Restriction map for
pFN21AE0803
(Linear) (Six-base) MAPSORT of: KIEE0803.seq Check: 1452 from: 1 to: 936
KIEE0803.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 473 936
Size: 473 463
AccI GT'mk_AC
Cuts at: 0 158 803 936
Size: 158 645 133
Fragments arranged by size:
645 158 133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 741 936
Size: 741 195
AfeI AGC'GCT
Cuts at: 0 866 936
Size: 866 70
AleI CACnn'nnGTG
Cuts at: 0 424 936
Size: 424 512
AlwNI CAG_nnn'CTG
Cuts at: 0 366 936
Size: 366 570
ApaI G_GGCC'C
Cuts at: 0 447 936
Size: 447 489
ApaLI G'TGCA_C
Cuts at: 0 778 936
Size: 778 158
AvaI C'yCGr_G
Cuts at: 0 273 635 936
Size: 273 362 301
Fragments arranged by size:
362 301 273
BanI G'GyrC_C
Cuts at: 0 46 478 936
Size: 46 432 458
Fragments arranged by size:
458 432 46
BanII G_rGCy'C
Cuts at: 0 11 101 447 506 653 936
Size: 11 90 346 59 147 283
Fragments arranged by size:
346 283 147 90 59 11
BbeI G_GCGC'C
Cuts at: 0 50 482 936
Size: 50 432 454
Fragments arranged by size:
454 432 50
BglI GCCn_nnn'nGGC
Cuts at: 0 846 936
Size: 846 90
BlpI GC'TnA_GC
Cuts at: 0 145 472 881 936
Size: 145 327 409 55
Fragments arranged by size:
409 327 145 55
Bme1580I G_kGCm'C
Cuts at: 0 447 782 825 936
Size: 447 335 43 111
Fragments arranged by size:
447 335 111 43
BmgBI CAC'GTC
Cuts at: 0 192 936
Size: 192 744
BmrI ACTGGGnnnn_n'
Cuts at: 0 483 936
Size: 483 453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 841 936
Size: 841 95
BsaI GGTCTCn'nnnn_
Cuts at: 0 895 936
Size: 895 41
BsaAI yAC'GTr
Cuts at: 0 30 936
Size: 30 906
BsaHI Gr'CG_yC
Cuts at: 0 47 479 936
Size: 47 432 457
Fragments arranged by size:
457 432 47
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 649 661 936
Size: 649 12 275
Fragments arranged by size:
649 275 12
BseYI C'CCAG_C
Cuts at: 0 117 381 505 620 867 885 936
Size: 117 264 124 115 247 18 51
Fragments arranged by size:
264 247 124 117 115 51 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 445 468 936
Size: 304 141 23 468
Fragments arranged by size:
468 304 141 23
BsiEI CG_ry'CG
Cuts at: 0 347 936
Size: 347 589
BsiHKAI G_wGCw'C
Cuts at: 0 782 936
Size: 782 154
BsmBI CGTCTCn'nnnn_
Cuts at: 0 866 936
Size: 866 70
Bsp1286I G_dGCh'C
Cuts at: 0 11 101 447 506 653 782 825 936
Size: 11 90 346 59 147 129 43 111
Fragments arranged by size:
346 147 129 111 90 59 43 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 473 731 936
Size: 473 258 205
Fragments arranged by size:
473 258 205
BsrFI r'CCGG_y
Cuts at: 0 260 936
Size: 260 676
BssSI C'ACGA_G
Cuts at: 0 393 936
Size: 393 543
BstAPI GCAn_nnn'nTGC
Cuts at: 0 468 936
Size: 468 468
BstYI r'GATC_y
Cuts at: 0 204 936
Size: 204 732
BtsI GCAGTG_nn'
Cuts at: 0 367 780 825 936
Size: 367 413 45 111
Fragments arranged by size:
413 367 111 45
DraIII CAC_nnn'GTG
Cuts at: 0 231 575 936
Size: 231 344 361
Fragments arranged by size:
361 344 231
EaeI y'GGCC_r
Cuts at: 0 233 344 368 770 788 936
Size: 233 111 24 402 18 148
Fragments arranged by size:
402 233 148 111 24 18
EagI C'GGCC_G
Cuts at: 0 344 936
Size: 344 592
EarI CTCTTCn'nnn_
Cuts at: 0 382 679 685 936
Size: 382 297 6 251
Fragments arranged by size:
382 297 251 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 741 841 936
Size: 741 100 95
Fragments arranged by size:
741 100 95
EcoNI CCTnn'n_nnAGG
Cuts at: 0 91 936
Size: 91 845
EcoO109I rG'GnC_Cy
Cuts at: 0 444 616 915 936
Size: 444 172 299 21
Fragments arranged by size:
444 299 172 21
HaeII r_GCGC'y
Cuts at: 0 50 482 868 936
Size: 50 432 386 68
Fragments arranged by size:
432 386 68 50
KasI G'GCGC_C
Cuts at: 0 46 478 936
Size: 46 432 458
Fragments arranged by size:
458 432 46
MscI TGG'CCA
Cuts at: 0 235 936
Size: 235 701
MslI CAynn'nnrTG
Cuts at: 0 424 936
Size: 424 512
MspA1I CmG'CkG
Cuts at: 0 476 544 624 936
Size: 476 68 80 312
Fragments arranged by size:
476 312 80 68
NaeI GCC'GGC
Cuts at: 0 262 936
Size: 262 674
NarI GG'CG_CC
Cuts at: 0 47 479 936
Size: 47 432 457
Fragments arranged by size:
457 432 47
NgoMIV G'CCGG_C
Cuts at: 0 260 936
Size: 260 676
PasI CC'CwG_GG
Cuts at: 0 646 825 936
Size: 646 179 111
Fragments arranged by size:
646 179 111
PflMI CCAn_nnn'nTGG
Cuts at: 0 231 936
Size: 231 705
PfoI T'CCnGG_A
Cuts at: 0 185 715 936
Size: 185 530 221
Fragments arranged by size:
530 221 185
PmlI CAC'GTG
Cuts at: 0 30 936
Size: 30 906
PspOMI G'GGCC_C
Cuts at: 0 443 936
Size: 443 493
SfcI C'TryA_G
Cuts at: 0 159 936
Size: 159 777
SfoI GGC'GCC
Cuts at: 0 48 480 936
Size: 48 432 456
Fragments arranged by size:
456 432 48
SmaI CCC'GGG
Cuts at: 0 275 637 936
Size: 275 362 299
Fragments arranged by size:
362 299 275
StyI C'CwwG_G
Cuts at: 0 216 936
Size: 216 720
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 502 936
Size: 502 434
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 14 46 936
Size: 14 32 890
Fragments arranged by size:
890 32 14
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 183 936
Size: 183 753
XmaI C'CCGG_G
Cuts at: 0 273 635 936
Size: 273 362 301
Fragments arranged by size:
362 301 273
Enzymes that do cut:
AarI AccI AcuI AfeI AleI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BglI BlpI Bme1580I BmgBI
BmrI BpmI BsaI BsaAI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssSI BstAPI
BstYI BtsI DraIII EaeI EagI EarI Eco57MI EcoNI
EcoO109I HaeII KasI MscI MslI MspA1I NaeI NarI
NgoMIV PasI PflMI PfoI PmlI PspOMI SfcI SfoI
SmaI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmtI
BplI Bpu10I BpuEI BsaBI BsaWI BsaXI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DrdI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PciI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI XmnI ZraI