(Circular) (Six-base) MAPSORT of: pFN21AE0803.seq Check: 3381 from: 1 to: 5641 pFN21AE0803 5641 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2478 2478 Size: 5641 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3451 278 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2163 2808 2984 1937 Size: 226 645 176 4594 Fragments arranged by size: 4594 645 226 176 Acc65I G'GTAC_C Cuts at: 2962 2962 Size: 5641 AclI AA'CG_TT Cuts at: 1563 3768 4141 1563 Size: 2205 373 3063 Fragments arranged by size: 3063 2205 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2746 3704 5088 5356 1632 Size: 1114 958 1384 268 1917 Fragments arranged by size: 1917 1384 1114 958 268 AfeI AGC'GCT Cuts at: 2871 2871 Size: 5641 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5444 AflIII A'CryG_T Cuts at: 1203 4540 5517 1203 Size: 3337 977 1327 Fragments arranged by size: 3337 1327 977 AgeI A'CCGG_T Cuts at: 4472 4472 Size: 5641 AhdI GACnn_n'nnGTC Cuts at: 4370 4370 Size: 5641 AleI CACnn'nnGTG Cuts at: 1166 2429 1166 Size: 1263 4378 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5641 AlwNI CAG_nnn'CTG Cuts at: 2371 3384 4956 2371 Size: 1013 1572 3056 Fragments arranged by size: 3056 1572 1013 ApaI G_GGCC'C Cuts at: 2452 2452 Size: 5641 ApaLI G'TGCA_C Cuts at: 2783 3698 4854 2783 Size: 915 1156 3570 Fragments arranged by size: 3570 1156 915 ApoI r'AATT_y Cuts at: 1493 2950 3176 3328 5437 1493 Size: 1457 226 152 2109 1697 Fragments arranged by size: 2109 1697 1457 226 152 AseI AT'TA_AT Cuts at: 160 1032 4195 160 Size: 872 3163 1606 Fragments arranged by size: 3163 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 AvaI C'yCGr_G Cuts at: 1870 1943 2278 2640 2966 1870 Size: 73 335 362 326 4545 Fragments arranged by size: 4545 362 335 326 73 BamHI G'GATC_C Cuts at: 2971 3338 2971 Size: 367 5274 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2051 2483 2962 4417 618 Size: 325 217 627 264 432 479 1455 1842 Fragments arranged by size: 1842 1455 627 479 432 325 264 217 BanII G_rGCy'C Cuts at: 1391 2016 2106 2452 2511 2658 2960 1391 Size: 625 90 346 59 147 302 4072 Fragments arranged by size: 4072 625 346 302 147 90 59 BbeI G_GCGC'C Cuts at: 2055 2487 2055 Size: 432 5209 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4670 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3832 3866 1903 Size: 34 1895 34 3678 Fragments arranged by size: 3678 1895 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3533 4743 3533 Size: 1210 4431 BclI T'GATC_A Cuts at: 1548 1548 Size: 5641 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2851 4252 136 Size: 107 122 71 631 605 1179 1401 1525 Fragments arranged by size: 1525 1401 1179 631 605 122 107 71 BglII A'GATC_T Cuts at: 5636 5636 Size: 5641 BlpI GC'TnA_GC Cuts at: 2150 2477 2886 3058 2150 Size: 327 409 172 4733 Fragments arranged by size: 4733 409 327 172 Bme1580I G_kGCm'C Cuts at: 1790 2452 2787 2830 3702 4858 1790 Size: 662 335 43 872 1156 2573 Fragments arranged by size: 2573 1156 872 662 335 43 BmgBI CAC'GTC Cuts at: 1318 2197 1318 Size: 879 4762 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2488 3372 4330 Size: 416 53 83 399 24 1071 884 958 Cuts at: 4330 442 Size: 1753 Fragments arranged by size: 1753 1071 958 884 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5641 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2846 4301 1239 Size: 1607 1455 2579 Fragments arranged by size: 2579 1607 1455 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3766 4631 4929 3766 Size: 865 298 4478 Fragments arranged by size: 4478 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2900 4304 882 Size: 617 1401 1404 2219 Fragments arranged by size: 2219 1404 1401 617 BsaAI yAC'GTr Cuts at: 493 1204 2035 3432 493 Size: 711 831 1397 2702 Fragments arranged by size: 2702 1397 831 711 BsaBI GATnn'nnATC Cuts at: 5635 5635 Size: 5641 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2052 2484 3448 3830 Size: 53 83 186 936 519 432 964 382 Cuts at: 3830 4465 275 Size: 635 1451 Fragments arranged by size: 1451 964 936 635 519 432 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4074 4472 4746 4893 5304 4074 Size: 398 274 147 411 4411 Fragments arranged by size: 4411 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5416 Fragments arranged by size: 5416 