(Circular) (Six-base) MAPSORT of: pFN21AE0480.seq Check: 756 from: 1 to: 6130 pFN21AE0480 6130 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3940 278 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3363 3473 1937 Size: 1426 110 4594 Fragments arranged by size: 4594 1426 110 Acc65I G'GTAC_C Cuts at: 3451 3451 Size: 6130 AclI AA'CG_TT Cuts at: 1563 4257 4630 1563 Size: 2694 373 3063 Fragments arranged by size: 3063 2694 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2338 2460 2841 4193 5577 5845 1632 Size: 706 122 381 1352 1384 268 1917 Fragments arranged by size: 1917 1384 1352 706 381 268 122 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5933 AflIII A'CryG_T Cuts at: 1203 2268 5029 6006 1203 Size: 1065 2761 977 1327 Fragments arranged by size: 2761 1327 1065 977 AgeI A'CCGG_T Cuts at: 4961 4961 Size: 6130 AhdI GACnn_n'nnGTC Cuts at: 2826 4859 2826 Size: 2033 4097 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6130 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6130 AlwNI CAG_nnn'CTG Cuts at: 3873 5445 3873 Size: 1572 4558 ApaLI G'TGCA_C Cuts at: 2525 4187 5343 2525 Size: 1662 1156 3312 Fragments arranged by size: 3312 1662 1156 ApoI r'AATT_y Cuts at: 1493 2050 2126 3066 3122 3353 3439 3665 3817 Size: 557 76 940 56 231 86 226 152 Cuts at: 3817 5926 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 940 557 231 226 152 86 76 56 AseI AT'TA_AT Cuts at: 160 1032 4684 160 Size: 872 3652 1606 Fragments arranged by size: 3652 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 AvaI C'yCGr_G Cuts at: 1870 1943 3455 1870 Size: 73 1512 4545 Fragments arranged by size: 4545 1512 73 BamHI G'GATC_C Cuts at: 3460 3827 3460 Size: 367 5763 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3451 4906 618 Size: 325 217 627 1664 1455 1842 Fragments arranged by size: 1842 1664 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3449 1391 Size: 2058 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2344 2822 928 Size: 971 445 478 4236 Fragments arranged by size: 4236 971 478 445 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4321 4355 1903 Size: 34 2384 34 3678 Fragments arranged by size: 3678 2384 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4022 5232 4022 Size: 1210 4920 BclI T'GATC_A Cuts at: 1548 1548 Size: 6130 BfrBI ATG'CAT Cuts at: 2762 2762 Size: 6130 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4741 136 Size: 107 122 71 631 605 3069 1525 Fragments arranged by size: 3069 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6125 6125 Size: 6130 BlpI GC'TnA_GC Cuts at: 3547 3547 Size: 6130 Bme1580I G_kGCm'C Cuts at: 1790 2529 4191 5347 1790 Size: 739 1662 1156 2573 Fragments arranged by size: 2573 1662 1156 739 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6130 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3861 4819 442 Size: 416 53 83 399 24 2444 958 1753 Fragments arranged by size: 2444 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3036 4790 1239 Size: 1797 1754 2579 Fragments arranged by size: 2579 1797 1754 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4255 5120 5418 4255 Size: 865 298 4967 Fragments arranged by size: 4967 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4793 882 Size: 617 3294 2219 Fragments arranged by size: 3294 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3921 493 Size: 711 2717 2702 Fragments arranged by size: 2717 2702 711 BsaBI GATnn'nnATC Cuts at: 6124 6124 Size: 6130 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3937 4319 4954 275 Size: 53 83 186 936 2404 382 635 1451 Fragments arranged by size: 2404 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4563 4961 5235 5382 5793 4563 Size: 398 274 147 411 4900 Fragments arranged by size: 4900 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5905 Fragments arranged by size: 5905 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6130 BseYI C'CCAG_C Cuts at: 5333 5829 5333 Size: 496 5634 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5557 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3580 4341 5369 1540 Size: 275 186 1579 761 1028 2301 Fragments arranged by size: 2301 1579 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2529 4191 4276 5347 2529 Size: 1662 85 1071 3312 Fragments arranged by size: 3312 1662 1071 85 BsmI GAATG_Cn' Cuts at: 2583 2762 3431 3641 3734 2583 Size: 179 669 210 93 4979 Fragments arranged by size: 4979 669 210 179 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2529 3449 4191 4276 5347 1391 Size: 399 739 920 742 85 1071 2174 Fragments arranged by size: 2174 1071 920 742 739 399 85 BspEI T'CCGG_A Cuts at: 5793 5793 Size: 6130 BspHI T'CATG_A Cuts at: 3079 4019 5749 3079 Size: 940 1730 3460 Fragments arranged by size: 3460 1730 940 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3485 844 Size: 2641 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4017 1394 Size: 429 2194 3507 Fragments arranged by size: 3507 2194 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4625 4799 65 Size: 1183 3377 174 1396 Fragments arranged by size: 3377 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4774 4961 1700 Size: 110 2964 187 2869 Fragments arranged by size: 2964 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2271 2305 96 Size: 2175 34 3921 Fragments arranged by size: 3921 2175 34 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6130 BssSI C'ACGA_G Cuts at: 4190 5202 4190 Size: 1012 5118 BstAPI GCAn_nnn'nTGC Cuts at: 794 3290 794 Size: 2496 3634 BstBI TT'CG_AA Cuts at: 4934 4934 Size: 6130 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6130 BstYI r'GATC_y Cuts at: 1972 3460 3827 4212 4229 5681 5767 5779 6125 Size: 1488 367 385 17 1452 86 12 346 Cuts at: 6125 1972 Size: 1977 Fragments arranged by size: 1977 1488 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3364 3364 Size: 6130 Bsu36I CC'TnA_GG Cuts at: 3342 3342 Size: 6130 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4639 Fragments arranged by size: 4639 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5391 Fragments arranged by size: 5391 683 56 BtsI GCAGTG_nn' Cuts at: 3272 3646 4440 4460 3272 Size: 374 794 20 4942 Fragments arranged by size: 4942 794 374 20 ClaI AT'CG_AT Cuts at: 1554 2455 1554 Size: 901 5229 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3261 3296 529 Size: 35 2697 35 3363 Fragments arranged by size: 3363 2697 35 35 DraI TTT'AAA Cuts at: 3434 3434 Size: 6130 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5137 809 Size: 507 3821 1802 Fragments arranged by size: 3821 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3577 4466 6095 Size: 54 1425 50 275 18 1747 889 1629 Cuts at: 6095 8 Size: 43 Fragments arranged by size: 1747 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3577 1537 Size: 275 1765 4090 Fragments arranged by size: 4090 1765 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2571 4060 1353 Size: 1218 1489 3423 Fragments arranged by size: 3423 1489 1218 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4717 5091 5237 387 Size: 1064 138 3128 374 146 1280 Fragments arranged by size: 3128 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2338 2460 2841 3036 4193 4790 5577 Size: 393 706 122 381 195 1157 597 787 Cuts at: 5577 5845 1239 Size: 268 1524 Fragments arranged by size: 1524 1157 787 706 597 393 381 268 195 122 EcoRI G'AATT_C Cuts at: 3439 3817 3439 Size: 378 5752 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2240 2240 Size: 6130 FspI TGC'GCA Cuts at: 4636 4636 Size: 6130 HaeII r_GCGC'y Cuts at: 1678 5277 1678 Size: 3599 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2812 2844 2900 2932 3215 Size: 32 135 32 1240 32 56 32 283 Cuts at: 3215 3247 4771 4803 4845 4877 1373 Size: 32 1524 32 42 32 2626 Fragments arranged by size: 2626 1524 1240 283 135 56 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2746 3474 3720 669 Size: 988 281 808 728 246 3079 Fragments arranged by size: 3079 988 808 728 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6130 HpaI GTT'AAC Cuts at: 3720 3720 Size: 6130 KpnI G_GTAC'C Cuts at: 3455 3455 Size: 6130 MfeI C'AATT_G Cuts at: 3247 3278 3729 3247 Size: 31 451 5648 Fragments arranged by size: 5648 451 31 MluI A'CGCG_T Cuts at: 6006 6006 Size: 6130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2421 5244 5428 1305 Size: 1116 2823 184 2007 Fragments arranged by size: 2823 2007 1116 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4308 Fragments arranged by size: 4308 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2388 4088 4447 4606 518 Size: 648 1222 1700 359 159 2042 Fragments arranged by size: 2042 1700 1222 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3119 3546 4223 5371 5616 1606 Size: 1513 427 677 1148 245 2120 Fragments arranged by size: 2120 1513 1148 677 427 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4639 Fragments arranged by size: 4639 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6130 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6130 NotI GC'GGCC_GC Cuts at: 3577 3577 Size: 6130 NsiI A_TGCA'T Cuts at: 2764 2764 Size: 6130 NspI r_CATG'y Cuts at: 1132 1229 2272 3288 3488 5033 1132 Size: 97 1043 1016 200 1545 2229 Fragments arranged by size: 2229 1545 1043 1016 200 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6130 PciI A'CATG_T Cuts at: 2268 5029 2268 Size: 2761 3369 PflMI CCAn_nnn'nTGG Cuts at: 1108 2143 1108 Size: 1035 5095 PmeI GTTT'AAAC Cuts at: 3434 3434 Size: 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4199 4231 5712 5744 4199 Size: 32 1481 32 4585 Fragments arranged by size: 4585 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6130 PsiI TTA'TAA Cuts at: 3700 3700 Size: 6130 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2959 2991 2959 Size: 32 6098 PstI C_TGCA'G Cuts at: 830 3482 830 Size: 2652 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6130 PvuII CAG'CTG Cuts at: 3119 3119 Size: 6130 SalI G'TCGA_C Cuts at: 1936 3472 1936 Size: 1536 4594 SbfI CC_TGCA'GG Cuts at: 3482 3482 Size: 6130 ScaI AGT'ACT Cuts at: 3230 4378 6090 3230 Size: 1148 1712 3270 Fragments arranged by size: 3270 1712 1148 SfcI C'TryA_G Cuts at: 826 1045 2313 3234 3294 3478 4613 5294 5485 Size: 219 1268 921 60 184 1135 681 191 Cuts at: 5485 826 Size: 1471 Fragments arranged by size: 1471 1268 1135 921 681 219 191 184 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6130 SmaI CCC'GGG Cuts at: 1872 3457 1872 Size: 1585 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4234 5135 5397 820 Size: 197 926 2291 901 262 1553 Fragments arranged by size: 2291 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3921 493 Size: 3428 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6130 SphI G_CATG'C Cuts at: 1132 3488 1132 Size: 2356 3774 SspI AAT'ATT Cuts at: 5 52 2468 4054 5 Size: 47 2416 1586 2081 Fragments arranged by size: 2416 2081 1586 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6130 StyI C'CwwG_G Cuts at: 513 1065 2004 3569 513 Size: 552 939 1565 3074 Fragments arranged by size: 3074 1565 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4157 4174 4327 4512 4957 325 Size: 1255 2577 17 153 185 445 1498 Fragments arranged by size: 2577 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2271 2305 2975 Size: 274 80 33 51 1443 294 34 670 Cuts at: 2975 3228 4376 6088 96 Size: 253 1148 1712 138 Fragments arranged by size: 1712 1443 1148 670 294 274 253 138 80 51 34 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6090 6122 6090 Size: 32 6098 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6130 XbaI T'CTAG_A Cuts at: 3041 3466 3041 Size: 425 5705 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6130 XmaI C'CCGG_G Cuts at: 1870 3455 1870 Size: 1585 4545 XmnI GAAnn'nnTTC Cuts at: 4259 4259 Size: 6130 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3938 276 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI