DOK2 ORF clone pFN21AE0716

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Halotag ORF Clone pFN21AE0716

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Clone info.

Species

human

Clone Name

pFN21AE0716 Sequence (with vector) Restriction Map

Product ID

FHC11250

Flexi Clone PID

FXC11250

ORF Sequence

DNA sequence (1236 bp)
Protein sequence (412 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528923]

Gene

KIEE0716

Symbol

DOK2

Alias : p56DOK, p56dok-2

Description

docking protein 2, 56kDa

Original Clone ID

cs02216

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1236 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 412 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS6016.1 1.1e-92 100.0 DOK2 CCDS78098.1 5.3e-18 39.0 DOK3 CCDS4426.1 5.8e-18 39.0 DOK3 CCDS1954.1 5.5e-15 39.1 DOK1 CCDS47350.1 1.6e-11 46.1 DOK3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR002404 149 246 PF02174 Insulin receptor substrate-1 HMMSmart IPR001849 5 116 SM00233 Pleckstrin homology domain IPR002404 148 247 SM00310 Insulin receptor substrate-1 ProfileScan IPR001849 4 114 PS50003 Pleckstrin homology domain IPR002404 147 252 PS51064 Insulin receptor substrate-1
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_003965 412 604997 100.0 100.0 XP_011532958 440 611435 39.0 97.3 NP_001295164 440 611435 39.0 97.3 XP_011532957 440 611435 39.0 97.3 XP_005266042 440 611435 39.0 97.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0716 Download

>KIEE0716  1236 bp
ATGGGAGACGGGGCAGTGAAACAAGGCTTCTTGTATCTTCAGCAGCAGCAGACGTTTGGA
AAGAAATGGCGCCGCTTCGGCGCCTCACTGTATGGAGGGTCGGACTGCGCCTTGGCCCGG
CTGGAGCTGCAGGAGGGCCCGGAGAAGCCTCGTCGGTGTGAGGCTGCCCGGAAGGTCATC
CGCCTCAGTGACTGCCTGCGGGTGGCCGAGGCCGGCGGAGAGGCCAGCAGCCCCCGGGAC
ACCAGTGCCTTCTTCCTGGAGACCAAGGAGCGCCTGTACCTCCTGGCGGCCCCTGCAGCG
GAGCGCGGCGACTGGGTGCAGGCCATCTGCCTCCTGGCCTTCCCCGGGCAGAGGAAGGAG
CTCTCGGGGCCAGAGGGAAAGCAGAGCCGGCCCTGCATGGAGGAAAATGAATTGTACAGC
AGCGCAGTCACAGTCGGCCCCCACAAGGAATTTGCTGTGACCATGAGACCTACAGAAGCC
AGTGAGAGGTGCCACCTGCGGGGGTCCTATACCCTCCGGGCTGGGGAGAGTGCCCTGGAG
CTGTGGGGTGGGCCCGAGCCAGGGACCCAGCTGTACGACTGGCCCTACAGGTTTCTGCGG
CGCTTTGGGCGGGACAAGGTAACCTTTTCCTTTGAGGCAGGCCGTCGCTGCGTCTCTGGA
GAGGGCAACTTTGAGTTCGAAACCCGGCAAGGCAATGAGATCTTCTTGGCCCTGGAAGAG
GCCATCTCTGCCCAGAAGAATGCTGCACCCGCTACACCCCAACCGCAGCCAGCCACAATC
CCCGCGTCGCTGCCCCGGCCTGATAGCCCCTACTCTCGGCCGCATGACTCACTGCCGCCG
CCTTCACCCACCACACCGGTGCCTGCTCCACGGCCTCGGGGCCAGGAGGGGGAGTATGCC
GTGCCCTTCGATGCGGTGGCCCGTTCCTTGGGGAAGAACTTCAGGGGCATCTTGGCAGTC
CCTCCTCAGCTCCTGGCCGACCCTCTGTACGACAGCATTGAGGAGACCCTGCCCCCTCGA
CCTGACCACATATACGATGAGCCCGAGGGAGTGGCTGCCCTGTCCCTCTATGACAGCCCG
CAGGAGCCCCGGGGTGAGGCATGGAGGAGGCAGGCGACAGCTGACAGGGACCCTGCTGGC
CTCCAGCATGTCCAGCCAGCCGGGCAGGATTTCTCTGCTTCTGGCTGGCAGCCAGGAACT
GAGTATGACAATGTTGTACTAAAGAAAGGCCCAAAG

ORF Protein

Cloned ORF protein sequence for pFN21AE0716 Download

>KIEE0716  412 aa
MGDGAVKQGFLYLQQQQTFGKKWRRFGASLYGGSDCALARLELQEGPEKPRRCEAARKVI
RLSDCLRVAEAGGEASSPRDTSAFFLETKERLYLLAAPAAERGDWVQAICLLAFPGQRKE
LSGPEGKQSRPCMEENELYSSAVTVGPHKEFAVTMRPTEASERCHLRGSYTLRAGESALE
LWGGPEPGTQLYDWPYRFLRRFGRDKVTFSFEAGRRCVSGEGNFEFETRQGNEIFLALEE
AISAQKNAAPATPQPQPATIPASLPRPDSPYSRPHDSLPPPSPTTPVPAPRPRGQEGEYA
VPFDAVARSLGKNFRGILAVPPQLLADPLYDSIEETLPPRPDHIYDEPEGVAALSLYDSP
QEPRGEAWRRQATADRDPAGLQHVQPAGQDFSASGWQPGTEYDNVVLKKGPK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0716 Download

>pFN21AE0716  5941 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGAGACGGGGCAGTGAAACAAGGCTTCTTGTA
TCTTCAGCAGCAGCAGACGTTTGGAAAGAAATGGCGCCGCTTCGGCGCCTCACTGTATGG
AGGGTCGGACTGCGCCTTGGCCCGGCTGGAGCTGCAGGAGGGCCCGGAGAAGCCTCGTCG
GTGTGAGGCTGCCCGGAAGGTCATCCGCCTCAGTGACTGCCTGCGGGTGGCCGAGGCCGG
CGGAGAGGCCAGCAGCCCCCGGGACACCAGTGCCTTCTTCCTGGAGACCAAGGAGCGCCT
GTACCTCCTGGCGGCCCCTGCAGCGGAGCGCGGCGACTGGGTGCAGGCCATCTGCCTCCT
GGCCTTCCCCGGGCAGAGGAAGGAGCTCTCGGGGCCAGAGGGAAAGCAGAGCCGGCCCTG
CATGGAGGAAAATGAATTGTACAGCAGCGCAGTCACAGTCGGCCCCCACAAGGAATTTGC
TGTGACCATGAGACCTACAGAAGCCAGTGAGAGGTGCCACCTGCGGGGGTCCTATACCCT
CCGGGCTGGGGAGAGTGCCCTGGAGCTGTGGGGTGGGCCCGAGCCAGGGACCCAGCTGTA
CGACTGGCCCTACAGGTTTCTGCGGCGCTTTGGGCGGGACAAGGTAACCTTTTCCTTTGA
GGCAGGCCGTCGCTGCGTCTCTGGAGAGGGCAACTTTGAGTTCGAAACCCGGCAAGGCAA
TGAGATCTTCTTGGCCCTGGAAGAGGCCATCTCTGCCCAGAAGAATGCTGCACCCGCTAC
ACCCCAACCGCAGCCAGCCACAATCCCCGCGTCGCTGCCCCGGCCTGATAGCCCCTACTC
TCGGCCGCATGACTCACTGCCGCCGCCTTCACCCACCACACCGGTGCCTGCTCCACGGCC
TCGGGGCCAGGAGGGGGAGTATGCCGTGCCCTTCGATGCGGTGGCCCGTTCCTTGGGGAA
GAACTTCAGGGGCATCTTGGCAGTCCCTCCTCAGCTCCTGGCCGACCCTCTGTACGACAG
CATTGAGGAGACCCTGCCCCCTCGACCTGACCACATATACGATGAGCCCGAGGGAGTGGC
TGCCCTGTCCCTCTATGACAGCCCGCAGGAGCCCCGGGGTGAGGCATGGAGGAGGCAGGC
GACAGCTGACAGGGACCCTGCTGGCCTCCAGCATGTCCAGCCAGCCGGGCAGGATTTCTC
TGCTTCTGGCTGGCAGCCAGGAACTGAGTATGACAATGTTGTACTAAAGAAAGGCCCAAA
GGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
T