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2654 2666 1186 Size: 1468 12 4161 Fragments arranged by size: 4161 1468 12 BseYI C'CCAG_C Cuts at: 2122 2386 2510 2625 2872 2890 4844 5340 2122 Size: 264 124 115 247 18 1954 496 2423 Fragments arranged by size: 2423 1954 496 264 247 124 115 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2309 2450 2473 1163 Size: 573 573 141 23 4331 Fragments arranged by size: 4331 573 573 141 23 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2352 3091 3852 4880 1540 Size: 275 186 351 739 761 1028 2301 Fragments arranged by size: 2301 1028 761 739 351 275 186 BsiHKAI G_wGCw'C Cuts at: 2787 3702 3787 4858 2787 Size: 915 85 1071 3570 Fragments arranged by size: 3570 1071 915 85 BsmI GAATG_Cn' Cuts at: 3152 3245 3152 Size: 93 5548 BsmBI CGTCTCn'nnnn_ Cuts at: 2871 2871 Size: 5641 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2106 2452 2511 2658 2787 2830 Size: 399 226 90 346 59 147 129 43 Cuts at: 2830 2960 3702 3787 4858 1391 Size: 130 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 346 226 147 130 129 90 85 59 43 BspEI T'CCGG_A Cuts at: 5304 5304 Size: 5641 BspHI T'CATG_A Cuts at: 3530 5260 3530 Size: 1730 3911 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2478 2736 2996 844 Size: 1634 258 260 3489 Fragments arranged by size: 3489 1634 260 258 BsrBI CCG'CTC Cuts at: 1394 1823 3528 1394 Size: 429 1705 3507 Fragments arranged by size: 3507 1705 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4136 4310 65 Size: 1183 2888 174 1396 Fragments arranged by size: 2888 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2265 4285 4472 1700 Size: 110 455 2020 187 2869 Fragments arranged by size: 2869 2020 455 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5641 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5641 BssSI C'ACGA_G Cuts at: 2398 3701 4713 2398 Size: 1303 1012 3326 Fragments arranged by size: 3326 1303 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2473 794 Size: 1679 3962 BstBI TT'CG_AA Cuts at: 4445 4445 Size: 5641 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5641 BstYI r'GATC_y Cuts at: 1972 2209 2971 3338 3723 3740 5192 5278 5290 Size: 237 762 367 385 17 1452 86 12 Cuts at: 5290 5636 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 762 385 367 346 237 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5089 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4902 Fragments arranged by size: 4902 683 56 BtsI GCAGTG_nn' Cuts at: 2372 2785 2830 3157 3951 3971 2372 Size: 413 45 327 794 20 4042 Fragments arranged by size: 4042 794 413 327 45 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5641 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5606 DraI TTT'AAA Cuts at: 2945 2945 Size: 5641 DraIII CAC_nnn'GTG Cuts at: 2236 2580 2236 Size: 344 5297 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4648 809 Size: 507 3332 1802 Fragments arranged by size: 3332 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2238 2349 2373 Size: 54 1425 50 275 18 408 111 24 Cuts at: 2373 2775 2793 3088 3977 5606 8 Size: 402 18 295 889 1629 43 Fragments arranged by size: 1629 1425 889 408 402 295 275 111 54 50 43 24 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2349 3088 1537 Size: 275 537 739 4090 Fragments arranged by size: 4090 739 537 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2387 2684 2690 3571 1353 Size: 1034 297 6 881 3423 Fragments arranged by size: 3423 1034 881 297 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4228 4602 4748 387 Size: 1064 138 2639 374 146 1280 Fragments arranged by size: 2639 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2746 2846 3704 4301 5088 5356 1239 Size: 393 1114 100 858 597 787 268 1524 Fragments arranged by size: 1524 1114 858 787 597 393 268 100 EcoNI CCTnn'n_nnAGG Cuts at: 2096 2096 Size: 5641 EcoO109I rG'GnC_Cy Cuts at: 2449 2621 2920 2449 Size: 172 299 5170 Fragments arranged by size: 5170 299 172 EcoRI G'AATT_C Cuts at: 2950 3328 2950 Size: 378 5263 FspI TGC'GCA Cuts at: 4147 4147 Size: 5641 HaeII r_GCGC'y Cuts at: 1678 2055 2487 2873 4788 1678 Size: 377 432 386 1915 2531 Fragments arranged by size: 2531 1915 432 386 377 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373 Size: 32 135 32 2710 32 42 32 2626 Fragments arranged