KBTBD12 ORF clone pFN21AE0480

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Halotag ORF Clone pFN21AE0480

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Clone info.

Species

human

Clone Name

pFN21AE0480 Sequence (with vector) Restriction Map

Product ID

FHC03990

ORF Sequence

DNA sequence (1425 bp)
Protein sequence (475 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590042]

Gene

KIEE0480

Symbol

KBTBD12

Alias : KLHDC6

Description

kelch repeat and BTB (POZ) domain containing 12

Original Clone ID

oc01180

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1425 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 475 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS33848.2 2.7e-184 99.3 KBTBD12 CCDS2906.2 2.8e-32 28.5 KBTBD8 CCDS34614.1 5.6e-31 27.4 KBTBD2 CCDS33749.1 6.1e-31 27.0 KLHL18 CCDS1310.1 1.1e-29 29.1 KLHL20 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013069 23 125 PF00651 BTB/POZ IPR011705 133 234 PF07707 BTB/Kelch-associated IPR006652 378 423 PF01344 Kelch repeat type 1 IPR006652 427 446 PF01344 Kelch repeat type 1 HMMSmart IPR000210 31 128 SM00225 BTB/POZ-like IPR011705 133 235 SM00875 BTB/Kelch-associated IPR006652 386 436 SM00612 Kelch repeat type 1 ProfileScan IPR000210 31 98 PS50097 BTB/POZ-like
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 451 FPVYIIGMAILSLAAMLNIVVVV 473 PRIMARY 23 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0480 Download

>KIEE0480  1425 bp
ATGGAGTGCAAGATTGAGGGAAAAGAAAAATACCAACATAGCTTGAATTTACTGAATAAA
ATTAAGAACATGAAAGAATTAGCAGAAATGATTGATGTGGTACTCACAGCAGAAGGAGAG
AAATTTCCTTGCCACAGACTGGTCCTGGCTGCATTTAGCCCTTATTTCAAAGCTATGTTC
ACCTGTGGACTACTTGAATGTAATCAAAGGGAAGTCATACTTTATGACATCACAGCAGAA
AGTGTGTCGGTGTTATTAAATTACATGTACAATGCAGCTTTGGAGATCAATAATGCCAAT
GTACAGACTGTAGCTATGGCTGCCTATTTTATGCAGATGGAAGAAGTCTTCAGTGTGTGT
CAAAAATATATGATGGACCACATGGATGCCTCCAACTGTTTAGGTATCTATTATTTTGCA
AAGCAGATTGGAGCTGAAGATTTATCTGATCGATCAAAGAAATATTTATATCAGCACTTT
GCCGAGGTGAGCTTACATGAAGAAATACTAGAAATCGAAGTGCACCAATTTTTGACACTT
ATTAAATCAGATGATCTTAACATATCCAGAGAAGAGAGCATTCTGGACTTAGTTCTGAGA
TGGGTAAATCATAACAAAGAATTGCGTACAGTGCATCTTGTTGAGCTTTTGAAGCAAGTC
AGATTGGAACTTGTAAATCCTTCTTTTTTAAGACAAGCCCTAAGAAGGAACACAATGCTT
CTGTGTGATGCAGATTGTGTTGACATAATTCAAAATGCATTCAAAGCCATCAAGACACCC
CAACAGCACTCTCTAAATCTGCGCTATGGTATGGAGACTACCAGTCTTCTGCTTTGCATT
GGCAACAATTCTTCAGGAATCAGATCAAGACATAGGAGCTATGGGGATGCCAGTTTTTGT
TATGATCCTCTATCACGGAAAACCTATTTCATCTCATCTCCCAAGTACGGAGAGGGTTTA
GGAACTGTGTGTACTGGTGTTGTCATGGAAAATAATACTATAATTGTGGCTGGAGAAGCA
AGTGCCTCTAAACTCTCTAGACAAAAGAACAAGAATGTTGAAATTTATAGGTATCATGAT
AGAGGAAACCAGTTTTGGGAAAAGTTATGCACAGCTGAATTTCGAGAACTCTATGCTCTG
GGCAGTATTCATAATGACCTTTATGTTATAGGAGGACAGATGAAAATTAAAAACCAGTAT
CTTATTACAAACTGTGTTGATAAGTACTCTGTAGAACGGGACAATTGGAAAAGGGTGTCT
CCCCTTCCACTGCAATTGGCATGTCATGCTGTAGTGACAGTGAATAATAAACTTTATGTA
ATTGGAGGCTGGACCCCTCAGGTTAAGAAATTTCCTGTATACATAATTGGCATGGCTATA
CTGAGTCTGGCTGCTATGTTAAACATTGTTGTAGTTGTTGAATGC

ORF Protein

Cloned ORF protein sequence for pFN21AE0480 Download

>KIEE0480  475 aa
MECKIEGKEKYQHSLNLLNKIKNMKELAEMIDVVLTAEGEKFPCHRLVLAAFSPYFKAMF
TCGLLECNQREVILYDITAESVSVLLNYMYNAALEINNANVQTVAMAAYFMQMEEVFSVC
QKYMMDHMDASNCLGIYYFAKQIGAEDLSDRSKKYLYQHFAEVSLHEEILEIEVHQFLTL
IKSDDLNISREESILDLVLRWVNHNKELRTVHLVELLKQVRLELVNPSFLRQALRRNTML
LCDADCVDIIQNAFKAIKTPQQHSLNLRYGMETTSLLLCIGNNSSGIRSRHRSYGDASFC
YDPLSRKTYFISSPKYGEGLGTVCTGVVMENNTIIVAGEASASKLSRQKNKNVEIYRYHD
RGNQFWEKLCTAEFRELYALGSIHNDLYVIGGQMKIKNQYLITNCVDKYSVERDNWKRVS
PLPLQLACHAVVTVNNKLYVIGGWTPQVKKFPVYIIGMAILSLAAMLNIVVVVEC

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0480 Download

>pFN21AE0480  6130 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGTGCAAGATTGAGGGAAAAGAAAAATACCA
ACATAGCTTGAATTTACTGAATAAAATTAAGAACATGAAAGAATTAGCAGAAATGATTGA
TGTGGTACTCACAGCAGAAGGAGAGAAATTTCCTTGCCACAGACTGGTCCTGGCTGCATT
TAGCCCTTATTTCAAAGCTATGTTCACCTGTGGACTACTTGAATGTAATCAAAGGGAAGT
CATACTTTATGACATCACAGCAGAAAGTGTGTCGGTGTTATTAAATTACATGTACAATGC
AGCTTTGGAGATCAATAATGCCAATGTACAGACTGTAGCTATGGCTGCCTATTTTATGCA
GATGGAAGAAGTCTTCAGTGTGTGTCAAAAATATATGATGGACCACATGGATGCCTCCAA
CTGTTTAGGTATCTATTATTTTGCAAAGCAGATTGGAGCTGAAGATTTATCTGATCGATC
AAAGAAATATTTATATCAGCACTTTGCCGAGGTGAGCTTACATGAAGAAATACTAGAAAT
CGAAGTGCACCAATTTTTGACACTTATTAAATCAGATGATCTTAACATATCCAGAGAAGA
GAGCATTCTGGACTTAGTTCTGAGATGGGTAAATCATAACAAAGAATTGCGTACAGTGCA
TCTTGTTGAGCTTTTGAAGCAAGTCAGATTGGAACTTGTAAATCCTTCTTTTTTAAGACA
AGCCCTAAGAAGGAACACAATGCTTCTGTGTGATGCAGATTGTGTTGACATAATTCAAAA
TGCATTCAAAGCCATCAAGACACCCCAACAGCACTCTCTAAATCTGCGCTATGGTATGGA
GACTACCAGTCTTCTGCTTTGCATTGGCAACAATTCTTCAGGAATCAGATCAAGACATAG
GAGCTATGGGGATGCCAGTTTTTGTTATGATCCTCTATCACGGAAAACCTATTTCATCTC
ATCTCCCAAGTACGGAGAGGGTTTAGGAACTGTGTGTACTGGTGTTGTCATGGAAAATAA
TACTATAATTGTGGCTGGAGAAGCAAGTGCCTCTAAACTCTCTAGACAAAAGAACAAGAA
TGTTGAAATTTATAGGTATCATGATAGAGGAAACCAGTTTTGGGAAAAGTTATGCACAGC
TGAATTTCGAGAACTCTATGCTCTGGGCAGTATTCATAATGACCTTTATGTTATAGGAGG
ACAGATGAAAATTAAAAACCAGTATCTTATTACAAACTGTGTTGATAAGTACTCTGTAGA
ACGGGACAATTGGAAAAGGGTGTCTCCCCTTCCACTGCAATTGGCATGTCATGCTGTAGT
GACAGTGAATAATAAACTTTATGTAATTGGAGGCTGGACCCCTCAGGTTAAGAAATTTCC
TGTATACATAATTGGCATGGCTATACTGAGTCTGGCTGCTATGTTAAACATTGTTGTAGT
TGTTGAATGCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT