MRGPRE ORF clone pFN21AE0803

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Halotag ORF Clone pFN21AE0803

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Clone info.

Species

human

Clone Name

pFN21AE0803 Sequence (with vector) Restriction Map

Product ID

FHC11277

Flexi Clone PID

FXC11277

ORF Sequence

DNA sequence (936 bp)
Protein sequence (312 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB529239]

Gene

KIEE0803

Symbol

MRGPRE

Alias : GPR167, MGRF, MRGE

Description

MAS-related GPR, member E

Original Clone ID

cp01203

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 936 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 312 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS41603.2 4.7e-135 100.0 MRGPRE CCDS7830.1 1.3e-37 39.1 MRGPRX3 CCDS7846.1 4.2e-37 39.4 MRGPRX1 CCDS7847.1 1.4e-36 39.2 MRGPRX2 CCDS31625.1 4.1e-36 38.6 MRGPRD The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 26 50 PR00237 GPCR IPR000276 58 79 PR00237 GPCR IPR000276 103 125 PR00237 GPCR IPR000276 215 239 PR00237 GPCR IPR000276 251 277 PR00237 GPCR HMMPfam IPR000276 41 200 PF00001 GPCR ProfileScan IPR017452 41 269 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 24 NLIILSLTEGLGLGGLLGNGAVL 46 SECONDARY 23 2 60 IYLLDVACADLIFLGCHMVAIVP 82 PRIMARY 23 3 103 RFFCYIVGLSLLAAVSVEQCLAA 125 PRIMARY 23 4 142 CVCALTWALCLLLHLLLSGACTQ 164 PRIMARY 23 5 178 WLVAAVLLALLCCTMCGASLMLL 200 PRIMARY 23 6 214 GFPGLILLTVLLFLFCGLPFGIY 236 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001034254 312 607232 100.0 100.0 XP_011518184 322 607229 39.1 92.6 NP_473372 322 607229 39.1 92.6 NP_671732 322 607227 39.4 90.4 NP_001290544 330 607228 39.2 92.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0803 Download

>KIEE0803  936 bp
ATGATGGAGCCCAGAGAAGCTGGACAGCACGTGGGGGCCGCCAACGGCGCCCAGGAGGAT
GTGGCCTTCAACCTCATCATCCTGTCCCTCACCGAGGGGCTCGGCCTCGGTGGGCTGCTG
GGGAATGGGGCAGTCCTCTGGCTGCTCAGCTCCAATGTCTACAGAAACCCCTTCGCCATC
TACCTCCTGGACGTGGCCTGCGCGGATCTCATCTTCCTTGGCTGCCACATGGTGGCCATC
GTCCCCGACTTGCTGCAAGGCCGGCTGGACTTCCCGGGCTTCGTGCAGACCAGCCTGGCA
ACGCTGCGCTTCTTCTGCTACATCGTGGGCCTGAGTCTCCTGGCGGCCGTCAGCGTGGAG
CAGTGCCTGGCCGCCCTCTTCCCAGCCTGGTACTCGTGCCGCCGCCCACGCCACCTGACC
ACCTGTGTGTGCGCCCTCACCTGGGCCCTCTGCCTGCTGCTGCACCTGCTGCTCAGCGGC
GCCTGCACCCAGTTCTTCGGGGAGCCCAGCCGCCACTTGTGCCGGACGCTGTGGCTGGTG
GCAGCGGTGCTGCTGGCTCTGCTGTGTTGCACCATGTGTGGGGCCAGCCTTATGCTGCTG
CTGCGGGTGGAGCGAGGCCCCCAGCGGCCCCCACCCCGGGGCTTCCCTGGGCTCATCCTC
CTCACCGTCCTCCTCTTCCTCTTCTGCGGCCTGCCCTTCGGCATCTACTGGCTGTCCCGG
AACCTGCTCTGGTACATCCCCCACTACTTCTACCACTTCAGCTTCCTCATGGCCGCCGTG
CACTGCGCGGCCAAGCCCGTCGTCTACTTCTGCCTGGGCAGTGCCCAGGGCCGCAGGCTG
CCCCTCCGGCTGGTCCTCCAGCGAGCGCTGGGAGACGAGGCTGAGCTGGGGGCCGTCAGG
GAGACCTCCCGCCGGGGCCTGGTGGACATAGCAGCC

ORF Protein

Cloned ORF protein sequence for pFN21AE0803 Download

>KIEE0803  312 aa
MMEPREAGQHVGAANGAQEDVAFNLIILSLTEGLGLGGLLGNGAVLWLLSSNVYRNPFAI
YLLDVACADLIFLGCHMVAIVPDLLQGRLDFPGFVQTSLATLRFFCYIVGLSLLAAVSVE
QCLAALFPAWYSCRRPRHLTTCVCALTWALCLLLHLLLSGACTQFFGEPSRHLCRTLWLV
AAVLLALLCCTMCGASLMLLLRVERGPQRPPPRGFPGLILLTVLLFLFCGLPFGIYWLSR
NLLWYIPHYFYHFSFLMAAVHCAAKPVVYFCLGSAQGRRLPLRLVLQRALGDEAELGAVR
ETSRRGLVDIAA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0803 Download

>pFN21AE0803  5641 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGATGGAGCCCAGAGAAGCTGGACAGCACGTGGG
GGCCGCCAACGGCGCCCAGGAGGATGTGGCCTTCAACCTCATCATCCTGTCCCTCACCGA
GGGGCTCGGCCTCGGTGGGCTGCTGGGGAATGGGGCAGTCCTCTGGCTGCTCAGCTCCAA
TGTCTACAGAAACCCCTTCGCCATCTACCTCCTGGACGTGGCCTGCGCGGATCTCATCTT
CCTTGGCTGCCACATGGTGGCCATCGTCCCCGACTTGCTGCAAGGCCGGCTGGACTTCCC
GGGCTTCGTGCAGACCAGCCTGGCAACGCTGCGCTTCTTCTGCTACATCGTGGGCCTGAG
TCTCCTGGCGGCCGTCAGCGTGGAGCAGTGCCTGGCCGCCCTCTTCCCAGCCTGGTACTC
GTGCCGCCGCCCACGCCACCTGACCACCTGTGTGTGCGCCCTCACCTGGGCCCTCTGCCT
GCTGCTGCACCTGCTGCTCAGCGGCGCCTGCACCCAGTTCTTCGGGGAGCCCAGCCGCCA
CTTGTGCCGGACGCTGTGGCTGGTGGCAGCGGTGCTGCTGGCTCTGCTGTGTTGCACCAT
GTGTGGGGCCAGCCTTATGCTGCTGCTGCGGGTGGAGCGAGGCCCCCAGCGGCCCCCACC
CCGGGGCTTCCCTGGGCTCATCCTCCTCACCGTCCTCCTCTTCCTCTTCTGCGGCCTGCC
CTTCGGCATCTACTGGCTGTCCCGGAACCTGCTCTGGTACATCCCCCACTACTTCTACCA
CTTCAGCTTCCTCATGGCCGCCGTGCACTGCGCGGCCAAGCCCGTCGTCTACTTCTGCCT
GGGCAGTGCCCAGGGCCGCAGGCTGCCCCTCCGGCTGGTCCTCCAGCGAGCGCTGGGAGA
CGAGGCTGAGCTGGGGGCCGTCAGGGAGACCTCCCGCCGGGGCCTGGTGGACATAGCAGC
CGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
T