Restriction map for
pFN21AE0716
(Linear) (Six-base) MAPSORT of: KIEE0716.seq Check: 6486 from: 1 to: 1236
KIEE0716.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 503 1236
Size: 503 733
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 924 1236
Size: 22 902 312
Fragments arranged by size:
902 312 22
AgeI A'CCGG_T
Cuts at: 0 855 1236
Size: 855 381
AleI CACnn'nnGTG
Cuts at: 0 856 1236
Size: 856 380
AlwNI CAG_nnn'CTG
Cuts at: 0 191 972 1198 1236
Size: 191 781 226 38
Fragments arranged by size:
781 226 191 38
ApaI G_GGCC'C
Cuts at: 0 139 554 1236
Size: 139 415 682
Fragments arranged by size:
682 415 139
ApoI r'AATT_y
Cuts at: 0 448 1236
Size: 448 788
AvaI C'yCGr_G
Cuts at: 0 233 343 363 553 875 1042 1088 1236
Size: 233 110 20 190 322 167 46 148
Fragments arranged by size:
322 233 190 167 148 110 46 20
BanI G'GyrC_C
Cuts at: 0 68 79 488 858 1236
Size: 68 11 409 370 378
Fragments arranged by size:
409 378 370 68 11
BanII G_rGCy'C
Cuts at: 0 139 362 554 1043 1088 1236
Size: 139 223 192 489 45 148
Fragments arranged by size:
489 223 192 148 139 45
BbeI G_GCGC'C
Cuts at: 0 72 83 1236
Size: 72 11 1153
Fragments arranged by size:
1153 72 11
BbvCI CC'TCA_GC
Cuts at: 0 965 1236
Size: 965 271
BglI GCCn_nnn'nGGC
Cuts at: 0 77 1161 1236
Size: 77 1084 75
Fragments arranged by size:
1084 77 75
BglII A'GATC_T
Cuts at: 0 698 1236
Size: 698 538
Bme1580I G_kGCm'C
Cuts at: 0 139 534 554 905 1236
Size: 139 395 20 351 331
Fragments arranged by size:
395 351 331 139 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 321 1236
Size: 321 915
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 741 1236
Size: 741 495
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 277 556 677 1126 1236
Size: 142 135 279 121 449 110
Fragments arranged by size:
449 279 142 135 121 110
Bpu10I CC'TnA_GC
Cuts at: 0 965 1236
Size: 965 271
BsaI GGTCTCn'nnnn_
Cuts at: 0 253 459 997 1236
Size: 253 206 538 239
Fragments arranged by size:
538 253 239 206
BsaHI Gr'CG_yC
Cuts at: 0 69 80 1236
Size: 69 11 1156
Fragments arranged by size:
1156 69 11
BsaWI w'CCGG_w
Cuts at: 0 855 1236
Size: 855 381
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 259 289 877 907 1236
Size: 259 30 588 30 329
Fragments arranged by size:
588 329 259 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 953 1015 1119 1236
Size: 953 62 104 117
Fragments arranged by size:
953 117 104 62
BseYI C'CCAG_C
Cuts at: 0 520 566 1236
Size: 520 46 670
Fragments arranged by size:
670 520 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 728 1236
Size: 337 391 508
Fragments arranged by size:
508 391 337
BsiEI CG_ry'CG
Cuts at: 0 820 1236
Size: 820 416
BsiHKAI G_wGCw'C
Cuts at: 0 362 1236
Size: 362 874
BsmI GAATG_Cn'
Cuts at: 0 744 1236
Size: 744 492
BsmBI CGTCTCn'nnnn_
Cuts at: 0 657 1236
Size: 657 579
Bsp1286I G_dGCh'C
Cuts at: 0 139 362 534 554 905 1043 1088 1236
Size: 139 223 172 20 351 138 45 148
Fragments arranged by size:
351 223 172 148 139 138 45 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 503 1236
Size: 503 733
BsrDI GCAATG_nn'
Cuts at: 0 699 1236
Size: 699 537
BsrFI r'CCGG_y
Cuts at: 0 211 386 855 1236
Size: 211 175 469 381
Fragments arranged by size:
469 381 211 175
BsrGI T'GTAC_A
Cuts at: 0 413 1236
Size: 413 823
BstBI TT'CG_AA
Cuts at: 0 677 1236
Size: 677 559
BstEII G'GTnAC_C
Cuts at: 0 618 1236
Size: 618 618
BstYI r'GATC_y
Cuts at: 0 698 1236
Size: 698 538
BtgI C'CryG_G
Cuts at: 0 868 1236
Size: 868 368
BtsI GCAGTG_nn'
Cuts at: 0 20 829 1236
Size: 20 809 407
Fragments arranged by size:
809 407 20
EaeI y'GGCC_r
Cuts at: 0 203 817 974 1236
Size: 203 614 157 262
Fragments arranged by size:
614 262 203 157
EagI C'GGCC_G
Cuts at: 0 817 1236
Size: 817 419
EarI CTCTTCn'nnn_
Cuts at: 0 710 1236
Size: 710 526
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 169 230 1236
Size: 169 61 1006
Fragments arranged by size:
1006 169 61
EcoICRI GAG'CTC
Cuts at: 0 360 1236
Size: 360 876
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 142 277 556 677 924 1126 1236
Size: 22 120 135 279 121 247 202 110
Fragments arranged by size:
279 247 202 135 121 120 110 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 715 1236
Size: 715 521
EcoO109I rG'GnC_Cy
Cuts at: 0 135 503 563 1128 1236
Size: 135 368 60 565 108
Fragments arranged by size:
565 368 135 108 60
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 639 1236
Size: 639 597
HaeII r_GCGC'y
Cuts at: 0 72 83 273 603 1236
Size: 72 11 190 330 633
Fragments arranged by size:
633 330 190 72 11
KasI G'GCGC_C
Cuts at: 0 68 79 1236
Size: 68 11 1157
Fragments arranged by size:
1157 68 11
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 1236
Size: 80 1156
MslI CAynn'nnrTG
Cuts at: 0 856 1236
Size: 856 380
MspA1I CmG'CkG
Cuts at: 0 298 570 1120 1236
Size: 298 272 550 116
Fragments arranged by size:
550 298 272 116
NaeI GCC'GGC
Cuts at: 0 213 388 1236
Size: 213 175 848
Fragments arranged by size:
848 213 175
NarI GG'CG_CC
Cuts at: 0 69 80 1236
Size: 69 11 1156
Fragments arranged by size:
1156 69 11
NgoMIV G'CCGG_C
Cuts at: 0 211 386 1236
Size: 211 175 850
Fragments arranged by size:
850 211 175
NspI r_CATG'y
Cuts at: 0 1150 1236
Size: 1150 86
PfoI T'CCnGG_A
Cuts at: 0 254 1236
Size: 254 982
PpuMI rG'GwC_Cy
Cuts at: 0 503 563 1128 1236
Size: 503 60 565 108
Fragments arranged by size:
565 503 108 60
PspOMI G'GGCC_C
Cuts at: 0 135 550 1236
Size: 135 415 686
Fragments arranged by size:
686 415 135
PstI C_TGCA'G
Cuts at: 0 131 297 1236
Size: 131 166 939
Fragments arranged by size:
939 166 131
PvuII CAG'CTG
Cuts at: 0 570 1120 1236
Size: 570 550 116
Fragments arranged by size:
570 550 116
SacI G_AGCT'C
Cuts at: 0 362 1236
Size: 362 874
SanDI GG'GwC_CC
Cuts at: 0 563 1128 1236
Size: 563 565 108
Fragments arranged by size:
565 563 108
SfcI C'TryA_G
Cuts at: 0 127 293 470 585 1236
Size: 127 166 177 115 651
Fragments arranged by size:
651 177 166 127 115
SfoI GGC'GCC
Cuts at: 0 70 81 1236
Size: 70 11 1155
Fragments arranged by size:
1155 70 11
SgrAI Cr'CCGG_yG
Cuts at: 0 855 1236
Size: 855 381
SmaI CCC'GGG
Cuts at: 0 235 345 1090 1236
Size: 235 110 745 146
Fragments arranged by size:
745 235 146 110
StyI C'CwwG_G
Cuts at: 0 110 263 926 1236
Size: 110 153 663 310
Fragments arranged by size:
663 310 153 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 303 861 1236
Size: 303 558 375
Fragments arranged by size:
558 375 303
TatI w'GTAC_w
Cuts at: 0 413 1215 1236
Size: 413 802 21
Fragments arranged by size:
802 413 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 330 1236
Size: 330 906
XmaI C'CCGG_G
Cuts at: 0 233 343 1088 1236
Size: 233 110 745 148
Fragments arranged by size:
745 233 148 110
XmnI GAAnn'nnTTC
Cuts at: 0 937 1236
Size: 937 299
Enzymes that do cut:
AarI AcuI AgeI AleI AlwNI ApaI ApoI AvaI
BanI BanII BbeI BbvCI BglI BglII Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrDI BsrFI BsrGI BstBI BstEII BstYI BtgI BtsI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII KasI MmeI MslI MspA1I NaeI NarI
NgoMIV NspI PfoI PpuMI PspOMI PstI PvuII SacI
SanDI SfcI SfoI SgrAI SmaI StyI TaqII TatI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BcgI BciVI BclI BfrBI BlpI BmgBI
BmtI BpuEI BsaAI BsaBI BsiWI BspEI BspHI BsrBI
BssHII BssSI BstAPI BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EcoRI EcoRV FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MscI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SgfI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI
ZraI