by size: 2710 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2985 3231 669 Size: 988 281 1047 246 3079 Fragments arranged by size: 3079 1047 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5641 HpaI GTT'AAC Cuts at: 3231 3231 Size: 5641 KasI G'GCGC_C Cuts at: 2051 2483 2051 Size: 432 5209 KpnI G_GTAC'C Cuts at: 2966 2966 Size: 5641 MfeI C'AATT_G Cuts at: 3240 3240 Size: 5641 MluI A'CGCG_T Cuts at: 5517 5517 Size: 5641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4755 4939 1305 Size: 3450 184 2007 Fragments arranged by size: 3450 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2240 10 Size: 54 1425 343 408 3411 Fragments arranged by size: 3411 1425 408 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2429 3599 3958 4117 518 Size: 648 1263 1170 359 159 2042 Fragments arranged by size: 2042 1263 1170 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2481 2549 2629 3057 3734 4882 5127 1606 Size: 875 68 80 428 677 1148 245 2120 Fragments arranged by size: 2120 1148 875 677 428 245 80 68 NaeI GCC'GGC Cuts at: 2267 2267 Size: 5641 NarI GG'CG_CC Cuts at: 2052 2484 2052 Size: 432 5209 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5089 NdeI CA'TA_TG Cuts at: 387 387 Size: 5641 NgoMIV G'CCGG_C Cuts at: 2265 2265 Size: 5641 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5641 NotI GC'GGCC_GC Cuts at: 3088 3088 Size: 5641 NspI r_CATG'y Cuts at: 1132 1229 2999 4544 1132 Size: 97 1770 1545 2229 Fragments arranged by size: 2229 1770 1545 97 PasI CC'CwG_GG Cuts at: 1348 2651 2830 1348 Size: 1303 179 4159 Fragments arranged by size: 4159 1303 179 PciI A'CATG_T Cuts at: 4540 4540 Size: 5641 PflMI CCAn_nnn'nTGG Cuts at: 1108 2236 1108 Size: 1128 4513 PfoI T'CCnGG_A Cuts at: 2190 2720 2190 Size: 530 5111 PmeI GTTT'AAAC Cuts at: 2945 2945 Size: 5641 PmlI CAC'GTG Cuts at: 2035 2035 Size: 5641 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 5223 5255 3710 Size: 32 1481 32 4096 Fragments arranged by size: 4096 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5641 PsiI TTA'TAA Cuts at: 3211 3211 Size: 5641 PspOMI G'GGCC_C Cuts at: 2448 2448 Size: 5641 PstI C_TGCA'G Cuts at: 830 2993 830 Size: 2163 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5641 SalI G'TCGA_C Cuts at: 1936 2983 1936 Size: 1047 4594 SbfI CC_TGCA'GG Cuts at: 2993 2993 Size: 5641 ScaI AGT'ACT Cuts at: 3889 5601 3889 Size: 1712 3929 SfcI C'TryA_G Cuts at: 826 1045 2164 2989 4124 4805 4996 826 Size: 219 1119 825 1135 681 191 1471 Fragments arranged by size: 1471 1135 1119 825 681 219 191 SfoI GGC'GCC Cuts at: 2053 2485 2053 Size: 432 5209 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5641 SmaI CCC'GGG Cuts at: 1872 2280 2642 2968 1872 Size: 408 362 326 4545 Fragments arranged by size: 4545 408 362 326 SmlI C'TyrA_G Cuts at: 820 1017 1943 3745 4646 4908 820 Size: 197 926 1802 901 262 1553 Fragments arranged by size: 1802 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3432 493 Size: 2939 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5641 SphI G_CATG'C Cuts at: 1132 2999 1132 Size: 1867 3774 SspI AAT'ATT Cuts at: 5 52 3565 5 Size: 47 3513 2081 Fragments arranged by size: 3513 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5641 StyI C'CwwG_G Cuts at: 513 1065 2221 3080 513 Size: 552 1156 859 3074 Fragments arranged by size: 3074 1156 859 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2507 3668 3685 3838 4023 4468 325 Size: 1255 927 1161 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1161 927 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3887 5599 96 Size: 274 80 33 51 1443 1910 1712 138 Fragments arranged by size: 1910 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2019 2051 5601 5633 2019 Size: 32 3550 32 2027 Fragments arranged by size: 3550 2027 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5641 XbaI T'CTAG_A Cuts at: 2977 2977 Size: 5641 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2188 2188 Size: 5641 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5641 XmaI C'CCGG_G Cuts at: 1870 2278 2640 2966 1870 Size: 408 362 326 4545 Fragments arranged by size: 4545 408 362 326 XmnI GAAnn'nnTTC Cuts at: 3770 3770 Size: 5641 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3449 276 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI