613 1081 1113 1195 1227 1761 Size: 581 32 468 32 82 32 534 Fragments arranged by size: 581 534 468 82 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 861 1368 1467 1761 Size: 861 507 99 294 Fragments arranged by size: 861 507 294 99 Enzymes that do cut: AarI Acc65I AcuI AfeI AflIII AgeI AlfI AlwNI ApaI AscI AvaI BanI BanII BbeI BbsI BbvCI BclI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrFI BssHII BssSI BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I FspI HaeII Hin4I HincII KasI KpnI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacII SapI SbfI SfcI SfoI SmlI SphI StuI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AatII AccI AclI AflII AhdI AleI AloI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BfrBI BglII BlpI BmtI BseRI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HindIII HpaI MfeI MluI MscI NdeI NheI NotI NruI NsiI PacI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI