(Circular) (Six-base) MAPSORT of: pFN21AE0716.seq Check: 1998 from: 1 to: 5941 pFN21AE0716 5941 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2508 2508 Size: 5941 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3751 278 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3284 1937 Size: 1347 4594 Acc65I G'GTAC_C Cuts at: 3262 3262 Size: 5941 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2027 2929 4004 5388 5656 1632 Size: 395 902 1075 1384 268 1917 Fragments arranged by size: 1917 1384 1075 902 395 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 4840 5817 1203 Size: 3637 977 1327 Fragments arranged by size: 3637 1327 977 AgeI A'CCGG_T Cuts at: 2860 4772 2860 Size: 1912 4029 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 2861 1166 Size: 1695 4246 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5941 AlwNI CAG_nnn'CTG Cuts at: 2196 2977 3203 3684 5256 2196 Size: 781 226 481 1572 2881 Fragments arranged by size: 2881 1572 781 481 226 ApaI G_GGCC'C Cuts at: 2144 2559 2144 Size: 415 5526 ApaLI G'TGCA_C Cuts at: 3998 5154 3998 Size: 1156 4785 ApoI r'AATT_y Cuts at: 1493 2453 3250 3476 3628 5737 1493 Size: 960 797 226 152 2109 1697 Fragments arranged by size: 2109 1697 960 797 226 152 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2238 2348 2368 2558 2880 3047 3093 Size: 73 295 110 20 190 322 167 46 Cuts at: 3093 3266 1870 Size: 173 4545 Fragments arranged by size: 4545 322 295 190 173 167 110 73 46 20 BamHI G'GATC_C Cuts at: 3271 3638 3271 Size: 367 5574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2073 2084 2493 2863 3262 Size: 325 217 627 286 11 409 370 399 Cuts at: 3262 4717 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 409 399 370 325 286 217 11 BanII G_rGCy'C Cuts at: 1391 2144 2367 2559 3048 3093 3260 1391 Size: 753 223 192 489 45 167 4072 Fragments arranged by size: 4072 753 489 223 192 167 45 BbeI G_GCGC'C Cuts at: 2077 2088 2077 Size: 11 5930 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4970 BbvCI CC'TCA_GC Cuts at: 2970 2970 Size: 5941 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4132 4166 1903 Size: 34 2195 34 3678 Fragments arranged by size: 3678 2195 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3833 5043 3833 Size: 1210 4731 BclI T'GATC_A Cuts at: 1548 1548 Size: 5941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2082 3166 4552 Size: 107 122 71 631 605 410 1084 1386 Cuts at: 4552 136 Size: 1525 Fragments arranged by size: 1525 1386 1084 631 605 410 122 107 71 BglII A'GATC_T Cuts at: 2703 5936 2703 Size: 3233 2708 BlpI GC'TnA_GC Cuts at: 3358 3358 Size: 5941 Bme1580I G_kGCm'C Cuts at: 1790 2144 2539 2559 2910 4002 5158 1790 Size: 354 395 20 351 1092 1156 2573 Fragments arranged by size: 2573 1156 1092 395 354 351 20 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5941 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2326 3672 4630 Size: 416 53 83 399 24 909 1346 958 Cuts at: 4630 442 Size: 1753 Fragments arranged by size: 1753 1346 958 909 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2746 2746 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2147 2282 2561 2682 3131 4601 1239 Size: 908 135 279 121 449 1470 2579 Fragments arranged by size: 2579 1470 908 449 279 135 121 Bpu10I CC'TnA_GC Cuts at: 2970 2970 Size: 5941 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4066 4931 5229 4066 Size: 865 298 4778 Fragments arranged by size: 4778 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2258 2464 3002 4604 882 Size: 617 759 206 538 1602 2219 Fragments arranged by size: 2219 1602 759 617 538 206 BsaAI yAC'GTr Cuts at: 493 1204 3732 493 Size: 711 2528 2702 Fragments arranged by size: 2702 2528 711 BsaBI GATnn'nnATC Cuts at: 5935 5935 Size: 5941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2074 2085 3748 4130 Size: 53 83 186 936 541 11 1663 382 Cuts at: 4130 4765 275 Size: 635 1451 Fragments arranged by size: 1663 1451 936 635 541 382 186 83 53 11 BsaWI w'CCGG_w Cuts at: 2860 4374 4772 5046 5193 5604 2860 Size: 1514 398 274 147 411 3197 Fragments arranged by size: 3197 1514 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2264 2294 2882 2912 1178 Size: 30 165 30 861 30 588 30 4207 Fragments arranged by size: 4207 861 588 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2958 3020 3124 1186 Size: 1772 62 104 4003 Fragments arranged by size: 4003 1772 104 62 BseYI C'CCAG_C Cuts at: 2525 2571 5144 5640 2525 Size: 46 2573 496 2826 Fragments arranged by size: 2826 2573 496 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2342 2733 1163 Size: 573 606 391 4371 Fragments arranged by size: 4371 606 573 391 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2825 3391 4152 5180 1540 Size: 275 186 824 566 761 1028 2301 Fragments arranged by size: 2301 1028 824 761 566 275 186 BsiHKAI G_wGCw'C Cuts at: 2367 4002 4087 5158 2367 Size: 1635 85 1071 3150 Fragments arranged by size: 3150 1635 1071 85 BsmI GAATG_Cn' Cuts at: 2749 3452 3545 2749 Size: 703 93 5145 Fragments arranged by size: 5145 703 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2004 2662 2004 Size: 658 5283 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2144 2367 2539 2559 2910 3048 3093 Size: 399 354 223 172 20 351 138 45 Cuts at: 3093 3260 4002 4087 5158 1391 Size: 167 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 354 351 223 172 167 138 85 45 20 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 3830 5560 3830 Size: 1730 4211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2508 3296 844 Size: 1664 788 3489 Fragments arranged by size: 3489 1664 788 BsrBI CCG'CTC Cuts at: 1394 1823 3828 1394 Size: 429 2005 3507 Fragments arranged by size: 3507 2005 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2704 4436 4610 65 Size: 1183 1456 1732 174 1396 Fragments arranged by size: 1732 1456 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2216 2391 2860 4585 4772 1700 Size: 110 406 175 469 1725 187 2869 Fragments arranged by size: 2869 1725 469 406 187 175 110 BsrGI T'GTAC_A Cuts at: 96 2418 96 Size: 2322 3619 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 4001 5013 4001 Size: 1012 4929 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5941 BstBI TT'CG_AA Cuts at: 2682 4745 2682 Size: 2063 3878 BstEII G'GTnAC_C Cuts at: 1184 2623 1184 Size: 1439 4502 BstYI r'GATC_y Cuts at: 1972 2703 3271 3638 4023 4040 5492 5578 5590 Size: 731 568 367 385 17 1452 86 12 Cuts at: 5590 5936 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 731 568 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2873 513 Size: 552 939 869 3581 Fragments arranged by size: 3581 939 869 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5202 Fragments arranged by size: 5202 683 56 BtsI GCAGTG_nn' Cuts at: 2025 2834 3457 4251 4271 2025 Size: 809 623 794 20 3695 Fragments arranged by size: 3695 809 794 623 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 2822 2979 Size: 54 1425 50 275 18 378 614 157 Cuts at: 2979 3388 4277 5906 8 Size: 409 889 1629 43 Fragments arranged by size: 1629 1425 889 614 409 378 275 157 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2822 3388 1537 Size: 275 1010 566 4090 Fragments arranged by size: 4090 1010 566 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2715 3871 1353 Size: 1362 1156 3423 Fragments arranged by size: 3423 1362 1156 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2174 2235 4528 4902 5048 387 Size: 1064 138 585 61 2293 374 146 1280 Fragments arranged by size: 2293 1280 1064 585 374 146 138 61 EcoICRI GAG'CTC Cuts at: 2365 2365 Size: 5941 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2027 2147 2282 2561 2682 2929 3131 Size: 393 395 120 135 279 121 247 202 Cuts at: 3131 4004 4601 5388 5656 1239 Size: 873 597 787 268 1524 Fragments arranged by size: 1524 873 787 597 395 393 279 268 247 202 135 121 120 EcoNI CCTnn'n_nnAGG Cuts at: 2720 2720 Size: 5941 EcoO109I rG'GnC_Cy Cuts at: 2140 2508 2568 3133 2140 Size: 368 60 565 4948 Fragments arranged by size: 4948 565 368 60 EcoRI G'AATT_C Cuts at: 3250 3628 3250 Size: 378 5563 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2644 2644 Size: 5941 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 2077 2088 2278 2608 5088 1678 Size: 399 11 190 330 2480 2531 Fragments arranged by size: 2531 2480 399 330 190 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4582 4614 4656 4688 1373 Size: 32 135 32 3010 32 42 32 2626 Fragments arranged by size: 3010 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3285 3531 669 Size: 988 281 1347 246 3079 Fragments arranged by size: 3079 1347 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KasI G'GCGC_C Cuts at: 2073 2084 2073 Size: 11 5930 KpnI G_GTAC'C Cuts at: 3266 3266 Size: 5941 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2085 5055 5239 1305 Size: 780 2970 184 2007 Fragments arranged by size: 2970 2007 780 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4119 Fragments arranged by size: 4119 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2861 3899 4258 4417 518 Size: 648 1695 1038 359 159 2042 Fragments arranged by size: 2042 1695 1038 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2303 2575 3125 3357 4034 5182 5427 1606 Size: 697 272 550 232 677 1148 245 2120 Fragments arranged by size: 2120 1148 697 677 550 272 245 232 NaeI GCC'GGC Cuts at: 2218 2393 2218 Size: 175 5766 NarI GG'CG_CC Cuts at: 2074 2085 2074 Size: 11 5930 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4450 Fragments arranged by size: 4450 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NgoMIV G'CCGG_C Cuts at: 2216 2391 2216 Size: 175 5766 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 3155 3299 4844 1132 Size: 97 1926 144 1545 2229 Fragments arranged by size: 2229 1926 1545 144 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5941 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5941 PfoI T'CCnGG_A Cuts at: 2259 2259 Size: 5941 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 5523 5555 4010 Size: 32 1481 32 4396 Fragments arranged by size: 4396 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2508 2568 3133 2508 Size: 60 565 5316 Fragments arranged by size: 5316 565 60 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PspOMI G'GGCC_C Cuts at: 2140 2555 2140 Size: 415 5526 PstI C_TGCA'G Cuts at: 830 2136 2302 3293 830 Size: 1306 166 991 3478 Fragments arranged by size: 3478 1306 991 166 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 PvuII CAG'CTG Cuts at: 2575 3125 2575 Size: 550 5391 SacI G_AGCT'C Cuts at: 2367 2367 Size: 5941 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SanDI GG'GwC_CC Cuts at: 2568 3133 2568 Size: 565 5376 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 4189 5901 4189 Size: 1712 4229 SfcI C'TryA_G Cuts at: 826 1045 2132 2298 2475 2590 3289 4424 5105 Size: 219 1087 166 177 115 699 1135 681 Cuts at: 5105 5296 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1087 699 681 219 191 177 166 115 SfoI GGC'GCC Cuts at: 2075 2086 2075 Size: 11 5930 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 2860 1700 Size: 1160 4781 SmaI CCC'GGG Cuts at: 1872 2240 2350 3095 3268 1872 Size: 368 110 745 173 4545 Fragments arranged by size: 4545 745 368 173 110 SmlI C'TyrA_G Cuts at: 820 1017 1943 4045 4946 5208 820 Size: 197 926 2102 901 262 1553 Fragments arranged by size: 2102 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5941 StyI C'CwwG_G Cuts at: 513 1065 2004 2115 2268 2931 3380 513 Size: 552 939 111 153 663 449 3074 Fragments arranged by size: 3074 939 663 552 449 153 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2308 2866 3968 3985 4138 4323 4768 Size: 1255 728 558 1102 17 153 185 445 Cuts at: 4768 325 Size: 1498 Fragments arranged by size: 1498 1255 1102 728 558 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2418 3220 4187 Size: 274 80 33 51 1443 441 802 967 Cuts at: 4187 5899 96 Size: 1712 138 Fragments arranged by size: 1712 1443 967 802 441 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5901 5933 5901 Size: 32 5909 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2335 2335 Size: 5941 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 2238 2348 3093 3266 1870 Size: 368 110 745 173 4545 Fragments arranged by size: 4545 745 368 173 110 XmnI GAAnn'nnTTC Cuts at: 2942 4070 2942 Size: 1128 4813 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3749 276 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI